icon below as well as clone information before placing your order.
Expression vector of guide RNA for targeting human HELLS exon 19.
| Catalog number | RDB17381 |
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| Resource name | HELLS px330 |
| Alternative name | HELLS exon19 pX330-U6-Chimeric_BB-CBh-hSpCas9 |
| Clone info. | Expression vector of guide RNA for targeting human helicase lymphoid specific (HELLS) exon 19 (5'-GAACCCCCAGTCGGATCTTC-3') and human codon-optimized SpCas9. |
| Vector backbone | pX330-U6-Chimeric_BB-CBh-hSpCas9 (plasmid) |
| Size of vector backbone | 8.5 kb |
| Selectable markers | Amp^r (E. coli) |
| Gene/insert name | human HELLS dsOligo |
| Depositor|Developer | Unoki, Motoko | |
| Other clones in our bank | human HELLS (NCBI Gene 3070) | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Unoki, M et al. J. Clin. Invest. 129 (1), 78-92, 2019) and an acknowledgment to the DEPOSITOR are requested. |
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| Ordering | Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment  ] [Bank Transfer or Check Payment]Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word ] |
| Remarks | The BIOLOGICAL RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations. Please refer the Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose). |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB17381 | HELLS px330 | DNA solution |
icon below as well as clone information before placing your order.Electronic file
Original reference
| original | Unoki, M., CDCA7 and HELLS mutations undermine nonhomologous end joining in centromeric instability syndrome. J. Clin. Invest. 129 (1): 78-92 (2019). PMID 30307408. |
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Further references such as user reports and related articles (go to bottom)
Featured content
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
| Test sheet | RDB17381_A9Fhp1-1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
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Primer: SV40pA3'element reverse Region: U6 pro, gRNA (HELLS) gRNA scaffold, CBh pro 5' Sequence file: RDB17381_A9Fha.seq
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>D06399A8_A9Fh_2_SV40pA3'element reverse_E03_15_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNCNNGCAAC GCGGCCTTTT TACGGTTCCT 61 GGCCTTTTGC TGGCCTTTTG CTCACATGTG AGGGCCTATT TCCCATGATT CCTTCATATT 121 TGCATATACG ATACAAGGCT GTTAGAGAGA TAATTGGAAT TAATTTGACT GTAAACACAA 181 AGATATTAGT ACAAAATACG TGACGTAGAA AGTAATAATT TCTTGGGTAG TTTGCAGTTT 241 TAAAATTATG TTTTAAAATG GACTATCATA TGCTTACCGT AACTTGAAAG TATTTCGATT 301 TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAAACACCGA ACCCCCAGTC GGATCTTCGT 361 TTTAGAGCTA GAAATAGCAA GTTAAAATAA GGCTAGTCCG TTATCAACTT GAAAAAGTGG 421 CACCGAGTCG GTGCTTTTTT GTTTTAGAGC TAGAAATAGC AAGTTAAAAT AAGGCTAGTC 481 CGTTTTTAGC GCGTGCGCCA ATTCTGCAGA CAAATGGCTC TAGAGGTACC CGTTACATAA 541 CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA 601 GTAACGCCAA TAGGGACTTT CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC 661 CACTTGGCAG TACATCAAGT GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC 721 GGTAAATGGC CCGCCTGGCA TTGTGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG 781 CAGTACATCT ACGTATTAGT CATCGCTATT ACCATGGTCG AGGTGAGCCC CACGTTCTGC 841 TTCACTCTCC CCATCTCCCC CCCCCTCCCC ACCCCCAANT TTGTATTTAT TTATTTTTTT 901 NATTATTTTG TGCANCGATN GGGGGCGGGG GGGGGGGGGG GGGNN // |
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Primer: Cas9-N_R1 Region: Cas9 5', SV40 NLS, 3xFLAG, CBh pro 3' Sequence file: RDB17381_A9Fhb.seq
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>D06399A8_A9Fh_2_Cas9-N_R1_D02_11_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNTGNANT CGTCGGTGNN CACGGCCCNG CCCACAGAGT 61 TGGTGCCGAT GTCCAGGCCG ATGCTGTACT TCTTGTCGGC TGCTGGGACT CCGTGGATAC 121 CGACCTTCCG CTTCTTCTTT GGGGCCATCT TATCGTCATC GTCTTTGTAA TCAATATCAT 181 GATCCTTGTA GTCTCCGTCG TGGTCCTTAT AGTCCATGGT GGCACCGGTC CAACCTGAAA 241 AAAAGTGATT TCAGGCAGGT GCTCCAGGTA ATTAAACATT AATACCCCAC CAACCAACCA 301 TCCCTTAAAC CCTTACCTCT TGCTCAGCTA ATTACAGCCC GGAGGAGAAG GGCCGTCCCG 361 CCCGCTCACC TGTGGGAGTA ACGCGGTCAG TCAGAGCCGG GGCGGGCGGC GCGAGGNN // |
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Primer: BGH_rev2 Region: bGH p(A), nucleoplasmin NLS, Cas9 3' Sequence file: RDB17381_A9Fhc.seq
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>D06399A8_A9Fh_2_BGH_rev2_E02_05_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NGGGGGNNGG GCNNACNACA GNNGGCTGGC AACTAGAAGG 61 CACAGTCGAN GCTGATCAGC GAGCTCTAGG AATTCTTACT TTTTCTTTTT TGCCTGGCCG 121 GCCTTTTTCG TGGCCGCCGG CCTTTTGTCG CCTCCCAGCT GAGACAGGTC GATCCGTGTC 181 TCGTACAGGC CGGTGATGCT CTGGTGGATC AGGGTGGCGT CCAGCACCTC TTTGGTGCTG 241 GTGTACCTCT TCCGGTCGAT GGTGGTGTCA AAGTACTTGA AGGCGGCAGG GGCTCCCAGA 301 TTGGTCAGGG TAAACAGGTG GATGATATTC TCGGCCTGCT CTCTGATGGG CTTATCCCGG 361 TGCTTGTTGT AGGCGGACAG CACTTTGTCC AGATTAGCGT CGGCCAGGAT CACTCTCTTG 421 GAGAACTCGC TGATCTGCTC GATGATCTCG TCCAGGTAGT GCTTGTGCTG TTCCACAAAC 481 AGCTGTTTCT GCTCATTATC CTCGGGGGAG CCCTTCAGCT TCTCATAGTG GCTGGCCAGG 541 TACAGGAAGT TCACATATTT GGAGGGCAGG GCCAGTTCGT TTCCCTTCTG CAGTTCGCCG 601 GCAGAGGCCA GCATTCTCTT CCGGCCGTTT TCCAGCTCGA ACAGGGAGTA CTTAGGCAGC 661 TTGATGATCA GGTCCTTTTT CACTTCTTTG TAGCCCTTGG CTTCCAGAAA GTCGATGGGA 721 NTCTTCTCGA AGCTGCTTCT TTCCATGATG GTGATCCCCA GCAGCTCTTT CACACTCTTC 781 AGTTTCTTGG ACTTGCCCTT TTCCACTTTG GCCACCACCA GCACAGAATA GGCCACGGNN 841 GGGCTGTCGA ANCCGCCGTA CTTCTNANGG TCCCANNCCN TCTTTCTGGC GATCAGCTTA 901 TCGCNNTNCC NNTNGGGNAG GANANANTCN TNGCTGNNNN NNNNNN // |
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Sequencing and PCR primers for primer information.
Original, user report and related articles
| original | Unoki, M., CDCA7 and HELLS mutations undermine nonhomologous end joining in centromeric instability syndrome. J. Clin. Invest. 129 (1): 78-92 (2019). PMID 30307408. |
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2021.03.11
GNP_filter3_RDBDEP_html_210308.pl