RDB17368 - pEF-T7-HPS4(G55W/G59M)

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pEF-T7-HPS4(G55W/G59M)

Expression vector of mouse HPS4, Rab32/38-GEF-activity-deficient mutant (G55W/G59M).

Catalog number	RDB17368
Resource name	pEF-T7-HPS4(G55W/G59M)
Clone info.	Expression vector of mouse hermansky-pudlak syndrome 4 (HPS4) Rab32/38-GEF-activity-deficient mutant (G55W/G59M), tagged withT7 at N-terminus, EF1 promoter. GEF, gunanine nucleotide exchange factor.
Vector backbone	pEF-T7 (plasmid)
Selectable markers	Amp^r.
Gene/insert name	mouse Hps4 cDNA
Depositor\|Developer	Fukuda, Mitsunori \|

Other clones in our bank

mouse Hps4

External Database
mouse Hps4

Reference sequence

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Marubashi, S. et al. J. Biol. Chem. 291, 1427-1440, 2016); (Ohishi et al. J. Biol. Chem. 294, 6912-6922, 2019)
Additional terms and conditions:
Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the pEF-BOS and pEF-BOS-EX vectors (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.

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提供案内 (日本国内) [open/close]

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Marubashi, S. et al. J. Biol. Chem. 291, 1427-1440, 2016); (Ohishi et al. J. Biol. Chem. 294, 6912-6922, 2019) Additional terms and conditions: Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the pEF-BOS and pEF-BOS-EX vectors (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
Ordering	Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Please visit Information of Request for Distribution.[link]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Marubashi, S. et al. J. Biol. Chem. 291, 1427-1440, 2016); (Ohishi et al. J. Biol. Chem. 294, 6912-6922, 2019)。付加的提供条件： pEF-BOS and pEF-BOS-EXベクタについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクタを商業的に利用する場合、利用者は長田重一博士に連絡するものとする。利用者は、研究成果の公表にあたってpEF-BOSおよびpEF-BOS-EXベクタの文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990)を引用し、長田重一教授への謝辞の表明を必要とする。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。

提供条件

利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Marubashi, S. et al. J. Biol. Chem. 291, 1427-1440, 2016); (Ohishi et al. J. Biol. Chem. 294, 6912-6922, 2019)。
付加的提供条件：
pEF-BOS and pEF-BOS-EXベクタについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクタを商業的に利用する場合、利用者は長田重一博士に連絡するものとする。利用者は、研究成果の公表にあたってpEF-BOSおよびpEF-BOS-EXベクタの文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990)を引用し、長田重一教授への謝辞の表明を必要とする。

提供依頼

提供依頼書 [Word

]
提供同意書 (MTA、非営利機関による非営利学術研究用)[Word

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。

Catalog #	Resource name	Availability	Shipping form	Fee (non-profit org.)
RDB17368	pEF-T7-HPS4(G55W/G59M)	Under QC test. Please contact us.	DNA solution

Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pEF-T7-HPS4(G55W/G59M) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17368).

Reference section:

Ohishi, Y., Kinoshita, R., Marubashi, S., Ishida, M., Fukuda, M., The BLOC-3 subunit HPS4 is required for activation of Rab32/38 GTPases in melanogenesis, but its Rab9 activity is dispensable for melanogenesis. J. Biol. Chem. 294 (17): 6912-6922 (2019). PMID 30837268. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

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QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original	Ohishi, Y., The BLOC-3 subunit HPS4 is required for activation of Rab32/38 GTPases in melanogenesis, but its Rab9 activity is dispensable for melanogenesis. J. Biol. Chem. 294 (17): 6912-6922 (2019). PMID 30837268. [link to RRC of NBRP]
reference	Marubashi, S., RUTBC1 Functions as a GTPase-activating Protein for Rab32/38 and Regulates Melanogenic Enzyme Trafficking in Melanocytes. J. Biol. Chem. 291 (3): 1427-1440 (2016). PMID 26620560. [link to RRC of NBRP]

2023.08.17

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