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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCOLA-3C-eIF2Be-eIF2Bg (#RDB17724)

Bacterial expression vector of human eukaryotic translation initiation factor 2B subunit gamma (eIF2Bgamma) and human eukaryotic translation initiation factor 2B subunit epsilon (eIF2Bepsilon).

Alternative name eIF2Bgamma-epsilon
Clone info. Bacterial expression vector of human eukaryotic translation initiation factor 2B subunit gamma (eIF2Bgamma) and human eukaryotic translation initiation factor 2B subunit epsilon (eIF2Bepsilon) cloned into the pCOLADuet-1. An HRV 3C protease cleavable His6-tag was added to the N-terminus of eIF2Bepsilon.
Comment Expression was confirmed by the depositor.
Vector backbone pCOLADuet-1 (plasmid)
Size of vector backbone 3.7 kb
Selectable markers Kanamycin
Gene/insert name human EIF2B3 cDNA human EIF2B5 cDNA
Depositor|Developer Ito, Takuhiro |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kashiwagi, K. et al. Science, 364(6439), 495-499, 2019).
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kashiwagi, K. et al. Science, 364(6439), 495-499, 2019)。

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RDB17724 pCOLA-3C-eIF2Be-eIF2Bg DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCOLA-3C-eIF2Be-eIF2Bg was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17724).

Reference section:

Kashiwagi, K., Yokoyama, T., Nishimoto, M., Takahashi, M., Sakamoto, A., Yonemochi, M., Shirouzu, M., Ito, T., Structural basis for eIF2B inhibition in integrated stress response. Science 364 (6439): 495-499 (2019). PMID 31048492. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17724_B0Adp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: lacI-N_R2 (Pr0906)
Region: lacI pro, T7 pro-1, lac OP, RBS, 6xHis, insert-1 5'
Sequence file: RDB17724_B0Ada.seq check
>D06721A3_B0Ad_3_lacI-N_R2_A01_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG GCATACTCTG CGACNTCNNA
   61 TAANCGTTAC TGGTTTCNCA TTCNCCACCC TGAATTGACT CTCTTCCGGG CGCTATCATG
  121 CCATACCGCG AAAGGTTTTG CGCCATTCGA TGGTGTCCGG GATCTCGACG CTCTCCCTTA
  181 TGCGACTCCT GCATTAGGAA ATTAATACGA CTCACTATAG GGGAATTGTG AGCGGATAAC
  241 AATTCCCCTG TAGAAATAAT TTTGTTTAAC TTTAATAAGG AGATATACCA TGGGCAGCAG
  301 CCATCACCAT CATCACCACA GCCAGGATCC GAATTCGAGC TCGGCGCGCC TGCAGGTCGA
  361 CGCGGCCCCT GTAGTGGCGC CGCCTGGTGT GGTGGTTAGT CGGGCTAACA AGCGCAGCGG
  421 CGCGGGGCCG GGAGGCAGCG GTGGCGGGGG AGCCAGAGGG GCGGAGGAGG AACCGCCGCC
  481 GCCCCTACAA GCAGTTCTGG TGGCCGATAG CTTCGATCGC CGCTTCTTCC CCATCTCCAA
  541 GGACCAGCCT CGGGTCCTCT TGCCCCTGNN NNNNGTGGCA TTAATTGACT ACACTCTGGA
  601 ATTCCTGACT GCCACAGGTG NNNNNGAAAC ATTTGTCTTT TGTTGCTGGA AAGCTGCTCA
  661 AATCAAAGAA CATTTACTGA AGTCAAAGTG GTGCCGCCCT ACNTCTCTCN NTGTGGTTCG
  721 AATAATTACA TCANANCTCT ATCNNNTCAC TGGGAGATGT CCTCCGNGAT GTTGATGCCN
  781 ANGGCTTTTG GNGCGCTCTG ANTTTCTTCT GGNGTATGGG GATGTCATCT CNANNNNNNN
  841 AATATCACCA NANCCCTTNG ANGAANNNAN GTTNNANANG GAANNNTNNN AAAAAANNGT
  901 TTTCTGTGAT GACNATGANC NTTNNAGGAN TNNNNNNNCN ANNCNNNCCN NNTNNNNTNN
961 NNNCNNANNA NAANNGNNGN TANNNNNNNN NN
//
Primer: hEIF2B3_R (Pr0904)
Region: insert-2 5', lac OP, T7pro-2, insert-1 3'
Sequence file: RDB17724_B0Adb.seq check
>D06721A3_B0Ad_3_hEIF2B3_R_A02_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN TNNNNNNNGG NANCAGANGT TTGGGAATGC
   61 TGGAAGTTAG GTCTGTCATC CGAGNNCNNC CACCTACTGC CATCACTACT GCTTGAAATT
  121 CCATATGTAT ATCTCCTTCT TATACTTAAC TAATATACTA AGATGGGGAA TTGTTATCCG
  181 CTCACAATTC CCCTATAGTG AGTCGTATTA ATTTCGATTA TGCGGCCGTG TACAATACGA
  241 TTACTTTCTG TTCGACTTAA GCATTATGCG GCCGCTCAGT CATCTTCAGA TGACTCCTCT
  301 TCTGCCTCTT TTAGCCACTG GATGAACCTC TGCAGCTGTT GATTCTTGCG CAACTGCTGG
  361 CCCTTGTCAG TTGTATCTCT TTGGCTGAAC CAGCTCAGAA TTGTTTCCTC AGCCAGGATC
  421 TCCAGCTGGT AGAAAGCCAT CAGTACCTTG GCCATGGAAA TACCAAGAGC TTCATGCTCT
  481 AGGAAGAAGT CCTCAATGGC TGCTAACGCT TCCAAATGGT CGGCTGCGCG CTTTATGTAG
  541 TTCCTAAAAA CAGGGCTCCA GGCCTTTAGC AGAGGAAGCA GCAGGGCACA GTAGCGGCTT
  601 GAGTCAAGCG GGGAATCCAT CTGTTGCAGG GGGAACTCCA GGACCACGTG GCTCAGTACC
  661 TGCATCACCT CCTTTAGACT TATGTTATAG GCATACTTGA GAGAGTTGAT TTCCAGGACG
  721 AGATTGTCAC AAGAAATGTT CTCCTCTTTG CCCCGCTGTA NTGTTCCTAA AACTTCATTC
  781 TGGAACACTT TGATGTCATC CATCTGANGG GAGCCTCCCC GGCTGTCCGG CTCCTCANAA
  841 TCCATACTTT GCTCACTTTC ACTTTCACTC TCTNCTTCCN TGTTGATCTT GAGTCCCCNC
  901 AGANTCTGCT GCANTTCNNN CTCNTCCNCC ANGTTCATGC CTGCANCTTT CNNNANGTAG
  961 NCNTTGNCAN CAGCTCCTAC TTNCTGCTGN ANNGNANNNN NTNNNNTNCN CNTTNGNCCN
1021 TTTNCTNNGN NNNNNCNNNN NNNNNTNNNN NNANNTCNNN NNNNNNN
//
Primer: T7termi3'_R (Pr0900)
Region: T7 ter, insert-2 3'
Sequence file: RDB17724_B0Adc.seq check
>D06721A3_B0Ad_1_T7Termi3'_R_D04_10_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN CTGNNNNNNC GGNTNNTTGA CNACCGGAAG
   61 CAGTGTGNNC GTGTGCTTCT CNNATGCCTG AGGTTTCAGC AAAAAACCCC TCAAGACCCG
  121 TTTAGAGGCC CCAAGGGGTT ATGCTAGTTA TTGCTCAGCG GTGGCAGCAG CCTAGGTTAA
  181 TTAAGCTGCG CTAGTAGACG AGTCCATGTG CTGGCGTTCA AATTTCGCAG CAGCGGTTTC
  241 TTTACCAGAC TCGAGGGTAC CGACGTCTCA GATCTCCATG AGCTGGTCAT TCCCCACGAT
  301 CACCTCATTC ACTCGTTTAG CTTTGGCTTC AATCCTCTGG CCACTTCCAA TCAAGCAGTC
  361 CTTGATGTCT GCACCCTTCT CGATCACAGC ATTGTTGCAG ATGACACTGC CTTGGATATT
  421 GCTTCCTTCC TCCACAGTGA CTGAGTTCAT GAGAAGGCAA TTGGTAATAG TCACTCTATC
  481 TTTTATGAGA CAGGATGAGC CAATGACTGA GCGCTTAATG GATGACTTCT CTCCAATCTG
  541 TGTCTCTGGC CCAATGAGGC TGTCAACTCC AACCAGGTGT TTGCTGACAA TCTGGGCTGA
  601 CGAATGGACT GGTGGTTCTT CTGGACAGAG AGCAGACAGC AATTTGGGCA CCTGTCTGTT
  661 TGCTTCCATG TAGAGTCCCA NNGNGCTCAC TCGAGAGCAG AGCCCCTCTT TCATGATGTG
  721 GACATAGCAG CGCACCTGTG ATCTGGACAA GTCTTCCCAC CTGTCTCCTC GACAGGCATT
  781 CCAGCAGGCA TCATANGGGA GCCAGGNTCA GTGTATTGGN CTTCTTTTTA TAAANNTGTA
  841 GATATCNNNN GNNTTCANCN NCNTTTTNNT TTAGATCCNC CTCTTTTTCT NNTNGNNCCC
  901 TGTTGNGAGG AANCTGANGA AANNTGTTTT CTCACTAAAT ATGGNNNNNN NNCACTNCNN
  961 GATAGANNNN TANTNNANCN CNTTTTNNNN NTNNGNNAAN CCNNNGATNN NTTTTTTTCN
1021 AANNNNNNNN NN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kashiwagi, K., Structural basis for eIF2B inhibition in integrated stress response. Science 364 (6439): 495-499 (2019). PMID 31048492. [link to RRC of NBRP]