Bacterial expression vector of human eukaryotic translation initiation factor 2B subunit gamma (eIF2Bgamma) and human eukaryotic translation initiation factor 2B subunit epsilon (eIF2Bepsilon).
Alternative name | eIF2Bgamma-epsilon |
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Clone info. | Bacterial expression vector of human eukaryotic translation initiation factor 2B subunit gamma (eIF2Bgamma) and human eukaryotic translation initiation factor 2B subunit epsilon (eIF2Bepsilon) cloned into the pCOLADuet-1. An HRV 3C protease cleavable His6-tag was added to the N-terminus of eIF2Bepsilon. |
Comment | Expression was confirmed by the depositor. |
Vector backbone | pCOLADuet-1 (plasmid) |
Size of vector backbone | 3.7 kb |
Selectable markers | Kanamycin |
Gene/insert name | human EIF2B3 cDNA human EIF2B5 cDNA |
Depositor|Developer | Ito, Takuhiro | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kashiwagi, K. et al. Science, 364(6439), 495-499, 2019). |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kashiwagi, K. et al. Science, 364(6439), 495-499, 2019)。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB17724 | pCOLA-3C-eIF2Be-eIF2Bg | DNA solution |
Materials & Methods section:
The pCOLA-3C-eIF2Be-eIF2Bg was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17724). |
Reference section:
Kashiwagi, K., Yokoyama, T., Nishimoto, M., Takahashi, M., Sakamoto, A., Yonemochi, M., Shirouzu, M., Ito, T., Structural basis for eIF2B inhibition in integrated stress response. Science 364 (6439): 495-499 (2019). PMID 31048492. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB17724_B0Adp1-1.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: lacI-N_R2 (Pr0906) Region: lacI pro, T7 pro-1, lac OP, RBS, 6xHis, insert-1 5' Sequence file: RDB17724_B0Ada.seq ![]() |
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>D06721A3_B0Ad_3_lacI-N_R2_A01_01_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG GCATACTCTG CGACNTCNNA 61 TAANCGTTAC TGGTTTCNCA TTCNCCACCC TGAATTGACT CTCTTCCGGG CGCTATCATG 121 CCATACCGCG AAAGGTTTTG CGCCATTCGA TGGTGTCCGG GATCTCGACG CTCTCCCTTA 181 TGCGACTCCT GCATTAGGAA ATTAATACGA CTCACTATAG GGGAATTGTG AGCGGATAAC 241 AATTCCCCTG TAGAAATAAT TTTGTTTAAC TTTAATAAGG AGATATACCA TGGGCAGCAG 301 CCATCACCAT CATCACCACA GCCAGGATCC GAATTCGAGC TCGGCGCGCC TGCAGGTCGA 361 CGCGGCCCCT GTAGTGGCGC CGCCTGGTGT GGTGGTTAGT CGGGCTAACA AGCGCAGCGG 421 CGCGGGGCCG GGAGGCAGCG GTGGCGGGGG AGCCAGAGGG GCGGAGGAGG AACCGCCGCC 481 GCCCCTACAA GCAGTTCTGG TGGCCGATAG CTTCGATCGC CGCTTCTTCC CCATCTCCAA 541 GGACCAGCCT CGGGTCCTCT TGCCCCTGNN NNNNGTGGCA TTAATTGACT ACACTCTGGA 601 ATTCCTGACT GCCACAGGTG NNNNNGAAAC ATTTGTCTTT TGTTGCTGGA AAGCTGCTCA 661 AATCAAAGAA CATTTACTGA AGTCAAAGTG GTGCCGCCCT ACNTCTCTCN NTGTGGTTCG 721 AATAATTACA TCANANCTCT ATCNNNTCAC TGGGAGATGT CCTCCGNGAT GTTGATGCCN 781 ANGGCTTTTG GNGCGCTCTG ANTTTCTTCT GGNGTATGGG GATGTCATCT CNANNNNNNN 841 AATATCACCA NANCCCTTNG ANGAANNNAN GTTNNANANG GAANNNTNNN AAAAAANNGT 901 TTTCTGTGAT GACNATGANC NTTNNAGGAN TNNNNNNNCN ANNCNNNCCN NNTNNNNTNN 961 NNNCNNANNA NAANNGNNGN TANNNNNNNN NN // |
Primer: hEIF2B3_R (Pr0904) Region: insert-2 5', lac OP, T7pro-2, insert-1 3' Sequence file: RDB17724_B0Adb.seq ![]() |
>D06721A3_B0Ad_3_hEIF2B3_R_A02_01_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN TNNNNNNNGG NANCAGANGT TTGGGAATGC 61 TGGAAGTTAG GTCTGTCATC CGAGNNCNNC CACCTACTGC CATCACTACT GCTTGAAATT 121 CCATATGTAT ATCTCCTTCT TATACTTAAC TAATATACTA AGATGGGGAA TTGTTATCCG 181 CTCACAATTC CCCTATAGTG AGTCGTATTA ATTTCGATTA TGCGGCCGTG TACAATACGA 241 TTACTTTCTG TTCGACTTAA GCATTATGCG GCCGCTCAGT CATCTTCAGA TGACTCCTCT 301 TCTGCCTCTT TTAGCCACTG GATGAACCTC TGCAGCTGTT GATTCTTGCG CAACTGCTGG 361 CCCTTGTCAG TTGTATCTCT TTGGCTGAAC CAGCTCAGAA TTGTTTCCTC AGCCAGGATC 421 TCCAGCTGGT AGAAAGCCAT CAGTACCTTG GCCATGGAAA TACCAAGAGC TTCATGCTCT 481 AGGAAGAAGT CCTCAATGGC TGCTAACGCT TCCAAATGGT CGGCTGCGCG CTTTATGTAG 541 TTCCTAAAAA CAGGGCTCCA GGCCTTTAGC AGAGGAAGCA GCAGGGCACA GTAGCGGCTT 601 GAGTCAAGCG GGGAATCCAT CTGTTGCAGG GGGAACTCCA GGACCACGTG GCTCAGTACC 661 TGCATCACCT CCTTTAGACT TATGTTATAG GCATACTTGA GAGAGTTGAT TTCCAGGACG 721 AGATTGTCAC AAGAAATGTT CTCCTCTTTG CCCCGCTGTA NTGTTCCTAA AACTTCATTC 781 TGGAACACTT TGATGTCATC CATCTGANGG GAGCCTCCCC GGCTGTCCGG CTCCTCANAA 841 TCCATACTTT GCTCACTTTC ACTTTCACTC TCTNCTTCCN TGTTGATCTT GAGTCCCCNC 901 AGANTCTGCT GCANTTCNNN CTCNTCCNCC ANGTTCATGC CTGCANCTTT CNNNANGTAG 961 NCNTTGNCAN CAGCTCCTAC TTNCTGCTGN ANNGNANNNN NTNNNNTNCN CNTTNGNCCN 1021 TTTNCTNNGN NNNNNCNNNN NNNNNTNNNN NNANNTCNNN NNNNNNN // |
Primer: T7termi3'_R (Pr0900) Region: T7 ter, insert-2 3' Sequence file: RDB17724_B0Adc.seq ![]() |
>D06721A3_B0Ad_1_T7Termi3'_R_D04_10_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN CTGNNNNNNC GGNTNNTTGA CNACCGGAAG 61 CAGTGTGNNC GTGTGCTTCT CNNATGCCTG AGGTTTCAGC AAAAAACCCC TCAAGACCCG 121 TTTAGAGGCC CCAAGGGGTT ATGCTAGTTA TTGCTCAGCG GTGGCAGCAG CCTAGGTTAA 181 TTAAGCTGCG CTAGTAGACG AGTCCATGTG CTGGCGTTCA AATTTCGCAG CAGCGGTTTC 241 TTTACCAGAC TCGAGGGTAC CGACGTCTCA GATCTCCATG AGCTGGTCAT TCCCCACGAT 301 CACCTCATTC ACTCGTTTAG CTTTGGCTTC AATCCTCTGG CCACTTCCAA TCAAGCAGTC 361 CTTGATGTCT GCACCCTTCT CGATCACAGC ATTGTTGCAG ATGACACTGC CTTGGATATT 421 GCTTCCTTCC TCCACAGTGA CTGAGTTCAT GAGAAGGCAA TTGGTAATAG TCACTCTATC 481 TTTTATGAGA CAGGATGAGC CAATGACTGA GCGCTTAATG GATGACTTCT CTCCAATCTG 541 TGTCTCTGGC CCAATGAGGC TGTCAACTCC AACCAGGTGT TTGCTGACAA TCTGGGCTGA 601 CGAATGGACT GGTGGTTCTT CTGGACAGAG AGCAGACAGC AATTTGGGCA CCTGTCTGTT 661 TGCTTCCATG TAGAGTCCCA NNGNGCTCAC TCGAGAGCAG AGCCCCTCTT TCATGATGTG 721 GACATAGCAG CGCACCTGTG ATCTGGACAA GTCTTCCCAC CTGTCTCCTC GACAGGCATT 781 CCAGCAGGCA TCATANGGGA GCCAGGNTCA GTGTATTGGN CTTCTTTTTA TAAANNTGTA 841 GATATCNNNN GNNTTCANCN NCNTTTTNNT TTAGATCCNC CTCTTTTTCT NNTNGNNCCC 901 TGTTGNGAGG AANCTGANGA AANNTGTTTT CTCACTAAAT ATGGNNNNNN NNCACTNCNN 961 GATAGANNNN TANTNNANCN CNTTTTNNNN NTNNGNNAAN CCNNNGATNN NTTTTTTTCN 1021 AANNNNNNNN NN // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Kashiwagi, K., Structural basis for eIF2B inhibition in integrated stress response. Science 364 (6439): 495-499 (2019). PMID 31048492. [link to RRC of NBRP] |
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