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pMRX-bsr-EGFP-hRab27A-V143A

Retroviral expression vector of human Rab27A with a mutation (V143A) that disrupts Munc13-4 binding.

Catalog number RDB18648
Resource name pMRX-bsr-EGFP-hRab27A-V143A
Clone info. Retroviral expression vector of human Rab27A mutant (V143A) tagged with EGFP at N-terminus. A new GS2 mutation that disrupts Munc13-4 binding.
Comment Expression was confirmed by the depositor with immunoblotting.
Vector backbone pMRX-IRES-bsr-EGFP (plasmid )
Selectable markers Ampicillin (E. coli), Blasticidin (mammalian cell)
Gene/insert name Human RAB27A cDNA
Depositor|Developer Fukuda, Mitsunori |
Other clones in our bank

External Database
Human RAB27A

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Aizawa, M. and Fukuda, M. J. Biol. Chem. 290, 22250-22261, 2015; Ohishi, Y. et al. Front. Immunol. 11, 612977, 2020)
          Additional terms and conditions for distribution Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested.
          Ordering Please visit Information of Request for Distribution.[link] 
          Order form 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
          Remarks Remember that you will be working with samples containing infectious virus.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Aizawa, M. and Fukuda, M. J. Biol. Chem. 290, 22250-22261, 2015; Ohishi, Y. et al. Front. Immunol. 11, 612977, 2020)。
          付加的提供条件 pMRXベクターについて:利用者は、研究成果の公表にあたって pMXならびにpMRXベクターの文献 (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) の引用を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。
          備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB18648 pMRX-bsr-EGFP-hRab27A-V143A Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Electronic file

          Sequence (partial) RDB18648pka01.seq provided by the depositor

          Original reference

          original Ohishi, Y., Griscelli Syndrome Type 2 Sine Albinism: Unraveling Differential RAB27A Effector Engagement. Front. Immunol. 11: 612977 (2020). PMID 33362801.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


          References

          Original, user report and related articles

          original Ohishi, Y., Griscelli Syndrome Type 2 Sine Albinism: Unraveling Differential RAB27A Effector Engagement. Front. Immunol. 11: 612977 (2020). PMID 33362801.
          reference Aizawa, M., Small GTPase Rab2B and Its Specific Binding Protein Golgi-associated Rab2B Interactor-like 4 (GARI-L4) Regulate Golgi Morphology. J. Biol. Chem. 290 (36): 22250-22261 (2015). PMID 26209634.

          2022.05.16

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