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pMRX-bsr-EGFP-hRab27A-V143A

Retroviral expression vector of human Rab27A with a mutation (V143A) that disrupts Munc13-4 binding.

Catalog number RDB18648
Resource name pMRX-bsr-EGFP-hRab27A-V143A
Clone info. Retroviral expression vector of human Rab27A mutant (V143A) tagged with EGFP at N-terminus. A new GS2 mutation that disrupts Munc13-4 binding.
Expression was confirmed by the depositor with immunoblotting.
Vector backbone pMRX-IRES-bsr-EGFP (plasmid )
Selectable markers Ampicillin (E. coli), Blasticidin (mammalian cell)
Gene/insert name Human RAB27A cDNA
Depositor|Developer Fukuda, Mitsunori |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Aizawa, M. and Fukuda, M. J. Biol. Chem. 290, 22250-22261, 2015; Ohishi, Y. et al. Front. Immunol. 11, 612977, 2020)
Additional terms and conditions for distribution Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested.
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Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Schedule A (GFP-A) [Link]
Remarks Remember that you will be working with samples containing infectious virus.
Please use Schedule A (GFP-A) additionally.
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Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB18648 pMRX-bsr-EGFP-hRab27A-V143A Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Nucleotide sequence (partial) provided by the depositor RDB18648pka01.seq

Original reference

original Ohishi, Y., Griscelli Syndrome Type 2 Sine Albinism: Unraveling Differential RAB27A Effector Engagement. Front. Immunol. 11: 612977 (2020). PMID 33362801.

Further references such as user reports and related articles (go to bottom)

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Sequence information

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


References

Original, user report and related articles

original Ohishi, Y., Griscelli Syndrome Type 2 Sine Albinism: Unraveling Differential RAB27A Effector Engagement. Front. Immunol. 11: 612977 (2020). PMID 33362801.
reference Aizawa, M., Small GTPase Rab2B and Its Specific Binding Protein Golgi-associated Rab2B Interactor-like 4 (GARI-L4) Regulate Golgi Morphology. J. Biol. Chem. 290 (36): 22250-22261 (2015). PMID 26209634.

2021.02.05

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