Retroviral expression vector enabling a synchronized trafficking assay.
Clone info. | Retroviral expression vector enabling a synchronized trafficking assay, EGFP tagged with the signal sequence from mouse calreticulin (ss) at its N-terminus and FM4-TMD at its C terminus. FM4, 4 x mouse FK506 binding protein 1a (FKBP1A/FKBP12) mutant (Phe-to-Met substitution at amino acid position 37). TMD, transmembrane domain from human low density lipoprotein receptor (LDLR). See Fig.5A of Homma, Y. et al. J. Cell Biol. 218 (6): 2035-2050, 2019. |
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Vector backbone | pMRX-IRES-bsr (plasmid) |
Selectable markers | Amp^r (E. coli), Blasticidin^r (mammalian cells). |
Gene/insert name | human LDLR cDNA mouse Calr cDNA mouse Fkbp1a cDNA |
Depositor|Developer | Fukuda, Mitsunori | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Hirano, S. et al. Autophagy 12, 312-326 (FM4), 2016); (Homma, Y. et al. J. Cell Biol., in press (doi: 10.1083/jcb.201810134), 2019) Additional terms and conditions: Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested. |
Remarks | Remember that you will be working with samples containing infectious virus. |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Hirano, S. et al. Autophagy 12, 312-326 (FM4), 2016); (Homma, Y. et al. J. Cell Biol., in press (doi: 10.1083/jcb.201810134), 2019)。 MTAに書く付加的使用条件: pMRXベクターについて:利用者は、研究成果の公表にあたって pMXならびにpMRXベクターの文献 (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) の引用を必要とする。 |
備考 | このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB17365 | pMRX-bsr-ss-EGFP-FM4-TMD | DNA solution |
JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge |
Materials & Methods section:
The pMRX-bsr-ss-EGFP-FM4-TMD was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17365). |
Reference section:
Homma, Y., Kinoshita, R., Kuchitsu, Y., Wawro, P.S., Marubashi, S., Oguchi, M.E., Ishida, M., Fujita, N., Fukuda, M., Comprehensive knockout analysis of the Rab family GTPases in epithelial cells. J. Cell Biol. 218 (6): 2035-2050 (2019). PMID 31072826. [link to RRC of NBRP] |
Hirano, S., Uemura, T., Annoh, H., Fujita, N., Waguri, S., Itoh, T., Fukuda, M., Differing susceptibility to autophagic degradation of two LC3-binding proteins: SQSTM1/p62 and TBC1D25/OATL1. Autophagy 12 (2): 312-326 (2016). PMID 26902585. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB17365_A9Lgp1-1.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: MMLV-pol_F (Pr0352) Region: pol region, SS, EGFP 5' Sequence file: RDB17365_A9Lga.seq ![]() |
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>D06377A9_A9Lg_1_MMLV-pol_F_A09_03_ABI24.ab1 1 NNNNNNNNNN NNNNNNNGGN ATCGCAGCTT GGNNACNCGC CGCCCACGTG AAGGCTGCCG 61 ACCCCGGGGG TGGACCATCC TCTAGACTGC CGGATCTGCC ACCATGCTCC TTTCGGTGCC 121 GCTCCTGCTT GGCCTCCTCG GCCTGGCCGC CGCAAGATCC ATGGTGAGCA AGGGCGAGGA 181 GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA 241 GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA CCCTGAAGTT 301 CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCCTGACCTA 361 CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC 421 CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG ACGGCAACTA 481 CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA 541 GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTACAA 601 CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC GGCATCAAGG TGAACTTCAA 661 GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC AGCAGAACAC 721 CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGANCA CCCAGTCCGC 781 CCTGNNCAAA GANCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGANT TCGTGACCGC 841 CGCCGGGNTC ACTCTCGGCA TGGACGANCT GTACAAGGGN TCCGGANNGC ANGTGGAGNN 901 CATCTCTNCN GNANNNNGGC GCANNTTNCC NANGCGCGGN CANANCNGCN NNGNNNCANT 961 ACNNNGGNNN NNNNNNN // |
Primer: EGFP-C2 (Pr0402) Region: EGFP 3', FM(1), FM(2), FM(3) Sequence file: RDB17365_A9Lgb.seq ![]() |
>D06377A9_A9Lg_1_EGFP-C2_F09_18_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN GGTCCTGCTG GAGTTCGTGN NCGCCGCCGG 61 GATCACTCTC GGCATGGNNN AGCTGTACAA GGGATCCGGA GTGCAGGTGG AGACCATCTC 121 TCCTGGAGAC GGGCGCACCT TCCCAAAGCG CGGCCAGACC TGCGTGGTGC ACTACACGGG 181 GATGCTTGAA GATGGAAAGA AAATGGATTC CTCTCGGGAC AGAAACAAGC CTTTTAAGTT 241 TACACTAGGC AAGCAGGAGG TGATCCGAGG CTGGGAGGAA GGGGTAGCCC AGATGAGTGT 301 GGGTCAGAGA GCCAAACTGA TAATCTCCTC AGACTATGCC TATGGAGCCA CCGGGCACCC 361 AGGCATCATC CCACCACATG CCACTCTTGT TTTTGATGTG GAGCTTCTAA AACTGGAAGG 421 AAGATCCGGA GTGCAGGTGG AGACCATCTC TCCTGGAGAC GGGCGCACCT TCCCAAAGCG 481 CGGCCAGACC TGCGTGGTGC ACTACACGGG GATGCTTGAA GATGGAAAGA AAATGGATTC 541 CTCTCGGGAC AGAAACAAGC CTTTTAAGTT TACACTAGGC AAGCAGGAGG TGATCCGAGG 601 CTGGGAGGAA GGGGTAGCCC AGATGAGTGT GGGTCAGAGA GCCAAACTGA TAATCTCCTC 661 AGACTATGCC TATGGAGCCA CCGGGCACCC AGGCATCATC CCACCACATG CCACTCTTGT 721 TTTTGATGTG GAGCTTCTAA AACTGGAAGG AAGATCCGGA GTGCAGGTGG AGACCATCTC 781 TCCTGGANAC GGGCGCACCT TCCCAAAGCG CGGCCAGACC TGCGTGGTGC ACTACACGGG 841 GATGCTTGAA GANGGAAAGA AAATGGATTC CTCTCNGGNN AGAAACAAGC CTTTTANGTT 901 TACACTNNNC AAGCANGANG TGATCCGANG CTNGGNNGNA NGGGGTANCC CNNNNGANTG 961 TGGGTCANAN ANNCNNANCT GANANTNNNC NTCANANTAN NNNNATNGNN NCNNNNNGGN 1021 NNCNNNGGNN NNNNNNCCNN NNNNNNNN // |
Primer: IRES-R (Pr0037) Region: TMD Sequence file: RDB17365_A9Lgc.seq ![]() |
>D06377A9_A9Lg_1_IRES-R_C10_03_ABI08.ab1 1 NNNNNNNNNN NNNNNNCNGG NNNNNNNCGT NAGGGGGGGG GGGCGNNNNN GATCCCGCTA 61 GAGGGAAACC GTTGCTAGCT TAAGTTAGTT AACAATTGTT ACCGCGGCCG CTTAGGATCC 121 CCATAGAAGG AAGACCCCCA GGCAAAGGAA GACGAGGAGC ACGATGGGGA GGACAATGGA 181 CAGAGCANAT CTGGAACCAC CGGTACCACC GGAACCACCC GTCGACCCTC CTTCCAGTTT 241 TAGAAGCTCC ACATCAAAAA CAAGAGTGGC ATGTGGTGGG ATGATGCCTG GGTGCCCGGT 301 GGCTCCATAG GCATAGTCTG AGGAGATTAT CAGTTTGGCT CTCTGACCCA CACTCATCTG 361 GGCTACCCCT TCCTCCCAGC CTCGGATCAC CTCCTGCTTG CCTAGTGTAA ACTTAAAAGG 421 CTTGTTTCTG TCCCGAGAGG AATCCATTTT CTTTCCATCT TCAAGCATCC CCGTGTAGTG 481 CACCACGCAG GTCTGGCCGC GCTTTGGGAA GGTGCGCCCG TCTCCAGGAN AGATGGTCTC 541 CACCTGCACT CCGGATCTTC CTTCCAGTTT TAGAAGCTCC ACATCAAAAA CAAGAGTGGC 601 ATGTGGTGGG ATGATGCCTG GGTGCCCGGT GGCTCCATAG GCATAGTCTG AGGAGATTAT 661 CAGTTTGGCT CTCTGACCCA CANNNNNNTG GGCTACCCCT TCCTCCCAGC CTCNNATCAC 721 CTCCTGCTTG CCTAGTGTAA ACTTAAAAGG NTTGTTTCTG TCCCGAGAGG AATCCATTTT 781 CTTTCCATCT TCAAGCATCC CCGTGTAGTG CACCACGCAN GTCTGGNCGC GCTTTGGGAA 841 NNNGCGCCCG TCNCCAGGAN AGANGGTCTC CNCCTGCNCT CCNNNNCTNN CNTCNNGTTT 901 TANAANCTCC ACATCAAAAA NNANNANTGN NATGNGGNGG GNNNANGNCN GGGNGNCCGG 961 NNGNNTNCNT NNNNNTANTC NNNNGANNNT ATNNNNTTNN NNNNNNGANC CNNNNTCNTC 1021 TGGGNTACCC NNNNNNNCNN NCNNNNNNNN ANNNCNNNNN NNNNN // |
Primer: IRES-F (Pr0036) Region: IRES, bsr Sequence file: RDB17365_A9Lgd.seq ![]() |
>D06377A9_A9Lg_1_IRES-F_C09_09_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNT TNNNAAAACG TCTAGGCCCC CCGAACCNCG 61 GGGNCGTGGT TTTCCTTTGA AAAACACGAT AATACCATGG TCATGAAAAC ATTTAACATT 121 TCTCAACAAG ATCTAGAATT AGTAGAAGTA GCGACAGAGA AGATTACAAT GCTTTATGAG 181 GATAATAAAC ATCATGTGGG AGCGGCAATT CGTACGAAAA CAGGAGAAAT CATTTCGGCA 241 GTACATATTG AAGCGTATAT AGGACGAGTA ACTGTTTGTG CAGAAGCCAT TGCGATTGGT 301 AGTGCAGTTT CGAATGGACA AAAGGATTTT GACACGATTG TAGCTGTTAG ACACCCTTAT 361 TCTGACGAAG TAGATAGAAG TATTCGAGTG GTAAGTCCTT GTGGTATGTG TAGGGAGTTG 421 ATTTCAGACT ATGCACCAGA TTGTTTTGTG TTAATAGAAA TGAATGGCAA GTTAGTCAAA 481 ACTACGATTG AAGAACTCAT TCCACTCAAA TATACCCGAA ATTAAAAGTC GACGATAAAA 541 TAAAAGATTT TATTTAGTCT CCAGAAAAAG GGGGGAATGA AAGACCCCAC CTGTAGGTTT 601 GGCAAGCTAG CTTAAGTAAC GCCATTTTGC AAGGCATGGA AAAATACATA ACTGAGAATA 661 NAGAAGTTCA GATCAAGGTC AGGAACAGAT GGAACAGCTG AATATGGGCC AAACAGGATA 721 TCTGTGGTAA GCAGTTCCTG CCCCGGCTCA NGGCCAAGAA CAGATGGAAC AGCTGAATAT 781 GGGCCNAACA GGATATCTGT GGTAAGCAGT TCCTGCNNCG GCTCNNGGCC AAGAACAGAT 841 GGTCCCCAGA TGCGGNCNNG CCCTCANCAG TTTCTAGAGA ANCNTCNNAT GTTTCCNGGG 901 NGCCCCANNG ANCNGAAATG ANCCTGNGCC TTATTTGAAC TAACCNATCA GTTCGCTTCT 961 CGCTNCTGTT NNCGNNNNNN NGNNNNNNNN CNCNNNAAAA GANCCNNCNA NCNNNNNNNN 1021 NN // |
Primer: IRESup_R1 (Pr0804) Region: TMD, FM(4), FM(3) Sequence file: RDB17365_A9Lge.seq ![]() |
>D06377A9_A9Lg_1_IRESup_R1_A11_01_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNCCGCGGCC GCTTAGGNNC CCCNTAGAAG 61 GAAGACCCCC AGGCAAAGGA AGACGAGGAG CACGATGGGG AGGACAATGG ACAGAGCAGA 121 TCTGGAACCA CCGGTACCAC CGGAACCACC CGTCGACCCT CCTTCCAGTT TTAGAAGCTC 181 CACATCAAAA ACAAGAGTGG CATGTGGTGG GATGATGCCT GGGTGCCCGG TGGCTCCATA 241 GGCATAGTCT GAGGAGATTA TCAGTTTGGC TCTCTGACCC ACACTCATCT GGGCTACCCC 301 TTCCTCCCAG CCTCGGATCA CCTCCTGCTT GCCTAGTGTA AACTTAAAAG GCTTGTTTCT 361 GTCCCGAGAG GAATCCATTT TCTTTCCATC TTCAAGCATC CCCGTGTAGT GCACCACGCA 421 GGTCTGGCCG CGCTTTGGGA AGGTGCGCCC GTCTCCAGGA GAGATGGTCT CCACCTGCAC 481 TCCGGATCTT CCTTCCAGTT TTAGAAGCTC CACATCAAAA ACAAGAGTGG CATGTGGTGG 541 GATGATGCCT GGGTGCCCGG TGGCTCCATA GGCATAGTCT GAGGAGATTA TCAGTTTGGC 601 TCTCTGACCC ACACTCATCT GGGCTACCCC TTCCTCCCAG CCTCGGATCA CCTCCTGCTT 661 GCCTAGTGTA AACTTAAAAG GCTTGTTTCT GTCCCGAGAG GAATCCATTT TCTTTCCATC 721 TTCAAGCATC CCCGTGTAGT GCACCACGCA GGTCTGGCCG CGCTTTGGGA AGGTGCGCCC 781 GTCTCCAGGA NAGATGGTCT CCACCTGCAC TCCNGATCTT CCTTCCNGTT TTAGAAGCTC 841 CACATCAAAA ACAAGAGTGN NATGTGGTGG GNNGANGCCT GGGTGCCCNG GTGGNTCCNN 901 NNGCATAGTC TGAGGAGANT ATCNNTTTGG CTCTCTGANC CNCACTCATC NGGGCTACCC 961 CTTCCTCCCN GCCNNGNANN ACNNNCTGCN NGNCNANNGN AAACTTAAAN NNNNNGNTTC 1021 TGNNCCNNNA NNNANNNNNT TNCTTTNNNT NNNNNNNNNT NCNCNNNNNN NNNN // |
Primer: CMV enh_F1 (Pr0652) Region: CMV en, 5' LTR, MMLV psi Sequence file: RDB17365_A9Lgf.seq ![]() |
>D06377A9_A9Lg_1_CMVenh_F1_D09_12_ABI24.ab1 1 NNNNNNNNNN NNNNNNTGNN NNGNNNNNNN NCTTATGGGN NTTTCCTACT TGGCAGTACN 61 NTCTACGTAT TAGTCATCGC TATTACCATG CTAGAGAACC ATCAGATGTT TCCAGGGTGC 121 CCCAAGGACC TGAAATGACC CTGTGCCTTA TTTGAACTAA CCAATCAGTT CGCTTCTCGC 181 TTCTGTTCGC GCGCTTCTGC TCCCCGAGCT CAATAAAAGA GCCCACAACC CCTCACTCGG 241 CGCGCCAGTC CTCCGATTGA CTGAGTCGCC CGGGTACCCG TGTATCCAAT AAACCCTCTT 301 GCAGTTGCAT CCGACTTGTG GTCTCGCTGT TCCTTGGGAG GGTCTCCTCT GAGTGATTGA 361 CTACCCGTCA GCGGGGGTCT TTCATTTGGG GGCTCGTCCG GGATCGGGAG ACCCCTGCCC 421 AGGGACCACC GACCCACCAC CGGGAGGTAA GCTGGCCAGC AACTTATCTG TGTCTGTCCG 481 ATTGTCTAGT GTCTATGACT GATTTTATGC GCCTGCGTCG GTACTAGTTA GCTAACTAGC 541 TCTGTATCTG GCGGACCCGT GGTGGAACTG ACGAGTTCGG AACACCCGGC CGCAACCCTG 601 GGAGACGTCC CAGGGACTTC GGGGGCCGTT TTTGTGGCCC GACCTGAGTC CAAAAATCCC 661 GATCGTTTTG GACTCTTTGG TGCACCCCCC TTAGAGGAGG GATATGTGGT TCTGGTAGGA 721 GACGAGAACC TAAAACAGTT CCCGCCTCCG TCTGAATTTT TGCTTTCGGT TTGGGACCGA 781 ANCCGCGCCG CGCGTCTTGT CTGCTGCAGC ATCGTTCTGT GTTGTCTCTG TCTGANTGTG 841 TTTCTGTATT TGTCTGAAAA TATGGGCCCG GGCCAGANTG TTACCACTCC NNTAAGTTTG 901 ANCTTNNNNC ANTGGAAAGA NGTCGAGCGG NTCGCTCNCN NCCNGNCNGT ANANGTCNAG 961 NNNANNNNNT GGNNNANCNT NNNGNNNNGN NNANNNNNCN NNNCNTTNNN NNNNNNN // |
Primer: bsr-C_F (Pr0803) Region: bsr, 3' LTR Sequence file: RDB17365_A9Lgg.seq ![]() |
>D06377A9_A9Lg_1_bsr-C_F_E09_15_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNGT TTTGNGNNNN NGAANNGAAT GGCAAGTTAG TCAAAACTAC 61 GATTGAAGAA NNCNTTCCAC TCAAATATAC CCGAAATTAA AAGTCGACGA TAAAATAAAA 121 GATTTTATTT AGTCTCCAGA AAAAGGGGGG AATGAAAGAC CCCACCTGTA GGTTTGGCAA 181 GCTAGCTTAA GTAACGCCAT TTTGCAAGGC ATGGAAAAAT ACATAACTGA GAATAGAGAA 241 GTTCAGATCA AGGTCAGGAA CAGATGGAAC AGCTGAATAT GGGCCAAACA GGATATCTGT 301 GGTAAGCAGT TCCTGCCCCG GCTCAGGGCC AAGAACAGAT GGAACAGCTG AATATGGGCC 361 AAACAGGATA TCTGTGGTAA GCAGTTCCTG CCCCGGCTCA GGGCCAAGAA CAGATGGTCC 421 CCAGATGCGG TCCAGCCCTC AGCAGTTTCT AGAGAACCAT CAGATGTTTC CAGGGTGCCC 481 CAAGGACCTG AAATGACCCT GTGCCTTATT TGAACTAACC AATCAGTTCG CTTCTCGCTT 541 CTGTTCGCGC GCTTCTGCTC CCCGAGCTCA ATAAAAGAGC CCACAACCCC TCACTCGGGG 601 CGCCAGTCCT CCGATTGACT GAGTCGCCCG GGTACCCGTG TATCCAATAA ACCCTCTTGC 661 AGTTGCATCC GACTTGTGGT CTCGCTGTTC CTTGGGAGGG TCTCCTCTGA GTGATTGACT 721 ACCCGTCAGC GGGGGTCTTT CACATGCAGC ATGTATCAAA ATTAATTTGG TTTTTTTTCT 781 TAAGTATTTA CATTAAATGG CCATAGTTGC ATTAATGAAT CGGCCAACGC GCGGGNNNAN 841 GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCNCGCTCAC TGANTCGCTG CGCTCGGTCG 901 TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAANNGNGGN AATACNGNNA TCCNCAGAAT 961 CNGGGNNNAA CNCNNNNAAN NNNNNGNGNN CAAANNNNCA NNNNANNNNN NNGNNNNCNN 1021 NNAAANNNNC NNNNNNNNNN // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Homma, Y., Comprehensive knockout analysis of the Rab family GTPases in epithelial cells. J. Cell Biol. 218 (6): 2035-2050 (2019). PMID 31072826. [link to RRC of NBRP] |
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original | Hirano, S., Differing susceptibility to autophagic degradation of two LC3-binding proteins: SQSTM1/p62 and TBC1D25/OATL1. Autophagy 12 (2): 312-326 (2016). PMID 26902585. [link to RRC of NBRP] |
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