Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CSII FLAG-hRFWD3 S46/63A (#RDB17499)

Lentivirus expression vector of human ring finger and WD repeat domain 3 (RFWD3) mutant, S46/63A, non-phosphorylatable mutation.

Clone info. Lentivirus expression vector of human ring finger and WD repeat domain 3 (RFWD3) mutant, S46/63A, non-phosphorylatable mutation, tagged with FLAG at N-terminus, CMV promoter.
Comment Expression was confirmed by the depositor with immunoblotting. Known differences (at least): G to A substitution at 1269nt of NM_018124.4 (R392K; AGG to AAG).
Vector backbone CSII-CMV-MCS-IRES-Bsd (RDB04385) (plasmid)
Selectable markers Lentivirus transfection: Bsd^r. E. coli: Amp^r. Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Growth conditions LB+Ampicillin, 37oC
Gene/insert name Human RFWD3 cDNA
Depositor|Developer Takata, Minoru |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Ordering instruction.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Inano, S et al. Mol. Cell, 66(5): 622-634, 2017)
Additional terms and conditions:
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
Remarks
Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Inano, S et al. Mol. Cell, 66(5): 622-634, 2017)。
付加的使用条件：
本件研究材料は、非営利機関の非営利学術研究に限って提供する。レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
備考
Gateway®テクノロジーを用いた発現クローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17499 CSII FLAG-hRFWD3 S46/63A DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge plus licensing fee equivalent for Gateway® expression clone

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The CSII FLAG-hRFWD3 S46/63A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17499).

Reference section:

Inano, S., Sato, K., Katsuki, Y., Kobayashi, W., Tanaka, H., Nakajima, K., Nakada, S., Miyoshi, H., Knies, K., Takaori-Kondo, A., Schindler, D., Ishiai, M., Kurumizaka, H., Takata, M., RFWD3-Mediated Ubiquitination Promotes Timely Removal of Both RPA and RAD51 from DNA Damage Sites to Facilitate Homologous Recombination. Mol. Cell 66 (5): 622-634.e8 (2017). PMID 28575658. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17499_A9Iop1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: hRFWD3-N_R (Pr0757)
Region: insert 5'(S46A), attB1, FLAG, CMV pro 2, CMV en 2
Sequence file: RDB17499_A9Ioa.seq check
>D06551B1_A9Io_2_hRFWD3-N_R_A02_02_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNC TNNGCNNGNN CTGGCTGGNN GANGGATGGT
   61 ACCCCCTGGG CGCTGACCAC ATCAGCAGGA ACAGGCTGGA GGAGGGCTGG TCCCCCTTGG
  121 CTGCTGGCCA TGCCAGCAGG AGCTGGCTGT TGTTCGGCAT GATTTAACTG CACCTGAACA
  181 TCATATTCCA TTGCTTCATG AGCCATGGTG AAGGGGGCGG CCGCGGAGCC TGCTTTTTTG
  241 TACAAACTTG TTTGATAGCT TGGACCAGAA CCACCATGCT TATCGTCATC ATCTTTATAA
  301 TCCATGGTGC TAGCCAGCTT GGGTCTCCCT AGCGGATCTG ACGGTTCACT AAACCAGCTC
  361 TGCTTATATA GACCTCCCAC CGTACACGCC TACCGCCCAT TTGCGTCAAT GGGGCGGAGT
  421 TGTTACGACA TTTTGGAAAG TCCCGTTGAT TTTGGTGCCA AAACAAACTC CCATTGACGT
  481 CAATGGGGTG GAGACTTGGA AATCCCCGTG AGTCAAACCG CTATCCACGC CCATTGATGT
  541 ACTGCCAAAA CCGCATCACC ATGGTAATAG CGATGACTAA TACGTAGATG TACTGCCAAG
  601 TAGGAAAGTC CCATAAGGTC ATGTACTGGG CATAATGCCA GGCGGGCCAT TTACCGTCAT
  661 TGACGTCAAT AGGGGGCGTA CTTGGCATAT GATACACTTG ATGTACTGCC AAGTGGGCAG
  721 TTTACCGTAA ATACTCCACC CATTGACGTC AATGGAAAGT CCCTATTGGC GTTACTATGG
  781 GAACATACGT CATTATTGAC GTCAATGGGC GGGGGTCGTT GGGCGGTCAG CCAGGCGGGC
  841 CATTTACCGT AAGTTATGTA ACGCGGAACT CCATATATGG GCTATGAACT AATGANCCNG
  901 TAATTGATTA CTATTAATCG AANNNATTNN AAGTGAATTN CTGCTGNCNN TGNNNTAAAN
  961 CCGAAAATTT NNAANTTTTN GTNNNTTGNT TTTGNNANTN NNTTANTTNG NANNNNNNNN
1021 NN
//
Primer: IRES_R3 (Pr0573)
Region: attB2, insert 3'
Sequence file: RDB17499_A9Iob.seq check
>D06551B1_A9Io_2_IRES_R3_E06_15_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNGCGGNN CCGCGGCCGC TGCNNAATTC
   61 TCGATGGCAA CTAGAAGGCA CAGTCGAGGC TGATCAGCGG GTTTATCGAA CCACTTTGTA
  121 CAAGAAAGCT GGGTCGGCGC GCCCACCCTT TCACTCCCAC TTATAGATGT GGACCATCTT
  181 CTCTGTTAAG GTAGCCAAGT AGCTGTTACG GTTCACCTCA AATGGGCAGA TGTCCAACAC
  241 AGGCTGATCG GTCTGTAGGT CCTGGAGCAA CGAGCCACTG GCAGCATCCC ACAGCAGGGC
  301 AGAATTTGCT GCTTCATCCC CAGTACACAC CAGGATGTTG CCATCATTCT CTGGGCTTTG
  361 GAAAATGGCA TTTTTGGTCA ATAGTTTGCA AGTAGGTCCT CCAAAAAATG TATGTACAGG
  421 CTGGCAGGAG CAGATTGGAT TTCCAGTGTC ATCCAGTCGG TAGGACATTT CCATCAGCAC
  481 ACTTCGTATG GTGGTGTGAT TTTTATCAGG CCTGTAGGTC ACAAGACAGT GCCGGGAGCT
  541 GTTCTCTGTC TGAAAGTCTA TGCAGCCCCC TGGCTCCAAG GGCAGCACAT GAGGCCAATG
  601 AGAAAAGTCC ATTTTCTGTT CCCAGAATGA AGCATCCTCC AAGGTTCCAG CCAGCACCCC
  661 ACCATATGGA AATGCAGCTG AGGCAGCTCT GGGCATGTAT GACAGGGAGA CCAGTGGGCA
  721 TCTGGCTTTC TGANCTACTA ACTCCTGCAC ATGACTGCTC GTGTTTCGCA CGTCNTATAC
  781 CANANTTGAA CCATTGGCCA GTCCANNATA GATGTNNNTA NCCTCATCAN NACNCCAGCA
  841 ACAGCTCCNG ACAGGACGTN CAGCATTANN AAGTCTGGAN CNCGGTATTT GTCTCCNNGC
  901 TGGTCAGNTN NATANNGTTG TCTAGGGNAN CANANAGTAG NNAGCCTCTG ANGNANNNGC
  961 NNAACGCCNN NNCNCGGATC TGTTTNNCNT GCNNCGGAAN TGNNCTGNNN GNNNNTNNNN
1021 NNNGNCAGNN NNNNNNNTNN TNNNNNNNNN AANNNNNNNN NN
//
Primer: CSII_CMV5_v2 (Pr0255)
Region: FLAG, attB1, insert 5' (S46A, S63A)
Sequence file: RDB17499_A9Ioc.seq check
>D06551B1_A9Io_2_CSII_CMV5_v2_C01_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NGGCNNNCNN CNTGGNNTAT AAAGANGATG ACGATAAGCA
   61 TGGTGGTTCT GGTCCAAGCT ATCAAACAAG TTTGTACAAA AAAGCAGGCT CCGCGGCCGC
  121 CCCCTTCACC ATGGCTCATG AAGCAATGGA ATATGATGTT CAGGTGCAGT TAAATCATGC
  181 CGAACAACAG CCAGCTCCTG CTGGCATGGC CAGCAGCCAA GGGGGACCAG CCCTCCTCCA
  241 GCCTGTTCCT GCTGATGTGG TCAGCGCCCA GGGGGTACCA TCCATCCTCC AGCCAGCTCC
  301 TGCTGAGGTG ATCAGCGCCC AAGCGACACC ACCCCTGCTC CAGCCTGCTC CGCAACTGTC
  361 TGTTGACCTG ACAGAAGTGG AGGTCTTGGG AGAAGACACT GTGGAGAACA TCAATCCAAG
  421 AACTTCAGAA CAACATAGGC AGGGATCTGA TGGTAATCAC ACCATCCCAG CATCTTCGTT
  481 GCATTCAATG ACCAACTTCA TCAGCGGACT GCAGAGACTT CATGGCATGC TGGAATTCCT
  541 GAGACCTTCA TCTTCAAACC ACAGTGTAGG GCCAATGAGA ACAAGAAGGA GGGTATCTGC
  601 TTCACGGAGG GCAAGAGCCG GAGGGTCTCA GAGGACAGAC AGTGCCAGGT TGAGAGCACC
  661 ATTGGATGCT TACTTTCAGG TGAGCAGGAC CCAGCCTGAC TTGCCAGCTA CCACTTATGA
  721 TTCAGAGACT AGGAATCCTG TATCTGAAGA GTTGCAGGTG TCTAGTAGTT CTGATTCTGA
  781 CAGTGACAGC TCTGCAGANT ATGGAGGGGT TGTTGANCAG GCAGANGAAT CTGGANCTGT
  841 CATTTTAGAA GANCAACTAG CANGTGTCTC AGCAGANCAA GAAGTTNCAT GTATCGATGG
  901 ANGCAAGANC NNCCCCAAAC AGCCATCTCC CCANAANNNT GANCCNCTGC NANCTTCTGC
  961 TTCTATGGAT GANGNAAGNN NNGGNANNNN TGNNNNNTAT GTCNNGNAAN NGNNNNNNNA
1021 NNNNNNGGGN NNNNNNNNN
//
Primer: HIVgp_R1 (Pr0132)
Region: HIV-1 psi, 5' LTR
Sequence file: RDB17499_A9Iod.seq check
>D06551B1_A9Io_2_HIVgp_R1_E01_13_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNGC TCTTTTTTCT CTCTGCNCNN CTCTTCTCTT TGCCTTGGTG
   61 GGTGCTACTC CTAATGGTTC AATTTTTACT ACTTTATATT TATATAATTC ACTTCTCCAA
  121 TTGTCCCTCA TATCTCCTCC TCCAGGTCTG AAGATCAGCG GCCGGCCGCT TGCTGTGCGG
  181 TGGTCTTACT TTTGTTTTGC TCTTCCTCTA TCTTGTCTAA AGCTTCCTTG GTGTCTTTTA
  241 TCTCTATCCT TTGATGCACA CAATAGAGGG TTGCTACTGT ATTATATAAT GATCTAAGTT
  301 CTTCTGATCC TGTCTGAAGG GATGGTTGTA GCTGTCCCAG TATTTGTCTA CAGCCTTCTG
  361 ATGTTTCTAA CAGGCCAGGA TTAACTGCGA ATCGTTCTAG CTCCCTGCTT GCCCATACTA
  421 TATGTTTTAA TTTATATTTT TTCTTTCCCC CTGGCCTTAA CCGAATTTTT TCCCATCGCG
  481 ATCTAATTCT CCCCCGCTTA ATACTGACGC TCTCGCACCC ATCTCTCTCC TTCTAGCCTC
  541 CGCTAGTCAA AATTTTTGGC GTACTCACCA GTCGCCGCCC CTCGCCTCTT GCCGTGCGCG
  601 CTTCAGCAAG CCGAGTCCTG CGTCGAGAGA GCTCCTCTGG TTTCCCTTTC GCTTTCAAGT
  661 CCCTGTTCGG GCGCCACTGC TAGAGATTTT CCACACTGAC TAAAAGGGTC TGANGGATCT
  721 CTAGTTACCA GAGTCACACA ACAGACGGGC ACACACTACT TGAAGCACTC AAGGCAAGCT
  781 TTATTGAGGC TTAAGCAGTG GGTTCCCTAG TTAGCCAGAN AGCTCCCANG CTCAGATCTG
  841 GTCTAACCAG AGAGACCCAG TACANGCAAA ACGCGCTGCT TATATAGACC TCCNNCCGTA
  901 CNCGCCTACN GCCNNTTTGC GTCAATGGGG GCGGANTTGT NNCNANATTT TGGAAANTCC
  961 CNNNGANTTT GGTGNCNAAA NNAACTCCCN NNGNNNNNNN NGGGGNGGNN NNTNNNNNAN
1021 NCCCNNNNNN TCNANNNNNN NNNN
//
Primer: cPPT+CTS_v2_F (Pr0303)
Region: cPPT_CTS, CMV en 2, CMV pro 2
Sequence file: RDB17499_A9Ioe.seq check
>D06551B1_A9Io_2_cPPT+CTS_v2_F_F01_16_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNANNAATT ACAAAAATTC AAAATTTTCG
   61 GGTTTATTAC AGGGACAGCA GAAATTCACT TTGAATTAAT TCGATTAATA GTAATCAATT
  121 ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT
  181 GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT
  241 CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA
  301 ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA GTACGCCCCC TATTGACGTC
  361 AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA TGACCTTATG GGACTTTCCT
  421 ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG
  481 TACATCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT
  541 GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAC
  601 AACTCCGCCC CATTGACGCA AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC
  661 AGAGCTGGTT TAGTGAACCN NNNNATCCGC TAGGGAGACC CAAGCTGGCT AGCACCATGG
  721 ATTATAAAGA TGATGACGAT AAGCATGGTG GTTCTGGTCC AAGCTATCNN ACAAGTTTGT
  781 ACAAAAAAGC AGGCTCCGCG GCCGCCCCCT TCACCATGGC TCATGAAGCA ATGNAATATG
  841 ATGNTCAGGT GCAGTTAAAT CATGCCNAAC NACAGCCAGC TNCNGCTGGC ATGGNCAGCA
  901 GNNNANGGGG ANCANNCCNN CTCCANCCNG TNNCTGCTGA TGTGGTCANC GNCNNNGGGG
961 NANCATNCAT CCTNCANCNN NNNNCNGNNN NNNNNTCAGC GNCNAANCNN NNNNNNNN
//
Primer: IRES-F (Pr0036)
Region: IRES2, BSD, WPRE
Sequence file: RDB17499_A9Iof.seq check
>D06551B1_A9Io_2_IRES-F_H01_22_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NCNAGGTNNN NNNNACGTCT AGGCCCCCCG AACCNCGGGG
   61 ACGTGGTTTT CCTTTGAAAA ACACGATGAT AATATGGCCA CAACCATGGC CAAGCCTTTG
  121 TCTCAAGAAG AATCCACCCT CATTGAAAGA GCAACGGCTA CAATCAACAG CATCCCCATC
  181 TCTGAAGACT ACAGCGTCGC CAGCGCAGCT CTCTCTAGCG ACGGCCGCAT CTTCACTGGT
  241 GTCAATGTAT ATCATTTTAC TGGGGGACCT TGTGCAGAAC TCGTGGTGCT GGGCACTGCT
  301 GCTGCTGCGG CAGCTGGCAA CCTGACTTGT ATCGTCGCGA TCGGAAATGA GAACAGGGGC
  361 ATCTTGAGCC CCTGCGGACG GTGCCGACAG GTGCTTCTCG ATCTGCATCC TGGGATCAAA
  421 GCCATAGTGA AGGACAGTGA TGGACAGCCG ACGGCAGTTG GGATTCGTGA ATTGCTGCCC
  481 TCTGGTTATG TGTGGGAGGG CTAAGTCGAG CCTCTAGAGT TAACATCGAG GGATCAAGCT
  541 TATCGATAAT CAACCTCTGG ATTACAAAAT TTGTGAAAGA TTGACTGGTA TTCTTAACTA
  601 TGTTGCTCCT TTTACGCTAT GTGGATACGC TGCTTTAATG CCTTTGTATC ATGCTATTGC
  661 TTCCCGTATG GCTTTCATTT TCTCCTCCTT GTATAAATCC TGGTTGCTGT CTCTTTATGA
  721 GGAGTTGTGG CCCGTTGTCA GGCAACGTGG CGTGGTGTGC ACTGTGTTTG CTGACGCAAC
  781 CCCCACTGGT TGGGGCATTG CCACCACCTG TCAGCTCCTT TCCGGGANTT TCGCTTTCCC
  841 CCTCCCNATT GCCACGGCGG AACTCATCGC CGCCTGCCTT GCCCGCTGCT GGANAGGGGC
  901 TCGGCTGTTG GGCACTGACN ATTNCGTGGT GTTGNCGGGG AANCTGANGT CCTTTNCNTG
961 GCTGCNNGCC TGNGTTGCNN NNNNNNNN
//
Primer: BGH_rev2 (Pr0606)
Region: bGH p(A), 3' LTR, WPRE
Sequence file: RDB17499_A9Iog.seq check
>D06551B1_A9Io_2_BGH_rev2_G01_19_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNGNNG GGCAAACNAC AGATGGCTGG CAACTAGAAG
   61 GCACAGTCGA GGCTGATCAG CGGGTTTAAA CGGGCCCTGC TAGAGATTTT CCACACTGAC
  121 TAAAAGGGTC TGAGGGATCT CTAGTTACCA GAGTCACACA ACAGACGGGC ACACACTACT
  181 TGAAGCACTC AAGGCAAGCT TTATTGAGGC TTAAGCAGTG GGTTCCCTAG TTAGCCAGAG
  241 AGCTCCCAGG CTCAGATCTG GTCTAACCAG AGAGACCCAG TACAGTCCGG ATGCAGCTCT
  301 CGGGCCATGT GATGAAATGC TAGGCGGCTG TCAAACCTCC ACTCTAATAC TTCTCTCTCC
  361 GGGTCATCCA TCCCATGCAG GCTCACAGGG TGTAACAAGC GGGTGTTCTC TCCTTCATTG
  421 GCTTCTTCTA CCTTCTCTTG CTCAACTGGT ACTAGCTTGT AGCACCATCC AAAGGTCAGT
  481 GGATATCTGA TCCCTGGCCC TGGTGTGTAG TTCTGCCAAT CAGGGAAGTA GCCTTGTGTG
  541 TGGTAGATCC ACAGATCAAG GATATCTTGT CTTCGTTGGG AGTGAATTAG CCCTTCCAGT
  601 CCCCCCTTTT CTTTTAAAAA GTGGCTAAGA TCTACAGCTG CCTTGTAAGT CATTGGTCTT
  661 AAAGGTACCT GAGGTGTGAC TGGAAAACCC ACCTCCTCCT CCTCTTGTGC TTCTAGCCAG
  721 GCACAATCAG CATTGGTAGC TGCTGTATTG CTACTTGTGA TTGCTCCATG TTTTTCTAGG
  781 TCTCGACGGT ATCGATGCGG GGAGGCGGCC CAAAGGGAGA TCCGACTCGT CTGANGGCGA
  841 ANGCGAAGAN GCGGAAGANG CCGCANANNN GGCAGCANGC CGCGGGAANG AAGGTCCGCT
  901 GNNNTGANGG CCGNANGGNN NGTANCNNAA NGNCGNNCCG CGCAGAATCC NNGNTGGNNN
  961 CACANGNNGA NCAGCCNTGG AANNNNNNNC AGNNNNCCCG ANNNCACCAC NGNANNNNNN
1021 NNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori, EM7 pro, BleoR, SV40 p(A)
Sequence file: RDB17499_A9Ioh.seq check
>D06551B1_A9Io_2_SV40pro_ori_F_A02_02_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNGCC NCTGCCTCTG AGCTNTTCNN GAAGTAGTGA GGAGGCTTTT
   61 TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG
  121 ATCAGCACGT GTTGACAATT AATCATCGGC ATAGTATATC GGCATAGTAT AATACGACAA
  181 GGTGAGGAAC TAAACCATGG CCAAGTTGAC CAGTGCCGTT CCGGTGCTCA CCGCGCGCGA
  241 CGTCGCCGGA GCGGTCGAGT TCTGGACCGA CCGGCTCGGG TTCTCCCGGG ACTTCGTGGA
  301 GGACGACTTC GCCGGTGTGG TCCGGGACGA CGTGACCCTG TTCATCAGCG CGGTCCAGGA
  361 CCAGGTGGTG CCGGACAACA CCCTGGCCTG GGTGTGGGTG CGCGGCCTGG ACGAGCTGTA
  421 CGCCGAGTGG TCGGAGGTCG TGTCCACGAA CTTCCGGGAC GCCTCCGGGC CGGCCATGAC
  481 CGAGATCGGC GAGCAGCCGT GGGGGCGGGA GTTCGCCCTG CGCGACCCGG CCGGCAACTG
  541 CGTGCACTTC GTGGCCGAGG AGCAGGACTG ACACGTGCTA CGAGATTTCG ATTCCACCGC
  601 CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT
  661 CCAGCGCGGG GATCTCATGC TGGAGTTCTT CGCCCACCCC AACTTGTTTA TTGCAGCTTA
  721 TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT
  781 GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT GTATACCGTC
  841 NANCTCTANC TAGAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA
  901 TCCGCTCACA ATTNNNCACA ACATACGANC CGGAANCATA AAGTGTAAAG CCNGGGGGNG
  961 NCTNANGANT GANCTAACTC NCATNNNTNG CGTTNCNNNN NNNNNCNNNN TTNNNNNNNG
1021 GNNANNNNNN NNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Inano, S., RFWD3-Mediated Ubiquitination Promotes Timely Removal of Both RPA and RAD51 from DNA Damage Sites to Facilitate Homologous Recombination. Mol. Cell 66 (5): 622-634.e8 (2017). PMID 28575658. [link to RRC of NBRP]