Expression vector of mouse HPS4, Rab32/38-GEF-activity-deficient mutant (G55W/G59M).
Clone info. | Expression vector of mouse hermansky-pudlak syndrome 4 (HPS4) Rab32/38-GEF-activity-deficient mutant (G55W/G59M), tagged withT7 at N-terminus, EF1 promoter. GEF, gunanine nucleotide exchange factor. |
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Vector backbone | pEF-T7 (plasmid) |
Selectable markers | Amp^r. |
Gene/insert name | mouse Hps4 cDNA |
Depositor|Developer | Fukuda, Mitsunori | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Marubashi, S. et al. J. Biol. Chem. 291, 1427-1440, 2016); (Ohishi et al. J. Biol. Chem. 294, 6912-6922, 2019) Additional terms and conditions: Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the pEF-BOS and pEF-BOS-EX vectors (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested. |
必要書類 | 提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Marubashi, S. et al. J. Biol. Chem. 291, 1427-1440, 2016); (Ohishi et al. J. Biol. Chem. 294, 6912-6922, 2019)。 MTAに書く付加的使用条件: pEF-BOS and pEF-BOS-EXベクタについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクタを商業的に利用する場合、利用者は長田重一博士に連絡するものとする。利用者は、研究成果の公表にあたってpEF-BOSおよびpEF-BOS-EXベクタの文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990)を引用し、長田重一教授への謝辞の表明を必要とする。 |
Catalog # | Resource name | Availability | Shipping form | Fee (non-profit org.) |
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RDB17368 | pEF-T7-HPS4(G55W/G59M) | Under QC test. Please contact us. | DNA solution |
JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge |
Materials & Methods section:
The pEF-T7-HPS4(G55W/G59M) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17368). |
Reference section:
Ohishi, Y., Kinoshita, R., Marubashi, S., Ishida, M., Fukuda, M., The BLOC-3 subunit HPS4 is required for activation of Rab32/38 GTPases in melanogenesis, but its Rab9 activity is dispensable for melanogenesis. J. Biol. Chem. 294 (17): 6912-6922 (2019). PMID 30837268. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Original, user report and related articles
original | Ohishi, Y., The BLOC-3 subunit HPS4 is required for activation of Rab32/38 GTPases in melanogenesis, but its Rab9 activity is dispensable for melanogenesis. J. Biol. Chem. 294 (17): 6912-6922 (2019). PMID 30837268. [link to RRC of NBRP] |
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reference | Marubashi, S., RUTBC1 Functions as a GTPase-activating Protein for Rab32/38 and Regulates Melanogenic Enzyme Trafficking in Melanocytes. J. Biol. Chem. 291 (3): 1427-1440 (2016). PMID 26620560. [link to RRC of NBRP] |
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