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pEGFP-C1-Rab27B (#RDB17274)

Expression vector of mouse Rab27B fused with EGFP.

Clone info. Expression vector of mouse Rab27B fused with EGFP at N-terminus.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cell).
Gene/insert name mouse Rab27b cDNA
Reference of insert sequence AB232623 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

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Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Fukuda, M. et al. (2002) J. Biol. Chem. 277, 12432-12436); (Matsui, T. et al. (2011) Traffic 12, 1432-1443)
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Fukuda, M. et al. (2002) J. Biol. Chem. 277, 12432-12436); (Matsui, T. et al. (2011) Traffic 12, 1432-1443)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17274 pEGFP-C1-Rab27B DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-Rab27B was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17274).

Reference section:

Matsui, T., Itoh, T., Fukuda, M., Small GTPase Rab12 regulates constitutive degradation of transferrin receptor. Traffic 12 (10): 1432-1443 (2011). PMID 21718402. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17274_A9Elp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 (Pr0402)
Region: EGFP 3', insert 5'
Sequence file: RDB17274_A9Ela.seq check
>D06280G2_A9El_2_EGFP-C2_A06_03_ABI24.ab1
    1 NNNNNNNNNN NNNNNNCGNN CNCNTGGTCC TGCTGGNNTT CGTGNNCGCC GCCGGGATCA
   61 CTCTCGGCAT GGACGAGCTG TACAAGTCCG GACTCAGATC CATGACTGAT GGAGACTATG
  121 ATTATCTGAT TAAACTCCTG GCCCTTGGAG ACTCAGGAGT CGGGAAGACA ACATTTCTCT
  181 ACAGATACAC AGACAATAAA TTCAATCCCA AATTCATCAC CACAGTGGGA ATAGATTTTC
  241 GGGAAAAACG TGTGGTTTAT GACACACAAG GAGCAGATGG AGCGTCAGGA AAAGCGTTTA
  301 AGGTACATCT GCAGCTTTGG GACACTGCTG GACAAGAGCG GTTCCGGAGC CTTACCACTG
  361 CCTTCTTCAG AGATGCCATG GGCTTCTTAC TGATGTTTGA CCTCACCAGT CAACAGAGCT
  421 TCTTGAATGT CAGAAACTGG ATGAGTCAAC TGCAGGCAAA TGCTTACTGT GAAAACCCAG
  481 ACATAGTATT AATTGGCAAC AAGGCAGACC TGCCAGACCA AAGGGAAGTC AATGAACGGC
  541 AAGCACGGGA GCTGGCTGAA AAATATGGCA TACCATACTT CGAAACAAGC GCAGCTACAG
  601 GACAGAATGT AGAGAAGTCA GTGGAAACGC TTCTGGACTT AATCATGAAG AGAATGGAAA
  661 AGTGTGTAGA GAAGACACAG GTTCCGGACA CTGTCAATGG ANGAAATTCT GGANAGCTGG
  721 ATGGGGANNA GCCAGCAGAA AAGAAATGTG CCTGCTAGAA TCACTAGTGA ATTCTGCAGT
  781 CGANGGTACC GCGGGCCCGG GNNCCACCGG NTCTAGATAA CNGATCATAA TCAGCCNTAC
  841 CACATTTGTA GANGTTTTAC TNGCTTTNAA AANNNNNNNN NCNNNTCCNN NNNNNANNNN
901 NNAANNTNAA ATGNANNGNN NNNNNNNN
//
Primer: SV40polyA-R2 (Pr0623)
Region: insert 3'
Sequence file: RDB17274_A9Elb.seq check
>D06280G2_A9El_2_SV40polyA-R2_F03_18_ABI24.ab1
    1 NNNNNNNNNN NNNNNNAANN NGTTAACNNC NACNATTGCA TTCATTTTAT GTTTCAGGTT
   61 CAGGGGGAGG TGTGGGAGGT TTTTTAAAGC AAGTAAAACC TCTACAAATG TGGTATGGCT
  121 GATTATGATC AGTTATCTAG ATCCGGTGGA TCCCGGGCCC GCGGTACCGT CGACTGCAGA
  181 ATTCACTAGT GATTCTAGCA GGCACATTTC TTTTCTGCTG GCTTTTCCCC ATCCAGCTTT
  241 CCAGAATTTC CTCCATTGAC AGTGTCCGGA ACCTGTGTCT TCTCTACACA CTTTTCCATT
  301 CTCTTCATGA TTAAGTCCAG AAGCGTTTCC ACTGACTTCT CTACATTCTG TCCTGTAGCT
  361 GCGCTTGTTT CGAAGTATGG TATGCCATAT TTTTCAGCCA GCTCCCGTGC TTGCCGTTCA
  421 TTGACTTCCC TTTGGTCTGG CAGGTCTGCC TTGTTGCCAA TTAATACTAT GTCTGGGTTT
  481 TCACAGTAAG CATTTGCCTG CAGTTGACTC ATCCAGTTTC TGACATTCAA GAAGCTCTGT
  541 TGACTGGTGA GGTCAAACAT CAGTAAGAAG CCCATGGCAT CTCTGAAGAA GGCAGTGGTA
  601 AGGCTCCGGA ACCGCTCTTG TCCAGCAGTG TCCCAAAGCT GCAGATGTAC CTTAAACGCT
  661 TTTCCTGACG CTCCATCTGC TCCTTGTGTG TCATAAACCA CACGTTTTTC CCGAAAATCT
  721 ATTCCCACTG TGGTGATGAA TTTGGGANTG AATTTATTGT CTGTGTATCT GTAGAGAAAT
  781 GTTGTCTTCC CGACTCCTGA NTCTCCAANG GCCANGANTT TAATCANATA ATCATAGTCT
  841 CCATCAGTCA TGGNTCTGAN TCCNGANTTG NACAGCTCGT CNNTGCCNAN AGTGATCCCN
  901 GCGGCGGTCA CGAACTCCAG CNNNNCATGT GATCNCNCTT CNCGTTGGGN NCTTTGCTCN
  961 NGNCGGANTG GGNGCTCNNN ANTGGNTGNN GGGNANCANC ANGGGGNCNN NNNNNANNGG
1021 GGNNNNCTNN NNGNANNNNN NNNNN
//
Primer: CMV_Forward (Pr0016)
Region: CMV pro, EGFP 5'
Sequence file: RDB17274_A9Elc.seq check
>D06280G2_A9El_2_CMV_Forward_C06_09_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNG CNNTGGGCGG TANGCGTGTA CGGTGGGNNG TCTATATAAG
   61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGCTAC CGGTCGCCAC CATGGTGAGC
  121 AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA
  181 AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG
  241 ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC
  301 ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA GCAGCACGAC
  361 TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC
  421 GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC
  481 ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG
  541 TACAACTACA ACAGCCACAA CGTCTATATC ATGGCCGACA AGCAGAAGAA CGGCATCAAG
  601 GTGAACTTCA AGATCCGCCA CAACATCGAG GACGGCAGCG TGCAGCTCGC CGACCACTAC
  661 CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC
  721 ACCCAGTCCG CCCTGANCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGNG
  781 TTCGTGACCG CCGCCGGGAT CACTCTCGGC ATGNNCGANC TNTACAAGTC CGGANTCAGA
  841 TCCATGNNTG ATGGANANTA TGANTATCNG ATTAAANTCC NGNCCNNNNN ANNNTCNNNA
  901 NTNNGGAAGA NANCATTNNT CTACANNNNN NNNGNNNNTA AANTNNNTCN NNANNNNNNN
961 NNN
//
Primer: SV40pro_F_V2 (Pr0154)
Region: NeoR_KanR
Sequence file: RDB17274_A9Eld.seq check
>D06280G2_A9El_2_SV40pro_F_V2_D06_12_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNA NGCTTTTGCN NAGNNCGATC AAGAGACAGG NTGAGGNNCG
   61 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG
  121 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG
  181 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT
  241 GAACTGCAAG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA
  301 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG
  361 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT
  421 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA
  481 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG
  541 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGAGCATG
  601 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG
  661 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGANCGCTAT
  721 CANGACATAG CGTTGGCTAC NCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC
  781 CGCTTCCTCG TGCTTTACNG TATCGCCGCT CCCNATTCGC AGCGCATCGC CTTCTATCGC
  841 CTTCTTGACG AGTNCTTCTG ANCGGGANTC TGGGGTTCGA AATGACCGAN NNAGCGACGC
901 CNNNCCNNNC NTCACNANAT TTCGANTNNN NNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Matsui, T., Small GTPase Rab12 regulates constitutive degradation of transferrin receptor. Traffic 12 (10): 1432-1443 (2011). PMID 21718402. [link to RRC of NBRP]
reference Fukuda, M., Slac2-a/melanophilin, the missing link between Rab27 and myosin Va: implications of a tripartite protein complex for melanosome transport. J. Biol. Chem., 277, 12432-12436 (2002). PMID 11856727. [link to RRC of NBRP]