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pcDNA3.1 FLAG-hRFWD3 LQP/AAA

Expression vector of human ring finger and WD repeat domain 3 (RFWD3) mutant, LQP/AAA, LQP motifs replaced by triple alanines.

Catalog number RDB17495
Resource name pcDNA3.1 FLAG-hRFWD3 LQP/AAA
Clone info. Expression vector of human ring finger and WD repeat domain 3 (RFWD3) mutant, LQP/AAA, LQP motifs replaced by triple alanines, tagged with FLAG at N-terminus, see Fig. S5B of Inano, S. et al., Mol. Cell, 66 (5): 622-634, 2017.
Comment Expression was confirmed by the depositor with immunoblotting. Known differences (at least): G to A substitution at 1269nt of NM_018124.4 (R392K; AGG to AAG).
Vector backbone pcDNA3.1/Zeo (plasmid)
Selectable markers E. coli, Ampicillin; mammalian cells, Zeocin.
Gene/insert name Human RFWD3 cDNA
Depositor|Developer Takata, Minoru |
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External Database
Human RFWD3

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Inano, S et al. Mol. Cell, 66(5): 622-634, 2017)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Inano, S et al. Mol. Cell, 66(5): 622-634, 2017)。
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          RDB17495 pcDNA3.1 FLAG-hRFWD3 LQP/AAA DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Inano, S., RFWD3-Mediated Ubiquitination Promotes Timely Removal of Both RPA and RAD51 from DNA Damage Sites to Facilitate Homologous Recombination. Mol. Cell 66 (5): 622-634.e8 (2017). PMID 28575658.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB17495_A9Iop1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward
          Region: CMV pro, FLAG, attB1, insert 5' (LQP/AAA)
          Sequence file: RDB17495_A9Ioa.seq check
          >D06551A6_A9Io_1_CMV_Forward_E02_14_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNGCN NNTGGGCGGT AGGCGTGTAC GGTGGGAGGT
             61 CTATATAAGC AGAGCTCTCT GGCTAACTAG AGAACCCACT GCTTACTGGC TTATCGAAAT
            121 TAATACGACT CACTATAGGG AGACCCAAGC TGGCTAGCAC CATGGATTAT AAAGATGATG
            181 ACGATAAGCA TGGTGGTTCT GGTCCAAGCT ATCAAACAAG TTTGTACAAA AAAGCAGGCT
            241 CCGCGGCCGC CCCCTTCACC ATGGCTCATG AAGCAATGGA ATATGATGTT CAGGTGCAGT
            301 TAAATCATGC CGAAGCAGCT GCAGCTCCTG CTGGCATGGC CAGCAGCCAA GGGGGACCAG
            361 CCCTCGCTGC AGCTGTTCCT GCTGATGTGG TCAGCAGCCA GGGGGTACCA TCCATCGCAG
            421 CTGCAGCTCC TGCTGAGGTG ATCAGCAGCC AAGCGACACC ACCCCTGGCA GCAGCTGCTC
            481 CGCAACTGTC TGTTGACCTG ACAGAAGTGG AGGTCTTGGG AGAAGACACT GTGGAGAACA
            541 TCAATCCAAG AACTTCAGAA CAACATAGGC AGGGATCTGA TGGTAATCAC ACCATCCCAG
            601 CATCTTCGTT GCATTCAATG ACCAACTTCA TCAGCGGACT GCAGAGACTT CATGGCATGC
            661 TGGAATTCCT GAGACCTTCA TCTTCAAACC ACAGTGTAGG GCCAATGAGA ACAAGAAGGA
            721 GGGTATCTGC TTCACGGAGG GCAAGANCCG GAGGGTCTCA GANGACANAC AGTGCCAGGT
            781 TGAGAGCACC ATTGGATGCT TACTTTCNGG TGAGCAGGAN CCAGCCTGAC TTGCCNGCTA
            841 CCACTTATGA TTNNNANACT AGGAATCCTG TATCTGAAGA GTTGCNGGTG TCTAGTANTT
            901 CTGATTCTGA NNGTGACAGC TCTGCANANT ATGGANGGGT TGTTGNNNNN GNNNNAGGAA
            961 TCNNGANCTG NCNTTTTANA AGANNNACTN GNNNNGTGNN TCANNNNNNC NANNAANTTA
          1021 CNNNNNNNNN TGGNNNNNNN NNN
          //
          Primer: BGH_rev2
          Region: bGH p(A), attB2, insert 3'
          Sequence file: RDB17495_A9Iob.seq check
          >D06551A6_A9Io_1_BGH_rev2_A03_03_ABI24.ab1
              1 NNNNNNNNNN NNNNNGNNNG GGNGNNGGGC AAACNACAGA TGGCTGGCAA CTAGAAGGCA
             61 CAGTCGAGGC TGATCAGCGG GTTTATCGAA CCACTTTGTA CAAGAAAGCT GGGTCGGCGC
            121 GCCCACCCTT TCACTCCCAC TTATAGATGT GGACCATCTT CTCTGTTAAG GTAGCCAAGT
            181 AGCTGTTACG GTTCACCTCA AATGGGCAGA TGTCCAACAC AGGCTGATCG GTCTGTAGGT
            241 CCTGGAGCAA CGAGCCACTG GCAGCATCCC ACAGCAGGGC AGAATTTGCT GCTTCATCCC
            301 CAGTACACAC CAGGATGTTG CCATCATTCT CTGGGCTTTG GAAAATGGCA TTTTTGGTCA
            361 ATAGTTTGCA AGTAGGTCCT CCAAAAAATG TATGTACAGG CTGGCAGGAG CAGATTGGAT
            421 TTCCAGTGTC ATCCAGTCGG TAGGACATTT CCATCAGCAC ACTTCGTATG GTGGTGTGAT
            481 TTTTATCAGG CCTGTAGGTC ACAAGACAGT GCCGGGAGCT GTTCTCTGTC TGAAAGTCTA
            541 TGCAGCCCCC TGGCTCCAAG GGCAGCACAT GAGGCCAATG AGAAAAGTCC ATTTTCTGTT
            601 CCCAGAATGA AGCATCCTCC AAGGTTCCAG CCAGCACCCC ACCATATGGA AATGCAGCTG
            661 AGGCAGCTCT GGGCATGTAT GACAGGGAGA CCAGTGGGCA TCTGGCTTTC TGAGCTACTA
            721 ACTCCTGCAC ATGACTGCTC GTGTTTCGCA CGTCATATAC CAGAATTGAA CCATTGGCCA
            781 GTCCAGCATA GATGTAGTTA GCCTCATCAA GACACCAGCA ACAGCTCCNG ANAGGACGTC
            841 CAGCATTATA AGTCTGGANC ACGGNATTTG TCTCCAGGCT GGTCANTTTA ATAGNGTTGT
            901 CNANGGAANC ANANANTANC AAGCCTCTGA NGTAANTGCT AANNGCCANT NNNNNGNATC
            961 NGNTTNNCNT GCNTCNGANN GNNCTGACTG NNNNNCNTGN TNGNNAGTAN NNNNNATNNN
          1021 NNNNNNN
          //
          Primer: hRFWD3-N_F
          Region: insert mid
          Sequence file: RDB17495_A9Ioc.seq check
          >D06551A6_A9Io_1_hRFWD3-N_F_B01_04_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNTC TGANANTTGC AGGTGTCTAG TAGTTCTGAT TCTGACAGTG
             61 ACAGCTCTGC AGAGTATGGA GGGGTTGTTG ACCAGGCAGA GGAATCTGGA GCTGTCATTT
            121 TAGAAGAGCA ACTAGCAGGT GTCTCAGCAG AGCAAGAAGT TACATGTATC GATGGAGGCA
            181 AGACCCTCCC CAAACAGCCA TCTCCCCAGA AGTCTGAGCC TCTGCTACCT TCTGCTTCTA
            241 TGGATGAGGA AGAAGGGGAC ACTTGTACAA TATGTCTGGA ACAGTGGACC AATGCTGGGG
            301 ACCACCGGCT CTCAGCATTA CGCTGTGGGC ATCTCTTTGG GTATAGGTGC ATTTCCACGT
            361 GGCTTAAAGG ACAAGTACGA AAATGTCCCC AGTGCAACAA GAAAGCCAGG CACAGTGACA
            421 TTGTCGTCCT TTATGCCCGA ACCCTGAGAG CTTTGGACAC TAGTGAACAG GAGCGCATGA
            481 AAAGTTCCCT ACTGAAGGAA CAGATGCTAA GGAAACAGGC CGAGTTAGAA TCAGCACAGT
            541 GCCGACTCCA ACTGCAGGTC CTCACTGATA AGTGCACTAA GCTTCAAAGG CGTGTTCAGG
            601 ACTTGCAAAA ACTTACGTCA CATCAAAGTC AGAATTTACA GCAACCCAGG GGCTCCCAAG
            661 CATGGGTCCT GAGCTGCTCA CCCTCCAGCC AGGGCCAGCA CAAGCACAAG TACCACTTCC
            721 AAAAGANCTT CACAGTATCT CAGGCAGGAA ACTGCCGGAT CATGGCATAC TGTGNTGCTC
            781 TGAGCTGCCT GGTGATATCA CAGCCTTCTC CTCANGCCTC TTTTCTTCCN GGCTTTNNTG
            841 TTAAGANGTT GAGTACTGCN NACATGAAGA NCANNCAGTA CATTCNGATG NATGGCAAAC
            901 AGATNCGNGG NNNGGCGTTT ANCANTTACN TCAGANGCTT GCNACTNTCN GCTTCCNNAN
          961 NNNACACTAT NAANNNGANN NGNCNNNNNN NNNANTANNN NNNNNNN
          //
          Primer: SV40pro_ori_F
          Region: SV40 pro_ori, bleoR
          Sequence file: RDB17495_A9Iod.seq check
          >D06551A6_A9Io_1_SV40pro_ori_F_B03_06_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNCNCTGCC TCTGAGCNNT TCNNGAAGTA GTGAGGAGGC
             61 TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC CATTTTCGGA
            121 TCTGATCAGC ACGTGTTGAC AATTAATCAT CGGCATAGTA TATCGGCATA GTATAATACG
            181 ACAAGGTGAG GAACTAAACC ATGGCCAAGT TGACCAGTGC CGTTCCGGTG CTCACCGCGC
            241 GCGACGTCGC CGGAGCGGTC GAGTTCTGGA CCGACCGGCT CGGGTTCTCC CGGGACTTCG
            301 TGGAGGACGA CTTCGCCGGT GTGGTCCGGG ACGACGTGAC CCTGTTCATC AGCGCGGTCC
            361 AGGACCAGGT GGTGCCGGAC AACACCCTGG CCTGGGTGTG GGTGCGCGGC CTGGACGAGC
            421 TGTACGCCGA GTGGTCGGAG GTCGTGTCCA CGAACTTCCG GGACGCCTCC GGGCCGGCCA
            481 TGACCGAGAT CGGCGAGCAG CCGTGGGGGC GGGAGTTCGC CCTGCGCGAC CCGGCCGGCA
            541 ACTGCGTGCA CTTCGTGGCC GAGGAGCAGG ACTGACACGT GCTACGAGAT TTCNATTCCN
            601 CCGCCGCCTT CTATGAAAGG NTGGGCTTCG NANTCNTTTT CCGGGACGCC GGCTGNATGA
            661 TCCTCCNGCG CNGGGATCTC ATGCTGGANT NCTNCGCCCN CCCNNACTTG NTTATTGNAG
            721 CTTATAATGG TTANAANTNA AAGCAATAGC ATCNCAAATT TCNNNANNAA AGCNNTTTTT
            781 TNNCTGNNTN CNNNTTGTGG NTTGNNNNAN CTCNTCNNNG NANNNNNNNC NNGNNNNGNN
          841 NNNNCGNCNA CNTCNNNNTN NNNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Inano, S., RFWD3-Mediated Ubiquitination Promotes Timely Removal of Both RPA and RAD51 from DNA Damage Sites to Facilitate Homologous Recombination. Mol. Cell 66 (5): 622-634.e8 (2017). PMID 28575658.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl