Please review the QC test results indicated by icon below as well as clone information before placing your order.

pCOLA-3C-eIF2Be-eIF2Bg

Bacterial expression vector of human eukaryotic translation initiation factor 2B subunit gamma (eIF2Bgamma) and human eukaryotic translation initiation factor 2B subunit epsilon (eIF2Bepsilon).

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kashiwagi, K. et al. Science, 364(6439), 495-499, 2019).
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kashiwagi, K. et al. Science, 364(6439), 495-499, 2019)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB17724_B0Adp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: lacI-N_R2 Region: lacI pro, T7 pro-1, lac OP, RBS, 6xHis, insert-1 5' Sequence file: RDB17724_B0Ada.seq
>D06721A3_B0Ad_3_lacI-N_R2_A01_01_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG GCATACTCTG CGACNTCNNA    61 TAANCGTTAC TGGTTTCNCA TTCNCCACCC TGAATTGACT CTCTTCCGGG CGCTATCATG   121 CCATACCGCG AAAGGTTTTG CGCCATTCGA TGGTGTCCGG GATCTCGACG CTCTCCCTTA   181 TGCGACTCCT GCATTAGGAA ATTAATACGA CTCACTATAG GGGAATTGTG AGCGGATAAC   241 AATTCCCCTG TAGAAATAAT TTTGTTTAAC TTTAATAAGG AGATATACCA TGGGCAGCAG   301 CCATCACCAT CATCACCACA GCCAGGATCC GAATTCGAGC TCGGCGCGCC TGCAGGTCGA   361 CGCGGCCCCT GTAGTGGCGC CGCCTGGTGT GGTGGTTAGT CGGGCTAACA AGCGCAGCGG   421 CGCGGGGCCG GGAGGCAGCG GTGGCGGGGG AGCCAGAGGG GCGGAGGAGG AACCGCCGCC   481 GCCCCTACAA GCAGTTCTGG TGGCCGATAG CTTCGATCGC CGCTTCTTCC CCATCTCCAA   541 GGACCAGCCT CGGGTCCTCT TGCCCCTGNN NNNNGTGGCA TTAATTGACT ACACTCTGGA   601 ATTCCTGACT GCCACAGGTG NNNNNGAAAC ATTTGTCTTT TGTTGCTGGA AAGCTGCTCA   661 AATCAAAGAA CATTTACTGA AGTCAAAGTG GTGCCGCCCT ACNTCTCTCN NTGTGGTTCG   721 AATAATTACA TCANANCTCT ATCNNNTCAC TGGGAGATGT CCTCCGNGAT GTTGATGCCN   781 ANGGCTTTTG GNGCGCTCTG ANTTTCTTCT GGNGTATGGG GATGTCATCT CNANNNNNNN   841 AATATCACCA NANCCCTTNG ANGAANNNAN GTTNNANANG GAANNNTNNN AAAAAANNGT   901 TTTCTGTGAT GACNATGANC NTTNNAGGAN TNNNNNNNCN ANNCNNNCCN NNTNNNNTNN 961 NNNCNNANNA NAANNGNNGN TANNNNNNNN NN //
Primer: hEIF2B3_R Region: insert-2 5', lac OP, T7pro-2, insert-1 3' Sequence file: RDB17724_B0Adb.seq
>D06721A3_B0Ad_3_hEIF2B3_R_A02_01_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN TNNNNNNNGG NANCAGANGT TTGGGAATGC    61 TGGAAGTTAG GTCTGTCATC CGAGNNCNNC CACCTACTGC CATCACTACT GCTTGAAATT   121 CCATATGTAT ATCTCCTTCT TATACTTAAC TAATATACTA AGATGGGGAA TTGTTATCCG   181 CTCACAATTC CCCTATAGTG AGTCGTATTA ATTTCGATTA TGCGGCCGTG TACAATACGA   241 TTACTTTCTG TTCGACTTAA GCATTATGCG GCCGCTCAGT CATCTTCAGA TGACTCCTCT   301 TCTGCCTCTT TTAGCCACTG GATGAACCTC TGCAGCTGTT GATTCTTGCG CAACTGCTGG   361 CCCTTGTCAG TTGTATCTCT TTGGCTGAAC CAGCTCAGAA TTGTTTCCTC AGCCAGGATC   421 TCCAGCTGGT AGAAAGCCAT CAGTACCTTG GCCATGGAAA TACCAAGAGC TTCATGCTCT   481 AGGAAGAAGT CCTCAATGGC TGCTAACGCT TCCAAATGGT CGGCTGCGCG CTTTATGTAG   541 TTCCTAAAAA CAGGGCTCCA GGCCTTTAGC AGAGGAAGCA GCAGGGCACA GTAGCGGCTT   601 GAGTCAAGCG GGGAATCCAT CTGTTGCAGG GGGAACTCCA GGACCACGTG GCTCAGTACC   661 TGCATCACCT CCTTTAGACT TATGTTATAG GCATACTTGA GAGAGTTGAT TTCCAGGACG   721 AGATTGTCAC AAGAAATGTT CTCCTCTTTG CCCCGCTGTA NTGTTCCTAA AACTTCATTC   781 TGGAACACTT TGATGTCATC CATCTGANGG GAGCCTCCCC GGCTGTCCGG CTCCTCANAA   841 TCCATACTTT GCTCACTTTC ACTTTCACTC TCTNCTTCCN TGTTGATCTT GAGTCCCCNC   901 AGANTCTGCT GCANTTCNNN CTCNTCCNCC ANGTTCATGC CTGCANCTTT CNNNANGTAG   961 NCNTTGNCAN CAGCTCCTAC TTNCTGCTGN ANNGNANNNN NTNNNNTNCN CNTTNGNCCN 1021 TTTNCTNNGN NNNNNCNNNN NNNNNTNNNN NNANNTCNNN NNNNNNN //
Primer: T7termi3'_R Region: T7 ter, insert-2 3' Sequence file: RDB17724_B0Adc.seq
>D06721A3_B0Ad_1_T7Termi3'_R_D04_10_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN CTGNNNNNNC GGNTNNTTGA CNACCGGAAG    61 CAGTGTGNNC GTGTGCTTCT CNNATGCCTG AGGTTTCAGC AAAAAACCCC TCAAGACCCG   121 TTTAGAGGCC CCAAGGGGTT ATGCTAGTTA TTGCTCAGCG GTGGCAGCAG CCTAGGTTAA   181 TTAAGCTGCG CTAGTAGACG AGTCCATGTG CTGGCGTTCA AATTTCGCAG CAGCGGTTTC   241 TTTACCAGAC TCGAGGGTAC CGACGTCTCA GATCTCCATG AGCTGGTCAT TCCCCACGAT   301 CACCTCATTC ACTCGTTTAG CTTTGGCTTC AATCCTCTGG CCACTTCCAA TCAAGCAGTC   361 CTTGATGTCT GCACCCTTCT CGATCACAGC ATTGTTGCAG ATGACACTGC CTTGGATATT   421 GCTTCCTTCC TCCACAGTGA CTGAGTTCAT GAGAAGGCAA TTGGTAATAG TCACTCTATC   481 TTTTATGAGA CAGGATGAGC CAATGACTGA GCGCTTAATG GATGACTTCT CTCCAATCTG   541 TGTCTCTGGC CCAATGAGGC TGTCAACTCC AACCAGGTGT TTGCTGACAA TCTGGGCTGA   601 CGAATGGACT GGTGGTTCTT CTGGACAGAG AGCAGACAGC AATTTGGGCA CCTGTCTGTT   661 TGCTTCCATG TAGAGTCCCA NNGNGCTCAC TCGAGAGCAG AGCCCCTCTT TCATGATGTG   721 GACATAGCAG CGCACCTGTG ATCTGGACAA GTCTTCCCAC CTGTCTCCTC GACAGGCATT   781 CCAGCAGGCA TCATANGGGA GCCAGGNTCA GTGTATTGGN CTTCTTTTTA TAAANNTGTA   841 GATATCNNNN GNNTTCANCN NCNTTTTNNT TTAGATCCNC CTCTTTTTCT NNTNGNNCCC   901 TGTTGNGAGG AANCTGANGA AANNTGTTTT CTCACTAAAT ATGGNNNNNN NNCACTNCNN   961 GATAGANNNN TANTNNANCN CNTTTTNNNN NTNNGNNAAN CCNNNGATNN NTTTTTTTCN 1021 AANNNNNNNN NN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl