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pEF-T7-GST-RBD32/38 (#RDB17371)

Expression vector of ANKR1domain of mouse Ankrd27.

Clone info. Expression vector of ANKR1domain of mouse ankyrin repeat domain 27 (Ankrd27) tagged with T7-GST at N-terminus, EF1 promoter.
Vector backbone pEF-T7 (plasmid)
Selectable markers Amp^r.
Gene/insert name mouse Ankrd27 cDNA
Reference of insert sequence AB455262 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Marubashi, S. et al. J. Biol. Chem. 291, 1427-1440, 2016); (Tamura, K. et al. Mol. Biol. Cell 20, 2900-2908, 2009)
Additional terms and conditions:
Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the pEF-BOS and pEF-BOS-EX vectors (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Marubashi, S. et al. J. Biol. Chem. 291, 1427-1440, 2016); (Tamura, K. et al. Mol. Biol. Cell 20, 2900-2908, 2009)。
MTAに書く付加的使用条件:
pEF-BOS and pEF-BOS-EXベクタについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクタを商業的に利用する場合、利用者は長田重一博士に連絡するものとする。利用者は、研究成果の公表にあたってpEF-BOSおよびpEF-BOS-EXベクタの文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990)を引用し、長田重一教授への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17371 pEF-T7-GST-RBD32/38 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEF-T7-GST-RBD32/38 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17371).

Reference section:

Marubashi, S., Shimada, H., Fukuda, M., Ohbayashi, N., RUTBC1 Functions as a GTPase-activating Protein for Rab32/38 and Regulates Melanogenic Enzyme Trafficking in Melanocytes. J. Biol. Chem. 291 (3): 1427-1440 (2016). PMID 26620560. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17371_A9Fap1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168)
Region: EF-1 alpha pro,T7 tag,GST 5'
Sequence file: RDB17371_A9Faa.seq check
>D06377B6_A9Fa_1_EF1a-C_F_A01_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNTTT NANTTTGGNN CTTGGTTCNT TCTCAAGCCT CAGACAGTGG
   61 TTCAAAGTTT TTTTCTTCCA TTTCAGGTGT CGTGAGGAAT TCTCTAGAGC GGCCGCGGGA
  121 ATTCGATTCC ACCATGGCTA GCATGACTGG TGGACAGCAA ATGGGTCGCG GATCTATGTC
  181 CCCTATACTA GGTTATTGGA AAATTAAGGG CCTTGTGCAA CCCACTCGAC TTCTTTTGGA
  241 ATATCTTGAA GAAAAATATG AAGAGCATTT GTATGAGCGC GATGAAGGTG ATAAATGGCG
  301 AAACAAAAAG TTTGAATTGG GTTTGGAGTT TCCCAATCTT CCTTATTATA TTGATGGTGA
  361 TGTTAAATTA ACACAGTCTA TGGCCATCAT ACGTTATATA GCTGACAAGC ACAACATGTT
  421 GGGTGGTTGT CCAAAAGAGC GTGCAGAGAT TTCAATGCTT GAAGGAGCGG TTTTGGATAT
  481 TAGATACGGT GTTTCGAGAA TTGCATATAG TAAAGACTTT GAAACTCTCA AAGTTGATTT
  541 TCTTAGCAAG CTACCTGAAA TGCTGAAAAT GTTCGAAGAT CGTTTATGTC ATAAAACATA
  601 TTTAAATGGT GATCATGTAA CCCATCCTGA CTTCATGTTG TATGACGCTC TTGATGTTGT
  661 TTTATACATG GACCCAATGT GCCTGGATGC GTTCCCAAAA TTAGTTTGTT TTAAAAAACG
  721 TATTGAAGCT ATCCCACAAA TTGATAAGTA CTTGAAATCC AGCAAGTATA TAGCATGGCC
  781 TTTGCANGGC TGGCAAGCCA CGTTTGGTGG TGGCGANCAT CCTCCAAAAT CGGATCTGGT
  841 TCCGCGTGGA TCCGACCCGT CAGTTGTCNC TCCTTTCTCC AGANATGANA GANGTCAAAC
  901 ACCCCTCCAT GTGGCTGCCC TCTGNGGNCA GGCATCCCTC ATTGACTTCC TGGTCTCCNA
  961 ANGNGNNNNA NNNNNTGNNN NANANTATCA NGGGNNCACC CCCNTNANNT NNGGNNNGNN
1021 ANAANNNNTN NNNANNTGNG ANNCNNCTGN NNNNNNNNN
//
Primer: hGCSF_R (Pr0031)
Region: hG-CSF3' UTR+pA,insert 3'
Sequence file: RDB17371_A9Fab.seq check
>D06377B6_A9Fa_1_hGCSF_R_B01_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNAG NNNNNNNGGT GGGGNNCCTC ACTCTAGAGC GGCCGCGAAT
   61 TCACTAGTGA TTTCAAATCC TGGCCAGTTT CTGAGCTGGA GCACACTTGG GACACTGGCA
  121 TAAGGGGTGA CAGAATTCGA GATCCACTGT GCTGATGGCG TCTTCCACAT CGTCTAGGTC
  181 ATCCTCTGTC CACTCCAAAA GGTATCGTAC CATTTCTAGA TCGCCATCAG CAACTGCCCT
  241 TAGAAGCTTT TCTACCTCCC TGTAGTCCTT TTTGACCTCT TCTTGCCTGA AGCTCACCGA
  301 TATGGAAGAA AAGCTGGAGG TGGAGGAGCC CTGGCTGATG GAATCCACAG AGCGTGTAGG
  361 GGACTGTGCA GGGACCTCAG AAGGCCTTGG CCTCCTGTCA GAGGATAGGT GATGAGCTTC
  421 CATGATAGAC AGAATCTTCG AGTTCAGAGC ACACTTGAGT GGTGTTTCCT TCAGTCTGTT
  481 CTGGACTGCT GTGGGGGCTC CATTCTGCAG CAGTGTCTCT ATGATGCCCT CGTAACCCCA
  541 GCGTGCAGCA ATGTGCAGGG CTGTGTCTCC TTTCTCATTA CCAATATCCA ATCTGCAGGC
  601 CTGGACATCA TAGTAGACCA GGGCCTTCAC GCAATCCTCC TGCCCGTAGG TGCAGGCCAG
  661 GTGCAGCGGG GTGTTGCCGT TGTTGTCCTG AACCTCAGTG CTGGCCTTGT AGTGCAGCAG
  721 CAGCAGCGTC ACACTTTGGA AGCCTTTCTG ACATGCCAGA TGTAAGGGGG TGGANCCATG
  781 ATAGTCTGTG GCATTCACTA CGGCACCTTT GGAGACCANG AAGTCNNTGA GGGATGCCTG
  841 ACCNCAGAGG GCAGCCACAT GGNNGGGTGT TTGACCTCTG TCATCTCTGG ANAAANNANT
  901 GACNACTGAN GGNTCGGANN NNCGCNNANN NAGATNCNAT TTTGNANGAN GGNCNNCNCC
  961 NCCNAACNTG GNTTGCNNGC CCTGNNAANG NCATGCTATA TACTTGCNNG NNTNNNAGNN
 1021 NTNNNNNNAN NTNNNGGGNN ANNNTNANNN NNNTTTTTTN AAANNNAANC TNNNTTTNNG
1081 NNNNNNNNNN
//
Primer: pGEX-F2 (Pr0403)
Region: GST,thrombin site,insert 5'
Sequence file: RDB17371_A9Fac.seq check
>D06377B6_A9Fa_1_pGEX-F2_B01_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNTA TTGNAGCTAT CCCACAAATT GATAAGTACT
   61 TGAAATCCAG CAAGTATATA GCATGGCCTT TGCAGGGCTG GCAAGCCACG TTTGGTGGTG
  121 GCGACCATCC TCCAAAATCG GATCTGGTTC CGCGTGGATC CGACCCGTCA GTTGTCACTC
  181 CTTTCTCCAG AGATGACAGA GGTCAAACAC CCCTCCATGT GGCTGCCCTC TGTGGTCAGG
  241 CATCCCTCAT TGACTTCCTG GTCTCCAAAG GTGCCGTAGT GAATGCCACA GACTATCATG
  301 GGTCCACCCC CTTACATCTG GCATGTCAGA AAGGCTTCCA AAGTGTGACG CTGCTGCTGC
  361 TGCACTACAA GGCCAGCACT GAGGTTCAGG ACAACAACGG CAACACCCCG CTGCACCTGG
  421 CCTGCACCTA CGGGCAGGAG GATTGCGTGA AGGCCCTGGT CTACTATGAT GTCCAGGCCT
  481 GCAGATTGGA TATTGGTAAT GAGAAAGGAG ACACAGCCCT GCACATTGCT GCACGCTGGG
  541 GTTACGAGGG CATCATAGAG ACACTGCTGC AGAATGGAGC CCCCACAGCA GTCCAGAACA
  601 GACTGAAGGA AACACCACTC AAGTGTGCTC TGAACTCGAA GATTCTGTCT ATCATGGAAG
  661 CTCATCACCT ATCCTCTGAC AGGAGGCCAA GGCCTTCTGA GGTCCCTGCA CAGTCCCCTA
  721 CACGCTCTGT GGATTCCATC AGCCAGGGCT CCTCCACCTC CAGCTTTTCT TCCATATCGG
  781 TGAGCTTCAG GCAAGAAGAG GTCNAAAAGG ACTACAGGGN NGTAGAAAAG CTTCTAANGG
  841 CAGTTGCTGA TGGCGATCTA NAANTGGTAC GATANCTTTT GNAGTGGANA GANGATGACC
  901 TAGANNATGT GGAANANGCC NTCNGCACAG NGGATCTCGA ATTCTGNCNN CCCTNATGCC
  961 NNNNTCCCAN GTGNGCTCCA GCTCNNANNN TGGNNAGNAN TTGAANTCAC TNNNGNANNN
1021 NCNNNCNGCN CTNNNNNGAG NNNCNNNNNT NGGNNNNNNN NNN
//
Primer: EF1a-M_R3 (Pr1255)
Region: EF-1 alpha,SV40 pro_ori
Sequence file: RDB17371_A9Fad.seq check
>D06377B6_A9Fa_1_EF1a-M_R3_H03_24_ABI24.ab1
    1 NNNNNNNNNG NNNTCNNNNN NTTAAATGGC TAGAGACTTA TCGAAAGCAG CGAGACAGGC
   61 GCGAAGGTGC CACCAGATTC GCACGCGGCG GCCCCAGCGC CCAGGCCAGG CCTCAACTCA
  121 AGCACGAGGC GAAGGGGCTC CTTAAGCGCA AGGCCTCGAA CTCTCCCACC CACTTCCAAC
  181 CCGAAGCTCG GGATCAAGAA TCACGTACTG CAGCCAGGTG GAAGTAATTC AAGGCACGCA
  241 AGGGCCATAA CCCGTAAAGA GGCCAGGCCC GCGGGAACCA CACACGGCAC TTACCTGTGT
  301 TCTGGCGGCA AACCCGTTGC GAAAAAGAAC GTTCACGGCG ACTACTGCAC TTATATACGG
  361 TTCTCCCCCA CCCTCGGGAA AAAGGCGGAG CCAGTACACG ACATCACTTT CCCAGTTTAC
  421 CCCGCGCCAC CTTCTCTAGG CACCGGTTCA ATTGCCGACC CCTCCCCCCA ACTTCTCGGG
  481 GACTGTGGGC GATGTGCGCT CTGCCCACTG ACGGGCACCG GAGCCAATTC CCACTCCTTT
  541 CAAGACCTAG AAGGTCCATT AGCTGCAAAG ATTCCTCTCT GTTTAAAACT TTATCCATCT
  601 TTGCAAAGCT TTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT
  661 AGCTCAGAGG CCGANGCGGC CTCGGCCTCT GCATAAATAA AAAAAANTAG TCAGCCATGG
  721 GGCGGANAAT GGGCGGAACT GGGCGGANTT AGGGGCGGGA TGGGCGGANT TAGGGGCGGN
  781 NNTATGGTTG CTGACTAATT GANANGCATG CTTTGCNTAC TTCTGNCTGC TGGGGANGGA
  841 ANTAAGCTTG GCGTAANCAT GGNCATANNT GNTTNCTGTG TGAAATTGNN ATCCNNNNNN
901 NNNTNCNNNN NNNNTACNAN NNNGNNNNNT NAAGNGNNAN NNNNNGGNNN NNNNNNN
//
Primer: EF1a-M_F3 (Pr1242)
Region: EF-1 alpha
Sequence file: RDB17371_A9Fae.seq check
>D06377B6_A9Fa_1_EF1a-M_F3_E01_05_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNGCT TGANTTGANG CCTGGCCTGG GCGCTGGGGC
   61 CGCCGCGTGC GAATCTGGTG GCACCTTCGC GCCTGTCTCG CTGCTTTCGA TAAGTCTCTA
  121 GCCATTTAAA ATTTTTGATG ACCTGCTGCG ACGCTTTTTT TCTGGCAAGA TAGTCTTGTA
  181 AATGCGGGCC AAGATCTGCA CACTGGTATT TCGGTTTTTG GGGCCGCGGG CGGCGACGGG
  241 GCCCGTGCGT CCCAGCGCAC ATGTTCGGCG AGGCGGGGCC TGCGAGCGCG GCCACCGAGA
  301 ATCGGACGGG GGTAGTCTCA AGCTGGCCGG CCTGCTCTGG TGCCTGGCCT CGCGCCGCCG
  361 TGTATCGCCC CGCCCTGGGC GGCAAGGCTG GCCCGGTCGG CACCAGTTGC GTGAGCGGAA
  421 AGATGGCCGC TTCCCGGCCC TGCTGCAGGG AGCTCAAAAT GGAGGACGCG GCGCTCGGGA
  481 GAGCGGGCGG GTGAGTCACC CACACAAAGG AAAAGGGCCT TTCCGTCCTC AGCCGTCGCT
  541 TCATGTGACT CCACGGAGTA CCGGGCGCCG TCCAGGCACC TCGATTAGTT CTCGAGCTTT
  601 TGGAGTACGT CGTCTTTAGG TTGGGGGGAG GGGTTTTATG CGATGGAGTT TCCCCACACT
  661 GAGTGGGTGG AGACTGNAAG TTAGGCCAGC TTGGCACTTG ATGTAATTCT CCTTGGAATT
  721 TGCCCTTTTT GAGTTTGGAT CTTGGTTCAT TCTCNNNCCT CNNACAGTGG TTCAAAGTTT
  781 TTTTCTTNNN NTTCNNGTGT CGTGAGGAAT TCTCTANAGC NGNNCGCNGG ANTTCNATTN
  841 CNCNATGGCT ANCATGACTG GTGGANNGCA AATGGGTCGC GNATNTATGT CCCCTATNNT
  901 NNNTTATTNG NAAAATNANN NNNNNNGTGN NNNCCNCNCN NNTTCTTTNN NNATATNNNN
  961 GNANNAAANT ANNNANANNA TTTNNTATNA NCGCNANNNA NNNNGANNAN TGNNNNANNN
1021 AAAANNNNNN AANNNNNNNN NN
//
Primer: GST-N_rev (Pr0448)
Region: GST 5',T7 tag,EF-1 alpha pro
Sequence file: RDB17371_A9Faf.seq check
>D06377B6_A9Fa_1_GST-N_rev_C01_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNTCG AGTGGGTTGC ACAAGGCCCT TAATTTTCNN
   61 ATAACCTAGT ATAGGGGACA TAGATCCGCG ACCCATTTGC TGTCCACCAG TCATGCTAGC
  121 CATGGTGGAA TCGAATTCCC GCGGCCGCTC TAGAGAATTC CTCACGACAC CTGAAATGGA
  181 AGAAAAAAAC TTTGAACCAC TGTCTGAGGC TTGAGAATGA ACCAAGATCC AAACTCAAAA
  241 AGGGCAAATT CCAAGGAGAA TTACATCAAG TGCCAAGCTG GCCTAACTTC AGTCTCCACC
  301 CACTCAGTGT GGGGAAACTC CATCGCATAA AACCCCTCCC CCCAACCTAA AGACGACGTA
  361 CTCCAAAAGC TCGAGAACTA ATCGAGGTGC CTGGACGGCG CCCGGTACTC CGTGGAGTCA
  421 CATGAAGCGA CGGCTGAGGA CGGAAAGGCC CTTTTCCTTT GTGTGGGTGA CTCACCCGCC
  481 CGCTCTCCCG AGCGCCGCGT CCTCCATTTT GAGCTCCCTG CAGCAGGGCC GGGAAGCGGC
  541 CATCTTTCCG CTCACGCAAC TGGTGCCGAC CGGGCCAGCC TTGCCGCCCA GGGCGGGGCG
  601 ATACACGGCG GCGCGAGGCC AGGCACCAGA GCAGGCCGGC CAGCTTGAGA CTACCCCCGT
  661 CCGATTCTCG GTGGCCGCGC TCGCAGGCCC CGCCTCGCCG AACATGTGCG CTGGGACGCA
  721 CGGGCCCCGT CGCCGCCCGC GGCCCCNAAA ACCGAAATAC CAGTGTGCAG ATCTTGGCCC
  781 GCATTTACNA GANTATCTTG CCAGAAAAAA AGCGTCGCAG CANGTCATCN AAAATTTTAA
  841 ATGGNTAGAG NNTTATCGAA AGCAGCGAGA CAGGCGCGAA GGTGCCACCA GATTCNCACG
  901 NNGNCGGNCC CAGCGCCCAN GNCNNGNNTC NNCTCNNGCA CGAGGCGAAN GGGNNCCTTA
  961 ANCNCNNNNN NTCNAACTCT NCCACCCNNT TNCNNCCNNA ANCTCNGGNN NNNANNNNNN
1021 NNNN
//
Primer: RDB17371_F1 (Pr1293)
Region: insert 3',hG-CSF3' UTR+pA
Sequence file: RDB17371_A9Fag.seq check
>D06377B6_A9Fa_1_RDB17371_F1_A01_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NCTAGNNGAN GTGGAAGANG CCNTCAGCAC AGTGGATCTC
   61 GAATTCTGTC ACCCCTTATG CCAGTGTCCC AAGTGTGCTC CAGCTCAGAA ACTGGCCAGG
  121 ATTTGAAATC ACTAGTGAAT TCGCGGCCGC TCTAGAGTGA GGGTCCCCAC CTGGGACCCT
  181 TGAGAGTATC AGGTCTCCCA CGTGGGAGAC AAGAAATCCC TGTTTAATAT TTAAACAGCA
  241 GTGTTCCCCA TCTGGGTCCT TGCACCCCTC ACTCTGGCCT CAGCCGACTG CACAGCGGCC
  301 CCTGCATCCC CTTGGCTGTG AGGCCCCTGG ACAAGCAGAG GTGGCCAGAG CTGGGAGGCA
  361 TGGCCCTGGG GTCCCACGAA TTTGCTGGGG AATCTCGTTT TTCTTCTTAA GACTTTTGGG
  421 ACATGGTTTG ACTCCCGAAC ATCACCGACG TGTCTCCTGT TTTTCTGGGT GGCCTCGGGA
  481 CACCTGCCCT GCCCCCACGA GGGTCAGGAC TGTGACTCTT TTTAGGGCCA GGCAGGTGCC
  541 TGGACATTTG CCTTGCTGGA CGGGGACTGG GGATGTGGGA GGGAGCAGAC AGGAGGAATC
  601 ATGTCAGGCC TGTGTGTGAA AGGAAGCTCC ACTGTCACCC TCCACCTCTT CACCCCCCAC
  661 TCACCAGTGT CCCCTCCACT GTCACATTGT AACTGAACTT CAGGATAATA AAGTGTTTGC
  721 CTCNNAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAN NAANNNNNNN
781 AAAAAAAAAA NNNANAAAAA ANNNNNNNNN N
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Marubashi, S., RUTBC1 Functions as a GTPase-activating Protein for Rab32/38 and Regulates Melanogenic Enzyme Trafficking in Melanocytes. J. Biol. Chem. 291 (3): 1427-1440 (2016). PMID 26620560. [link to RRC of NBRP]
reference Tamura, K., Varp is a novel Rab32/38-binding protein that regulates Tyrp1 trafficking in melanocytes. Mol. Biol. Cell 20 (12): 2900-2908 (2009). PMID 19403694. [link to RRC of NBRP]