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CSII FLAG-hRFWD3 I639K (#RDB17498)

Lentivirus expression vector of human ring finger and WD repeat domain 3 (RFWD3) mutant, I639K, chromatin localization-defective mutation.

Clone info. Lentivirus expression vector of human ring finger and WD repeat domain 3 (RFWD3) mutant, I639K, chromatin localization-defective mutation, tagged with FLAG at N-terminus, CMV promoter.
Comment Expression was confirmed by the depositor with immunoblotting. Known differences (at least): G to A substitution at 1269nt of NM_018124.4 (R392K; AGG to AAG).
Vector backbone CSII-CMV-MCS-IRES-Bsd (RDB04385) (plasmid)
Selectable markers Lentivirus transfection: Bsd^r. E. coli: Amp^r. Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Growth conditions LB+Ampicillin, 37oC
Gene/insert name Human RFWD3 cDNA
Depositor|Developer Takata, Minoru |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Ordering instruction.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Inano, S et al. Mol. Cell, 66(5): 622-634, 2017)
Additional terms and conditions:
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
Remarks
Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Inano, S et al. Mol. Cell, 66(5): 622-634, 2017)。
付加的使用条件：
本件研究材料は、非営利機関の非営利学術研究に限って提供する。レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
備考
Gateway®テクノロジーを用いた発現クローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17498 CSII FLAG-hRFWD3 I639K DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge plus licensing fee equivalent for Gateway® expression clone

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The CSII FLAG-hRFWD3 I639K was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17498).

Reference section:

Inano, S., Sato, K., Katsuki, Y., Kobayashi, W., Tanaka, H., Nakajima, K., Nakada, S., Miyoshi, H., Knies, K., Takaori-Kondo, A., Schindler, D., Ishiai, M., Kurumizaka, H., Takata, M., RFWD3-Mediated Ubiquitination Promotes Timely Removal of Both RPA and RAD51 from DNA Damage Sites to Facilitate Homologous Recombination. Mol. Cell 66 (5): 622-634.e8 (2017). PMID 28575658. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17498_A9Iop1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: hRFWD3-N_R (Pr0757)
Region: insert 5', attB1, CMV pro 2, CMV en 2
Sequence file: RDB17498_A9Ioa.seq check
>D06551A9_A9Io_3_hRFWD3-N_R_G01_19_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNCTGGCT GGNNGNNNNG
   61 ANGGTACCCC CTGGCTGCTG ACCACATCAG CAGGAACAGG CTGGAGGAGG GCTGGTCCCC
  121 CTTGGCTGCT GGCCATGCCA GCAGGAGCTG GCTGTTGTTC GGCATGATTT AACTGCACCT
  181 GAACATCATA TTCCATTGCT TCATGAGCCA TGGTGAAGGG GGCGGCCGCG GAGCCTGCTT
  241 TTTTGTACAA ACTTGTTTGA TAGCTTGGAC CAGAACCACC ATGCTTATCG TCATCATCTT
  301 TATAATCCAT GGTGCTAGCC AGCTTGGGTC TCCCTAGCGG ATCTGACGGT TCACTAAACC
  361 AGCTCTGCTT ATATAGACCT CCCACCGTAC ACGCCTACCG CCCATTTGCG TCAATGGGGC
  421 GGAGTTGTTA CGACATTTTG GAAAGTCCCG TTGATTTTGG TGCCAAAACA AACTCCCATT
  481 GACGTCAATG GGGTGGAGAC TTGGAAATCC CCGTGAGTCA AACCGCTATC CACGCCCATT
  541 GATGTACTGC CAAAACCGCA TCACCATGGT AATAGCGATG ACTAATACGT AGATGTACTG
  601 CCAAGTANGA AAGTCCCATA AGGTCATGTA CTGGGCATAA TGCCAGGCGG GCCATTTACC
  661 GTCATTGACG TCAATAGGGG GCGTACTTGG CATATGATAC ACTTGATGTA CTGCCAAGTG
  721 GGCAGTTTAC CGTAAATACT CNNCCCATTG ACGTCAATGG AAAGTCCCTA TTGNCGTTNC
  781 TATGGGAACA TACGTCNTTA TNNNNNNCNA NNGGGCGGGG GGTCGTTGGG CGGNCAGCCA
  841 GGCGGGCCAT TTACCGNAAG TTATGTNACG CGGNANNTNC ATATATGGGC TATGANCTAN
  901 TGACCCCNNN ATTGANTNAC TATTAATCNA ATTNNNTNNN AAGTGAATTT CTGCTGTNCC
  961 TGTAATAAAC CCGAAAAATT TTNNANTTTT GNNANTTGNT TTTGNNANTN NNTNNNNTNG
 1021 NATGTCNGNN NCTANNATGT CNACNANTNN NTTCCCNNNC NNNNNNNCCC CCNNNNNCNC
1081 CNNTTNNNTT TNNAANNNNN NNNNN
//
Primer: IRES_R3 (Pr0573)
Region: attB2, insert 3' (I639K)
Sequence file: RDB17498_A9Iob.seq check
>D06551A9_A9Io_3_IRES_R3_B02_05_ABI24.ab1
    1 NNNNNNNNNN NGGNNNNNNG NNNGGNNNNG GGCGGNNCCG CGGCCGCTGC AGAATTCTCG
   61 ATGGCAACTA GAAGGCACAG TCGAGGCTGA TCAGCGGGTT TATCGAACCA CTTTGTACNA
  121 GAAAGCTGGG TCGGCGCGCC CACCCTTTCA CTCCCACTTA TAGATGTGGA CCATCTTCTC
  181 TGTTAAGGTA GCCAAGTAGC TGTTACGGTT CACCTCAAAT GGGCAGATGT CCAACACAGG
  241 CTGATCGGTC TGTAGGTCCT GGAGCAACGA GCCACTGGCA GCATCCCACA GCAGGGCAGA
  301 ATTTGCTGCT TCATCCCCAG TACACACCAG GATGTTGCCA TCATTCTCTG GGCTTTGGAA
  361 AATGGCATTT TTGGTCAATA GTTTGCAAGT AGGTCCTCCA AAAAATGTAT GTACAGGCTG
  421 GCAGGAGCAG ATTGGATTTC CAGTGTCATC CAGTCGGTAG GACATTTCCA TCAGCACACT
  481 TCGTATGGTG GTGTGATTTT TATCAGGCCT GTAGGTCACA AGACAGTGCC GGGAGCTGTT
  541 CTCTGTCTGA AAGTCTTTGC ACCCCCCTGG CTCCAAGGGC AGCACATGAG GCCAATGAGA
  601 AAAGTCCATT TTCTGTTCCC AGAATGAAGC ATCCTCCAAG GTTCCAGCCA GCACCCCACC
  661 ATATGGAAAT GCAGCTGAGG CAGCTCTGGG CATGTATGAC AGGGAGANCA GTGGGCATCT
  721 GGCTTTCTGA GCTACTAACT CCTGCACATG ACTGCTCGTG TTTCGCACGT CATATACCAG
  781 AATTGAACCA TTGGCCAGTC CAGCATAGAT GTAGTTAGCC TCATCAAGAC ACCAGCAACA
  841 GCTCCAGACA GGACGTCCAG CATTATAAGT CTGGACCACG GNATTTGTCT CCAGGCTGGN
  901 CAGTTTAATA GTGTTGNCTA NGGAANCANA NANTANCNAG CCTCTGANGT AACTGCTAAA
  961 CGCCANNNCN CGGNTCNGTT TGNCNTGNAT NGGANNGNAN TGNNNGNNNN TCANGNNNNN
1021 NNNNN
//
Primer: hRFWD3-N_F (Pr0753)
Region: insert mid
Sequence file: RDB17498_A9Ioc.seq check
>D06551A9_A9Io_3_hRFWD3-N_F_H04_22_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNTCTGANAN TTGCAGGTGT CTAGTAGTTC TGATTCTGAC
   61 AGTGACAGCT CTGCAGAGTA TGGAGGGGTT GTTGACCAGG CAGAGGAATC TGGAGCTGTC
  121 ATTTTAGAAG AGCAACTAGC AGGTGTCTCA GCAGAGCAAG AAGTTACATG TATCGATGGA
  181 GGCAAGACCC TCCCCAAACA GCCATCTCCC CAGAAGTCTG AGCCTCTGCT ACCTTCTGCT
  241 TCTATGGATG AGGAAGAAGG GGACACTTGT ACAATATGTC TGGAACAGTG GACCAATGCT
  301 GGGGACCACC GGCTCTCAGC ATTACGCTGT GGGCATCTCT TTGGGTATAG GTGCATTTCC
  361 ACGTGGCTTA AAGGACAAGT ACGAAAATGT CCCCAGTGCA ACAAGAAAGC CAGGCACAGT
  421 GACATTGTCG TCCTTTATGC CCGAACCCTG AGAGCTTTGG ACACTAGTGA ACAGGAGCGC
  481 ATGAAAAGTT CCCTACTGAA GGAACAGATG CTAAGGAAAC AGGCCGAGTT AGAATCAGCA
  541 CAGTGCCGAC TCCAACTGCA GGTCCTCACT GATAAGTGCA CTAAGCTTCA AAGGCGTGTT
  601 CAGGACTTGC AAAAACTTAC GTCACATCAA AGTCAGAATT TACAGCAACC CAGGGGCTCC
  661 CAAGCATGGG TCCTGAGCTG CTCACCCTCC AGCCAGGGCC AGCACAAGCA CAAGTACCAC
  721 TTCCAAAAGA CCTTCACAGT ATCTCANGCA GGAAACTGCC GGATCATGGC ATACTGTGAT
  781 GCTCTGANCT GCCTGGTGAT ATCACAGCCT TCTCCTCANG CCTCTTTTCT TCCNGGCTTT
  841 GGTGTTAAGA TGNTGAGTAC TGCCNACATG AAGAGCAGNC NNNACATTCC GATGCATGGC
  901 AAACAGANCC GNGNACNGGC GTTTANCAGT NANCTCANAN GCTTGCTACT CTCTGCTTNC
  961 CTAGACAACA CNATTNANNT GANNNNCCNN NNNNNNAATA CNNNGGNNCN NANNNNNNNN
1021 GNNNGNNNNN NNNNN
//
Primer: HIVgp_R1 (Pr0132)
Region: HIV-1 psi, 5' LTR
Sequence file: RDB17498_A9Iod.seq check
>D06551A9_A9Io_3_HIVgp_R1_E03_15_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NGCTCTTTTT TCTCTCTGCN CCACTCTTCT CTTTGCCTTG
   61 GTGGGTGCTA CTCCTAATGG TTCAATTTTT ACTACTTTAT ATTTATATAA TTCACTTCTC
  121 CAATTGTCCC TCATATCTCC TCCTCCAGGT CTGAAGATCA GCGGCCGGCC GCTTGCTGTG
  181 CGGTGGTCTT ACTTTTGTTT TGCTCTTCCT CTATCTTGTC TAAAGCTTCC TTGGTGTCTT
  241 TTATCTCTAT CCTTTGATGC ACACAATAGA GGGTTGCTAC TGTATTATAT AATGATCTAA
  301 GTTCTTCTGA TCCTGTCTGA AGGGATGGTT GTAGCTGTCC CAGTATTTGT CTACAGCCTT
  361 CTGATGTTTC TAACAGGCCA GGATTAACTG CGAATCGTTC TAGCTCCCTG CTTGCCCATA
  421 CTATATGTTT TAATTTATAT TTTTTCTTTC CCCCTGGCCT TAACCGAATT TTTTCCCATC
  481 GCGATCTAAT TCTCCCCCGC TTAATACTGA CGCTCTCGCA CCCATCTCTC TCCTTCTAGC
  541 CTCCGCTAGT CAAAATTTTT GGCGTACTCA CCAGTCGCCG CCCCTCGCCT CTTGCCGTGC
  601 GCGCTTCAGC AAGCCGAGTC CTGCGTCGAG AGAGCTCCTC TGGTTTCCCT TTCGCTTTCA
  661 AGTCCCTGTT CGGGCGCCAC TGCTAGAGAT TTTCCACACT GACTAAAAGG GTCTGAGGGA
  721 TCTCTAGTTA CCAGAGTCAC ACAACAGACG GGCACACACT ACTTGAAGCA CTCNANGCAA
  781 GCTTTATTGA GGNTTAAGCA GTGGGTTCCC TANTTAGCCN GAGAGCTCCC AGGCTCAGAT
  841 CTGGTCTNAC CAGAGAGANC CAGTACAGGC AAAACGCGCT GCTTATATAG ACCTNCNNCC
  901 GNACNCGCCT ACCGCCNNTT TGCGNCNANG GGGCGGANTT GTTACNACNT TTTGGAAAGT
  961 CCCGTNNANT TTGGNGNNNA AANNNNTCCC ATNGNCNTNN NTGGGNGNNG NANNNNNNNA
1021 ANTCCCNNNN AGNNNNNCNN NNTNTNNNNN NNNNN
//
Primer: cPPT+CTS_v2_F (Pr0303)
Region: cPPT_CTS, CMV en 2, CMV pro 2
Sequence file: RDB17498_A9Ioe.seq check
>D06551A9_A9Io_3_cPPT+CTS_v2_F_B06_06_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN GNNNTACNAA AACNAATTAC AAAAATTCAA AATTTTCGGG
   61 TTTATTACAG GGACAGCAGA AATTCACTTT GAATTAATTC GATTAATAGT AATCAATTAC
  121 GGGGTCATTA GTTCATAGCC CATATATGGA GTTCCGCGTT ACATAACTTA CGGTAAATGG
  181 CCCGCCTGGC TGACCGCCCA ACGACCCCCG CCCATTGACG TCAATAATGA CGTATGTTCC
  241 CATAGTAACG CCAATAGGGA CTTTCCATTG ACGTCAATGG GTGGAGTATT TACGGTAAAC
  301 TGCCCACTTG GCAGTACATC AAGTGTATCA TATGCCAAGT ACGCCCCCTA TTGACGTCAA
  361 TGACGGTAAA TGGCCCGCCT GGCATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC
  421 TTGGCAGTAC ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA
  481 CATCAATGGG CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA
  541 CGTCAATGGG AGTTTGTTTT GGCACCAAAA TCAACGGGAC TTTCCAAAAT GTCGTAACAA
  601 CTCCGCCCCA TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT ATATAAGCAG
  661 AGCTGGTTTA GTGAACCGNN NNATCCGCTA GGGAGANCCN NNNTGGCTAG CANNNTGGAT
  721 TATAAAGATG ATGACGATAA GCATGGTGGT TCTGGTCCNA GCTATCAAAC AAGTTTGTAC
  781 AAAAAAGCAN GCTCCNCGGN CGCNNNNTTC ACCNTGGCTC ATGAANCANT GNANTATGAN
  841 GTTCANGTGC AGTTAAATCA TGCCGAACNA CAGCCAGCTC CTGCTGGCAT GGNCCNNCAN
  901 CCAANGGGGA NNANCCCTCC NNCANNNNGT NCCTGCTNNN GTGGTCANCN NCCNNGGGGN
961 ANCNNTCCNT CCTNNNNNNN GNNNNNGCTG ANGNGNNCAN NNNNNNNNCN NNNNNNNNN
//
Primer: IRES-F (Pr0036)
Region: IRES2, BSD, WPRE
Sequence file: RDB17498_A9Iof.seq check
>D06551A9_A9Io_3_IRES-F_F02_17_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNAACG TCTAGGCCCC CCGAACCACG
   61 GGGACGTGGT TTTCCTTTGA AAAACACGAT GATAATATGG CCACAACCAT GGCCAAGCCT
  121 TTGTCTCAAG AAGAATCCAC CCTCATTGAA AGAGCAACGG CTACAATCAA CAGCATCCCC
  181 ATCTCTGAAG ACTACAGCGT CGCCAGCGCA GCTCTCTCTA GCGACGGCCG CATCTTCACT
  241 GGTGTCAATG TATATCATTT TACTGGGGGA CCTTGTGCAG AACTCGTGGT GCTGGGCACT
  301 GCTGCTGCTG CGGCAGCTGG CAACCTGACT TGTATCGTCG CGATCGGAAA TGAGAACAGG
  361 GGCATCTTGA GCCCCTGCGG ACGGTGCCGA CAGGTGCTTC TCGATCTGCA TCCTGGGATC
  421 AAAGCCATAG TGAAGGACAG TGATGGACAG CCGACGGCAG TTGGGATTCG TGAATTGCTG
  481 CCCTCTGGTT ATGTGTGGGA GGGCTAAGTC GAGCCTCTAG AGTTAACATC GAGGGATCAA
  541 GCTTATCGAT AATCAACCTC TGGATTACAA AATTTGTGAA ANANTGACTG GTATTCTTAA
  601 CTATGTTGCT CCTTTTACGC TATGTGGATA CGCTGCTTTA ATGCCTTTGT ATCATGCTAT
  661 TGCTTCCCGT ATGGCTTTCA TTTTCTCCTC CTTGTATAAA TCCTGGTTGC TGTCTCTTTA
  721 TGAGGAGTTG TGGCCCGTTG TCAGGCAACG TGGCGTGGTG TGCACTGTGT TTGCTGACNN
  781 NNCCCCCACT GGTTGGGGCN TTGNCACCAC CTGTCAGCTC CTTTCCNGGN ANTTTNNNCT
  841 TTCCCCCTCC CTATTGCNNN NNNNNGAANT CATCGNCGCC TGCCTNGCCC GCTGCTGGAC
  901 NNGGGGNTCG GCTGTTGGGN NNNTGACAAT TNCGTGGNGT TNNNCNGGGG AAANCTGANG
  961 NCCTTTNNNA TGGGCTNNNT CNNCNNGNGT TNGCCNCCNN GNNNTTNNNG NGCGGGNNNN
 1021 TNCNTTNCTG CTACNNNCCN TTCNNNNCNT NNANNCNNNN NNGGNANCNT NCNTTNCNNG
1081 NNNNNCNNNN NNNNNN
//
Primer: f1ori_R3 (Pr0142)
Region: bGH p(A), 3' LTR
Sequence file: RDB17498_A9Iog.seq check
>D06551A9_A9Io_3_f1ori_R3_G03_21_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NCNNNNNNTT TCNNCCTCNG AAGCCNTAGA GCCCACCGCA
   61 TCCCCAGCAT GCCTGCTATT GTCTTCCCAA TCCTCCCCCT TGCTGTCCTG CCCCACCCCA
  121 CCCCCCAGAA TAGAATGACA CCTACTCAGA CAATGCGATG CAATTTCCTC ATTTTATTAG
  181 GAAAGGACAG TGGGAGTGGC ACCTTCCAGG GTCAAGGAAG GCACGGGGGA GGGGCAAACA
  241 ACAGATGGCT GGCAACTAGA AGGCACAGTC GAGGCTGATC AGCGGGTTTA AACGGGCCCT
  301 GCTAGAGATT TTCCACACTG ACTAAAAGGG TCTGAGGGAT CTCTAGTTAC CAGAGTCACA
  361 CAACAGACGG GCACACACTA CTTGAAGCAC TCAAGGCAAG CTTTATTGAG GCTTAAGCAG
  421 TGGGTTCCCT AGTTAGCCAG AGAGCTCCCA GGCTCAGATC TGGTCTAACC AGAGAGACCC
  481 AGTACAGTCC GGATGCAGCT CTCGGGCCAT GTGATGAAAT GCTAGGCGGC TGTCAAACCT
  541 CCACTCTAAT ACTTCTCTCT CCGGGTCATC CATCCCATGC AGGCTCACAG GGTGTAACAA
  601 GCGGGTGTTC TCTCCTTCAT TGGCTTCTTC TACCTTCTCT TGCTCAACTG GTACTAGCTT
  661 GTAGCACCAT CCAAAGGTCA GTGGATATCT GATCCCTGGC CCTGGTGTGT AGTTCTGCCN
  721 ATCAGGGAAG TAGCCTTGTG TGTGGTAGAT CCACAGATCA NGGATATCTT GTCTTCGTTG
  781 GNAGTGAATT AGCCCTTNNA GTCCCCCCTT TTCTTTTNAA AAGTNNNTAA GATCTACAGC
  841 TGCCTNNTAA GTCANTGNTC TTAAANNNAN CTGANGNGTG ANTNNAAAAC CCNCCTNNNN
  901 CTCNNNTTNN GCTTNTANNC NNGCNNNNTC AGCATNNNNN NCNGCTGNAN NNNTNNNNNN
961 GANNNNTNNN NNNTTTNNNN NNNNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 ori_pro, EM7 pro, BleoR, SV40 p(A)
Sequence file: RDB17498_A9Ioh.seq check
>D06551A9_A9Io_3_SV40pro_ori_F_G02_20_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNGCC GCCTCTGCCT CTGNNCTATT CNNGAAGTAG TGAGGAGGCT
   61 TTTTTGGAGG CCTAGGCTTT TGNNNAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT
  121 CTGATCAGCA CGTGTTGACA ATTAATCATC GGCATAGTAT ATCGGCATAG TATAATACGA
  181 CAAGGTGAGG AACTAAACCA TGGCCAAGTT GACCAGTGCC GTTCCGGTGC TCACCGCGCG
  241 CGACGTCGCC GGAGCGGTCG AGTTCTGGAC CGACCGGCTC GGGTTCTCCC GGGACTTCGT
  301 GGAGGACGAC TTCGCCGGTG TGGTCCGGGA CGACGTGACC CTGTTCATCA GCGCGGTCCA
  361 GGACCAGGTG GTGCCGGACA ACACCCTGGC CTGGGTGTGG GTGCGCGGCC TGGACGAGCT
  421 GTACGCCGAG TGGTCGGAGG TCGTGTCCAC GAACTTCCGG GACGCCTCCG GGCCGGCCAT
  481 GACCGAGATC GGCGAGCAGC CGTGGGGGCG GGAGTTCGCC CTGCGCGACC CGGCCGGCAA
  541 CTGCGTGCAC TTCGTGGCCG AGGAGCAGGA CTGACACGTG CTACGAGATT TCGATTCCAC
  601 CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT
  661 CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCCAACTTGT TTATTGCAGC
  721 TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC
  781 ACTGCATTCT AGTTGTGGTT TGTCCNAACT CATCAATGTA TCTTATCATG TCTGTATACC
  841 GTCGANCTCT ANCTAGAGCT TGGCGTAATC ATGGTCATAN CTGTTTCCTG TGTGAAATTG
  901 NTATCCGCTC ACAATTCCNC ACNANATACG ANCCNGAAGC ATAAAGTGTA AAGNNNGGGG
961 NGNCTAATGA NTGAGCNNAN NNNNATTNAT TGCGNNGCGN NNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Inano, S., RFWD3-Mediated Ubiquitination Promotes Timely Removal of Both RPA and RAD51 from DNA Damage Sites to Facilitate Homologous Recombination. Mol. Cell 66 (5): 622-634.e8 (2017). PMID 28575658. [link to RRC of NBRP]