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pEF-T7-HPS4(G55W/G59M)

Expression vector of mouse HPS4, Rab32/38-GEF-activity-deficient mutant (G55W/G59M).

Catalog number RDB17368
Resource name pEF-T7-HPS4(G55W/G59M)
Clone info. Expression vector of mouse hermansky-pudlak syndrome 4 (HPS4) Rab32/38-GEF-activity-deficient mutant (G55W/G59M), tagged withT7 at N-terminus, EF1 promoter. GEF, gunanine nucleotide exchange factor.
Vector backbone pEF-T7 (plasmid)
Selectable markers Amp^r.
Gene/insert name mouse Hps4 cDNA
Depositor Fukuda, Mitsunori |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Marubashi, S. et al. J. Biol. Chem. 291, 1427-1440, 2016); (Ohishi et al. J. Biol. Chem. 294, 6912-6922, 2019)
Additional terms and conditions for distribution Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the pEF-BOS and pEF-BOS-EX vectors (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
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Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB17368 pEF-T7-HPS4(G55W/G59M) Under QC test. Please contact us. DNA solution

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References and tips

Electronic file

Original reference

original Ohishi, Y., The BLOC-3 subunit HPS4 is required for activation of Rab32/38 GTPases in melanogenesis, but its Rab9 activity is dispensable for melanogenesis. J. Biol. Chem. 294 (17): 6912-6922 (2019). PMID 30837268.

Further references such as user reports and related articles (go to bottom)

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Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

Original, user report and related articles

original Ohishi, Y., The BLOC-3 subunit HPS4 is required for activation of Rab32/38 GTPases in melanogenesis, but its Rab9 activity is dispensable for melanogenesis. J. Biol. Chem. 294 (17): 6912-6922 (2019). PMID 30837268.
reference Marubashi, S., RUTBC1 Functions as a GTPase-activating Protein for Rab32/38 and Regulates Melanogenic Enzyme Trafficking in Melanocytes. J. Biol. Chem. 291 (3): 1427-1440 (2016). PMID 26620560.

2019.07.17

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