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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEGFP-C1-Rab12 (#RDB17259)

Expression vector of mouse Rab12 fused with EGFP.

Clone info. Expression vector of mouse Rab12 fused with EGFP at N-terminus.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cell).
Gene/insert name mouse Rab12 cDNA
Reference of insert sequence AB232607 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Fukuda, M. (2003) J. Biol. Chem. 278, 15373-15380); (Tsuboi, T. and Fukuda, M. (2006) J. Cell Sci. 119, 2196-2203)
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Fukuda, M. (2003) J. Biol. Chem. 278, 15373-15380); (Tsuboi, T. and Fukuda, M. (2006) J. Cell Sci. 119, 2196-2203)。

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RDB17259 pEGFP-C1-Rab12 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-Rab12 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17259).

Reference section:

Tsuboi, T., Fukuda, M., Rab3A and Rab27A cooperatively regulate the docking step of dense-core vesicle exocytosis in PC12 cells. J. Cell Sci., 119 (Pt 11): 2196-2203 (2006). PMID 16684812. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17259_A9Elp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: VN_F1 (Pr0992)
Region: EGFP 3',insert 5'
Sequence file: RDB17259_A9Ela.seq check
>D06280E5_A9El_2_VN_F1_C01_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNGCN NCNNNGTCTA TATNNTGGCC GANNAGCAGA AGAACGGCAT
   61 CAAGGTGAAC NNNNNNNTCC GCCACAACAT CGAGGACGGC AGCGTGCAGC TCGCCGACCA
  121 CTACCAGCAG AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT
  181 GAGCACCCAG TCCGCCCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT
  241 GGAGTTCGTG ACCGCCGCCG GGATCACTCT CGGCATGGAC GAGCTGTACA AGTCCGGACT
  301 CAGATCTATG GATCCCAGCG CCGCGCTGCA CCGACGGCCC GCGGGCGGCA GCCTGGGCGC
  361 GGTCTCCCCG GCGCTGTCGG GCGGCCAGGC CCGCCGGAGG AAGCAGCCCC CCAGGCCGGC
  421 CGACTTCAAG CTGCAGGTCA TCATCATCGG CTCCCGCGGC GTGGGCAAGA CCAGCCTGAT
  481 GGAGCGCTTC ACCGACGACA CGTTCTGCGA GGCCTGCAAG TCCACCGTGG GTGTTGACTT
  541 TAAAATCAAG ACTGTAGAAC TAAGAGGGAA GAAAATTAGA TTACAGATCT GGGACACAGC
  601 AGGGCAGGAG CGATTTAACA GCATTACCTC AGCCTATTAC AGAAGTGCCA AGGGGATCAT
  661 ACTCGTATAT GACATCACCA AGAAGGAGAC GTTCGATGAC TTGCCNAAGT GGATGAAGAT
  721 GANTGACAAG TATGCTTCAG AAGACGCCGA GCTCCTCCTG GTTGGAAANN NNNNGGACTG
  781 TGAGACAGAC AGAGAAATCT CANGGCAGCA AGGANAAAAG TTNGCACAGC ANANAACCGG
  841 GATGCGCTTC TGCNAANCCN GTGCCAANGN NANTTTCNAN GTGGNNNANN TCTTTCNGAA
  901 ACTCGNNGAN NNNATTCTGA AAAANANGNN NNTNGNNNNN NNNGNANNNN NNNNNTCNNN
961 NANCNTNCNN TTCNNNNNNA NCNNNNNNNN NN
//
Primer: SV40polyA_R2 (Pr0623)
Region: insert 3'
Sequence file: RDB17259_A9Elb.seq check
>D06280E5_A9El_2_SV40polyA-R2_F03_18_ABI24.ab1
    1 NNNNNNNNNN NNNANNNNNA GTTAANNANA ACNATTGCAT TCNTTTTATG TTTCNGGTTC
   61 AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA AGTAAAACCT CTACAAATGT GGTATGGCTG
  121 ATTATGATCA GTTATCTAGA TCCGGTGGAT CCCGGGCCCG CGGTACCGTC GACTGCAGAA
  181 TTCACTAGTG ATTCTCCAAA GTATCAAATC AACAGCATCG GACGTGTGGT CTGGGAGGAG
  241 GCAGCTCTGG TGGGATCTCA GGCTCTGGTT GTAGAGAAAG GATGCTATTG GATAACTCGT
  301 TTCTCAAAAC ATCAAGAGGC ATCTTTTTCA GAATATCATC AACGAGTTTC AGAAAGATCT
  361 CGTCCACATT GAAATTGTCC TTGGCACTGG CTTCGCAGAA GCGCATCCCG GTTATCTGCT
  421 GTGCAAACTT TTCTCCTTGC TGCCTTGAGA TTTCTCTGTC TGTCTCACAG TCCAGCTTAT
  481 TTCCAACCAG GAGGAGCTCG GCGTCTTCTG AAGCATACTT GTCAATCATC TTCATCCACT
  541 TTGGCAAGTC ATCGAACGTC TCCTTCTTGG TGATGTCATA TACGAGTATG ATCCCCTTGG
  601 CACTTCTGTA ATAGGCTGAG GTAATGCTGT TAAATCGCTC CTGCCCTGCT GTGTCCCAGA
  661 TCTGTAATCT AATTTTCTTC CCTCTTAGTT CTACAGTCTT GATTTTAAAG TCAACACCCA
  721 CGGTGGACTT GCAGGCCTCG CAGAACGTGT CGTCNGTGAA GCGCTCCATC ANGCTGGTCT
  781 TGCCCACGCC GCGGGANCCG ATGANGANGA CCTGCAGCTT GAAGTCGGCC GGCCTGGGGG
  841 GCTGCTTNCT CCGGCGGGCC TGGCCGCCCG ANAGCGCCNG GGANANCGCG CCCNGNCTGN
  901 CGCCCGCGGG CCGTCGGTGC ANCNCNNCGC TNGGNTNCAT ANATCTGANT NNGGANNNGT
  961 ACAGCTCGTC CNTGCCGANA NTGNTNCNNG NNNNNGNNNN NNANNNNNAN CNNNANNNTG
1021 NGATNNNNNN NNNN
//
Primer: CMV_Forward (Pr0016)
Region: CMV pro,EGFP 5'
Sequence file: RDB17259_A9Elc.seq check
>D06280E5_A9El_2_CMV-Forward_G03_21_ABI24.ab1
    1 NNNNNNNNNN NNNNNNTTGN NGCNNNTGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT
   61 AAGCAGAGCT GGTTTAGTGA ACCGTCAGAT CCGCTAGCGC TACCGGTCGC CACCATGGTG
  121 AGCAAGGGCG AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC
  181 GTAAACGGCC ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC CTACGGCAAG
  241 CTGACCCTGA AGTTCATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG
  301 ACCACCCTGA CCTACGGCGT GCAGTGCTTC AGCCGCTACC CCGACCACAT GAAGCAGCAC
  361 GACTTCTTCA AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT CTTCTTCAAG
  421 GACGACGGCA ACTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC CCTGGTGAAC
  481 CGCATCGAGC TGAAGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG GCACAAGCTG
  541 GAGTACAACT ACAACAGCCA CAACGTCTAT ATCATGGCCG ACAAGCAGAA GAACGGCATC
  601 AAGGTGAACT TCAAGATCCG CCACAACATC GAGGACGGCA GCGTGCAGCT CGCCGACCAC
  661 TACCAGCAGA ACACCCCCAT CGGCGACGGC CCCGTGCTGC TGCCCGACAA CCACTACCTG
  721 AGCACCCAGT CCGCCCTGAG CAAAGANCCC AACGAGAAGC GCGATCACAT GGTCCTGCTG
  781 GAGTTCGTGA NCGCCGCCGG GATCACTCTC GGCATGGNNG ANCTGTACNA GTNCNGANTC
  841 AGATCTATGG ATNCCAGCGC CGCGCTGCAC CGACNGNCCG CGGGCGGNAG NCTGGGCGCG
  901 GNCNNCCCGG NGCTNNNGGG CNGNCAGGCC CNCCNGNANG AANCANCCCN NNNNNNCNNN
  961 NCGANTTNNN GCTNNNNNNC NTNNNCATNN NNTNCNNCNN NNNGGNNNNN ANNNNNCNNN
1021 NNNGNNNCNN NNNNNNNN
//
Primer: SV40pro_F_V2 (Pr0154)
Region: NeoR_KanR
Sequence file: RDB17259_A9Eld.seq check
>D06280E5_A9El_2_SV40pro_F_V2_H03_24_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNGCTTTNNN NNNNNNCGAT CNAGAGACAG GATGAGGANC
   61 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
  121 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
  181 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
  241 TGAACTGCAA GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
  301 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
  361 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
  421 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
  481 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
  541 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGAGCAT
  601 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
  661 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA
  721 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA
  781 CCGCTTCCTC GTGCTTTACN GTATCGCCGC TCCNGATTCG CAGCGCATCG CCTTCTATCG
  841 CCTTCTTGAC GAGTTCTTCT GAGCGGGANT CTNGGGTNCG AAATGACCGA NCAAGCGACG
  901 CCCAACCTGC CNTCACGAGA TTTCNATTCC NNCGCCGCCT TCTATGAAAN GNNGGGCTTC
  961 NGANNCNTTT TNCNGGGANG NNNGNTGGNN GATNCNTNNN NCNNGGGGNN NNCANGNNNG
1021 NNNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Tsuboi, T., Rab3A and Rab27A cooperatively regulate the docking step of dense-core vesicle exocytosis in PC12 cells. J. Cell Sci., 119 (Pt 11): 2196-2203 (2006). PMID 16684812. [link to RRC of NBRP]
reference Fukuda, M., Distinct Rab binding specificity of Rim1, Rim2, rabphilin, and Noc2. Identification of a critical determinant of Rab3A/Rab27A recognition by Rim2. J. Biol. Chem. 278 (17): 15373-15380 (2003). PMID 12578829. [link to RRC of NBRP]