RDB17897 - spret MACS1-luc2

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icon below as well as clone information before placing your order.

spret MACS1-luc2 (#RDB17897)

Luciferase reporter of the Western Mediterranean mouse (Mus spretus) Shh enhancer, MACS1.

Clone info.	Luciferase reporter of the Western Mediterranean mouse (Mus spretus) Sonic hedgehog (Shh) enhancer, MACS1.
Comment	Expression was confirmed by the depositor with luciferase assay.
Vector backbone	pGL4.23 (plasmid)
Size of vector backbone	4.3 kb
Selectable markers	Ampicillin
Gene/insert name	mouse Shh genomic
Depositor\|Developer	Amano, Takanori \|

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Tsukiji N. et al., Mech. Dev. 131:127-36, 2014)
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Tsukiji N. et al., Mech. Dev. 131:127-36, 2014) Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Tsukiji N. et al., Mech. Dev. 131:127-36, 2014)。 MTAに書く付加的使用条件：本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB17897	spret MACS1-luc2	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The spret MACS1-luc2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17897).

Reference section:

Tsukiji, N., Amano, T., Shiroishi, T., A novel regulatory element for Shh expression in the lung and gut of mouse embryos. Mech. Dev. 131: 127-136 (2014). PMID 24157522. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB17897_B0Afp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4ins_F (Pr0665) Region: Pause site, Spret MACS1 5' Sequence file: RDB17897_B0Afa.seq
>D06932A2_B0Af_2_pGL4ins_F_D10_10_ABI24.ab1 1 NNNNNNNNNN NNNNNNACNN GCNNATAGGC TGTCCCCNGT GCAAGTGCAG GTGCCAGAAC 61 ATTTCTCTGG CCTAACTGGC CGGTACCTGA GCTCGCTAGC CTCGAGATAA CCAGGAGTCC 121 AGGTACTTGA TATGTAAAGC AATTAGTGGT CATTTGAAAA CAGTACACTG GCTAAAAGTT 181 AAAAAAATAC TAACAAAAGC ATTTAGGTGA ACTCTGTCTG CTCAGGAGAG TCCTAAGTGA 241 TCCATCTAAT GTCAGCAAAT TGCTTACTAA TGTGTTGACT TAGGGCCACC CTCAAAAGCA 301 AAATGAAAAG CCTGGAATTT ATAGCATCTC AATGCATCAG CTGATAAGCT TTTACTGGTT 361 TAATATCCTT CCAGGCTTGC AGCTTGGAGC CACTTCAAGT TAGCAACAGA ATGTTTGCCA 421 TCTCTATGTG ATAAAGGAAG TCAAAGGGAT GAACAAACTT CAGGACCATT ATGCTGTTTG 481 GTGAGATGGA AATGAAAAAG CTAGAGAAGT CACGGGTTGG GGGTGTGTGA GAGCTGGGCC 541 TTCAAGTCCG GCCTCTGACG GATTGTTAGC CTCACCCCTC AAACAATGGT CTGGACTTTA 601 GCTGGAGCAG AGATAACATA GTGCATTTGG AAAGTTTTAA TTGCAGCAAT AAAAGACTAT 661 GCCTCAATTA TAGGACAGAT GCTAGCAAAG TTTCCAGATA AGGATCAAAG GCACCTAAGG 721 AGTGGCCACT GCACTTTGGG ACACACTAGG CTTTCTGTAC ACACTGCTGG GGAATTAGCA 781 CATCAGCTTT CCGACCTTTC TAGGTCCTTT GATCTGAGTT CCCAAGCAAG TAAGTGTATC 841 AAGANTACTG TATCAGCCAG ACAGGTCCNA CACTCCCTTG CTGAATTTNN ATCCTGANAC 901 TCACNAGNAC AAAACCNAAC ACCTGANCTT NNCAGTTCNN ANCACNAAAG TTCAGNGNGC 961 TANNNCNNGC CNCNGNGGNC NANCTNANAN NCNNNAGGGN ATNNNNNNNN NN //
Primer: Luc_R (Pr0146) Region: luc2 5', mini P, Spret MACS1 3' Sequence file: RDB17897_B0Afb.seq
>D06932A2_B0Af_2_Luc_R_E10_13_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN GNAGCGCTTC NTGGCTTTGT GCNGCTGCTC GCCGGCGGTC 61 CCGTCTTCGA GTGGGTAGAA TGGCGCTGGG CCCTTCTTAA TGTTTTTGGC ATCTTCCATG 121 GTGGCTTTAC CAACAGTACC GGATTGCCAA GCTGGAAGTC GAGCTTCCAT TATATACCCT 181 CTAGTGTCTA AGCTTGGCCG CCGAGGCCAG ATCTAGCACA CTGAACTTTT GTGGTAAGAA 241 CTGCAAAGGT CAGGTGTTAG GTTTTGTACT GGTGAGTGTC AGGATTAAAA TTCAGCAAGG 301 GAGTGTTGGA CCTGTCTGGC TGATACAGTA GTCTTGATAC ACTTACTTGC TTGGGAACTC 361 AGATCAAAGG ACCTAGAAAG GTCGGAAAGC TGATGTGCTA ATTCCCCAGC AGTGTGTACA 421 GAAAGCCTAG TGTGTCCCAA AGTGCAGTGG CCACTCCTTA GGTGCCTTTG ATCCTTATCT 481 GGAAACTTTG CTAGCATCTG TCCTATAATT GAGGCATAGT CTTTTATTGC TGCAATTAAA 541 ACTTTCCAAA TGCACTATGT TATCTCTGCT CCAGCTAAAG TCCAGACCAT TGTTTGAGGG 601 GTGAGGCTAA CAATCCGTCA GAGGCCGGAC TTGAAGGCCC AGCTCTCACA CACCCCCAAC 661 CCGTGACTTC TCTAGCTTTT TCATTTCCAT CTCACCAAAC AGCATAATGG TCCTGAAGTT 721 TGTTCATCCC TTTGACTTCC TTTATCACAT AGAGATGGCA AACATTCTGT TGCTAACTTG 781 AAGTGGCTCC NAGCTGCAAG CCTGGAANGA TATTAAACCA GTAAAAGCTT ATCAGCTGAN 841 GCATTGAGAT GCTATAAATT CCAGGCTTTT CATTTTGCTT TTGANGGTGG NCCTAANTCA 901 ACACATTAGN AAGCAATTTG CTGANNTTAN NNGGNTCACT NANGANTCTC CTGANCANAN 961 AGANTTCNNC TAANNGNTTT TGNTANTATT TTTTTNNCTT TTANCCNGNG NNNNGNTTTN 1021 NNNGANNNCT NNNNGNNTNN CNNNNNNNNN N //
Primer: pGL4pA_R (Pr0664) Region: SV40 late p(A), luc2 3' Sequence file: RDB17897_B0Afc.seq
>D06932A2_B0Af_2_pGL4pA_R_F10_16_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNC NTNNTCGANT TTNCNNNNTT TGTAGAGGTT TTACTTGCTT 61 TAAAAAACCT CCCACACCTC CCCCTGAACC TGAAACATAA AATGAATGCA ATTGTTGTTG 121 TTAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA 181 CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT 241 CTTATCATGT CTGCTCGAAG CGGCCGGCCG CCCCGACTCT AGAATTATTA CACGGCGATC 301 TTGCCGCCCT TCTTGGCCTT AATGAGAATC TCGCGGATCT TGCGGGCGTC CAACTTGCCG 361 GTCAGTCCTT TAGGCACCTC GTCCACGAAC ACAACACCAC CGCGCAGCTT CTTGGCGGTT 421 GTAACCTGGC TGGCCACATA GTCCACGATC TCCTTCTCGG TCATGGTTTT ACCGTGTTCC 481 AGCACGACGA CTGCGGCGGG CAGCTCGCCG GCATCGTCGT CGGGCAGGCC GGCGACCCCG 541 GCGTCGAAGA TGTTGGGGTG TTGCAGCAGG ATGCTCTCCA GTTCGGCTGG GGCTACCTGG 601 TAGCCCTTGT ATTTGATCAG GCTCTTCAGC CGGTCCACGA TGAAGAAGTG CTCGTCCTCG 661 TCCCAGTAGG CGATGTCGCC GCTGTGCAGC CAGCCGTCCT TGTCGATGAG AGCGTTTGTA 721 GCCTCGGGGT TGTTAACGTA GCCGCTCATG ATCATGGGGC CACGGACGCA CAGCTCGCCG 781 CGCTGGTTCA CACCCAGTGT CTTACCGGTG TCCAAGTCCA CCACCTTAGC CTCGAANANN 841 GGCACCACCT TGCCNACTGC GCCAGGCTTG TCGTCCCNTT CNGGGGNGAN NANANNGNNN 901 CNNNNTGNTN CTGNCANGNN GTANCCCTGN CGGANGNCTG GNNNGNGNNA NCGTTTGGNN 961 NNNNGNNNTC NNNTNNNNNC NNTGNNNANC NGNGCCCCGN NNNNNGNNGA NNNNNNGNNN 1021 GTNGNNTNNN NTCNNANNTN NNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Tsukiji, N., A novel regulatory element for Shh expression in the lung and gut of mouse embryos. Mech. Dev. 131: 127-136 (2014). PMID 24157522. [link to RRC of NBRP]

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mouse Shh

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mouse Shh

Reference sequence