Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEGFP-C1-Rab13 (#RDB17260)

Expression vector of mouse Rab13 fused with EGFP.

Clone info. Expression vector of mouse Rab13 fused with EGFP at N-terminus.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cell).
Gene/insert name mouse Rab13 cDNA
Reference of insert sequence AB232608 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Fukuda, M. (2003) J. Biol. Chem. 278, 15373-15380); (Tsuboi, T. and Fukuda, M. (2006) J. Cell Sci. 119, 2196-2203)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Fukuda, M. (2003) J. Biol. Chem. 278, 15373-15380); (Tsuboi, T. and Fukuda, M. (2006) J. Cell Sci. 119, 2196-2203)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17260 pEGFP-C1-Rab13 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-Rab13 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17260).

Reference section:

Tsuboi, T., Fukuda, M., Rab3A and Rab27A cooperatively regulate the docking step of dense-core vesicle exocytosis in PC12 cells. J. Cell Sci., 119 (Pt 11): 2196-2203 (2006). PMID 16684812. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17260_A9Elp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 (Pr0402)
Region: EGFP 3',insert 5'
Sequence file: RDB17260_A9Ela.seq check
>D06280E6_A9El_2_EGFP-C2_A04_01_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN GNNNNCNTGG TCCTGCTGGA GTTCGTGNCC GCCGCCGGGA
   61 TCACTCTCGG CATGGACGAG CTGTACAAGT CCGGACTCAG ATCCATGGCC AAAGCCTACG
  121 ACCACCTCTT CAAGTTGCTG CTCATCGGGG ACTCGGGGGT GGGCAAGACT TGTCTGATCA
  181 TTCGCTTTGC AGAGGACAAC TTCAACAGCA CTTACATCTC TACCATCGGA ATTGATTTCA
  241 AGATCCGAAC CGTGGACATA GAGGGGAAGA GGATCAAACT GCAAGTGTGG GACACGGCTG
  301 GCCAAGAACG ATTCAAGACA ATAACTACCG CCTATTACCG TGGAGCCATG GGCATTATCC
  361 TCGTATATGA CATCACAGAT GAGAAATCCT TCGAGAATAT TCAGAACTGG ATGAAAAGCA
  421 TCAAAGAGAA TGCCTCTGCG GGAGTGGAGC GCCTCCTGCT GGGAAACAAG TGTGACATGG
  481 AGGCCAAGCG GCAGGTGCAG AGAGAGCAGG CGGAGAAGTT GGCTCGAGAG CACAGAATCC
  541 GATTTTTTGA GACGAGTGCC AAATCCAGTG TGAATGTGGA TGAGGCTTTC AGTTCCCTGG
  601 CCCGTGACAT CTTGCTCAAG ACAGGAGGCC GGAGATCGGG AACCAACAGT AAGCCCTCAA
  661 GCACTGGCCT GAAAACATCT GACAAGAAGA ACAACAAGTG CTTGTTANGC TGAAATCACT
  721 AGTGAATTCT GCNGTCGACG GTACCGCGGG CCCGGGATCC ACCGGATCTA GATAACTGAT
  781 CATAATCAGC CATACCACAT TTGTAGAGGT TTTACTTGCT TTAAAAAACC TCCCACACCT
  841 CNNCNNGANC CTGAANCATN AAATGAATGN AATTGTTGTT GTTNNCTTGT TTATTGCAGC
  901 TTATAATGGT TACNAATNAA GCNATAGCAT CNCNAATTTC NCAAANNAAN NCNTTTTTTT
  961 CNCNGNATTC NAGTNGNNGN NTTNNNNNAN NTCNTNNNNG NATNNNANCG CNNNANNNNN
1021 NNNNN
//
Primer: SV40polyA_R2 (Pr0623)
Region: insert 3'
Sequence file: RDB17260_A9Elb.seq check
>D06280E6_A9El_2_SV40polyA-R2_B04_04_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNTT NNNNNNNNNN ATTGCNTTCA TTTTATGTTT
   61 CNGGTTCAGG GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT AAAACCTCTA CAAATGTGGT
  121 ATGGCTGATT ATGATCAGTT ATCTAGATCC GGTGGATCCC GGGCCCGCGG TACCGTCGAC
  181 TGCAGAATTC ACTAGTGATT TCAGCCTAAC AAGCACTTGT TGTTCTTCTT GTCAGATGTT
  241 TTCAGGCCAG TGCTTGAGGG CTTACTGTTG GTTCCCGATC TCCGGCCTCC TGTCTTGAGC
  301 AAGATGTCAC GGGCCAGGGA ACTGAAAGCC TCATCCACAT TCACACTGGA TTTGGCACTC
  361 GTCTCAAAAA ATCGGATTCT GTGCTCTCGA GCCAACTTCT CCGCCTGCTC TCTCTGCACC
  421 TGCCGCTTGG CCTCCATGTC ACACTTGTTT CCCAGCAGGA GGCGCTCCAC TCCCGCAGAG
  481 GCATTCTCTT TGATGCTTTT CATCCAGTTC TGAATATTCT CGAAGGATTT CTCATCTGTG
  541 ATGTCATATA CGAGGATAAT GCCCATGGCT CCACGGTAAT AGGCGGTAGT TATTGTCTTG
  601 AATCGTTCTT GGCCAGCCGT GTCCCACACT TGCAGTTTGA TCCTCTTCCC CTCTATGTCC
  661 ACGGTTCGGA TCTTGAAATC AATTCCGATG GTAGAGATGT AAGTGCTGTT GAAGTTGTCC
  721 TCTGCAAAGC GAATGATCAG ACAAGTCTTG CCCACCCCCG AGTCCCCGAT GANCANCAAC
  781 TTGAAGANGT GGTCGTANGC TTTGGNNNNG GATCTGANTC CNGACTTGTA CAGCTCGNCC
  841 ATGCNNAGAN TGNTCCCNGC NGGNNGGTCA CGAACTCNAG CANGANCATG TGATCGCNCT
  901 TCTCGTTGGG GGTCTTTGCN CNNGNCNGAN TNGGGTGCTC NNNNANTGGN TTGTCNGGCA
  961 GCANCNNNNN GNCCNTCNCC NANNNGGGNG NTCNNCNNGN NANNNNNNNG CNANCNNNNN
1021 NNNNN
//
Primer: CMV_Forward (Pr0016)
Region: CMV pro,EGFP 5'
Sequence file: RDB17260_A9Elc.seq check
>D06280E6_A9El_2_CMV-Forward_C04_07_ABI24.ab1
    1 NNNNNNNNNN NNNCNNNNNT GNNNNNNNTG GGCGGTANGC GTGTACGGTG GGAGGTCTAT
   61 ATAAGCAGAG CTGGTTTAGT GAACCGTCAG ATCCGCTAGC GCTACCGGTC GCCACCATGG
  121 TGAGCAAGGG CGAGGAGCTG TTCACCGGGG TGGTGCCCAT CCTGGTCGAG CTGGACGGCG
  181 ACGTAAACGG CCACAAGTTC AGCGTGTCCG GCGAGGGCGA GGGCGATGCC ACCTACGGCA
  241 AGCTGACCCT GAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCCTGG CCCACCCTCG
  301 TGACCACCCT GACCTACGGC GTGCAGTGCT TCAGCCGCTA CCCCGACCAC ATGAAGCAGC
  361 ACGACTTCTT CAAGTCCGCC ATGCCCGAAG GCTACGTCCA GGAGCGCACC ATCTTCTTCA
  421 AGGACGACGG CAACTACAAG ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA
  481 ACCGCATCGA GCTGAAGGGC ATCGACTTCA AGGAGGACGG CAACATCCTG GGGCACAAGC
  541 TGGAGTACAA CTACAACAGC CACAACGTCT ATATCATGGC CGACAAGCAG AAGAACGGCA
  601 TCAAGGTGAA CTTCAAGATC CGCCACAACA TCGAGGACGG CAGCGTGCAG CTCGCCGACC
  661 ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC
  721 TGAGCACCCA GTCCGCCCTG AGCAAAGACC CCAACGAGAA GCGCGATCAC ATGGTCCTGC
  781 TGGANTTCGT GACCGCCGCC GGGNTCACTC TCGGCATGGA NNANCTGTAC AAGTNCNGAN
  841 TCANATNCAT GGNCNAAGCC TACGANCACC TCTTCAAGTN NCTGCTCATC NGGGANTCNG
  901 GGGGNNGGCN ANANTNGTCT GATNNTTCGC TTNNCAGANG NNANCTTNNN NNNCACTTAC
961 NTCTCTANNN NNNNNNN
//
Primer: SV40pro_F_V2 (Pr0154)
Region: NeoR_KanR
Sequence file: RDB17260_A9Eld.seq check
>D06280E6_A9El_2_SV40pro_F_V2_D04_10_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNGCTTTN NNNNNNNNCG ATCNAGAGAN AGGANGAGGN
   61 NCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG
  121 AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC
  181 CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG
  241 AATGAACTGC AAGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC
  301 GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG
  361 CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT
  421 GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG
  481 AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT
  541 CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGAGC
  601 ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG
  661 GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGANCGC
  721 TATCANGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT
  781 GANCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT
  841 CGCCTTCTTG ACGANTTCNT CTGNNCGGGA NNCTGGGGGT TCNAAATGAN CGANCAAGCG
  901 ACGCCNNANC NNGNCATCAC GANNNTTCNA TTNCACCNCC GNCNTTCTAT GAAANNNNTN
  961 GGGCTTCNGN AANNNTTTTT NNNGGNNNNN CNNGNNNGNN GANNCNTNCN NNNNNNGGGN
1021 NNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Tsuboi, T., Rab3A and Rab27A cooperatively regulate the docking step of dense-core vesicle exocytosis in PC12 cells. J. Cell Sci., 119 (Pt 11): 2196-2203 (2006). PMID 16684812. [link to RRC of NBRP]
reference Fukuda, M., Distinct Rab binding specificity of Rim1, Rim2, rabphilin, and Noc2. Identification of a critical determinant of Rab3A/Rab27A recognition by Rim2. J. Biol. Chem. 278 (17): 15373-15380 (2003). PMID 12578829. [link to RRC of NBRP]