Resource data sheet
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pMRX-bsr-EGFP-hRab27A (#RDB18647)

Retroviral expression vector of human Rab27A.

Clone info. Retroviral expression vector of human Rab27A tagged with EGFP at N-terminus.
Comment Expression was confirmed by the depositor with immunoblotting.
Vector backbone pMRX-IRES-bsr-EGFP (plasmid )
Selectable markers Ampicillin (E. coli), Blasticidin (mammalian cell)
Gene/insert name Human RAB27A cDNA
Depositor|Developer Fukuda, Mitsunori |
 
Sequence (partial) RDB18647pka01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Aizawa, M. and Fukuda, M. J. Biol. Chem. 290, 22250-22261, 2015; Ohishi, Y. et al. Front. Immunol. 11, 612977, 2020)
Additional terms and conditions:
Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested.
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Aizawa, M. and Fukuda, M. J. Biol. Chem. 290, 22250-22261, 2015; Ohishi, Y. et al. Front. Immunol. 11, 612977, 2020)。
MTAに書く付加的使用条件：
pMRXベクターについて：利用者は、研究成果の公表にあたって pMXならびにpMRXベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) の引用を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB18647 pMRX-bsr-EGFP-hRab27A Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pMRX-bsr-EGFP-hRab27A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18647).

Reference section:

Ohishi, Y., Ammann, S., Ziaee, V., Strege, K., Groß, M., Amos, C.V., Shahrooei, M., Ashournia, P., Razaghian, A., Griffiths, G.M., Ehl, S., Fukuda, M., Parvaneh, N., Griscelli Syndrome Type 2 Sine Albinism: Unraveling Differential RAB27A Effector Engagement. Front. Immunol. 11: 612977 (2020). PMID 33362801. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Ohishi, Y., Griscelli Syndrome Type 2 Sine Albinism: Unraveling Differential RAB27A Effector Engagement. Front. Immunol. 11: 612977 (2020). PMID 33362801. [link to RRC of NBRP]
reference Aizawa, M., Small GTPase Rab2B and Its Specific Binding Protein Golgi-associated Rab2B Interactor-like 4 (GARI-L4) Regulate Golgi Morphology. J. Biol. Chem. 290 (36): 22250-22261 (2015). PMID 26209634. [link to RRC of NBRP]