Please review the QC test results indicated by icon below as well as clone information before placing your order.

CSII FLAG-hRFWD3 LQP/AAA

Lentivirus expression vector of human ring finger and WD repeat domain 3 (RFWD3) mutant, LQP/AAA, LQP motifs replaced by triple alanines.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Inano, S et al. Mol. Cell, 66(5): 622-634, 2017)
Additional terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
Ordering	Please visit Ordering instruction.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
Remarks	Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Inano, S et al. Mol. Cell, 66(5): 622-634, 2017)。
付加的提供条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
提供依頼	手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。 提供依頼書 [Word] 提供同意書 (非営利機関による非営利学術研究用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
備考	Gateway®テクノロジーを用いた発現クローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB17501_A9Iop1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: hRFWD3-N_R2 Region: insert 5' (LQP/AAAx3), attB1, FLAG, CMV pro 2, CMV en 2 Sequence file: RDB17501_A9Ioa.seq
>D06551B3_A9Io_1_hRFWD3-N_R2_C01_03_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNGCCN GGGGNGGTGT CGCTTGGCTG CTGATCACCT    61 CAGCAGGAGC TGCAGCTGCG ATGGATGGTA CCCCCTGGCT GCTGACCACA TCAGCAGGAA   121 CAGCTGCAGC GAGGGCTGGT CCCCCTTGGC TGCTGGCCAT GCCAGCAGGA GCTGCAGCTG   181 CTTCGGCATG ATTTAACTGC ACCTGAACAT CATATTCCAT TGCTTCATGA GCCATGGTGA   241 AGGGGGCGGC CGCGGAGCCT GCTTTTTTGT ACAAACTTGT TTGATAGCTT GGACCAGAAC   301 CACCATGCTT ATCGTCATCA TCTTTATAAT CCATGGTGCT AGCCAGCTTG GGTCTCCCTA   361 GCGGATCTGA CGGTTCACTA AACCAGCTCT GCTTATATAG ACCTCCCACC GTACACGCCT   421 ACCGCCCATT TGCGTCAATG GGGCGGAGTT GTTACGACAT TTTGGAAAGT CCCGTTGATT   481 TTGGTGCCAA AACAAACTCC CATTGACGTC AATGGGGTGG AGACTTGGAA ATCCCCGTGA   541 GTCAAACCGC TATCCACGCC CATTGATGTA CTGCCAAAAC CGCATCACCA TGGTAATAGC   601 GATGACTAAT ACGTAGATGT ACTGCCAAGT AGGAAAGTCC CATAAGGTCA TGTACTGGGC   661 ATAATGCCAG GCGGGCCATT TACCGTCATT GACGTCAATA GGGGGCGTAC TTGGCATATG   721 ATACACTTGA TGTACTGCCA AGTGGGCAGT TTACCGTAAA TACTCCACCC ATTGACGTCA   781 ATGGAAAGTC CCTATTGNNG TTACTATGGG AACATACGTC ATTATTGACG TCAATGGGNC   841 GGGGGNCGTN GGGCGGNCAG CCAGGCGGGC CATTTTACNG NAANGTTNAT GTAANNGCGG   901 AACTNNNNNN TATGGGNCTA TGAANNTAAT GNNCCCCCNN NANTTGNNNA CNNNTTNANT   961 CNNANNTNAA NTNCAAAANN GNAATTTNNN NCNNNTCCNN NNNANTNAAN NCCNNNAAAN 1021 NTTTNNNANN TTTNTNNNNN NTTNNNTTTT TNNNNANNNN NNTTNNNNNN NNNN //
Primer: IRES_R3 Region: attB2, inset 3' Sequence file: RDB17501_A9Iob.seq
>D06551B3_A9Io_1_IRES_R3_C02_08_ABI24.ab1     1 NNNNNNNNNN NNNGGGNNGG NNGGNNNNGG GCGGNNCCGC GGCCGCTGCA GAATTCTCGA    61 TGGCAACTAG AAGGCACAGT CGAGGCTGAT CAGCGGGTTT ATCGAACCAC TTTGTACAAG   121 AAAGCTGGGT CGGCGCGCCC ACCCTTTCAC TCCCACTTAT AGATGTGGAC CATCTTCTCT   181 GTTAAGGTAG CCAAGTAGCT GTTACGGTTC ACCTCAAATG GGCAGATGTC CAACACAGGC   241 TGATCGGTCT GTAGGTCCTG GAGCAACGAG CCACTGGCAG CATCCCACAG CAGGGCAGAA   301 TTTGCTGCTT CATCCCCAGT ACACACCAGG ATGTTGCCAT CATTCTCTGG GCTTTGGAAA   361 ATGGCATTTT TGGTCAATAG TTTGCAAGTA GGTCCTCCAA AAAATGTATG TACAGGCTGG   421 CAGGAGCAGA TTGGATTTCC AGTGTCATCC AGTCGGTAGG ACATTTCCAT CAGCACACTT   481 CGTATGGTGG TGTGATTTTT ATCAGGCCTG TAGGTCACAA GACAGTGCCG GGAGCTGTTC   541 TCTGTCTGAA AGTCTATGCA GCCCCCTGGC TCCAAGGGCA GCACATGAGG CCAATGAGAA   601 AAGTCCATTT TCTGTTCCCA GAATGAAGCA TCCTCCAAGG TTCCAGCCAG CACCCCACCA   661 TATGGAAATG CAGCTGAGGC AGCTCTGGGC ATGTATGACA GGGAGACCAG TGGGCATCTG   721 GCTTTCTGAG CTACTAACTC CTGCACATGA CTGCTCGTGT TTCGCACGTC ATATACCAGA   781 ATTGAACCAT TGGCCAGTCC AGCATAGATG TAGTTAGCCT CATCAAGACA CCAGCAACAG   841 CTCCAGACAG GACGTCCAGC ATTATAAGTC TGGACCACGG NATTTGTCTN CAGGCTGGTC   901 AGTTTAATAG TGTTGTCTAN GGAANCAGAN AGTANCAAGC NNCTNANGNN ANTGCTAAAC   961 GCCAGTCCNC NGNTCTGNTT GNNNTGCNTN NNNNNGTNNT GNNNGNNNTT CNNGNTNNNN 1021 NNNNNN //
Primer: CSII_CMV5_v2 Region: FLAG, attB1, insert 5' (LQP/AAAx4) Sequence file: RDB17501_A9Ioc.seq
>D06551B3_A9Io_1_CSII_CMV5_v2_B02_05_ABI24.ab1     1 NNNNNNNNNN NNNNGCNGGC NNGCNCCNTG GNNTATAAAG ANGANGACGA TAAGCATGGT    61 GGTTCTGGTC CAAGCTATCA AACAAGTTTG TACAAAAAAG CAGGCTCCGC GGCCGCCCCC   121 TTCACCATGG CTCATGAAGC AATGGAATAT GATGTTCAGG TGCAGTTAAA TCATGCCGAA   181 GCAGCTGCAG CTCCTGCTGG CATGGCCAGC AGCCAAGGGG GACCAGCCCT CGCTGCAGCT   241 GTTCCTGCTG ATGTGGTCAG CAGCCAGGGG GTACCATCCA TCGCAGCTGC AGCTCCTGCT   301 GAGGTGATCA GCAGCCAAGC GACACCACCC CTGGCAGCAG CTGCTCCGCA ACTGTCTGTT   361 GACCTGACAG AAGTGGAGGT CTTGGGAGAA GACACTGTGG AGAACATCAA TCCAAGAACT   421 TCAGAACAAC ATAGGCAGGG ATCTGATGGT AATCACACCA TCCCAGCATC TTCGTTGCAT   481 TCAATGACCA ACTTCATCAG CGGACTGCAG AGACTTCATG GCATGCTGGA ATTCCTGAGA   541 CCTTCATCTT CAAACCACAG TGTAGGGCCA ATGAGAACAA GAAGGAGGGT ATCTGCTTCA   601 CGGAGGGCAA GAGCCGGAGG GTCTCAGAGG ACAGACAGTG CCAGGTTGAG AGCACCATTG   661 GATGCTTACT TTCAGGTGAG CAGGACCCAG CCTGACTTGC CAGCTACCAC TTATGATTCA   721 GAGACTAGGA ATCCTGTATC TGAAGAGTTG CAGGTGTCTA GTAGTTCTGA TTCTGACAGT   781 GACAGCTCTG CAGAGTATGG ANGGGTTGTT GANCAGGCAG ANGAATCTGG ANCTGTCATT   841 TTAGAAGANC AACTAGCANG TGTCTCANCA NANCAAGAAG TTACATGTAT CGATGNNNGC   901 AAGANCNTCC NNNAANAGCN NTCTCCCNNG AANNCTGANC NNNNGCTNCC TTCNGCTTNT 961 NNNGNTNNNN NNNNNN //
Primer: HIVgp_R1 Region: HIV-1 psi, , 5' LTR Sequence file: RDB17501_A9Iod.seq
>D06551B3_A9Io_1_HIVgp_R1_D02_11_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNC TCTTTTTTCT CTCTGCACCN CTCTTCTCTT TGCCTTGGTG    61 GGTGCTACTC CTAATGGTTC AATTTTTACT ACTTTATATT TATATAATTC ACTTCTCCAA   121 TTGTCCCTCA TATCTCCTCC TCCAGGTCTG AAGATCAGCG GCCGGCCGCT TGCTGTGCGG   181 TGGTCTTACT TTTGTTTTGC TCTTCCTCTA TCTTGTCTAA AGCTTCCTTG GTGTCTTTTA   241 TCTCTATCCT TTGATGCACA CAATAGAGGG TTGCTACTGT ATTATATAAT GATCTAAGTT   301 CTTCTGATCC TGTCTGAAGG GATGGTTGTA GCTGTCCCAG TATTTGTCTA CAGCCTTCTG   361 ATGTTTCTAA CAGGCCAGGA TTAACTGCGA ATCGTTCTAG CTCCCTGCTT GCCCATACTA   421 TATGTTTTAA TTTATATTTT TTCTTTCCCC CTGGCCTTAA CCGAATTTTT TCCCATCGCG   481 ATCTAATTCT CCCCCGCTTA ATACTGACGC TCTCGCACCC ATCTCTCTCC TTCTAGCCTC   541 CGCTAGTCAA AATTTTTGGC GTACTCACCA GTCGCCGCCC CTCGCCTCTT GCCGTGCGCG   601 CTTCAGCAAG CCGAGTCCTG CGTCGAGAGA GCTCCTCTGG TTTCCCTTTC GCTTTCAAGT   661 CCCTGTTCGG GCGCCACTGC TAGAGATTTT CCACACTGAC TAAAAGGGTC TGAGGGATCT   721 CTAGTTACCA GAGTCACACA ACAGACGGGC ACACACTACT TGAAGCACTC AAGGCAAGCT   781 TTATTGAGGC TTAAGCAGTG GGTTCCCTAN TTAGCCAGAG AGCTCCCAGG CTCAGATCTG   841 GTCTAACCAG AGAGACCCAG TACAGGCAAA ACGCGCTGCT TATATAGACC TCCNACNGNA   901 CACGCNNANC GCCCATTTGC GTCAANGGGG GCGGANTTGT TNCGACATTT TGGAAAGTCC   961 CNNTGATTTT GGNGCNNAAA CNAANTCCCN TTNNNGNNNN NNGGGGNNGN ANNNTNNNNN 1021 ATCCNNNNNN NNNN //
Primer: cPPT+CTS_v2_F Region: cPPT_CTS, CMV en 2, CMV pro 2 Sequence file: RDB17501_A9Ioe.seq
>D06551B3_A9Io_1_cPPT+CTS_v2_F_E02_14_ABI24.ab1     1 NNNNNNNNNN NNNCNNNNNN NNTACNAAAA CNAATTACAA AAATTCAAAA TTTTCGGGTT    61 TATTACAGGG ACAGCAGAAA TTCACTTTGA ATTAATTCGA TTAATAGTAA TCAATTACGG   121 GGTCATTAGT TCATAGCCCA TATATGGAGT TCCGCGTTAC ATAACTTACG GTAAATGGCC   181 CGCCTGGCTG ACCGCCCAAC GACCCCCGCC CATTGACGTC AATAATGACG TATGTTCCCA   241 TAGTAACGCC AATAGGGACT TTCCATTGAC GTCAATGGGT GGAGTATTTA CGGTAAACTG   301 CCCACTTGGC AGTACATCAA GTGTATCATA TGCCAAGTAC GCCCCCTATT GACGTCAATG   361 ACGGTAAATG GCCCGCCTGG CATTATGCCC AGTACATGAC CTTATGGGAC TTTCCTACTT   421 GGCAGTACAT CTACGTATTA GTCATCGCTA TTACCATGGT GATGCGGTTT TGGCAGTACA   481 TCAATGGGCG TGGATAGCGG TTTGACTCAC GGGGATTTCC AAGTCTCCAC CCCATTGACG   541 TCAATGGGAG TTTGTTTTGG CACCAAAATC AACGGGACTT TCCAAAATGT CGTAACAACT   601 CCGCCCCATT GACGCAAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT ATAAGCAGAG   661 CTGGTTTAGT GAACCGNNNN ATCCGCTAGG GAGACCCAAG CTGGCTAGCA CCATGGANTA   721 TAAAGATGAT GACGATAAGC ATGGTGGTTC TGGTCCAAGC TATNNAACAA GTTTGTACNA   781 AAAAGCAGGC TCCGCGGCCG CCCCCTTCAC CATGGCTCAT GAANCAATGN AATATGATGT   841 TCAGGTGCNG TTNAATCATG CCGAANCAGC TGCAGCTNCT GCTGGCATGG CCNGCAGNCA   901 AGGGGNANCA NCCCNCGCTG CANCTGTNCC TGCTNATGTG GNCAGCANCC ANGGGNNNNC 961 ATNCATCNNA GCTGCNGCTN CNNGCTNNNN NGNTCAGCAN NCNNANCNNN NNNNNNN //
Primer: BSD_R1 Region: BSD, IRES2 Sequence file: RDB17501_A9Iof.seq
>D06551B3_A9Io_1_BSD_R1_G02_20_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNCCNN NNNNNGNNGC GGCCGTCGCT NGAGANAGCT    61 GCGCTGGCGA CGCTGTAGTC TTCNNAGATG GGGATGCTGT TGATTGTAGC CGTTGCTCTT   121 TCAATGAGGG TGGATTCTTC TTGAGACAAA GGCTTGGCCA TGGTTGTGGC CATATTATCA   181 TCGTGTTTTT CAAAGGAAAA CCACGTCCCC GTGGTTCGGG GGGCCTAGAC GTTTTTTTAA   241 CCTCGACTAA ACACATGTAA AGCATGTGTA CCGAGGCCCC AGATCAGATC CCATACAATG   301 GGGTACCTTC TGGGCATCCT TCAGCCCCTT GTTGAATACG CTTGAGGAGA GCCATTTGAC   361 TCTTTCCACA ACTATCCAAC TCACAACGTG GCACTGGGGT TGTGCCGCCT TTGCAGGTGT   421 ATCTTATACA CGTGGCTTTT GGCCGCAGAG GCACCTGTCG CCAGGTGGGG GGTTCCGCTG   481 CCTGCAAAGG GTCGCTACAG ACGTTGTTTG TCTTCAAGAA GCTTCCAGAG GAACTGCTTC   541 CTTCACGACA TTCAACAGAC CTTGCATTCC TTTGGCGAGA GGGGAAAGAC CCCTAGGAAT   601 GCTCGTCAAG AAGACAGGGC CAGGTTTCCG GGCCCTCACA TTGCCAAAAG ACGGCAATAT   661 GGTGGAAAAT AACATATAGA CAAACGCACA CCGGCCTTAT TCCAAGCGGC TTCGGCCAGT   721 AACGTTAGGG GGGGGGGAGG GAGAGGGGCG GATCCGCGGC CGCTGCAGAA TTCTCGATGG   781 GCAANTANAA NGGNNNNGTC GANGCTGATC AGCGGGNNTT ATCNAAACCA CTTNNNACNN   841 GNNAANNCTN GNNCGGCGCN NNNCACCCNT TNCNCNNNCN CNTNTANNNG NGNGANNCNT 901 CTTCNCTGNN NANGGNANNC AANNNNNNGN NNNNNNNTCA NCTCNNATGN NNNNNNNNN //
Primer: BGH_rev2 Region: bGH p(A), 3' LTR, WPRE Sequence file: RDB17501_A9Iog.seq
>D06551B3_A9Io_1_BGH_rev2_F02_17_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNGGGGGNNG GGCANCAACA GATGGCTGGC AACTAGAAGG    61 CACAGTCGAG GCTGATCAGC GGGTTTAAAC GGGCCCTGCT AGAGATTTTC CACACTGACT   121 AAAAGGGTCT GAGGGATCTC TAGTTACCAG AGTCACACAA CAGACGGGCA CACACTACTT   181 GAAGCACTCA AGGCAAGCTT TATTGAGGCT TAAGCAGTGG GTTCCCTAGT TAGCCAGAGA   241 GCTCCCAGGC TCAGATCTGG TCTAACCAGA GAGACCCAGT ACAGTCCGGA TGCAGCTCTC   301 GGGCCATGTG ATGAAATGCT AGGCGGCTGT CAAACCTCCA CTCTAATACT TCTCTCTCCG   361 GGTCATCCAT CCCATGCAGG CTCACAGGGT GTAACAAGCG GGTGTTCTCT CCTTCATTGG   421 CTTCTTCTAC CTTCTCTTGC TCAACTGGTA CTAGCTTGTA GCACCATCCA AAGGTCAGTG   481 GATATCTGAT CCCTGGCCCT GGTGTGTAGT TCTGCCAATC AGGGAAGTAG CCTTGTGTGT   541 GGTAGATCCA CAGATCAAGG ATATCTTGTC TTCGTTGGGA GTGAATTAGC CCTTCCAGTC   601 CCCCCTTTTC TTTTAAAAAG TGGCTAAGAT CTACAGCTGC CTTGTAAGTC ATTGGTCTTA   661 AAGGTACCTG AGGTGTGACT GGAAAACCCA CCTCCTCCTC CTCTTGTGCT TCTAGCCAGG   721 CACAATCAGC ATTGGTAGCT GCTGTATTGC TACTTGTGAT TGCTCCATGT TTTTCTAGGT   781 CTCGACGGTA TCGATGCGGG GAGGCGGCCC AAAGGGAGNT CCGANTCGTC TGANGGCGAA   841 NGCGAANANG CGGAAGANGC CGCANANNCG GCAGCANGNC GCGGGAANGN ANNNCCGCTG   901 GNNTGANGGN CGAANNGNAN GTANCANAAN NNNGNNCCNC GNANAANTCC NNGTGGNNAC 961 ACAGGNNANC ANNCNNGGAA NNNANGTCNN CNTNCCCNNN NNNNNNN //
Primer: SV40pro_ori_F Region: SV40 pro_ori, EM7 pro, BleoR, SV40 p(A) Sequence file: RDB17501_A9Ioh.seq
>D06551B3_A9Io_1_SV40pro_ori_F_H02_23_ABI24.ab1     1 NNNNNNNNNN NNNNNNGCCT CTGCCTCTGA NCTATTCNNG AAGTAGTGAG GAGGCTTTTT    61 TGGAGGCCTA GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA   121 TCAGCACGTG TTGACAATTA ATCATCGGCA TAGTATATCG GCATAGTATA ATACGACAAG   181 GTGAGGAACT AAACCATGGC CAAGTTGACC AGTGCCGTTC CGGTGCTCAC CGCGCGCGAC   241 GTCGCCGGAG CGGTCGAGTT CTGGACCGAC CGGCTCGGGT TCTCCCGGGA CTTCGTGGAG   301 GACGACTTCG CCGGTGTGGT CCGGGACGAC GTGACCCTGT TCATCAGCGC GGTCCAGGAC   361 CAGGTGGTGC CGGACAACAC CCTGGCCTGG GTGTGGGTGC GCGGCCTGGA CGAGCTGTAC   421 GCCGAGTGGT CGGAGGTCGT GTCCACGAAC TTCCGGGACG CCTCCGGGCC GGCCATGACC   481 GAGATCGGCG AGCAGCCGTG GGGGCGGGAG TTCGCCCTGC GCGACCCGGC CGGCAACTGC   541 GTGCACTTCG TGGCCGAGGA GCAGGACTGA CACGTGCTAC GAGATTTCGA TTCCACCGCC   601 GCCTTCTATG AAAGGTTGGG CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC   661 CAGCGCGGGG ATCTCATGCT GGAGTTCTTC GCCCACCCCA ACTTGTTTAT TGCAGCTTAT   721 AATGNTTACA AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG   781 CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG TATACNGTCN   841 ANCTCTANCT AGAGCTTGGC GTAATCATGG TCATAGCTGT TNCCTGNGTG AAATTGNTAT   901 CCGCNCACNA TTNCACACNA CATACGANCC NGAAGCATAA AGTGTAAAGC NNGGGGGNGC 961 CNNNNGANTG AGCNNANNNN CATTNNNNGC NNNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl