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pEF-T7-GST-RBD32/38

Expression vector of ANKR1domain of mouse Ankrd27.

Catalog number RDB17371
Resource name pEF-T7-GST-RBD32/38
Clone info. Expression vector of ANKR1domain of mouse ankyrin repeat domain 27 (Ankrd27) tagged with T7-GST at N-terminus, EF1 promoter.
Vector backbone pEF-T7 (plasmid)
Selectable markers Amp^r.
Gene/insert name mouse Ankrd27 cDNA
Reference of nucleotide sequence AB455262 (DDBJ accession number)
Depositor Fukuda, Mitsunori |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Marubashi, S. et al. J. Biol. Chem. 291, 1427-1440, 2016); (Tamura, K. et al. Mol. Biol. Cell 20, 2900-2908, 2009)
Additional terms and conditions for distribution Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the pEF-BOS and pEF-BOS-EX vectors (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
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RDB17371 pEF-T7-GST-RBD32/38 Under QC test. Please contact us. DNA solution

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References and tips

Electronic file

Original reference

original Marubashi, S., RUTBC1 Functions as a GTPase-activating Protein for Rab32/38 and Regulates Melanogenic Enzyme Trafficking in Melanocytes. J. Biol. Chem. 291 (3): 1427-1440 (2016). PMID 26620560.

Further references such as user reports and related articles (go to bottom)

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Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

Original, user report and related articles

original Marubashi, S., RUTBC1 Functions as a GTPase-activating Protein for Rab32/38 and Regulates Melanogenic Enzyme Trafficking in Melanocytes. J. Biol. Chem. 291 (3): 1427-1440 (2016). PMID 26620560.
reference Tamura, K., Varp is a novel Rab32/38-binding protein that regulates Tyrp1 trafficking in melanocytes. Mol. Biol. Cell 20 (12): 2900-2908 (2009). PMID 19403694.

2019.07.17

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