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CSII FLAG-hRFWD3 wt (#RDB17496)

Lentivirus expression vector of human ring finger and WD repeat domain 3 (RFWD3) wild type.

Clone info. Lentivirus expression vector of human ring finger and WD repeat domain 3 (RFWD3) wild type, tagged with FLAG at N-terminus, CMV promoter.
Comment Expression was confirmed by the depositor with immunoblotting. Known differences (at least): G to A substitution at 1269nt of NM_018124.4 (R392K; AGG to AAG).
Vector backbone CSII-CMV-MCS-IRES-Bsd (RDB04385) (plasmid)
Selectable markers Lentivirus transfection: Bsd^r. E. coli: Amp^r. Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Growth conditions LB+Ampicillin, 37oC
Gene/insert name Human RFWD3 cDNA
Depositor|Developer Takata, Minoru |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Ordering instruction.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Inano, S et al. Mol. Cell, 66(5): 622-634, 2017)
Additional terms and conditions:
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
Remarks
Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Inano, S et al. Mol. Cell, 66(5): 622-634, 2017)。
付加的使用条件：
本件研究材料は、非営利機関の非営利学術研究に限って提供する。レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
備考
Gateway®テクノロジーを用いた発現クローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17496 CSII FLAG-hRFWD3 wt DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge plus licensing fee equivalent for Gateway® expression clone

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The CSII FLAG-hRFWD3 wt was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17496).

Reference section:

Inano, S., Sato, K., Katsuki, Y., Kobayashi, W., Tanaka, H., Nakajima, K., Nakada, S., Miyoshi, H., Knies, K., Takaori-Kondo, A., Schindler, D., Ishiai, M., Kurumizaka, H., Takata, M., RFWD3-Mediated Ubiquitination Promotes Timely Removal of Both RPA and RAD51 from DNA Damage Sites to Facilitate Homologous Recombination. Mol. Cell 66 (5): 622-634.e8 (2017). PMID 28575658. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17496_A9Iop1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: hRFWD3-N_R (Pr0757)
Region: insert 5', attB1, FLAG, CMV pro 2, CMV en 2
Sequence file: RDB17496_A9Ioa.seq check
>D06551A7_A9Io_2_hRFWD3-N_R_G02_20_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN TNNGCAGGNN CTGGCTGGAN GNNGGATGGT
   61 ACCCCCTGGC TGCTGACCAC ATCAGCAGGA ACAGGCTGGA GGAGGGCTGG TCCCCCTTGG
  121 CTGCTGGCCA TGCCAGCAGG AGCTGGCTGT TGTTCGGCAT GATTTAACTG CACCTGAACA
  181 TCATATTCCA TTGCTTCATG AGCCATGGTG AAGGGGGCGG CCGCGGAGCC TGCTTTTTTG
  241 TACAAACTTG TTTGATAGCT TGGACCAGAA CCACCATGCT TATCGTCATC ATCTTTATAA
  301 TCCATGGTGC TAGCCAGCTT GGGTCTCCCT AGCGGATCTG ACGGTTCACT AAACCAGCTC
  361 TGCTTATATA GACCTCCCAC CGTACACGCC TACCGCCCAT TTGCGTCAAT GGGGCGGAGT
  421 TGTTACGACA TTTTGGAAAG TCCCGTTGAT TTTGGTGCCA AAACAAACTC CCATTGACGT
  481 CAATGGGGTG GAGACTTGGA AATCCCCGTG AGTCAAACCG CTATCCACGC CCATTGATGT
  541 ACTGCCAAAA CCGCATCACC ATGGTAATAG CGATGACTAA TACGTAGATG TACTGCCAAG
  601 TAGGAAAGTC CCATAAGGTC ATGTACTGGG CATAATGCCA GGCGGGCCAT TTACCGTCAT
  661 TGACGTCAAT AGGGGGCGTA CTTGGCATAT GATACACTTG ATGTACTGCC AAGTGGGCAG
  721 TTTACCGTAA ATACTCCACC CATTGACGTC AATGGAAAGT CCCTATTGGC GTTACTATGG
  781 GAACATACGT CATTATTGAC GTCAATGGGC GGGGGTCGTT GGGCGGTCAG CCAGGCGGGC
  841 CATTTACCGT AAGTTATGTA ACGCGGANNT CCNTATATGG GCTATGAACT AATGACCCCG
  901 TAATTGATTA CTATTAATCG AATTANNTCN AAGTGAATTT CTGCTGNCCN NGNNATAAAC
  961 CCNAAAATTT TGAATTTTTT GTNATTTGTT TTTGTAANTN CNTTANNTTG NATGNNNGNT
1021 NGNNNNNNNG NNNNNNANNN NTNNCCNNNN NNNNNN
//
Primer: IRES_R3 (Pr0573)
Region: attB2, insert 3'
Sequence file: RDB17496_A9Iob.seq check
>D06551A7_A9Io_2_IRES_R3_F03_18_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNCNNN NNNNGCGGNN NNNNGCAGAA
   61 TTCTCGATGG CAACTAGAAG GCACAGTCGA GGCTGATCAG CGGGTTTATC GAACCACTTT
  121 GTACNNNAAA GCTGGGTCGG CGCGCCCACC CTTTCACTCC CACTTATANA TGTGGACCAT
  181 CTTCTCTGTT AAGGTAGCCA AGTAGCTGTT ACGGTTCACC TCAAATGGGC AGATGTCCAA
  241 CACAGGCTGA TCGGTCTGTA GGTCCTGGAG CAACGAGCCA CTGGCAGCAT CCCACAGCAG
  301 GGCAGAATTT GCTGCTTCAT CCCCAGTACA CACCAGGATG TTGCCATCAT TCTCTGGGCT
  361 TTGGAAAATG GCATTTTTGG TCAATAGTTT GCAAGTAGGT CCTCCAAAAA ATGTATGTAC
  421 NGGCTGGCAG GAGCAGATTG GATTTCCAGT GTCATCCAGT CGGTAGGACA TTTCCATCAN
  481 CACACTTCGT ATGGTGGTGT GATTTTTATC AGGCCTGTAG GTCACAAGAC NNNGCCGGGA
  541 GCTGTTCTCT GTCTGAAAGT CTATGCAGCC CCCTGGCTCC AAGGGCAGNN NNNGAGGCCA
  601 ATGAGAAAAG TCCATTTTCT GTTCCCANAA TGAAGCATCC TNNNNNGTTC CAGCCAGCAC
  661 CNNNNNNTAT GNANNTGCNN CTGANGCAGC TCTGGGCATG TATGACAGGN ANACCAGTGG
  721 GCATCTGGCT TTCTGAGCTA CTAACTCCTG CACATGACTG CTCGTGTTTC GCACGTCATN
  781 NTACNNNAAN TTGAACCATT GGCCANNCCN NCATAGATGT AGTTNAGCCT CATCAAGACA
  841 CCAGCAACAG CTNNNGANNG GNANNNNNNN NCNTTATNAG TCTGGGANNN CNNGNNNTTT
  901 GNNTCCAGGC TGGNNCNNNT TTNANNANNN NNTGNCTAGG GAAANCANAA NAGTANNNNN
  961 CCTCTGANGN NANNNNNNNN AANNNNNNNT NCNNNGGATN NNNNNNGNCN NNNNNNCGGA
1021 ANNGNNNNNN NNNN
//
Primer: hRFWD3-N_F (Pr0753)
Region: insert mid
Sequence file: RDB17496_A9Ioc.seq check
>D06551A7_A9Io_2_hRFWD3-N_F_C01_07_ABI24.ab1
    1 NNNNNNNNNN NGNNNNNNNN NTCTGANAGT TGCAGGTGTC TAGTAGTTCT GATTCTGACA
   61 GTGACAGCTC TGCAGAGTAT GGAGGGGTTG TTGACCAGGC AGAGGAATCT GGAGCTGTCA
  121 TTTTAGAAGA GCAACTAGCA GGTGTCTCAG CAGAGCAAGA AGTTACATGT ATCGATGGAG
  181 GCAAGACCCT CCCCAAACAG CCATCTCCCC AGAAGTCTGA GCCTCTGCTA CCTTCTGCTT
  241 CTATGGATGA GGAAGAAGGG GACACTTGTA CAATATGTCT GGAACAGTGG ACCAATGCTG
  301 GGGACCACCG GCTCTCAGCA TTACGCTGTG GGCATCTCTT TGGGTATAGG TGCATTTCCA
  361 CGTGGCTTAA AGGACAAGTA CGAAAATGTC CCCAGTGCAA CAAGAAAGCC AGGCACAGTG
  421 ACATTGTCGT CCTTTATGCC CGAACCCTGA GAGCTTTGGA CACTAGTGAA CAGGAGCGCA
  481 TGAAAAGTTC CCTACTGAAG GAACAGATGC TAAGGAAACA GGCCGAGTTA GAATCAGCAC
  541 AGTGCCGACT CCAACTGCAG GTCCTCACTG ATAAGTGCAC TAAGCTTCAA AGGCGTGTTC
  601 AGGACTTGCA AAAACTTACG TCACATCAAA GTCAGAATTT ACAGCAACCC AGGGGCTCCC
  661 AAGCATGGGT CCTGAGCTGC TCACCCTCCA GCCAGGGCCA GCACAAGCAC AAGTACCACT
  721 TCCAAAAGAN CTTCACAGTA TCTCAGGCAG GAAACTGCCG GATCATGGCA TACTGTGATG
  781 CTCTGANCTG CCTGGTGATA TCACAGCCTT CTCCTCANGC CTCTTTTCTT CCAGGCTTTG
  841 GTGTTAAGAN GTTGANTACT GCCAACATGA AGAGCAGTCA GTACATTCCG ATGCATGGCA
  901 AACAGATNCG NGGANNNGCG TTTANCAGTT ANNNCANANG CTNGCTACTC TCTGCTTCCN
  961 NNGANANCNC TATNNAANNG ANNAGCCNNN NNANNAATNN CNNNNNNCNN ANNTNATNAN
1021 GNTGNNNNNN NNNN
//
Primer: HIVgp_R1 (Pr0132)
Region: HIV-1 psi, 5' LTR
Sequence file: RDB17496_A9Iod.seq check
>D06551A7_A9Io_2_HIVgp_R1_C01_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNGC TCTTTTTTCT CTCTGCACCN CTCTTCTCTT TGCCTTGGTG
   61 GGTGCTACTC CTAATGGTTC AATTTTTACT ACTTTATATT TATATAATTC ACTTCTCCAA
  121 TTGTCCCTCA TATCTCCTCC TCCAGGTCTG AAGATCAGCG GCCGGCCGCT TGCTGTGCGG
  181 TGGTCTTACT TTTGTTTTGC TCTTCCTCTA TCTTGTCTAA AGCTTCCTTG GTGTCTTTTA
  241 TCTCTATCCT TTGATGCACA CAATAGAGGG TTGCTACTGT ATTATATAAT GATCTAAGTT
  301 CTTCTGATCC TGTCTGAAGG GATGGTTGTA GCTGTCCCAG TATTTGTCTA CAGCCTTCTG
  361 ATGTTTCTAA CAGGCCAGGA TTAACTGCGA ATCGTTCTAG CTCCCTGCTT GCCCATACTA
  421 TATGTTTTAA TTTATATTTT TTCTTTCCCC CTGGCCTTAA CCGAATTTTT TCCCATCGCG
  481 ATCTAATTCT CCCCCGCTTA ATACTGACGC TCTCGCACCC ATCTCTCTCC TTCTAGCCTC
  541 CGCTAGTCAA AATTTTTGGC GTACTCACCA GTCGCCGCCC CTCGCCTCTT GCCGTGCGCG
  601 CTTCAGCAAG CCGAGTCCTG CGTCGAGAGA GCTCCTCTGG TTTCCCTTTC GCTTTCAAGT
  661 CCCTGTTCGG GCGCCACTGC TAGAGATTTT CCACACTGAC TAAAAGGGTC TGANGGATCT
  721 CTAGTTACCA GAGTCACACA ACAGACGGGC ACACACTACT TGAAGCACTC AAGGCAAGCT
  781 TTATTGAGGC TTAAGCAGTG GGTTCCCTAG TTAGCCAGAG AGCTCCCAGG CTCAGATCTG
  841 GTCTAACCAG ANAGACCCAG TACAGGCAAA ANGCGCTGCT TATATAGANN TCCCACCGNA
  901 CACGCCTANC GCCCATTTGC GNNAATGGGG CGGANTNGTT NCNANNTTTT GNAAAGTCCC
961 GTTGANTTTG GTGNCAAANN AAANNNCCNT TGANNNNNNN GGGGNNNNNN NNNN
//
Primer: cPPT+CTS_v2_F (Pr0303)
Region: cPPT_CTS, CMV en 2, CMV pro 2
Sequence file: RDB17496_A9Ioe.seq check
>D06551A7_A9Io_2_cPPT+CTS_v2_F_D01_10_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNACNA NNACNAATTA CAAAAATTCA AAATTTTCGG
   61 GTTTATTACA GGGACAGCAG AAATTCACTT TGAATTAATT CGATTAATAG TAATCAATTA
  121 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG
  181 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC
  241 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA
  301 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA
  361 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA
  421 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT
  481 ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG
  541 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA
  601 ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA
  661 GAGCTGGTTT AGTGAACCGT CNNATCCGCT AGGGAGACCC AAGCTGGCTA GCACCATGGA
  721 TTATAAAGAT GATGACGATA AGCATGGTGG TTCTGGTCCA AGCTATCAAA CAAGTTTGTA
  781 CAAAAAAGCA NGCTCCGCGG CCGCCCCCTT CACNATGGCT CATGAANCNN NGNAATATGA
  841 NGTTCANGTG CAGTTAANTC ATGCCNAACA ACANCCAGCT NCTGCTGGCA NGGNCAGCAG
  901 CNNNGGGGGN NCANCCCTCC TNCANCCTGN NNCNGNTGAT GTGNNCAGCA GCCAGGGGGN
  961 ACNNTNNNTC CNNNNNCNNN NNNNNGCNGN NNNATNANCA GNCNNANCGN NANNACCNNN
1021 NNNNNNN
//
Primer: IRES-F (Pr0036)
Region: IRES2, BSD, WPRE
Sequence file: RDB17496_A9Iof.seq check
>D06551A7_A9Io_2_IRES-F_F01_16_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNTNN GTCNANGTTN NAAAAACGTC TAGGCCCCCC GAACCACGGG
   61 GACGTGGTTT TCCTTTGAAA AACACGATGA TAATATGGCC ACAACCATGG CCAAGCCTTT
  121 GTCTCAAGAA GAATCCACCC TCATTGAAAG AGCAACGGCT ACAATCAACA GCATCCCCAT
  181 CTCTGAAGAC TACAGCGTCG CCAGCGCAGC TCTCTCTAGC GACGGCCGCA TCTTCACTGG
  241 TGTCAATGTA TATCATTTTA CTGGGGGACC TTGTGCAGAA CTCGTGGTGC TGGGCACTGC
  301 TGCTGCTGCG GCAGCTGGCA ACCTGACTTG TATCGTCGCG ATCGGAAATG AGAACAGGGG
  361 CATCTTGAGC CCCTGCGGAC GGTGCCGACA GGTGCTTCTC GATCTGCATC CTGGGATCAA
  421 AGCCATAGTG AAGGACAGTG ATGGACAGCC GACGGCAGTT GGGATTCGTG AATTGCTGCC
  481 CTCTGGTTAT GTGTGGGAGG GCTAAGTCGA GCCTCTAGAG TTAACATCGA GGGATCAAGC
  541 TTATCGATAA TCAACCTCTG GATTACAAAA TTTGTGAAAG ATTGACTGGT ATTCTTAACT
  601 ATGTTGCTCC TTTTACGCTA TGTGGATACG CTGCTTTAAT GCCTTTGTAT CATGCTATTG
  661 CTTCCCGTAT GGCTTTCATT TTCTCCTCCT TGTATAAATC CTGGTTGCTG TCTCTTTATG
  721 AGGAGTTGTG GCCCGTTGTC AGGCAACGTG GCGTGGTGTG CACTGTGTTT GCTGACGCAA
  781 CCCCCACTGG TTGGGGCATT GCCACCACCT GTCAGCTCCT TTCCGGGANT TTCGCTTTCC
  841 CCCTNNNTAT TGCCACGGCG GAACTCATCG CCGCCTGCNT TGNCCGCTGC TGGANAGGGG
  901 CTCGGCTGTT NGGCACTGAC NATNNCGNNN NGNTGTCGGG GAAGCTGANG TCCTTTCCAN
  961 GGNTGCTCNN CTGNGNNGNC NCCNNNNNTC TGNGCGGGNN GNCCNTNNNG NNNCNNNCNT
1021 NNNGNNCNNN NNTNNNNCNN ANNNTNNNNT NNNNNNNGNN NNNNNNNN
//
Primer: BGH_rev2 (Pr0606)
Region: bGH p(A), 3' LTR, WPRE
Sequence file: RDB17496_A9Iog.seq check
>D06551A7_A9Io_2_BGH_rev2_E01_13_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNG GGGGNNGGGC AAACNACAGA TGGCTGGCAA CTAGAAGGCA
   61 CAGTCGAGGC TGATCAGCGG GTTTAAACGG GCCCTGCTAG AGATTTTCCA CACTGACTAA
  121 AAGGGTCTGA GGGATCTCTA GTTACCAGAG TCACACAACA GACGGGCACA CACTACTTGA
  181 AGCACTCAAG GCAAGCTTTA TTGAGGCTTA AGCAGTGGGT TCCCTAGTTA GCCAGAGAGC
  241 TCCCAGGCTC AGATCTGGTC TAACCAGAGA GACCCAGTAC AGTCCGGATG CAGCTCTCGG
  301 GCCATGTGAT GAAATGCTAG GCGGCTGTCA AACCTCCACT CTAATACTTC TCTCTCCGGG
  361 TCATCCATCC CATGCAGGCT CACAGGGTGT AACAAGCGGG TGTTCTCTCC TTCATTGGCT
  421 TCTTCTACCT TCTCTTGCTC AACTGGTACT AGCTTGTAGC ACCATCCAAA GGTCAGTGGA
  481 TATCTGATCC CTGGCCCTGG TGTGTAGTTC TGCCAATCAG GGAAGTAGCC TTGTGTGTGG
  541 TAGATCCACA GATCAAGGAT ATCTTGTCTT CGTTGGGAGT GAATTAGCCC TTCCAGTCCC
  601 CCCTTTTCTT TTAAAAAGTG GCTAAGATCT ACAGCTGCCT TGTAAGTCAT TGGTCTTAAA
  661 GGTACCTGAG GTGTGACTGG AAAACCCACC TCCTCCTCCT CTTGTGCTTC TAGCCAGGCA
  721 CAATCAGCAT TGGTAGCTGC TGTATTGCTA CTTGTGATTG CTCCATGTTT TTCTAGGTCT
  781 CGACGGTATC GATGCGGGGA NGCGGCCCAA AGGGAGATCC GANTCGTCTG ANGGCGAANG
  841 CGAAGANGCG GAAGANGNCG CAGANCNNGN AGCANGNCGC GGGAANGAAN GTCCGCTNGA
  901 NTGAGGGNCG AANGGANGTA NCANANNNNA NGTCCCGCGC ANAATCCNNN TGGNNNCNCA
  961 GGNGANCANC CANNGNAANN NNNGNCANCT TNCNNGANNN NNNNNNGNNN NNGNCNNNNN
1021 NNNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori, EM7 pro, BleoR
Sequence file: RDB17496_A9Ioh.seq check
>D06551A7_A9Io_2_SV40pro_ori_F_G01_19_ABI24.ab1
    1 NNNNNNNNNN NNNGNNGNCN CCTCTGCCTC TGANCTATTC NNGAAGTAGT GAGGAGGCTT
   61 TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC
  121 TGATCAGCAC GTGTTGACAA TTAATCATCG GCATAGTATA TCGGCATAGT ATAATACGAC
  181 AAGGTGAGGA ACTAAACCAT GGCCAAGTTG ACCAGTGCCG TTCCGGTGCT CACCGCGCGC
  241 GACGTCGCCG GAGCGGTCGA GTTCTGGACC GACCGGCTCG GGTTCTCCCG GGACTTCGTG
  301 GAGGACGACT TCGCCGGTGT GGTCCGGGAC GACGTGACCC TGTTCATCAG CGCGGTCCAG
  361 GACCAGGTGG TGCCGGACAA CACCCTGGCC TGGGTGTGGG TGCGCGGCCT GGACGAGCTG
  421 TACGCCGAGT GGTCGGAGGT CGTGTCCACG AACTTCCGGG ACGCCTCCGG GCCGGCCATG
  481 ACCGAGATCG GCGAGCAGCC GTGGGGGCGG GAGTTCGCCC TGCGCGACCC GGCCGGCAAC
  541 TGCGTGCACT TCGTGGCCGA GGAGCAGGAC TGACACGTGC TACGAGATTT CGATTCCACC
  601 GCCGCCTTCT ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC
  661 CTCCAGCGCG GGGATCTCAT GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT
  721 TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA
  781 CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT CTGTATACCG
  841 TCGANCTCTA NCTAGAGCTT GNCGTAATCN TGGNCATAGC TGTTTCCTGT GTGAAATTGT
  901 TATCCGCTCA CNATTCCACA CNACATACNA NCNNGAAGCA TAAAGNGTAA ANNNNGGGGG
  961 NGNCTNNNGA NTGANCTNNC TNNNATTNNN TNGNNNNNNN NNCACNGNCN NNNTTNCNNN
1021 NNGNNANNNN NNNNNGNNNN NNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Inano, S., RFWD3-Mediated Ubiquitination Promotes Timely Removal of Both RPA and RAD51 from DNA Damage Sites to Facilitate Homologous Recombination. Mol. Cell 66 (5): 622-634.e8 (2017). PMID 28575658. [link to RRC of NBRP]