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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

HELLS px330 (#RDB17381)

Expression vector of guide RNA for targeting human HELLS exon 19.

Alternative name HELLS exon19 pX330-U6-Chimeric_BB-CBh-hSpCas9
Clone info. Expression vector of guide RNA for targeting human helicase lymphoid specific (HELLS) exon 19 (5'-GAACCCCCAGTCGGATCTTC-3') and human codon-optimized SpCas9.
Vector backbone pX330-U6-Chimeric_BB-CBh-hSpCas9 (plasmid)
Size of vector backbone 8.5 kb
Selectable markers Amp^r (E. coli)
Gene/insert name human HELLS dsOligo
Depositor|Developer Unoki, Motoko |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Unoki, M et al. J. Clin. Invest. 129 (1), 78-92, 2019) and an acknowledgment to the DEPOSITOR are requested.
Remarks
The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.
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提供依頼書  [依頼書の記入例 ]
専用MTA（CRISPR/Cas9内包遺伝子材料専用 非営利学術目的）をお使いください [Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Unoki, M et al. J. Clin. Invest. 129 (1), 78-92, 2019) を引用し、謝辞の表明を必要とする。
備考
本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

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RDB17381 HELLS px330 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The HELLS px330 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17381).

Reference section:

Unoki, M., Funabiki, H., Velasco, G., Francastel, C., Sasaki, H., CDCA7 and HELLS mutations undermine nonhomologous end joining in centromeric instability syndrome. J. Clin. Invest. 129 (1): 78-92 (2019). PMID 30307408. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17381_A9Fhp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: SV40pA3'element reverse (Pr0108)
Region: U6 pro, gRNA (HELLS) gRNA scaffold, CBh pro 5'
Sequence file: RDB17381_A9Fha.seq check
>D06399A8_A9Fh_2_SV40pA3'element reverse_E03_15_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNCNNGCAAC GCGGCCTTTT TACGGTTCCT
   61 GGCCTTTTGC TGGCCTTTTG CTCACATGTG AGGGCCTATT TCCCATGATT CCTTCATATT
  121 TGCATATACG ATACAAGGCT GTTAGAGAGA TAATTGGAAT TAATTTGACT GTAAACACAA
  181 AGATATTAGT ACAAAATACG TGACGTAGAA AGTAATAATT TCTTGGGTAG TTTGCAGTTT
  241 TAAAATTATG TTTTAAAATG GACTATCATA TGCTTACCGT AACTTGAAAG TATTTCGATT
  301 TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAAACACCGA ACCCCCAGTC GGATCTTCGT
  361 TTTAGAGCTA GAAATAGCAA GTTAAAATAA GGCTAGTCCG TTATCAACTT GAAAAAGTGG
  421 CACCGAGTCG GTGCTTTTTT GTTTTAGAGC TAGAAATAGC AAGTTAAAAT AAGGCTAGTC
  481 CGTTTTTAGC GCGTGCGCCA ATTCTGCAGA CAAATGGCTC TAGAGGTACC CGTTACATAA
  541 CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA
  601 GTAACGCCAA TAGGGACTTT CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC
  661 CACTTGGCAG TACATCAAGT GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC
  721 GGTAAATGGC CCGCCTGGCA TTGTGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG
  781 CAGTACATCT ACGTATTAGT CATCGCTATT ACCATGGTCG AGGTGAGCCC CACGTTCTGC
  841 TTCACTCTCC CCATCTCCCC CCCCCTCCCC ACCCCCAANT TTGTATTTAT TTATTTTTTT
901 NATTATTTTG TGCANCGATN GGGGGCGGGG GGGGGGGGGG GGGNN
//
Primer: Cas9-N_R1 (Pr0650)
Region: Cas9 5', SV40 NLS, 3xFLAG, CBh pro 3'
Sequence file: RDB17381_A9Fhb.seq check
>D06399A8_A9Fh_2_Cas9-N_R1_D02_11_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNTGNANT CGTCGGTGNN CACGGCCCNG CCCACAGAGT
   61 TGGTGCCGAT GTCCAGGCCG ATGCTGTACT TCTTGTCGGC TGCTGGGACT CCGTGGATAC
  121 CGACCTTCCG CTTCTTCTTT GGGGCCATCT TATCGTCATC GTCTTTGTAA TCAATATCAT
  181 GATCCTTGTA GTCTCCGTCG TGGTCCTTAT AGTCCATGGT GGCACCGGTC CAACCTGAAA
  241 AAAAGTGATT TCAGGCAGGT GCTCCAGGTA ATTAAACATT AATACCCCAC CAACCAACCA
  301 TCCCTTAAAC CCTTACCTCT TGCTCAGCTA ATTACAGCCC GGAGGAGAAG GGCCGTCCCG
361 CCCGCTCACC TGTGGGAGTA ACGCGGTCAG TCAGAGCCGG GGCGGGCGGC GCGAGGNN
//
Primer: BGH_rev2 (Pr0606)
Region: bGH p(A), nucleoplasmin NLS, Cas9 3'
Sequence file: RDB17381_A9Fhc.seq check
>D06399A8_A9Fh_2_BGH_rev2_E02_05_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NGGGGGNNGG GCNNACNACA GNNGGCTGGC AACTAGAAGG
   61 CACAGTCGAN GCTGATCAGC GAGCTCTAGG AATTCTTACT TTTTCTTTTT TGCCTGGCCG
  121 GCCTTTTTCG TGGCCGCCGG CCTTTTGTCG CCTCCCAGCT GAGACAGGTC GATCCGTGTC
  181 TCGTACAGGC CGGTGATGCT CTGGTGGATC AGGGTGGCGT CCAGCACCTC TTTGGTGCTG
  241 GTGTACCTCT TCCGGTCGAT GGTGGTGTCA AAGTACTTGA AGGCGGCAGG GGCTCCCAGA
  301 TTGGTCAGGG TAAACAGGTG GATGATATTC TCGGCCTGCT CTCTGATGGG CTTATCCCGG
  361 TGCTTGTTGT AGGCGGACAG CACTTTGTCC AGATTAGCGT CGGCCAGGAT CACTCTCTTG
  421 GAGAACTCGC TGATCTGCTC GATGATCTCG TCCAGGTAGT GCTTGTGCTG TTCCACAAAC
  481 AGCTGTTTCT GCTCATTATC CTCGGGGGAG CCCTTCAGCT TCTCATAGTG GCTGGCCAGG
  541 TACAGGAAGT TCACATATTT GGAGGGCAGG GCCAGTTCGT TTCCCTTCTG CAGTTCGCCG
  601 GCAGAGGCCA GCATTCTCTT CCGGCCGTTT TCCAGCTCGA ACAGGGAGTA CTTAGGCAGC
  661 TTGATGATCA GGTCCTTTTT CACTTCTTTG TAGCCCTTGG CTTCCAGAAA GTCGATGGGA
  721 NTCTTCTCGA AGCTGCTTCT TTCCATGATG GTGATCCCCA GCAGCTCTTT CACACTCTTC
  781 AGTTTCTTGG ACTTGCCCTT TTCCACTTTG GCCACCACCA GCACAGAATA GGCCACGGNN
  841 GGGCTGTCGA ANCCGCCGTA CTTCTNANGG TCCCANNCCN TCTTTCTGGC GATCAGCTTA
901 TCGCNNTNCC NNTNGGGNAG GANANANTCN TNGCTGNNNN NNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Unoki, M., CDCA7 and HELLS mutations undermine nonhomologous end joining in centromeric instability syndrome. J. Clin. Invest. 129 (1): 78-92 (2019). PMID 30307408. [link to RRC of NBRP]