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pEGFP-C1-Rab27B

Expression vector of mouse Rab27B fused with EGFP.

Catalog number RDB17274
Resource name pEGFP-C1-Rab27B
Clone info. Expression vector of mouse Rab27B fused with EGFP at N-terminus.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cell).
Gene/insert name mouse Rab27b cDNA
Reference of insert sequence AB232623 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
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External Database
mouse Rab27b

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Fukuda, M. et al. (2002) J. Biol. Chem. 277, 12432-12436); (Matsui, T. et al. (2011) Traffic 12, 1432-1443)
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Fukuda, M. et al. (2002) J. Biol. Chem. 277, 12432-12436); (Matsui, T. et al. (2011) Traffic 12, 1432-1443)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

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          RDB17274 pEGFP-C1-Rab27B DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Matsui, T., Small GTPase Rab12 regulates constitutive degradation of transferrin receptor. Traffic 12 (10): 1432-1443 (2011). PMID 21718402.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB17274_A9Elp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EGFP-C2
          Region: EGFP 3', insert 5'
          Sequence file: RDB17274_A9Ela.seq check
          >D06280G2_A9El_2_EGFP-C2_A06_03_ABI24.ab1
              1 NNNNNNNNNN NNNNNNCGNN CNCNTGGTCC TGCTGGNNTT CGTGNNCGCC GCCGGGATCA
             61 CTCTCGGCAT GGACGAGCTG TACAAGTCCG GACTCAGATC CATGACTGAT GGAGACTATG
            121 ATTATCTGAT TAAACTCCTG GCCCTTGGAG ACTCAGGAGT CGGGAAGACA ACATTTCTCT
            181 ACAGATACAC AGACAATAAA TTCAATCCCA AATTCATCAC CACAGTGGGA ATAGATTTTC
            241 GGGAAAAACG TGTGGTTTAT GACACACAAG GAGCAGATGG AGCGTCAGGA AAAGCGTTTA
            301 AGGTACATCT GCAGCTTTGG GACACTGCTG GACAAGAGCG GTTCCGGAGC CTTACCACTG
            361 CCTTCTTCAG AGATGCCATG GGCTTCTTAC TGATGTTTGA CCTCACCAGT CAACAGAGCT
            421 TCTTGAATGT CAGAAACTGG ATGAGTCAAC TGCAGGCAAA TGCTTACTGT GAAAACCCAG
            481 ACATAGTATT AATTGGCAAC AAGGCAGACC TGCCAGACCA AAGGGAAGTC AATGAACGGC
            541 AAGCACGGGA GCTGGCTGAA AAATATGGCA TACCATACTT CGAAACAAGC GCAGCTACAG
            601 GACAGAATGT AGAGAAGTCA GTGGAAACGC TTCTGGACTT AATCATGAAG AGAATGGAAA
            661 AGTGTGTAGA GAAGACACAG GTTCCGGACA CTGTCAATGG ANGAAATTCT GGANAGCTGG
            721 ATGGGGANNA GCCAGCAGAA AAGAAATGTG CCTGCTAGAA TCACTAGTGA ATTCTGCAGT
            781 CGANGGTACC GCGGGCCCGG GNNCCACCGG NTCTAGATAA CNGATCATAA TCAGCCNTAC
            841 CACATTTGTA GANGTTTTAC TNGCTTTNAA AANNNNNNNN NCNNNTCCNN NNNNNANNNN
          901 NNAANNTNAA ATGNANNGNN NNNNNNNN
          //
          Primer: SV40polyA-R2
          Region: insert 3'
          Sequence file: RDB17274_A9Elb.seq check
          >D06280G2_A9El_2_SV40polyA-R2_F03_18_ABI24.ab1
              1 NNNNNNNNNN NNNNNNAANN NGTTAACNNC NACNATTGCA TTCATTTTAT GTTTCAGGTT
             61 CAGGGGGAGG TGTGGGAGGT TTTTTAAAGC AAGTAAAACC TCTACAAATG TGGTATGGCT
            121 GATTATGATC AGTTATCTAG ATCCGGTGGA TCCCGGGCCC GCGGTACCGT CGACTGCAGA
            181 ATTCACTAGT GATTCTAGCA GGCACATTTC TTTTCTGCTG GCTTTTCCCC ATCCAGCTTT
            241 CCAGAATTTC CTCCATTGAC AGTGTCCGGA ACCTGTGTCT TCTCTACACA CTTTTCCATT
            301 CTCTTCATGA TTAAGTCCAG AAGCGTTTCC ACTGACTTCT CTACATTCTG TCCTGTAGCT
            361 GCGCTTGTTT CGAAGTATGG TATGCCATAT TTTTCAGCCA GCTCCCGTGC TTGCCGTTCA
            421 TTGACTTCCC TTTGGTCTGG CAGGTCTGCC TTGTTGCCAA TTAATACTAT GTCTGGGTTT
            481 TCACAGTAAG CATTTGCCTG CAGTTGACTC ATCCAGTTTC TGACATTCAA GAAGCTCTGT
            541 TGACTGGTGA GGTCAAACAT CAGTAAGAAG CCCATGGCAT CTCTGAAGAA GGCAGTGGTA
            601 AGGCTCCGGA ACCGCTCTTG TCCAGCAGTG TCCCAAAGCT GCAGATGTAC CTTAAACGCT
            661 TTTCCTGACG CTCCATCTGC TCCTTGTGTG TCATAAACCA CACGTTTTTC CCGAAAATCT
            721 ATTCCCACTG TGGTGATGAA TTTGGGANTG AATTTATTGT CTGTGTATCT GTAGAGAAAT
            781 GTTGTCTTCC CGACTCCTGA NTCTCCAANG GCCANGANTT TAATCANATA ATCATAGTCT
            841 CCATCAGTCA TGGNTCTGAN TCCNGANTTG NACAGCTCGT CNNTGCCNAN AGTGATCCCN
            901 GCGGCGGTCA CGAACTCCAG CNNNNCATGT GATCNCNCTT CNCGTTGGGN NCTTTGCTCN
            961 NGNCGGANTG GGNGCTCNNN ANTGGNTGNN GGGNANCANC ANGGGGNCNN NNNNNANNGG
          1021 GGNNNNCTNN NNGNANNNNN NNNNN
          //
          Primer: CMV_Forward
          Region: CMV pro, EGFP 5'
          Sequence file: RDB17274_A9Elc.seq check
          >D06280G2_A9El_2_CMV_Forward_C06_09_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNG CNNTGGGCGG TANGCGTGTA CGGTGGGNNG TCTATATAAG
             61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGCTAC CGGTCGCCAC CATGGTGAGC
            121 AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA
            181 AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG
            241 ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC
            301 ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA GCAGCACGAC
            361 TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC
            421 GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC
            481 ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG
            541 TACAACTACA ACAGCCACAA CGTCTATATC ATGGCCGACA AGCAGAAGAA CGGCATCAAG
            601 GTGAACTTCA AGATCCGCCA CAACATCGAG GACGGCAGCG TGCAGCTCGC CGACCACTAC
            661 CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC
            721 ACCCAGTCCG CCCTGANCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGNG
            781 TTCGTGACCG CCGCCGGGAT CACTCTCGGC ATGNNCGANC TNTACAAGTC CGGANTCAGA
            841 TCCATGNNTG ATGGANANTA TGANTATCNG ATTAAANTCC NGNCCNNNNN ANNNTCNNNA
            901 NTNNGGAAGA NANCATTNNT CTACANNNNN NNNGNNNNTA AANTNNNTCN NNANNNNNNN
          961 NNN
          //
          Primer: SV40pro_F_V2
          Region: NeoR_KanR
          Sequence file: RDB17274_A9Eld.seq check
          >D06280G2_A9El_2_SV40pro_F_V2_D06_12_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNA NGCTTTTGCN NAGNNCGATC AAGAGACAGG NTGAGGNNCG
             61 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG
            121 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG
            181 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT
            241 GAACTGCAAG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA
            301 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG
            361 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT
            421 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA
            481 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG
            541 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGAGCATG
            601 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG
            661 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGANCGCTAT
            721 CANGACATAG CGTTGGCTAC NCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC
            781 CGCTTCCTCG TGCTTTACNG TATCGCCGCT CCCNATTCGC AGCGCATCGC CTTCTATCGC
            841 CTTCTTGACG AGTNCTTCTG ANCGGGANTC TGGGGTTCGA AATGACCGAN NNAGCGACGC
          901 CNNNCCNNNC NTCACNANAT TTCGANTNNN NNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Matsui, T., Small GTPase Rab12 regulates constitutive degradation of transferrin receptor. Traffic 12 (10): 1432-1443 (2011). PMID 21718402.
          reference Fukuda, M., Slac2-a/melanophilin, the missing link between Rab27 and myosin Va: implications of a tripartite protein complex for melanosome transport. J. Biol. Chem., 277, 12432-12436 (2002). PMID 11856727.

          2022.07.13

          GNP_filter3_RDBDEP_html_220713.pl