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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEBMulti-Neo-human-CDC123 (#RDB17728)

Expression vector of human cell division cycle 123 (CDC123).

Alternative name CDC123
Clone info. Expression vector of human cell division cycle 123 (CDC123) cloned into the pEBMulti-Neo.
Comment Expression was confirmed by the depositor.
Vector backbone pEBMulti-Neo (plasmid)
Size of vector backbone 10.0 kb
Selectable markers Kanamycin (E. coli), Neomycin (mammalian cells).
Gene/insert name human CDC123 cDNA
Depositor|Developer Ito, Takuhiro |
 
Sequence (partial) RDB17728pka01.seq provided by the depositor

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kashiwagi, K. et al. Science, 364(6439), 495-499, 2019).
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kashiwagi, K. et al. Science, 364(6439), 495-499, 2019)。

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RDB17728 pEBMulti-Neo-human-CDC123 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEBMulti-Neo-human-CDC123 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17728).

Reference section:

Kashiwagi, K., Yokoyama, T., Nishimoto, M., Takahashi, M., Sakamoto, A., Yonemochi, M., Shirouzu, M., Ito, T., Structural basis for eIF2B inhibition in integrated stress response. Science 364 (6439): 495-499 (2019). PMID 31048492. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17728_B0Jup1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pCAG-F_V3 (Pr0495)
Region: inser 5'
Sequence file: RDB17728_B0Jua.seq check
>D06721A7_B0Ju_3-3_pCAG-F_V3_A07_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNCANGNC GGGGTTCGGC TTCTGGNNNG
   61 TGANCGGCGG CTNNNNNNNN NNNGNNNNCC ATGTTCATGC CTTCTTCTTT TTCCTACAGC
  121 TCCTGGGCAA CGTGCTGGTT ATTGTGCTGT CTCATCATTT TGGCAAAGAA TTAACCCTCA
  181 CTAAAGGGGT ACCGGGCCCC CCCTCGAGGT CGACGCCACC ATGAAGAAGG AGCATGTGCT
  241 TCACTGCCAG TTCTCCGCGT GGTACCCGTT CTTCCGAGGC GTTACCATCA AGAGTGTCAT
  301 TCTTCCACTT CCTCAGAATG TGAAGGATTA TTTACTCGAT GATGGAACTC TGGTGGTTTC
  361 AGGAAGGGAT GATCCACCAA CACATTCTCA GCCAGACAGT GATGATGAAG CAGAAGAAAT
  421 ACAGTGGTCT GATGATGANA ACNCAGCCAC GCTTACGGCA CCAGAATTTC CTGAGTTTGC
  481 CACTAAAGTC CAGGAAGCTA TCAATTCCCT CGGGGGCAGT GTCTTTCCTA AGCTTAATTG
  541 GAGTGCCCCA AGGGATGCGT ATTGGATAGC AATGAATAGT TCTCTGAAAT GTAAAACCCT
  601 CAGCGACATC TTTCTGCTTT TCAAGAGTTC CGATTTCNTN NNNCGTGACT TCNNTCNNCC
  661 GTTTATTCNT TGTACTGATG ATTCTCCNNA TCCATGTATA GAATATGAGC TCGTTCTCCG
  721 AAAATGGNGT GAATTGATTC NNNGGGGCTG AGTTTCNATG TTTTGTCAAG GAAAACNAGC
  781 TTANTGGTAT TTCTCNAAGA GANTACNCAC NATACTATGN ATCNNNNTNT CTAANCAAAA
  841 GNNAANAANN TNCGCANANG CATANCAANN NTTTTTTCNA NGNAACANAT NNNNNACANA
  901 NTNNNNNNNA TGAANANTTT NNTGNTNNNA NANNTANNNA NACNNNNNNG GGGAANGNGN
  961 GGNNNNNTNN NNTTTNANNN CNNNTNGNNN NANNNNNNAN NNTNNNNNTG NNNNNNNNNC
1021 NNNGNNAGNA NNNGNNNNNN NNNN
//
Primer: SV40polyA_R2 (Pr0623)
Region: SV40 pA, insert 3'
Sequence file: RDB17728_B0Jub.seq check
>D06721A7_B0Ju_3-3_SV40polyA_R2_B07_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN ACAATTGCAT TCNTTTTATG
   61 TTTCNGGTTC AGGGGGAGNN NNNNNNGGTT TTNNNNNNCA AGTAAAACCT CTACAAATGT
  121 GGTATGGCTG ATTATGATCT AGAGTCGCGG CCGCTCAGTC GTCCTCCTGC TGATTTCTCT
  181 TCAGCTTAAG GAAGTCTATT AGCTTGTGAG CGTCCTCCCC AGTAGAGAGG TCTACAAAGT
  241 CCTTGGGTAG CCGGTAACTC AAATAGGGGC TGGGCTGGAC TGTCACTTCA CTGTTTGTGC
  301 AACGGAAAGC TGGGGAATCC TGCTCTTGAG CGTCAACTTC ACTAAAATCG CCGTTTAAGT
  361 TGTTCTCAGA TATCAGTTCT TCCCAGGTGA ACAGCAGTGA ATCTGTGACT TCACCAAATG
  421 GATTAAAGTC AATGAGCCAC ACCTTCCCCC TACTGTCTCT GTATATATCG AACACAAAGT
  481 CTTCATCTAA GAATTTGTAC TGTATGTGTT TCTTGAAAAA GTCTTGTATG CATCTGCGAA
  541 TTTCTTCCTT TTGTTTAGAA ATATGATCAT AGTATTGTGT GTAGTCTCTT TGAGAAATAC
  601 CAATAAGCTT GTTTTCCTTG ACAAAACATC GAAACTCAGC CCCAGGAATC NATTCACACC
  661 ATTTTCGGAG AACGAGCTCA TATTCTATAC ATGGATCTGG AGAATCNNNN NNACAATGAA
  721 TAAACGGCTG ANTGAAGNNN NNAGTGATGA ANTCGGAACT CTTGAAAAAG CAGAAAAGAN
  781 GTCNNTGANG NNTTTACATT TCNNANANCT ATTCATTGCT ATCCNATACN CATCCCTTGG
  841 GGCACTNCNA TTANNCTTAN GAAAGANACT GNCCCCCNAN GNNNNNNNAT AGCTTCCTGG
  901 ACTTTANTGG CAAACTNNNN AAANTNCNGG NNGNCGNAAG CNNGGNNTGN GGTNCTCNTC
  961 NTCANANCNN NNNNATTTTC NTNCNNGNNT NNNNNNNNNC ACNNGNNTGN NNNANNNNNN
1021 NNTTNNNNGN AATNNNNNNC NTTNNNCNNN NAANCNNCNN NNANNTNNNN NNNNNN
//
Primer: oriP_F (Pr0765)
Region: EBNA-1
Sequence file: RDB17728_B0Juc.seq check
>D06721A7_B0Ju_3-3_oriP_F_D07_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NGGGGNNNNN NNNNTTCGCN GCTNGTCCTT
   61 TTCCTGCTGG TTGCTCCCNN NNNNNNNNNN ATTTAAGGAG GCCAGGCTAA AGCCGTCGCA
  121 TGTCTGATTG CTCACCAGGT AAATGTCGCT AATGTTTTCC AACGCGAGAA GGTGTTGAGC
  181 GCGGAGCTGA GTGACGTGAC AACATGGGTA TGCCCAATTG CCCCATGTTG GGAGGACGAA
  241 AATGGTGACA AGACAGATGG CCAGAAATAC ACCAACAGCA CGCATGATGT CTACTGGGGA
  301 TTTATTCTTT AGTGCGGGGG AATACACGGC TTTTAATACG ATTGAGGGCG TCTCCTAACA
  361 AGTTACATCA CTCCTGCCCT TCCTCACCCT CATCTCCATC ACCTCCTTCA TCTCCGTCAT
  421 CTCCGTCATC ACCCTCCGCG GCAGCCCCTT CCACCATAGG TGGAAACCAG GGAGGCAAAT
  481 CTACTCCATC GTCAAAGCTG CACACAGTCA CCCTGATATT GCAGGTAGGA GCGGGCTTTG
  541 TCATAACAAG GTCCTTAATC GCATCCTTCA AAACCTCAGC AAATATATGA GTTTGTAAAA
  601 AGACCATGAA ATAACNNACA ATGGACTCCC TTANCGGGCC AGGTTGTGGG CCGGGTCCAG
  661 GGNNNNNNCC NAAGGGGAGA CGACTCNANT GGTGTAAGAC GACATTGTGG AATAGCNANG
  721 GGCNGTTCCT CGCCTTANGT TGTAAAGGGA GGTCTTACTA CCTCCNTATA CNAACACANC
  781 GGCNANCCAA GTTCCTTCGN CGGNAGTCCT TTCTANNTGA CTCCTANNCN NNNANAGCTC
  841 TTNANANCNT TCTGCAATGN NCTCNAAATT TCGGGTTGNA ANCTCCTTGA NNNNNATGNN
  901 TTTNCNAACC ACCCNNCNTT TTTGNNNCNN GNCTCCATNN NCCNNNNCCN NGGGNNNNNN
  961 NGCTNNGGNN TTCTNNNNNG NNNNNCNGNN GGGGCCCNGN TCNNNNCGNN NCCNNGNNNN
1021 NACNNNNNGG NNNNNNCCNN NNTNGGGCNN NNCNTNNNNN NNNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: NeoR_KanR
Sequence file: RDB17728_B0Jud.seq check
>D06721A7_B0Ju_3-3_SV40pro_ori_F_C07_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NCNNCTCGGC CTCTGNNCTN NTCNNGAAGT AGTGAGGAGG
   61 CTTTTTTGGA GGNNNNNNNN NTTGCANNGA TCATCAAGAG ACAGGATGAG GATCGTTTCG
  121 CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT
  181 CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC
  241 AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT
  301 GCAAGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT
  361 GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA
  421 GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT
  481 GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG
  541 CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA
  601 AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGA GCATGCCCGA
  661 CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA
  721 TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGNNGGANC GCTATCANGA
  781 CATANCGNNG GCTACCCGTG ATATTGCTGA ANGANCTTGG CGGNGAATGG GCTGACCGCT
  841 TCNNCGTGCT TTACGGTATC GCCGCTCCCN ANTNNGCAGC GCATCNCCTN NTATCGCNTT
  901 CTTGANNAGT TCTNNTGANC NGGNNNTCTG GGGGTTCNAA ATGNCNGANN NAGNGACNNC
  961 CANNCTGNNN TCACGAGNNT TCNANTNCNC CNNCNNCNTT CTATGAAANN NNNGNNNTNN
1021 NGNNNNNNNT TTNNNNGNNN NNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kashiwagi, K., Structural basis for eIF2B inhibition in integrated stress response. Science 364 (6439): 495-499 (2019). PMID 31048492. [link to RRC of NBRP]