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CSII FLAG-hRFWD3 LQP/AAA (#RDB17501)

Lentivirus expression vector of human ring finger and WD repeat domain 3 (RFWD3) mutant, LQP/AAA, LQP motifs replaced by triple alanines.

Clone info. Lentivirus expression vector of human ring finger and WD repeat domain 3 (RFWD3) mutant, LQP/AAA, LQP motifs replaced by triple alanines, tagged with FLAG at N-terminus, CMV promoter.
Comment Expression was confirmed by the depositor with immunoblotting. Known differences (at least): G to A substitution at 1269nt of NM_018124.4 (R392K; AGG to AAG).
Vector backbone CSII-CMV-MCS-IRES-Bsd (RDB04385) (plasmid)
Selectable markers Lentivirus transfection: Bsd^r. E. coli: Amp^r. Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Growth conditions LB+Ampicillin, 37oC
Gene/insert name Human RFWD3 cDNA
Depositor|Developer Takata, Minoru |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Ordering instruction.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Inano, S et al. Mol. Cell, 66(5): 622-634, 2017)
Additional terms and conditions:
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
Remarks
Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Inano, S et al. Mol. Cell, 66(5): 622-634, 2017)。
付加的使用条件：
本件研究材料は、非営利機関の非営利学術研究に限って提供する。レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
備考
Gateway®テクノロジーを用いた発現クローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17501 CSII FLAG-hRFWD3 LQP/AAA DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge plus licensing fee equivalent for Gateway® expression clone

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The CSII FLAG-hRFWD3 LQP/AAA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17501).

Reference section:

Inano, S., Sato, K., Katsuki, Y., Kobayashi, W., Tanaka, H., Nakajima, K., Nakada, S., Miyoshi, H., Knies, K., Takaori-Kondo, A., Schindler, D., Ishiai, M., Kurumizaka, H., Takata, M., RFWD3-Mediated Ubiquitination Promotes Timely Removal of Both RPA and RAD51 from DNA Damage Sites to Facilitate Homologous Recombination. Mol. Cell 66 (5): 622-634.e8 (2017). PMID 28575658. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17501_A9Iop1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: hRFWD3-N_R2 (Pr0761)
Region: insert 5' (LQP/AAAx3), attB1, FLAG, CMV pro 2, CMV en 2
Sequence file: RDB17501_A9Ioa.seq check
>D06551B3_A9Io_1_hRFWD3-N_R2_C01_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNGCCN GGGGNGGTGT CGCTTGGCTG CTGATCACCT
   61 CAGCAGGAGC TGCAGCTGCG ATGGATGGTA CCCCCTGGCT GCTGACCACA TCAGCAGGAA
  121 CAGCTGCAGC GAGGGCTGGT CCCCCTTGGC TGCTGGCCAT GCCAGCAGGA GCTGCAGCTG
  181 CTTCGGCATG ATTTAACTGC ACCTGAACAT CATATTCCAT TGCTTCATGA GCCATGGTGA
  241 AGGGGGCGGC CGCGGAGCCT GCTTTTTTGT ACAAACTTGT TTGATAGCTT GGACCAGAAC
  301 CACCATGCTT ATCGTCATCA TCTTTATAAT CCATGGTGCT AGCCAGCTTG GGTCTCCCTA
  361 GCGGATCTGA CGGTTCACTA AACCAGCTCT GCTTATATAG ACCTCCCACC GTACACGCCT
  421 ACCGCCCATT TGCGTCAATG GGGCGGAGTT GTTACGACAT TTTGGAAAGT CCCGTTGATT
  481 TTGGTGCCAA AACAAACTCC CATTGACGTC AATGGGGTGG AGACTTGGAA ATCCCCGTGA
  541 GTCAAACCGC TATCCACGCC CATTGATGTA CTGCCAAAAC CGCATCACCA TGGTAATAGC
  601 GATGACTAAT ACGTAGATGT ACTGCCAAGT AGGAAAGTCC CATAAGGTCA TGTACTGGGC
  661 ATAATGCCAG GCGGGCCATT TACCGTCATT GACGTCAATA GGGGGCGTAC TTGGCATATG
  721 ATACACTTGA TGTACTGCCA AGTGGGCAGT TTACCGTAAA TACTCCACCC ATTGACGTCA
  781 ATGGAAAGTC CCTATTGNNG TTACTATGGG AACATACGTC ATTATTGACG TCAATGGGNC
  841 GGGGGNCGTN GGGCGGNCAG CCAGGCGGGC CATTTTACNG NAANGTTNAT GTAANNGCGG
  901 AACTNNNNNN TATGGGNCTA TGAANNTAAT GNNCCCCCNN NANTTGNNNA CNNNTTNANT
  961 CNNANNTNAA NTNCAAAANN GNAATTTNNN NCNNNTCCNN NNNANTNAAN NCCNNNAAAN
1021 NTTTNNNANN TTTNTNNNNN NTTNNNTTTT TNNNNANNNN NNTTNNNNNN NNNN
//
Primer: IRES_R3 (Pr0573)
Region: attB2, inset 3'
Sequence file: RDB17501_A9Iob.seq check
>D06551B3_A9Io_1_IRES_R3_C02_08_ABI24.ab1
    1 NNNNNNNNNN NNNGGGNNGG NNGGNNNNGG GCGGNNCCGC GGCCGCTGCA GAATTCTCGA
   61 TGGCAACTAG AAGGCACAGT CGAGGCTGAT CAGCGGGTTT ATCGAACCAC TTTGTACAAG
  121 AAAGCTGGGT CGGCGCGCCC ACCCTTTCAC TCCCACTTAT AGATGTGGAC CATCTTCTCT
  181 GTTAAGGTAG CCAAGTAGCT GTTACGGTTC ACCTCAAATG GGCAGATGTC CAACACAGGC
  241 TGATCGGTCT GTAGGTCCTG GAGCAACGAG CCACTGGCAG CATCCCACAG CAGGGCAGAA
  301 TTTGCTGCTT CATCCCCAGT ACACACCAGG ATGTTGCCAT CATTCTCTGG GCTTTGGAAA
  361 ATGGCATTTT TGGTCAATAG TTTGCAAGTA GGTCCTCCAA AAAATGTATG TACAGGCTGG
  421 CAGGAGCAGA TTGGATTTCC AGTGTCATCC AGTCGGTAGG ACATTTCCAT CAGCACACTT
  481 CGTATGGTGG TGTGATTTTT ATCAGGCCTG TAGGTCACAA GACAGTGCCG GGAGCTGTTC
  541 TCTGTCTGAA AGTCTATGCA GCCCCCTGGC TCCAAGGGCA GCACATGAGG CCAATGAGAA
  601 AAGTCCATTT TCTGTTCCCA GAATGAAGCA TCCTCCAAGG TTCCAGCCAG CACCCCACCA
  661 TATGGAAATG CAGCTGAGGC AGCTCTGGGC ATGTATGACA GGGAGACCAG TGGGCATCTG
  721 GCTTTCTGAG CTACTAACTC CTGCACATGA CTGCTCGTGT TTCGCACGTC ATATACCAGA
  781 ATTGAACCAT TGGCCAGTCC AGCATAGATG TAGTTAGCCT CATCAAGACA CCAGCAACAG
  841 CTCCAGACAG GACGTCCAGC ATTATAAGTC TGGACCACGG NATTTGTCTN CAGGCTGGTC
  901 AGTTTAATAG TGTTGTCTAN GGAANCAGAN AGTANCAAGC NNCTNANGNN ANTGCTAAAC
  961 GCCAGTCCNC NGNTCTGNTT GNNNTGCNTN NNNNNGTNNT GNNNGNNNTT CNNGNTNNNN
1021 NNNNNN
//
Primer: CSII_CMV5_v2 (Pr0255)
Region: FLAG, attB1, insert 5' (LQP/AAAx4)
Sequence file: RDB17501_A9Ioc.seq check
>D06551B3_A9Io_1_CSII_CMV5_v2_B02_05_ABI24.ab1
    1 NNNNNNNNNN NNNNGCNGGC NNGCNCCNTG GNNTATAAAG ANGANGACGA TAAGCATGGT
   61 GGTTCTGGTC CAAGCTATCA AACAAGTTTG TACAAAAAAG CAGGCTCCGC GGCCGCCCCC
  121 TTCACCATGG CTCATGAAGC AATGGAATAT GATGTTCAGG TGCAGTTAAA TCATGCCGAA
  181 GCAGCTGCAG CTCCTGCTGG CATGGCCAGC AGCCAAGGGG GACCAGCCCT CGCTGCAGCT
  241 GTTCCTGCTG ATGTGGTCAG CAGCCAGGGG GTACCATCCA TCGCAGCTGC AGCTCCTGCT
  301 GAGGTGATCA GCAGCCAAGC GACACCACCC CTGGCAGCAG CTGCTCCGCA ACTGTCTGTT
  361 GACCTGACAG AAGTGGAGGT CTTGGGAGAA GACACTGTGG AGAACATCAA TCCAAGAACT
  421 TCAGAACAAC ATAGGCAGGG ATCTGATGGT AATCACACCA TCCCAGCATC TTCGTTGCAT
  481 TCAATGACCA ACTTCATCAG CGGACTGCAG AGACTTCATG GCATGCTGGA ATTCCTGAGA
  541 CCTTCATCTT CAAACCACAG TGTAGGGCCA ATGAGAACAA GAAGGAGGGT ATCTGCTTCA
  601 CGGAGGGCAA GAGCCGGAGG GTCTCAGAGG ACAGACAGTG CCAGGTTGAG AGCACCATTG
  661 GATGCTTACT TTCAGGTGAG CAGGACCCAG CCTGACTTGC CAGCTACCAC TTATGATTCA
  721 GAGACTAGGA ATCCTGTATC TGAAGAGTTG CAGGTGTCTA GTAGTTCTGA TTCTGACAGT
  781 GACAGCTCTG CAGAGTATGG ANGGGTTGTT GANCAGGCAG ANGAATCTGG ANCTGTCATT
  841 TTAGAAGANC AACTAGCANG TGTCTCANCA NANCAAGAAG TTACATGTAT CGATGNNNGC
  901 AAGANCNTCC NNNAANAGCN NTCTCCCNNG AANNCTGANC NNNNGCTNCC TTCNGCTTNT
961 NNNGNTNNNN NNNNNN
//
Primer: HIVgp_R1 (Pr0132)
Region: HIV-1 psi, , 5' LTR
Sequence file: RDB17501_A9Iod.seq check
>D06551B3_A9Io_1_HIVgp_R1_D02_11_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNC TCTTTTTTCT CTCTGCACCN CTCTTCTCTT TGCCTTGGTG
   61 GGTGCTACTC CTAATGGTTC AATTTTTACT ACTTTATATT TATATAATTC ACTTCTCCAA
  121 TTGTCCCTCA TATCTCCTCC TCCAGGTCTG AAGATCAGCG GCCGGCCGCT TGCTGTGCGG
  181 TGGTCTTACT TTTGTTTTGC TCTTCCTCTA TCTTGTCTAA AGCTTCCTTG GTGTCTTTTA
  241 TCTCTATCCT TTGATGCACA CAATAGAGGG TTGCTACTGT ATTATATAAT GATCTAAGTT
  301 CTTCTGATCC TGTCTGAAGG GATGGTTGTA GCTGTCCCAG TATTTGTCTA CAGCCTTCTG
  361 ATGTTTCTAA CAGGCCAGGA TTAACTGCGA ATCGTTCTAG CTCCCTGCTT GCCCATACTA
  421 TATGTTTTAA TTTATATTTT TTCTTTCCCC CTGGCCTTAA CCGAATTTTT TCCCATCGCG
  481 ATCTAATTCT CCCCCGCTTA ATACTGACGC TCTCGCACCC ATCTCTCTCC TTCTAGCCTC
  541 CGCTAGTCAA AATTTTTGGC GTACTCACCA GTCGCCGCCC CTCGCCTCTT GCCGTGCGCG
  601 CTTCAGCAAG CCGAGTCCTG CGTCGAGAGA GCTCCTCTGG TTTCCCTTTC GCTTTCAAGT
  661 CCCTGTTCGG GCGCCACTGC TAGAGATTTT CCACACTGAC TAAAAGGGTC TGAGGGATCT
  721 CTAGTTACCA GAGTCACACA ACAGACGGGC ACACACTACT TGAAGCACTC AAGGCAAGCT
  781 TTATTGAGGC TTAAGCAGTG GGTTCCCTAN TTAGCCAGAG AGCTCCCAGG CTCAGATCTG
  841 GTCTAACCAG AGAGACCCAG TACAGGCAAA ACGCGCTGCT TATATAGACC TCCNACNGNA
  901 CACGCNNANC GCCCATTTGC GTCAANGGGG GCGGANTTGT TNCGACATTT TGGAAAGTCC
  961 CNNTGATTTT GGNGCNNAAA CNAANTCCCN TTNNNGNNNN NNGGGGNNGN ANNNTNNNNN
1021 ATCCNNNNNN NNNN
//
Primer: cPPT+CTS_v2_F (Pr0303)
Region: cPPT_CTS, CMV en 2, CMV pro 2
Sequence file: RDB17501_A9Ioe.seq check
>D06551B3_A9Io_1_cPPT+CTS_v2_F_E02_14_ABI24.ab1
    1 NNNNNNNNNN NNNCNNNNNN NNTACNAAAA CNAATTACAA AAATTCAAAA TTTTCGGGTT
   61 TATTACAGGG ACAGCAGAAA TTCACTTTGA ATTAATTCGA TTAATAGTAA TCAATTACGG
  121 GGTCATTAGT TCATAGCCCA TATATGGAGT TCCGCGTTAC ATAACTTACG GTAAATGGCC
  181 CGCCTGGCTG ACCGCCCAAC GACCCCCGCC CATTGACGTC AATAATGACG TATGTTCCCA
  241 TAGTAACGCC AATAGGGACT TTCCATTGAC GTCAATGGGT GGAGTATTTA CGGTAAACTG
  301 CCCACTTGGC AGTACATCAA GTGTATCATA TGCCAAGTAC GCCCCCTATT GACGTCAATG
  361 ACGGTAAATG GCCCGCCTGG CATTATGCCC AGTACATGAC CTTATGGGAC TTTCCTACTT
  421 GGCAGTACAT CTACGTATTA GTCATCGCTA TTACCATGGT GATGCGGTTT TGGCAGTACA
  481 TCAATGGGCG TGGATAGCGG TTTGACTCAC GGGGATTTCC AAGTCTCCAC CCCATTGACG
  541 TCAATGGGAG TTTGTTTTGG CACCAAAATC AACGGGACTT TCCAAAATGT CGTAACAACT
  601 CCGCCCCATT GACGCAAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT ATAAGCAGAG
  661 CTGGTTTAGT GAACCGNNNN ATCCGCTAGG GAGACCCAAG CTGGCTAGCA CCATGGANTA
  721 TAAAGATGAT GACGATAAGC ATGGTGGTTC TGGTCCAAGC TATNNAACAA GTTTGTACNA
  781 AAAAGCAGGC TCCGCGGCCG CCCCCTTCAC CATGGCTCAT GAANCAATGN AATATGATGT
  841 TCAGGTGCNG TTNAATCATG CCGAANCAGC TGCAGCTNCT GCTGGCATGG CCNGCAGNCA
  901 AGGGGNANCA NCCCNCGCTG CANCTGTNCC TGCTNATGTG GNCAGCANCC ANGGGNNNNC
961 ATNCATCNNA GCTGCNGCTN CNNGCTNNNN NGNTCAGCAN NCNNANCNNN NNNNNNN
//
Primer: BSD_R1 (Pr0758)
Region: BSD, IRES2
Sequence file: RDB17501_A9Iof.seq check
>D06551B3_A9Io_1_BSD_R1_G02_20_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNCCNN NNNNNGNNGC GGCCGTCGCT NGAGANAGCT
   61 GCGCTGGCGA CGCTGTAGTC TTCNNAGATG GGGATGCTGT TGATTGTAGC CGTTGCTCTT
  121 TCAATGAGGG TGGATTCTTC TTGAGACAAA GGCTTGGCCA TGGTTGTGGC CATATTATCA
  181 TCGTGTTTTT CAAAGGAAAA CCACGTCCCC GTGGTTCGGG GGGCCTAGAC GTTTTTTTAA
  241 CCTCGACTAA ACACATGTAA AGCATGTGTA CCGAGGCCCC AGATCAGATC CCATACAATG
  301 GGGTACCTTC TGGGCATCCT TCAGCCCCTT GTTGAATACG CTTGAGGAGA GCCATTTGAC
  361 TCTTTCCACA ACTATCCAAC TCACAACGTG GCACTGGGGT TGTGCCGCCT TTGCAGGTGT
  421 ATCTTATACA CGTGGCTTTT GGCCGCAGAG GCACCTGTCG CCAGGTGGGG GGTTCCGCTG
  481 CCTGCAAAGG GTCGCTACAG ACGTTGTTTG TCTTCAAGAA GCTTCCAGAG GAACTGCTTC
  541 CTTCACGACA TTCAACAGAC CTTGCATTCC TTTGGCGAGA GGGGAAAGAC CCCTAGGAAT
  601 GCTCGTCAAG AAGACAGGGC CAGGTTTCCG GGCCCTCACA TTGCCAAAAG ACGGCAATAT
  661 GGTGGAAAAT AACATATAGA CAAACGCACA CCGGCCTTAT TCCAAGCGGC TTCGGCCAGT
  721 AACGTTAGGG GGGGGGGAGG GAGAGGGGCG GATCCGCGGC CGCTGCAGAA TTCTCGATGG
  781 GCAANTANAA NGGNNNNGTC GANGCTGATC AGCGGGNNTT ATCNAAACCA CTTNNNACNN
  841 GNNAANNCTN GNNCGGCGCN NNNCACCCNT TNCNCNNNCN CNTNTANNNG NGNGANNCNT
901 CTTCNCTGNN NANGGNANNC AANNNNNNGN NNNNNNNTCA NCTCNNATGN NNNNNNNNN
//
Primer: BGH_rev2 (Pr0606)
Region: bGH p(A), 3' LTR, WPRE
Sequence file: RDB17501_A9Iog.seq check
>D06551B3_A9Io_1_BGH_rev2_F02_17_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNGGGGGNNG GGCANCAACA GATGGCTGGC AACTAGAAGG
   61 CACAGTCGAG GCTGATCAGC GGGTTTAAAC GGGCCCTGCT AGAGATTTTC CACACTGACT
  121 AAAAGGGTCT GAGGGATCTC TAGTTACCAG AGTCACACAA CAGACGGGCA CACACTACTT
  181 GAAGCACTCA AGGCAAGCTT TATTGAGGCT TAAGCAGTGG GTTCCCTAGT TAGCCAGAGA
  241 GCTCCCAGGC TCAGATCTGG TCTAACCAGA GAGACCCAGT ACAGTCCGGA TGCAGCTCTC
  301 GGGCCATGTG ATGAAATGCT AGGCGGCTGT CAAACCTCCA CTCTAATACT TCTCTCTCCG
  361 GGTCATCCAT CCCATGCAGG CTCACAGGGT GTAACAAGCG GGTGTTCTCT CCTTCATTGG
  421 CTTCTTCTAC CTTCTCTTGC TCAACTGGTA CTAGCTTGTA GCACCATCCA AAGGTCAGTG
  481 GATATCTGAT CCCTGGCCCT GGTGTGTAGT TCTGCCAATC AGGGAAGTAG CCTTGTGTGT
  541 GGTAGATCCA CAGATCAAGG ATATCTTGTC TTCGTTGGGA GTGAATTAGC CCTTCCAGTC
  601 CCCCCTTTTC TTTTAAAAAG TGGCTAAGAT CTACAGCTGC CTTGTAAGTC ATTGGTCTTA
  661 AAGGTACCTG AGGTGTGACT GGAAAACCCA CCTCCTCCTC CTCTTGTGCT TCTAGCCAGG
  721 CACAATCAGC ATTGGTAGCT GCTGTATTGC TACTTGTGAT TGCTCCATGT TTTTCTAGGT
  781 CTCGACGGTA TCGATGCGGG GAGGCGGCCC AAAGGGAGNT CCGANTCGTC TGANGGCGAA
  841 NGCGAANANG CGGAAGANGC CGCANANNCG GCAGCANGNC GCGGGAANGN ANNNCCGCTG
  901 GNNTGANGGN CGAANNGNAN GTANCANAAN NNNGNNCCNC GNANAANTCC NNGTGGNNAC
961 ACAGGNNANC ANNCNNGGAA NNNANGTCNN CNTNCCCNNN NNNNNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori, EM7 pro, BleoR, SV40 p(A)
Sequence file: RDB17501_A9Ioh.seq check
>D06551B3_A9Io_1_SV40pro_ori_F_H02_23_ABI24.ab1
    1 NNNNNNNNNN NNNNNNGCCT CTGCCTCTGA NCTATTCNNG AAGTAGTGAG GAGGCTTTTT
   61 TGGAGGCCTA GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA
  121 TCAGCACGTG TTGACAATTA ATCATCGGCA TAGTATATCG GCATAGTATA ATACGACAAG
  181 GTGAGGAACT AAACCATGGC CAAGTTGACC AGTGCCGTTC CGGTGCTCAC CGCGCGCGAC
  241 GTCGCCGGAG CGGTCGAGTT CTGGACCGAC CGGCTCGGGT TCTCCCGGGA CTTCGTGGAG
  301 GACGACTTCG CCGGTGTGGT CCGGGACGAC GTGACCCTGT TCATCAGCGC GGTCCAGGAC
  361 CAGGTGGTGC CGGACAACAC CCTGGCCTGG GTGTGGGTGC GCGGCCTGGA CGAGCTGTAC
  421 GCCGAGTGGT CGGAGGTCGT GTCCACGAAC TTCCGGGACG CCTCCGGGCC GGCCATGACC
  481 GAGATCGGCG AGCAGCCGTG GGGGCGGGAG TTCGCCCTGC GCGACCCGGC CGGCAACTGC
  541 GTGCACTTCG TGGCCGAGGA GCAGGACTGA CACGTGCTAC GAGATTTCGA TTCCACCGCC
  601 GCCTTCTATG AAAGGTTGGG CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC
  661 CAGCGCGGGG ATCTCATGCT GGAGTTCTTC GCCCACCCCA ACTTGTTTAT TGCAGCTTAT
  721 AATGNTTACA AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG
  781 CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG TATACNGTCN
  841 ANCTCTANCT AGAGCTTGGC GTAATCATGG TCATAGCTGT TNCCTGNGTG AAATTGNTAT
  901 CCGCNCACNA TTNCACACNA CATACGANCC NGAAGCATAA AGTGTAAAGC NNGGGGGNGC
961 CNNNNGANTG AGCNNANNNN CATTNNNNGC NNNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Inano, S., RFWD3-Mediated Ubiquitination Promotes Timely Removal of Both RPA and RAD51 from DNA Damage Sites to Facilitate Homologous Recombination. Mol. Cell 66 (5): 622-634.e8 (2017). PMID 28575658. [link to RRC of NBRP]