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pET28-3C-eIF2Ba (#RDB17722)

Bacterial expression vector of human eukaryotic translation initiation factor 2B subunit alpha (eIF2Balpha).


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Kashiwagi, K., Science 364 (6439): 495-499 (2019). PMID 31048492. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Alternative name eIF2Balpha
Clone info. Bacterial expression vector of human eukaryotic translation initiation factor 2B subunit alpha (eIF2Balpha) cloned into the pET-28c, in which the thrombin cleavage site was replaced by the HRV 3C protease cleavage site.
Comment Expression was confirmed by the depositor.
Vector backbone pET28c (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Kanamycin
Growth conditions LB+Kanamycin, 37oC
Gene/insert name human EIF2B1 cDNA
Depositor|Developer Ito, Takuhiro |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kashiwagi, K. et al. Science, 364(6439), 495-499, 2019).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kashiwagi, K. et al. Science, 364(6439), 495-499, 2019)。

Catalog # Resource name Shipping form Fee
RDB17722 pET28-3C-eIF2Ba DNA solution

How to cite this biological resource

Materials & Methods section:

The pET28-3C-eIF2Ba was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17722).

Reference section:

Kashiwagi, K., Yokoyama, T., Nishimoto, M., Takahashi, M., Sakamoto, A., Yonemochi, M., Shirouzu, M., Ito, T., Structural basis for eIF2B inhibition in integrated stress response. Science 364 (6439): 495-499 (2019). PMID 31048492. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Kashiwagi, K., Structural basis for eIF2B inhibition in integrated stress response. Science 364 (6439): 495-499 (2019). PMID 31048492. [PubMed] [Article] [RRC of NBRP]