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pMRX-bsr-ss-EGFP-FM4-TMD

Retroviral expression vector enabling a synchronized trafficking assay.

Catalog number RDB17365
Resource name pMRX-bsr-ss-EGFP-FM4-TMD
Clone info. Retroviral expression vector enabling a synchronized trafficking assay, EGFP tagged with the signal sequence from mouse calreticulin (ss) at its N-terminus and FM4-TMD at its C terminus. FM4, 4 x mouse FK506 binding protein 1a (FKBP1A/FKBP12) mutant (Phe-to-Met substitution at amino acid position 37). TMD, transmembrane domain from human low density lipoprotein receptor (LDLR). See Fig.5A of Homma, Y. et al. J. Cell Biol. 218 (6): 2035-2050, 2019.
Vector backbone pMRX-IRES-bsr (plasmid)
Selectable markers Amp^r (E. coli), Blasticidin^r (mammalian cells).
Gene/insert name human LDLR cDNA mouse Calr cDNA mouse Fkbp1a cDNA
Depositor|Developer Fukuda, Mitsunori |
 
Other clones in our bank human LDLR (NCBI Gene 3949) | mouse Calr (NCBI Gene 12317) | mouse Fkbp1a (NCBI Gene 14225) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Hirano, S. et al. Autophagy 12, 312-326 (FM4), 2016); (Homma, Y. et al. J. Cell Biol., in press (doi: 10.1083/jcb.201810134), 2019)
Additional terms and conditions for distribution Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested.
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RDB17365 pMRX-bsr-ss-EGFP-FM4-TMD DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Homma, Y., Comprehensive knockout analysis of the Rab family GTPases in epithelial cells. J. Cell Biol. 218 (6): 2035-2050 (2019). PMID 31072826.
original Hirano, S., Differing susceptibility to autophagic degradation of two LC3-binding proteins: SQSTM1/p62 and TBC1D25/OATL1. Autophagy 12 (2): 312-326 (2016). PMID 26902585.

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17365_A9Lgp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: MMLV-pol_F
Region: pol region, SS, EGFP 5'
Sequence file: RDB17365_A9Lga.seq check
>D06377A9_A9Lg_1_MMLV-pol_F_A09_03_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNGGN ATCGCAGCTT GGNNACNCGC CGCCCACGTG AAGGCTGCCG
   61 ACCCCGGGGG TGGACCATCC TCTAGACTGC CGGATCTGCC ACCATGCTCC TTTCGGTGCC
  121 GCTCCTGCTT GGCCTCCTCG GCCTGGCCGC CGCAAGATCC ATGGTGAGCA AGGGCGAGGA
  181 GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA
  241 GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA CCCTGAAGTT
  301 CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCCTGACCTA
  361 CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC
  421 CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG ACGGCAACTA
  481 CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA
  541 GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTACAA
  601 CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC GGCATCAAGG TGAACTTCAA
  661 GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC AGCAGAACAC
  721 CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGANCA CCCAGTCCGC
  781 CCTGNNCAAA GANCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGANT TCGTGACCGC
  841 CGCCGGGNTC ACTCTCGGCA TGGACGANCT GTACAAGGGN TCCGGANNGC ANGTGGAGNN
  901 CATCTCTNCN GNANNNNGGC GCANNTTNCC NANGCGCGGN CANANCNGCN NNGNNNCANT
961 ACNNNGGNNN NNNNNNN
//
Primer: EGFP-C2
Region: EGFP 3', FM(1), FM(2), FM(3)
Sequence file: RDB17365_A9Lgb.seq check
>D06377A9_A9Lg_1_EGFP-C2_F09_18_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN GGTCCTGCTG GAGTTCGTGN NCGCCGCCGG
   61 GATCACTCTC GGCATGGNNN AGCTGTACAA GGGATCCGGA GTGCAGGTGG AGACCATCTC
  121 TCCTGGAGAC GGGCGCACCT TCCCAAAGCG CGGCCAGACC TGCGTGGTGC ACTACACGGG
  181 GATGCTTGAA GATGGAAAGA AAATGGATTC CTCTCGGGAC AGAAACAAGC CTTTTAAGTT
  241 TACACTAGGC AAGCAGGAGG TGATCCGAGG CTGGGAGGAA GGGGTAGCCC AGATGAGTGT
  301 GGGTCAGAGA GCCAAACTGA TAATCTCCTC AGACTATGCC TATGGAGCCA CCGGGCACCC
  361 AGGCATCATC CCACCACATG CCACTCTTGT TTTTGATGTG GAGCTTCTAA AACTGGAAGG
  421 AAGATCCGGA GTGCAGGTGG AGACCATCTC TCCTGGAGAC GGGCGCACCT TCCCAAAGCG
  481 CGGCCAGACC TGCGTGGTGC ACTACACGGG GATGCTTGAA GATGGAAAGA AAATGGATTC
  541 CTCTCGGGAC AGAAACAAGC CTTTTAAGTT TACACTAGGC AAGCAGGAGG TGATCCGAGG
  601 CTGGGAGGAA GGGGTAGCCC AGATGAGTGT GGGTCAGAGA GCCAAACTGA TAATCTCCTC
  661 AGACTATGCC TATGGAGCCA CCGGGCACCC AGGCATCATC CCACCACATG CCACTCTTGT
  721 TTTTGATGTG GAGCTTCTAA AACTGGAAGG AAGATCCGGA GTGCAGGTGG AGACCATCTC
  781 TCCTGGANAC GGGCGCACCT TCCCAAAGCG CGGCCAGACC TGCGTGGTGC ACTACACGGG
  841 GATGCTTGAA GANGGAAAGA AAATGGATTC CTCTCNGGNN AGAAACAAGC CTTTTANGTT
  901 TACACTNNNC AAGCANGANG TGATCCGANG CTNGGNNGNA NGGGGTANCC CNNNNGANTG
  961 TGGGTCANAN ANNCNNANCT GANANTNNNC NTCANANTAN NNNNATNGNN NCNNNNNGGN
1021 NNCNNNGGNN NNNNNNCCNN NNNNNNNN
//
Primer: IRES-R
Region: TMD
Sequence file: RDB17365_A9Lgc.seq check
>D06377A9_A9Lg_1_IRES-R_C10_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNCNGG NNNNNNNCGT NAGGGGGGGG GGGCGNNNNN GATCCCGCTA
   61 GAGGGAAACC GTTGCTAGCT TAAGTTAGTT AACAATTGTT ACCGCGGCCG CTTAGGATCC
  121 CCATAGAAGG AAGACCCCCA GGCAAAGGAA GACGAGGAGC ACGATGGGGA GGACAATGGA
  181 CAGAGCANAT CTGGAACCAC CGGTACCACC GGAACCACCC GTCGACCCTC CTTCCAGTTT
  241 TAGAAGCTCC ACATCAAAAA CAAGAGTGGC ATGTGGTGGG ATGATGCCTG GGTGCCCGGT
  301 GGCTCCATAG GCATAGTCTG AGGAGATTAT CAGTTTGGCT CTCTGACCCA CACTCATCTG
  361 GGCTACCCCT TCCTCCCAGC CTCGGATCAC CTCCTGCTTG CCTAGTGTAA ACTTAAAAGG
  421 CTTGTTTCTG TCCCGAGAGG AATCCATTTT CTTTCCATCT TCAAGCATCC CCGTGTAGTG
  481 CACCACGCAG GTCTGGCCGC GCTTTGGGAA GGTGCGCCCG TCTCCAGGAN AGATGGTCTC
  541 CACCTGCACT CCGGATCTTC CTTCCAGTTT TAGAAGCTCC ACATCAAAAA CAAGAGTGGC
  601 ATGTGGTGGG ATGATGCCTG GGTGCCCGGT GGCTCCATAG GCATAGTCTG AGGAGATTAT
  661 CAGTTTGGCT CTCTGACCCA CANNNNNNTG GGCTACCCCT TCCTCCCAGC CTCNNATCAC
  721 CTCCTGCTTG CCTAGTGTAA ACTTAAAAGG NTTGTTTCTG TCCCGAGAGG AATCCATTTT
  781 CTTTCCATCT TCAAGCATCC CCGTGTAGTG CACCACGCAN GTCTGGNCGC GCTTTGGGAA
  841 NNNGCGCCCG TCNCCAGGAN AGANGGTCTC CNCCTGCNCT CCNNNNCTNN CNTCNNGTTT
  901 TANAANCTCC ACATCAAAAA NNANNANTGN NATGNGGNGG GNNNANGNCN GGGNGNCCGG
  961 NNGNNTNCNT NNNNNTANTC NNNNGANNNT ATNNNNTTNN NNNNNNGANC CNNNNTCNTC
1021 TGGGNTACCC NNNNNNNCNN NCNNNNNNNN ANNNCNNNNN NNNNN
//
Primer: IRES-F
Region: IRES, bsr
Sequence file: RDB17365_A9Lgd.seq check
>D06377A9_A9Lg_1_IRES-F_C09_09_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNT TNNNAAAACG TCTAGGCCCC CCGAACCNCG
   61 GGGNCGTGGT TTTCCTTTGA AAAACACGAT AATACCATGG TCATGAAAAC ATTTAACATT
  121 TCTCAACAAG ATCTAGAATT AGTAGAAGTA GCGACAGAGA AGATTACAAT GCTTTATGAG
  181 GATAATAAAC ATCATGTGGG AGCGGCAATT CGTACGAAAA CAGGAGAAAT CATTTCGGCA
  241 GTACATATTG AAGCGTATAT AGGACGAGTA ACTGTTTGTG CAGAAGCCAT TGCGATTGGT
  301 AGTGCAGTTT CGAATGGACA AAAGGATTTT GACACGATTG TAGCTGTTAG ACACCCTTAT
  361 TCTGACGAAG TAGATAGAAG TATTCGAGTG GTAAGTCCTT GTGGTATGTG TAGGGAGTTG
  421 ATTTCAGACT ATGCACCAGA TTGTTTTGTG TTAATAGAAA TGAATGGCAA GTTAGTCAAA
  481 ACTACGATTG AAGAACTCAT TCCACTCAAA TATACCCGAA ATTAAAAGTC GACGATAAAA
  541 TAAAAGATTT TATTTAGTCT CCAGAAAAAG GGGGGAATGA AAGACCCCAC CTGTAGGTTT
  601 GGCAAGCTAG CTTAAGTAAC GCCATTTTGC AAGGCATGGA AAAATACATA ACTGAGAATA
  661 NAGAAGTTCA GATCAAGGTC AGGAACAGAT GGAACAGCTG AATATGGGCC AAACAGGATA
  721 TCTGTGGTAA GCAGTTCCTG CCCCGGCTCA NGGCCAAGAA CAGATGGAAC AGCTGAATAT
  781 GGGCCNAACA GGATATCTGT GGTAAGCAGT TCCTGCNNCG GCTCNNGGCC AAGAACAGAT
  841 GGTCCCCAGA TGCGGNCNNG CCCTCANCAG TTTCTAGAGA ANCNTCNNAT GTTTCCNGGG
  901 NGCCCCANNG ANCNGAAATG ANCCTGNGCC TTATTTGAAC TAACCNATCA GTTCGCTTCT
  961 CGCTNCTGTT NNCGNNNNNN NGNNNNNNNN CNCNNNAAAA GANCCNNCNA NCNNNNNNNN
1021 NN
//
Primer: IRESup_R1
Region: TMD, FM(4), FM(3)
Sequence file: RDB17365_A9Lge.seq check
>D06377A9_A9Lg_1_IRESup_R1_A11_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNCCGCGGCC GCTTAGGNNC CCCNTAGAAG
   61 GAAGACCCCC AGGCAAAGGA AGACGAGGAG CACGATGGGG AGGACAATGG ACAGAGCAGA
  121 TCTGGAACCA CCGGTACCAC CGGAACCACC CGTCGACCCT CCTTCCAGTT TTAGAAGCTC
  181 CACATCAAAA ACAAGAGTGG CATGTGGTGG GATGATGCCT GGGTGCCCGG TGGCTCCATA
  241 GGCATAGTCT GAGGAGATTA TCAGTTTGGC TCTCTGACCC ACACTCATCT GGGCTACCCC
  301 TTCCTCCCAG CCTCGGATCA CCTCCTGCTT GCCTAGTGTA AACTTAAAAG GCTTGTTTCT
  361 GTCCCGAGAG GAATCCATTT TCTTTCCATC TTCAAGCATC CCCGTGTAGT GCACCACGCA
  421 GGTCTGGCCG CGCTTTGGGA AGGTGCGCCC GTCTCCAGGA GAGATGGTCT CCACCTGCAC
  481 TCCGGATCTT CCTTCCAGTT TTAGAAGCTC CACATCAAAA ACAAGAGTGG CATGTGGTGG
  541 GATGATGCCT GGGTGCCCGG TGGCTCCATA GGCATAGTCT GAGGAGATTA TCAGTTTGGC
  601 TCTCTGACCC ACACTCATCT GGGCTACCCC TTCCTCCCAG CCTCGGATCA CCTCCTGCTT
  661 GCCTAGTGTA AACTTAAAAG GCTTGTTTCT GTCCCGAGAG GAATCCATTT TCTTTCCATC
  721 TTCAAGCATC CCCGTGTAGT GCACCACGCA GGTCTGGCCG CGCTTTGGGA AGGTGCGCCC
  781 GTCTCCAGGA NAGATGGTCT CCACCTGCAC TCCNGATCTT CCTTCCNGTT TTAGAAGCTC
  841 CACATCAAAA ACAAGAGTGN NATGTGGTGG GNNGANGCCT GGGTGCCCNG GTGGNTCCNN
  901 NNGCATAGTC TGAGGAGANT ATCNNTTTGG CTCTCTGANC CNCACTCATC NGGGCTACCC
  961 CTTCCTCCCN GCCNNGNANN ACNNNCTGCN NGNCNANNGN AAACTTAAAN NNNNNGNTTC
1021 TGNNCCNNNA NNNANNNNNT TNCTTTNNNT NNNNNNNNNT NCNCNNNNNN NNNN
//
Primer: CMV enh_F1
Region: CMV en, 5' LTR, MMLV psi
Sequence file: RDB17365_A9Lgf.seq check
>D06377A9_A9Lg_1_CMVenh_F1_D09_12_ABI24.ab1
    1 NNNNNNNNNN NNNNNNTGNN NNGNNNNNNN NCTTATGGGN NTTTCCTACT TGGCAGTACN
   61 NTCTACGTAT TAGTCATCGC TATTACCATG CTAGAGAACC ATCAGATGTT TCCAGGGTGC
  121 CCCAAGGACC TGAAATGACC CTGTGCCTTA TTTGAACTAA CCAATCAGTT CGCTTCTCGC
  181 TTCTGTTCGC GCGCTTCTGC TCCCCGAGCT CAATAAAAGA GCCCACAACC CCTCACTCGG
  241 CGCGCCAGTC CTCCGATTGA CTGAGTCGCC CGGGTACCCG TGTATCCAAT AAACCCTCTT
  301 GCAGTTGCAT CCGACTTGTG GTCTCGCTGT TCCTTGGGAG GGTCTCCTCT GAGTGATTGA
  361 CTACCCGTCA GCGGGGGTCT TTCATTTGGG GGCTCGTCCG GGATCGGGAG ACCCCTGCCC
  421 AGGGACCACC GACCCACCAC CGGGAGGTAA GCTGGCCAGC AACTTATCTG TGTCTGTCCG
  481 ATTGTCTAGT GTCTATGACT GATTTTATGC GCCTGCGTCG GTACTAGTTA GCTAACTAGC
  541 TCTGTATCTG GCGGACCCGT GGTGGAACTG ACGAGTTCGG AACACCCGGC CGCAACCCTG
  601 GGAGACGTCC CAGGGACTTC GGGGGCCGTT TTTGTGGCCC GACCTGAGTC CAAAAATCCC
  661 GATCGTTTTG GACTCTTTGG TGCACCCCCC TTAGAGGAGG GATATGTGGT TCTGGTAGGA
  721 GACGAGAACC TAAAACAGTT CCCGCCTCCG TCTGAATTTT TGCTTTCGGT TTGGGACCGA
  781 ANCCGCGCCG CGCGTCTTGT CTGCTGCAGC ATCGTTCTGT GTTGTCTCTG TCTGANTGTG
  841 TTTCTGTATT TGTCTGAAAA TATGGGCCCG GGCCAGANTG TTACCACTCC NNTAAGTTTG
  901 ANCTTNNNNC ANTGGAAAGA NGTCGAGCGG NTCGCTCNCN NCCNGNCNGT ANANGTCNAG
961 NNNANNNNNT GGNNNANCNT NNNGNNNNGN NNANNNNNCN NNNCNTTNNN NNNNNNN
//
Primer: bsr-C_F
Region: bsr, 3' LTR
Sequence file: RDB17365_A9Lgg.seq check
>D06377A9_A9Lg_1_bsr-C_F_E09_15_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNGT TTTGNGNNNN NGAANNGAAT GGCAAGTTAG TCAAAACTAC
   61 GATTGAAGAA NNCNTTCCAC TCAAATATAC CCGAAATTAA AAGTCGACGA TAAAATAAAA
  121 GATTTTATTT AGTCTCCAGA AAAAGGGGGG AATGAAAGAC CCCACCTGTA GGTTTGGCAA
  181 GCTAGCTTAA GTAACGCCAT TTTGCAAGGC ATGGAAAAAT ACATAACTGA GAATAGAGAA
  241 GTTCAGATCA AGGTCAGGAA CAGATGGAAC AGCTGAATAT GGGCCAAACA GGATATCTGT
  301 GGTAAGCAGT TCCTGCCCCG GCTCAGGGCC AAGAACAGAT GGAACAGCTG AATATGGGCC
  361 AAACAGGATA TCTGTGGTAA GCAGTTCCTG CCCCGGCTCA GGGCCAAGAA CAGATGGTCC
  421 CCAGATGCGG TCCAGCCCTC AGCAGTTTCT AGAGAACCAT CAGATGTTTC CAGGGTGCCC
  481 CAAGGACCTG AAATGACCCT GTGCCTTATT TGAACTAACC AATCAGTTCG CTTCTCGCTT
  541 CTGTTCGCGC GCTTCTGCTC CCCGAGCTCA ATAAAAGAGC CCACAACCCC TCACTCGGGG
  601 CGCCAGTCCT CCGATTGACT GAGTCGCCCG GGTACCCGTG TATCCAATAA ACCCTCTTGC
  661 AGTTGCATCC GACTTGTGGT CTCGCTGTTC CTTGGGAGGG TCTCCTCTGA GTGATTGACT
  721 ACCCGTCAGC GGGGGTCTTT CACATGCAGC ATGTATCAAA ATTAATTTGG TTTTTTTTCT
  781 TAAGTATTTA CATTAAATGG CCATAGTTGC ATTAATGAAT CGGCCAACGC GCGGGNNNAN
  841 GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCNCGCTCAC TGANTCGCTG CGCTCGGTCG
  901 TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAANNGNGGN AATACNGNNA TCCNCAGAAT
  961 CNGGGNNNAA CNCNNNNAAN NNNNNGNGNN CAAANNNNCA NNNNANNNNN NNGNNNNCNN
1021 NNAAANNNNC NNNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Homma, Y., Comprehensive knockout analysis of the Rab family GTPases in epithelial cells. J. Cell Biol. 218 (6): 2035-2050 (2019). PMID 31072826.
original Hirano, S., Differing susceptibility to autophagic degradation of two LC3-binding proteins: SQSTM1/p62 and TBC1D25/OATL1. Autophagy 12 (2): 312-326 (2016). PMID 26902585.

2020.07.02

GNP_filter3_RDBDEP_html_200316.pl