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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMRX-bsr-ss-EGFP-FM4 (#RDB17364)

Retroviral expression vector of secretory form of EGFP fused with a conditional aggregation domain.

Clone info. Retroviral expression vector of EGFP tagged with the signal sequence from mouse calreticulin (ss) at its N-terminus and FM4 at its C terminus. FM4, 4 x mouse FK506 binding protein 1a (FKBP1A/FKBP12) mutant (Phe-to-Met substitution at amino acid position 37). See Fig. S4 of Hirano, S. et al., Autophagy, 12 (2):312-326, 2016.
Vector backbone pMRX-IRES-bsr (plasmid)
Selectable markers Amp^r (E. coli), Blasticidin^r (mammalian cells).
Gene/insert name mouse Calr cDNA mouse Fkbp1a cDNA
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Hirano, S. et al. Autophagy 12, 312-326 (FM4), 2016); (Homma, Y. et al. J. Cell Biol., in press (doi: 10.1083/jcb.201810134), 2019)
Additional terms and conditions:
Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested.
Remarks Remember that you will be working with samples containing infectious virus.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Hirano, S. et al. Autophagy 12, 312-326 (FM4), 2016); (Homma, Y. et al. J. Cell Biol., in press (doi: 10.1083/jcb.201810134), 2019)。
MTAに書く付加的使用条件：
pMRXベクターについて：利用者は、研究成果の公表にあたって pMXならびにpMRXベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) の引用を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17364 pMRX-bsr-ss-EGFP-FM4 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMRX-bsr-ss-EGFP-FM4 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17364).

Reference section:

Homma, Y., Kinoshita, R., Kuchitsu, Y., Wawro, P.S., Marubashi, S., Oguchi, M.E., Ishida, M., Fujita, N., Fukuda, M., Comprehensive knockout analysis of the Rab family GTPases in epithelial cells. J. Cell Biol. 218 (6): 2035-2050 (2019). PMID 31072826. [link to RRC of NBRP]
Hirano, S., Uemura, T., Annoh, H., Fujita, N., Waguri, S., Itoh, T., Fukuda, M., Differing susceptibility to autophagic degradation of two LC3-binding proteins: SQSTM1/p62 and TBC1D25/OATL1. Autophagy 12 (2): 312-326 (2016). PMID 26902585. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17364_A9Lgp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: MMLV-pol_F (Pr0352)
Region: pol region, SS, EGFP 5'
Sequence file: RDB17364_A9Lga.seq check
>D06377A8_A9Lg_1_MMLV-pol_F_A08_02_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNG GNATCGCAGC TTGGNNACNC GCCGCCCACG TGAAGGCTGC
   61 CGACCCCGGG GGTGGACCAT CCTCTAGACT GCCGGATCTG CCACCATGCT CCTTTCGGTG
  121 CCGCTCCTGC TTGGCCTCCT CGGCCTGGCC GCCGCAAGAT CCATGGTGAG CAAGGGCGAG
  181 GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT AAACGGCCAC
  241 AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT GACCCTGAAG
  301 TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC CACCCTGACC
  361 TACGGCGTGC AGTGCTTCAG CCGCTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG
  421 TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA CGACGGCAAC
  481 TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG
  541 AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA GTACAACTAC
  601 AACAGCCACA ACGTCTATAT CATGGCCGAC AAGCAGAAGA ACGGCATCAA GGTGAACTTC
  661 AAGATCCGCC ACAACATCGA GGACGGCAGC GTGCAGCTCG CCGACCACTA CCAGCAGAAC
  721 ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAN CACCCAGTCC
  781 GCCCTGANCA AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA NTTCGTGACC
  841 GCCGCCGGGN TCACTCTCGG CATGGACGAN CTGTACAANG GATCNNGANN GCANGNGGAG
  901 ANNNTCTCNN CTGGANNNGG GNGCACCNTN NCNNAAGCGC GGNCAGANNN GCNNNNNNCA
961 CTACNCGGGN NNGNNNNNNN NNGGNAAGAA ANNNNNNNNN N
//
Primer: EGFP-C2 (Pr0402)
Region: EGFP 3', FM(1), FM(2), FM(3)
Sequence file: RDB17364_A9Lgb.seq check
>D06377A8_A9Lg_1_EGFP-C2_F08_17_ABI24.ab1
    1 NNNNNNNNNN NNNNNCNNGN NCNCNTGGTC CTGCTGGANT TCGTGNCCGC CGCCGGGNTC
   61 ACTCTCGGCA TGGANGAGCT GTACAAGGGA TCCGGAGTGC AGGTGGAGAC CATCTCTCCT
  121 GGAGACGGGC GCACCTTCCC AAAGCGCGGC CAGACCTGCG TGGTGCACTA CACGGGGATG
  181 CTTGAAGATG GAAAGAAAAT GGATTCCTCT CGGGACAGAA ACAAGCCTTT TAAGTTTACA
  241 CTAGGCAAGC AGGAGGTGAT CCGAGGCTGG GAGGAAGGGG TAGCCCAGAT GAGTGTGGGT
  301 CAGAGAGCCA AACTGATAAT CTCCTCAGAC TATGCCTATG GAGCCACCGG GCACCCAGGC
  361 ATCATCCCAC CACATGCCAC TCTTGTTTTT GATGTGGAGC TTCTAAAACT GGAAGGAAGA
  421 TCCGGAGTGC AGGTGGAGAC CATCTCTCCT GGAGACGGGC GCACCTTCCC AAAGCGCGGC
  481 CAGACCTGCG TGGTGCACTA CACGGGGATG CTTGAAGATG GAAAGAAAAT GGATTCCTCT
  541 CGGGACAGAA ACAAGCCTTT TAAGTTTACA CTAGGCAAGC AGGAGGTGAT CCGAGGCTGG
  601 GAGGAAGGGG TAGCCCAGAT GAGTGTGGGT CAGAGAGCCA AACTGATAAT CTCCTCAGAC
  661 TATGCCTATG GAGCCACCGG GCACCCAGGC ATCATCCCAC CACATGCCAC TCTTGTTTTT
  721 GATGTGGAGC TTCTAAAACT GGAAGGAAGA TCCGGANTGC AGGTGGAGAN CATCTCTCCT
  781 GGAGACGGGC GCACCTTCCC NAAGCGCGGC CAGANCTGCG TGGTGCACTA CACGGGGNNG
  841 CTTGAAGATG GAAAGAAAAT GGATTCCTCT CGGGACAGAA ACAAGCCTTT TAAGTTTACA
  901 CTANGNNAGC AGGANGNGAT NCNANGNTGG GANGNNNGGG NANCCCAGAT GANTGNGGNN
  961 CANANANCCA AANTGANNNN CTNCNNCNNN NTATGNNNNT GGNNNNNNNN GNNNNNNNNN
1021 N
//
Primer: IRES-R (Pr0037)
Region: FM(4)
Sequence file: RDB17364_A9Lgc.seq check
>D06377A8_A9Lg_1_IRES-R_B08_05_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN ANGTNAGGGG GGGGGGGCGG AATTGNTCCC GCTAGAGGGA
   61 AACCGTTGCT AGCTTAAGTT AGTTAACAAT TGTTACCGCG GCCGCGAATT CACTAGTGAT
  121 TCTACGTCGA CCCTCCTTCC AGTTTTAGAA GCTCCACATC AAAAACAAGA GTGGCATGTG
  181 GTGGGATGAT GCCTGGGTGC CCGGTGGCTC CATAGGCATA GTCTGAGGAG ATTATCAGTT
  241 TGGCTCTCTG ACCCACACTC ATCTGGGCTA CCCCTTCCTC CCAGCCTCGG ATCACCTCCT
  301 GCTTGCCTAG NGTAAACTTA AAAGGCTTGT TTCTGTCCCG AGAGGAATCC ATTTTCTTTC
  361 CATCTTCAAG CATCCCCGTG TAGTGCACCA CGCAGGTCTG GCCGCGCTTT GGGAAGGTGC
  421 GCCCGTCTCC AGGANANATG GTCTCCACCT GCACTCCGGA TCTTCCTTCC AGTTTTANAA
  481 GCTCCACATC AAAAACAANA GTGGCATGTG GTGGGATGAT GCCTGGGTGC CCGGTGGCTC
  541 CATAGGCATA GTCTGAGGAG ATTATCAGTT TGGCTCTCTG ACCCACACTC ATCTGGGCTA
  601 CCCCTTCCTC CCANCCTCGG ATCACCTCCT GCTTGCCTAG TGTAAACTTA AAAGGCTTGT
  661 TTCTGTCCCG AGAGGAATCC ATTTTCTTTC CATCTTCAAG CATCCCCGTG TAGTGCACCA
  721 CGCAGGTCTG GCCGCGCTTT GGGAANGTGC GCCCGTCTCC AGGANAGANG GTCTCCACCT
  781 GCACTCCGGA TCTTCCTTCC AGTTTTANAA GCTCCACATC AAAAACAAGA NNGGCATGTG
  841 NNGGGNNGAN GCCNGGGNGC CCGGNGGCTC CNTAGGCNTA GNCNGNNGAN ATTATCAGTT
  901 TGNNTCTCTG ANCCACANTC NTCNGGGCTA CCCNNNCNNN CCNNNNNCNN NTNNNNNNCN
  961 GCTTGCNNAN NGNAAACTNN AAANNNNNTN NNTNNTGTCC NNNNNNGNNN NNNNNTTNNN
1021 TTNNNNNNNN NN
//
Primer: IRES-F (Pr0036)
Region: IRES, bsr, 3' LTR
Sequence file: RDB17364_A9Lgd.seq check
>D06377A8_A9Lg_1_IRES-F_C08_08_ABI24.ab1
    1 NNNNNNNNNN NNNTNNGTCG AGGNNNNNAA ACGTCTAGGC CCCCCGAACC ACGGGGANGT
   61 GGTTTTCCTT TGAAAAACAC GATAATACCA TGGTCATGAA AACATTTAAC ATTTCTCAAC
  121 AAGATCTAGA ATTAGTAGAA GTAGCGACAG AGAAGATTAC AATGCTTTAT GAGGATAATA
  181 AACATCATGT GGGAGCGGCA ATTCGTACGA AAACAGGAGA AATCATTTCG GCAGTACATA
  241 TTGAAGCGTA TATAGGACGA GTAACTGTTT GTGCAGAAGC CATTGCGATT GGTAGTGCAG
  301 TTTCGAATGG ACAAAAGGAT TTTGACACGA TTGTAGCTGT TAGACACCCT TATTCTGACG
  361 AAGTAGATAG AAGTATTCGA GTGGTAAGTC CTTGTGGTAT GTGTAGGGAG TTGATTTCAG
  421 ACTATGCACC AGATTGTTTT GTGTTAATAG AAATGAATGG CAAGTTAGTC AAAACTACGA
  481 TTGAAGAACT CATTCCACTC AAATATACCC GAAATTAAAA GTCGACGATA AAATAAAAGA
  541 TTTTATTTAG TCTCCAGAAA AAGGGGGGAA TGAAAGACCC CACCTGTAGG TTTGGCAAGC
  601 TAGCTTAAGT AACGCCATTT TGCAAGGCAT GGAAAAATAC ATAACTGAGA ATAGAGAAGT
  661 TCAGATCAAG GTCAGGAACA GATGGAACAG CTGAATATGG GCCAAACAGG ATATCTGTGG
  721 TAAGCAGTTC CTGCCCCGGC TCANGGCCAA GAACAGATGG AACAGCTGAA TATGGGCCAA
  781 ACAGGATATC TGTGGTAAGC AGTTCCTGNN NNGGCTCANG GCCNAGAACA GATGGTCNNC
  841 AGATGCGGTC CAGCCCTCAN CAGTTTCTAG AGAANCNTCA GANGTTTNCN GGGNGNCCCA
  901 AGGANNNNAA ATGANCNNGN GCCTTATTTG AACNNNNCNA TCANTTCGCT TCNCGCTNCN
961 GNNCGCGNGC TNNNGNNNCC NNANCTNNNN AAAAGANCNN NNNNNNNN
//
Primer: CMV enh_F1 (Pr0652)
Region: CMV en, 5' LTR, MMLV psi
Sequence file: RDB17364_A9Lge.seq check
>D06377A8_A9Lg_1_CMVenh_F1_D08_11_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNTGNNC TTATGGGNNT TTCCTACTTG GCAGNNNNNN
   61 TACGTATTAG TCATCGCTAT TACCATGCTA GAGAACCATC AGATGTTTCC AGGGTGCCCC
  121 AAGGACCTGA AATGACCCTG TGCCTTATTT GAACTAACCA ATCAGTTCGC TTCTCGCTTC
  181 TGTTCGCGCG CTTCTGCTCC CCGAGCTCAA TAAAAGAGCC CACAACCCCT CACTCGGCGC
  241 GCCAGTCCTC CGATTGACTG AGTCGCCCGG GTACCCGTGT ATCCAATAAA CCCTCTTGCA
  301 GTTGCATCCG ACTTGTGGTC TCGCTGTTCC TTGGGAGGGT CTCCTCTGAG TGATTGACTA
  361 CCCGTCAGCG GGGGTCTTTC ATTTGGGGGC TCGTCCGGGA TCGGGAGACC CCTGCCCAGG
  421 GACCACCGAC CCACCACCGG GAGGTAAGCT GGCCAGCAAC TTATCTGTGT CTGTCCGANT
  481 GTCTAGTGTC TATGACTGAT TTTATGCGCC TGCGTCNGTA CTAGTTAGCT NACTAGCTCT
  541 GTATCTGGCG GANCCNNGGN GNAACTGANN ANTNNNGAAC ACCCGGNNNN NACCCTGGGA
  601 NANNTCCCNN GNNNNNNNGG GNNNNNTTTT NNGNNCCNAN NNNNNTNNNA AANNCNNATN
661 NNTTNNNNNT NNTNNNNGNN CCCCCNNNNN NNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Homma, Y., Comprehensive knockout analysis of the Rab family GTPases in epithelial cells. J. Cell Biol. 218 (6): 2035-2050 (2019). PMID 31072826. [link to RRC of NBRP]
original Hirano, S., Differing susceptibility to autophagic degradation of two LC3-binding proteins: SQSTM1/p62 and TBC1D25/OATL1. Autophagy 12 (2): 312-326 (2016). PMID 26902585. [link to RRC of NBRP]