Resource data sheet
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CSII FLAG-hRFWD3 wt

Lentivirus expression vector of human ring finger and WD repeat domain 3 (RFWD3) wild type.

Catalog number RDB17496
Resource name CSII FLAG-hRFWD3 wt
Clone info. Lentivirus expression vector of human ring finger and WD repeat domain 3 (RFWD3) wild type, tagged with FLAG at N-terminus, CMV promoter.
Expression was confirmed by the depositor with immunoblotting. Known differences (at least): G to A substitution at 1269nt of NM_018124.4 (R492K; AGG to AAG).
Vector backbone CSII-CMV-MCS-IRES-Bsd (plasmid)
Selectable markers Lentivirus transfection: Bsd^r. E. coli: Amp^r. Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Gene/insert name Human RFWD3 cDNA
Originator Takata, Minoru |
 
Other clones in our bank human RFWD3 (NCBI Gene 55159) |
Lentiviral plasmid list, maps and sequences information. [link]

Distribution information

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Inano, S et al. Mol. Cell, 66(5): 622-634, 2017)
Additional terms and conditions for distribution The RECIPIENT agrees to expressly describe Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose.
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RDB17496 CSII FLAG-hRFWD3 wt DNA solution

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Ordering instruction of plasmids [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Inano, S., RFWD3-Mediated Ubiquitination Promotes Timely Removal of Both RPA and RAD51 from DNA Damage Sites to Facilitate Homologous Recombination. Mol. Cell 66 (5): 622-634.e8 (2017). PMID 28575658.

Further references such as user reports and related articles (go to bottom)

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Sequence information

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


References

Original, user report and related articles

original Inano, S., RFWD3-Mediated Ubiquitination Promotes Timely Removal of Both RPA and RAD51 from DNA Damage Sites to Facilitate Homologous Recombination. Mol. Cell 66 (5): 622-634.e8 (2017). PMID 28575658.

2020.02.14

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