Expression vector of ANKR1domain of mouse Ankrd27.
Clone info. | Expression vector of ANKR1domain of mouse ankyrin repeat domain 27 (Ankrd27) tagged with T7-GST at N-terminus, EF1 promoter. |
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Vector backbone | pEF-T7 (plasmid) |
Selectable markers | Amp^r. |
Gene/insert name | mouse Ankrd27 cDNA |
Reference of insert sequence | AB455262 (DDBJ accession number) |
Depositor|Developer | Fukuda, Mitsunori | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Marubashi, S. et al. J. Biol. Chem. 291, 1427-1440, 2016); (Tamura, K. et al. Mol. Biol. Cell 20, 2900-2908, 2009) Additional terms and conditions: Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the pEF-BOS and pEF-BOS-EX vectors (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested. |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Marubashi, S. et al. J. Biol. Chem. 291, 1427-1440, 2016); (Tamura, K. et al. Mol. Biol. Cell 20, 2900-2908, 2009)。 MTAに書く付加的使用条件: pEF-BOS and pEF-BOS-EXベクタについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクタを商業的に利用する場合、利用者は長田重一博士に連絡するものとする。利用者は、研究成果の公表にあたってpEF-BOSおよびpEF-BOS-EXベクタの文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990)を引用し、長田重一教授への謝辞の表明を必要とする。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB17371 | pEF-T7-GST-RBD32/38 | DNA solution |
JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge |
Materials & Methods section:
The pEF-T7-GST-RBD32/38 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17371). |
Reference section:
Marubashi, S., Shimada, H., Fukuda, M., Ohbayashi, N., RUTBC1 Functions as a GTPase-activating Protein for Rab32/38 and Regulates Melanogenic Enzyme Trafficking in Melanocytes. J. Biol. Chem. 291 (3): 1427-1440 (2016). PMID 26620560. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB17371_A9Fap1-1.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: EF1a-C_F (Pr0168) Region: EF-1 alpha pro,T7 tag,GST 5' Sequence file: RDB17371_A9Faa.seq ![]() |
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>D06377B6_A9Fa_1_EF1a-C_F_A01_01_ABI08.ab1 1 NNNNNNNNNN NNNNNNNTTT NANTTTGGNN CTTGGTTCNT TCTCAAGCCT CAGACAGTGG 61 TTCAAAGTTT TTTTCTTCCA TTTCAGGTGT CGTGAGGAAT TCTCTAGAGC GGCCGCGGGA 121 ATTCGATTCC ACCATGGCTA GCATGACTGG TGGACAGCAA ATGGGTCGCG GATCTATGTC 181 CCCTATACTA GGTTATTGGA AAATTAAGGG CCTTGTGCAA CCCACTCGAC TTCTTTTGGA 241 ATATCTTGAA GAAAAATATG AAGAGCATTT GTATGAGCGC GATGAAGGTG ATAAATGGCG 301 AAACAAAAAG TTTGAATTGG GTTTGGAGTT TCCCAATCTT CCTTATTATA TTGATGGTGA 361 TGTTAAATTA ACACAGTCTA TGGCCATCAT ACGTTATATA GCTGACAAGC ACAACATGTT 421 GGGTGGTTGT CCAAAAGAGC GTGCAGAGAT TTCAATGCTT GAAGGAGCGG TTTTGGATAT 481 TAGATACGGT GTTTCGAGAA TTGCATATAG TAAAGACTTT GAAACTCTCA AAGTTGATTT 541 TCTTAGCAAG CTACCTGAAA TGCTGAAAAT GTTCGAAGAT CGTTTATGTC ATAAAACATA 601 TTTAAATGGT GATCATGTAA CCCATCCTGA CTTCATGTTG TATGACGCTC TTGATGTTGT 661 TTTATACATG GACCCAATGT GCCTGGATGC GTTCCCAAAA TTAGTTTGTT TTAAAAAACG 721 TATTGAAGCT ATCCCACAAA TTGATAAGTA CTTGAAATCC AGCAAGTATA TAGCATGGCC 781 TTTGCANGGC TGGCAAGCCA CGTTTGGTGG TGGCGANCAT CCTCCAAAAT CGGATCTGGT 841 TCCGCGTGGA TCCGACCCGT CAGTTGTCNC TCCTTTCTCC AGANATGANA GANGTCAAAC 901 ACCCCTCCAT GTGGCTGCCC TCTGNGGNCA GGCATCCCTC ATTGACTTCC TGGTCTCCNA 961 ANGNGNNNNA NNNNNTGNNN NANANTATCA NGGGNNCACC CCCNTNANNT NNGGNNNGNN 1021 ANAANNNNTN NNNANNTGNG ANNCNNCTGN NNNNNNNNN // |
Primer: hGCSF_R (Pr0031) Region: hG-CSF3' UTR+pA,insert 3' Sequence file: RDB17371_A9Fab.seq ![]() |
>D06377B6_A9Fa_1_hGCSF_R_B01_02_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNAG NNNNNNNGGT GGGGNNCCTC ACTCTAGAGC GGCCGCGAAT 61 TCACTAGTGA TTTCAAATCC TGGCCAGTTT CTGAGCTGGA GCACACTTGG GACACTGGCA 121 TAAGGGGTGA CAGAATTCGA GATCCACTGT GCTGATGGCG TCTTCCACAT CGTCTAGGTC 181 ATCCTCTGTC CACTCCAAAA GGTATCGTAC CATTTCTAGA TCGCCATCAG CAACTGCCCT 241 TAGAAGCTTT TCTACCTCCC TGTAGTCCTT TTTGACCTCT TCTTGCCTGA AGCTCACCGA 301 TATGGAAGAA AAGCTGGAGG TGGAGGAGCC CTGGCTGATG GAATCCACAG AGCGTGTAGG 361 GGACTGTGCA GGGACCTCAG AAGGCCTTGG CCTCCTGTCA GAGGATAGGT GATGAGCTTC 421 CATGATAGAC AGAATCTTCG AGTTCAGAGC ACACTTGAGT GGTGTTTCCT TCAGTCTGTT 481 CTGGACTGCT GTGGGGGCTC CATTCTGCAG CAGTGTCTCT ATGATGCCCT CGTAACCCCA 541 GCGTGCAGCA ATGTGCAGGG CTGTGTCTCC TTTCTCATTA CCAATATCCA ATCTGCAGGC 601 CTGGACATCA TAGTAGACCA GGGCCTTCAC GCAATCCTCC TGCCCGTAGG TGCAGGCCAG 661 GTGCAGCGGG GTGTTGCCGT TGTTGTCCTG AACCTCAGTG CTGGCCTTGT AGTGCAGCAG 721 CAGCAGCGTC ACACTTTGGA AGCCTTTCTG ACATGCCAGA TGTAAGGGGG TGGANCCATG 781 ATAGTCTGTG GCATTCACTA CGGCACCTTT GGAGACCANG AAGTCNNTGA GGGATGCCTG 841 ACCNCAGAGG GCAGCCACAT GGNNGGGTGT TTGACCTCTG TCATCTCTGG ANAAANNANT 901 GACNACTGAN GGNTCGGANN NNCGCNNANN NAGATNCNAT TTTGNANGAN GGNCNNCNCC 961 NCCNAACNTG GNTTGCNNGC CCTGNNAANG NCATGCTATA TACTTGCNNG NNTNNNAGNN 1021 NTNNNNNNAN NTNNNGGGNN ANNNTNANNN NNNTTTTTTN AAANNNAANC TNNNTTTNNG 1081 NNNNNNNNNN // |
Primer: pGEX-F2 (Pr0403) Region: GST,thrombin site,insert 5' Sequence file: RDB17371_A9Fac.seq ![]() |
>D06377B6_A9Fa_1_pGEX-F2_B01_02_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNTA TTGNAGCTAT CCCACAAATT GATAAGTACT 61 TGAAATCCAG CAAGTATATA GCATGGCCTT TGCAGGGCTG GCAAGCCACG TTTGGTGGTG 121 GCGACCATCC TCCAAAATCG GATCTGGTTC CGCGTGGATC CGACCCGTCA GTTGTCACTC 181 CTTTCTCCAG AGATGACAGA GGTCAAACAC CCCTCCATGT GGCTGCCCTC TGTGGTCAGG 241 CATCCCTCAT TGACTTCCTG GTCTCCAAAG GTGCCGTAGT GAATGCCACA GACTATCATG 301 GGTCCACCCC CTTACATCTG GCATGTCAGA AAGGCTTCCA AAGTGTGACG CTGCTGCTGC 361 TGCACTACAA GGCCAGCACT GAGGTTCAGG ACAACAACGG CAACACCCCG CTGCACCTGG 421 CCTGCACCTA CGGGCAGGAG GATTGCGTGA AGGCCCTGGT CTACTATGAT GTCCAGGCCT 481 GCAGATTGGA TATTGGTAAT GAGAAAGGAG ACACAGCCCT GCACATTGCT GCACGCTGGG 541 GTTACGAGGG CATCATAGAG ACACTGCTGC AGAATGGAGC CCCCACAGCA GTCCAGAACA 601 GACTGAAGGA AACACCACTC AAGTGTGCTC TGAACTCGAA GATTCTGTCT ATCATGGAAG 661 CTCATCACCT ATCCTCTGAC AGGAGGCCAA GGCCTTCTGA GGTCCCTGCA CAGTCCCCTA 721 CACGCTCTGT GGATTCCATC AGCCAGGGCT CCTCCACCTC CAGCTTTTCT TCCATATCGG 781 TGAGCTTCAG GCAAGAAGAG GTCNAAAAGG ACTACAGGGN NGTAGAAAAG CTTCTAANGG 841 CAGTTGCTGA TGGCGATCTA NAANTGGTAC GATANCTTTT GNAGTGGANA GANGATGACC 901 TAGANNATGT GGAANANGCC NTCNGCACAG NGGATCTCGA ATTCTGNCNN CCCTNATGCC 961 NNNNTCCCAN GTGNGCTCCA GCTCNNANNN TGGNNAGNAN TTGAANTCAC TNNNGNANNN 1021 NCNNNCNGCN CTNNNNNGAG NNNCNNNNNT NGGNNNNNNN NNN // |
Primer: EF1a-M_R3 (Pr1255) Region: EF-1 alpha,SV40 pro_ori Sequence file: RDB17371_A9Fad.seq ![]() |
>D06377B6_A9Fa_1_EF1a-M_R3_H03_24_ABI24.ab1 1 NNNNNNNNNG NNNTCNNNNN NTTAAATGGC TAGAGACTTA TCGAAAGCAG CGAGACAGGC 61 GCGAAGGTGC CACCAGATTC GCACGCGGCG GCCCCAGCGC CCAGGCCAGG CCTCAACTCA 121 AGCACGAGGC GAAGGGGCTC CTTAAGCGCA AGGCCTCGAA CTCTCCCACC CACTTCCAAC 181 CCGAAGCTCG GGATCAAGAA TCACGTACTG CAGCCAGGTG GAAGTAATTC AAGGCACGCA 241 AGGGCCATAA CCCGTAAAGA GGCCAGGCCC GCGGGAACCA CACACGGCAC TTACCTGTGT 301 TCTGGCGGCA AACCCGTTGC GAAAAAGAAC GTTCACGGCG ACTACTGCAC TTATATACGG 361 TTCTCCCCCA CCCTCGGGAA AAAGGCGGAG CCAGTACACG ACATCACTTT CCCAGTTTAC 421 CCCGCGCCAC CTTCTCTAGG CACCGGTTCA ATTGCCGACC CCTCCCCCCA ACTTCTCGGG 481 GACTGTGGGC GATGTGCGCT CTGCCCACTG ACGGGCACCG GAGCCAATTC CCACTCCTTT 541 CAAGACCTAG AAGGTCCATT AGCTGCAAAG ATTCCTCTCT GTTTAAAACT TTATCCATCT 601 TTGCAAAGCT TTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT 661 AGCTCAGAGG CCGANGCGGC CTCGGCCTCT GCATAAATAA AAAAAANTAG TCAGCCATGG 721 GGCGGANAAT GGGCGGAACT GGGCGGANTT AGGGGCGGGA TGGGCGGANT TAGGGGCGGN 781 NNTATGGTTG CTGACTAATT GANANGCATG CTTTGCNTAC TTCTGNCTGC TGGGGANGGA 841 ANTAAGCTTG GCGTAANCAT GGNCATANNT GNTTNCTGTG TGAAATTGNN ATCCNNNNNN 901 NNNTNCNNNN NNNNTACNAN NNNGNNNNNT NAAGNGNNAN NNNNNGGNNN NNNNNNN // |
Primer: EF1a-M_F3 (Pr1242) Region: EF-1 alpha Sequence file: RDB17371_A9Fae.seq ![]() |
>D06377B6_A9Fa_1_EF1a-M_F3_E01_05_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNGCT TGANTTGANG CCTGGCCTGG GCGCTGGGGC 61 CGCCGCGTGC GAATCTGGTG GCACCTTCGC GCCTGTCTCG CTGCTTTCGA TAAGTCTCTA 121 GCCATTTAAA ATTTTTGATG ACCTGCTGCG ACGCTTTTTT TCTGGCAAGA TAGTCTTGTA 181 AATGCGGGCC AAGATCTGCA CACTGGTATT TCGGTTTTTG GGGCCGCGGG CGGCGACGGG 241 GCCCGTGCGT CCCAGCGCAC ATGTTCGGCG AGGCGGGGCC TGCGAGCGCG GCCACCGAGA 301 ATCGGACGGG GGTAGTCTCA AGCTGGCCGG CCTGCTCTGG TGCCTGGCCT CGCGCCGCCG 361 TGTATCGCCC CGCCCTGGGC GGCAAGGCTG GCCCGGTCGG CACCAGTTGC GTGAGCGGAA 421 AGATGGCCGC TTCCCGGCCC TGCTGCAGGG AGCTCAAAAT GGAGGACGCG GCGCTCGGGA 481 GAGCGGGCGG GTGAGTCACC CACACAAAGG AAAAGGGCCT TTCCGTCCTC AGCCGTCGCT 541 TCATGTGACT CCACGGAGTA CCGGGCGCCG TCCAGGCACC TCGATTAGTT CTCGAGCTTT 601 TGGAGTACGT CGTCTTTAGG TTGGGGGGAG GGGTTTTATG CGATGGAGTT TCCCCACACT 661 GAGTGGGTGG AGACTGNAAG TTAGGCCAGC TTGGCACTTG ATGTAATTCT CCTTGGAATT 721 TGCCCTTTTT GAGTTTGGAT CTTGGTTCAT TCTCNNNCCT CNNACAGTGG TTCAAAGTTT 781 TTTTCTTNNN NTTCNNGTGT CGTGAGGAAT TCTCTANAGC NGNNCGCNGG ANTTCNATTN 841 CNCNATGGCT ANCATGACTG GTGGANNGCA AATGGGTCGC GNATNTATGT CCCCTATNNT 901 NNNTTATTNG NAAAATNANN NNNNNNGTGN NNNCCNCNCN NNTTCTTTNN NNATATNNNN 961 GNANNAAANT ANNNANANNA TTTNNTATNA NCGCNANNNA NNNNGANNAN TGNNNNANNN 1021 AAAANNNNNN AANNNNNNNN NN // |
Primer: GST-N_rev (Pr0448) Region: GST 5',T7 tag,EF-1 alpha pro Sequence file: RDB17371_A9Faf.seq ![]() |
>D06377B6_A9Fa_1_GST-N_rev_C01_03_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNTCG AGTGGGTTGC ACAAGGCCCT TAATTTTCNN 61 ATAACCTAGT ATAGGGGACA TAGATCCGCG ACCCATTTGC TGTCCACCAG TCATGCTAGC 121 CATGGTGGAA TCGAATTCCC GCGGCCGCTC TAGAGAATTC CTCACGACAC CTGAAATGGA 181 AGAAAAAAAC TTTGAACCAC TGTCTGAGGC TTGAGAATGA ACCAAGATCC AAACTCAAAA 241 AGGGCAAATT CCAAGGAGAA TTACATCAAG TGCCAAGCTG GCCTAACTTC AGTCTCCACC 301 CACTCAGTGT GGGGAAACTC CATCGCATAA AACCCCTCCC CCCAACCTAA AGACGACGTA 361 CTCCAAAAGC TCGAGAACTA ATCGAGGTGC CTGGACGGCG CCCGGTACTC CGTGGAGTCA 421 CATGAAGCGA CGGCTGAGGA CGGAAAGGCC CTTTTCCTTT GTGTGGGTGA CTCACCCGCC 481 CGCTCTCCCG AGCGCCGCGT CCTCCATTTT GAGCTCCCTG CAGCAGGGCC GGGAAGCGGC 541 CATCTTTCCG CTCACGCAAC TGGTGCCGAC CGGGCCAGCC TTGCCGCCCA GGGCGGGGCG 601 ATACACGGCG GCGCGAGGCC AGGCACCAGA GCAGGCCGGC CAGCTTGAGA CTACCCCCGT 661 CCGATTCTCG GTGGCCGCGC TCGCAGGCCC CGCCTCGCCG AACATGTGCG CTGGGACGCA 721 CGGGCCCCGT CGCCGCCCGC GGCCCCNAAA ACCGAAATAC CAGTGTGCAG ATCTTGGCCC 781 GCATTTACNA GANTATCTTG CCAGAAAAAA AGCGTCGCAG CANGTCATCN AAAATTTTAA 841 ATGGNTAGAG NNTTATCGAA AGCAGCGAGA CAGGCGCGAA GGTGCCACCA GATTCNCACG 901 NNGNCGGNCC CAGCGCCCAN GNCNNGNNTC NNCTCNNGCA CGAGGCGAAN GGGNNCCTTA 961 ANCNCNNNNN NTCNAACTCT NCCACCCNNT TNCNNCCNNA ANCTCNGGNN NNNANNNNNN 1021 NNNN // |
Primer: RDB17371_F1 (Pr1293) Region: insert 3',hG-CSF3' UTR+pA Sequence file: RDB17371_A9Fag.seq ![]() |
>D06377B6_A9Fa_1_RDB17371_F1_A01_01_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NCTAGNNGAN GTGGAAGANG CCNTCAGCAC AGTGGATCTC 61 GAATTCTGTC ACCCCTTATG CCAGTGTCCC AAGTGTGCTC CAGCTCAGAA ACTGGCCAGG 121 ATTTGAAATC ACTAGTGAAT TCGCGGCCGC TCTAGAGTGA GGGTCCCCAC CTGGGACCCT 181 TGAGAGTATC AGGTCTCCCA CGTGGGAGAC AAGAAATCCC TGTTTAATAT TTAAACAGCA 241 GTGTTCCCCA TCTGGGTCCT TGCACCCCTC ACTCTGGCCT CAGCCGACTG CACAGCGGCC 301 CCTGCATCCC CTTGGCTGTG AGGCCCCTGG ACAAGCAGAG GTGGCCAGAG CTGGGAGGCA 361 TGGCCCTGGG GTCCCACGAA TTTGCTGGGG AATCTCGTTT TTCTTCTTAA GACTTTTGGG 421 ACATGGTTTG ACTCCCGAAC ATCACCGACG TGTCTCCTGT TTTTCTGGGT GGCCTCGGGA 481 CACCTGCCCT GCCCCCACGA GGGTCAGGAC TGTGACTCTT TTTAGGGCCA GGCAGGTGCC 541 TGGACATTTG CCTTGCTGGA CGGGGACTGG GGATGTGGGA GGGAGCAGAC AGGAGGAATC 601 ATGTCAGGCC TGTGTGTGAA AGGAAGCTCC ACTGTCACCC TCCACCTCTT CACCCCCCAC 661 TCACCAGTGT CCCCTCCACT GTCACATTGT AACTGAACTT CAGGATAATA AAGTGTTTGC 721 CTCNNAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAN NAANNNNNNN 781 AAAAAAAAAA NNNANAAAAA ANNNNNNNNN N // |
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Original, user report and related articles
original | Marubashi, S., RUTBC1 Functions as a GTPase-activating Protein for Rab32/38 and Regulates Melanogenic Enzyme Trafficking in Melanocytes. J. Biol. Chem. 291 (3): 1427-1440 (2016). PMID 26620560. [link to RRC of NBRP] |
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reference | Tamura, K., Varp is a novel Rab32/38-binding protein that regulates Tyrp1 trafficking in melanocytes. Mol. Biol. Cell 20 (12): 2900-2908 (2009). PMID 19403694. [link to RRC of NBRP] |
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