Please review the QC test results indicated by icon below as well as clone information before placing your order.

pEGFP-C1-Rab5A

Expression vector of mouse Rab5A fused with EGFP.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kuroda, T. S. et al. (2002) J. Biol. Chem. 277, 9212-9218); (Tsuboi, T. and Fukuda, M. (2006) J. Cell Sci. 119, 2196-2203)
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kuroda, T. S. et al. (2002) J. Biol. Chem. 277, 9212-9218); (Tsuboi, T. and Fukuda, M. (2006) J. Cell Sci. 119, 2196-2203)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB17246_A9Elp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 (Pr0402) Region: EGFP, insert 5' Sequence file: RDB17246_A9Ela.seq
>D06280D1 _A9El_1_EGFP-C2_A06_03_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNC GNTNNNNNGG TCCTGCTGGA NTTCGTGANC GCCGCCGGGA    61 TCACTCTCGG CATGGACGAG CTGTACAAGT CCGGACTCAG ATCCATGGCT AATCGAGGAG   121 CAACAAGACC CAACGGGCCA AATACTGGAA ATAAAATATG CCAGTTCAAA CTGGTCCTTC   181 TAGGAGAGTC TGCTGTTGGC AAATCAAGCC TGGTTCTTCG CTTTGTGAAA GGCCAATTTC   241 ATGAATTTCA AGAGAGTACC ATTGGGGCTG CCTTTCTAAC CCAAACTGTG TGTCTTGATG   301 ACACAACAGT AAAATTTGAA ATATGGGATA CAGCTGGTCA AGAACGGTAT CATAGCTTAG   361 CACCAATGTA CTACCGAGGA GCACAAGCAG CCATAGTTGT GTATGATATC ACAAATGAGG   421 AATCCTTTGC GAGAGCAAAA AACTGGGTTA AAGAACTTCA AAGGCAAGCA AGTCCTAATA   481 TTGTGATAGC TTTGTCAGGA AACAAAGCTG ACTTAGCAAA TAAAAGAGCT GTTGACTTCC   541 AGGAAGCACA GTCCTATGCA GATGACAACA GCTTATTATT TATGGAGACA TCAGCTAAGA   601 CATCAATGAA TGTAAATGAA ATATTTATGG CAATAGCTAA AAAGCTGCCA AAGAATGAAC   661 CACAGAATCC TGGTGCAAAC TCAGCCAGAN GACGAGGAGT AGACCTTACT GAGCCTGCNC   721 AGCCAGCCAG AAGCCAGTGT TGTAGTAACT GAAATCGAAT TCTGCANTCN ANNGTACCGC   781 GGGCCCGGGA TCCANCGGAT CTNGATAACT GATCATNNTC AGCCNTACNA CATTTGTAGA   841 NGTTTTACTT GCNTNNAAAA ANCTCCCNCA NNNCCNCNNG AACCTGAAAC ATAAANNGAA   901 TGCAATTGTT GNTGTNAACN TGTTTNNNGN NNCNNANNAN NGGNNNNAAA NNAANNNNNN 961 CANNNNNAAN TNNNCNNNNA NNNNNTTTTT TTTCNCNGNN NNNNNNNN //
Primer: SV40polyA_R2 (Pr0623) Region: insert 3' Sequence file: RDB17246_A9Elb.seq
>D06280D1_A9El_1_SV40polyA-R2_B06_06_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNACNATTG CATTCNTTTT ATGTTTCAGG    61 TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGGTATGG   121 CTGATTATGA TCAGTTATCT AGATCCGGTG GATCCCGGGC CCGCGGTACC GTCGACTGCA   181 GAATTCGATT TCAGTTACTA CAACACTGGC TTCTGGCTGG CTGTGCAGGC TCAGTAAGGT   241 CTACTCCTCG TCCTCTGGCT GAGTTTGCAC CAGGATTCTG TGGTTCATTC TTTGGCAGCT   301 TTTTAGCTAT TGCCATAAAT ATTTCATTTA CATTCATTGA TGTCTTAGCT GATGTCTCCA   361 TAAATAATAA GCTGTTGTCA TCTGCATAGG ACTGTGCTTC CTGGAAGTCA ACAGCTCTTT   421 TATTTGCTAA GTCAGCTTTG TTTCCTGACA AAGCTATCAC AATATTAGGA CTTGCTTGCC   481 TTTGAAGTTC TTTAACCCAG TTTTTTGCTC TCGCAAAGGA TTCCTCATTT GTGATATCAT   541 ACACAACTAT GGCTGCTTGT GCTCCTCGGT AGTACATTGG TGCTAAGCTA TGATACCGTT   601 CTTGACCAGC TGTATCCCAT ATTTCAAATT TTACTGTTGT GTCATCAAGA CACACAGTTT   661 GGGTTAGAAA GGCAGCCCCA ATGGTACTCT CTTGAAATTC ATGAAATTGG CCTTTCACAA   721 AGCGAAGAAC CAGGCTTGAT TTGCCNACAG CAGACTCTCC TANAANGANC AGTTTGAACT   781 GGCATATTTT ATTTCCNGTA TTTGGCCCGT TGGGTCNTGT TGCTCCTCGA TTAGCCATGG   841 NTCTGANTCC GGANTTGTAC AGCTCGTNCN TGCCNANAGT GNTNCCGGCG GNNGNCANNA   901 ACTCCANCNN NNNNTGTGAT CGCNCNTCTC GTTGGGGNCT TTGCTCNNGN CGGNNTGGGN 961 GNTCNNNANN GGTTGTCNGG CNNCNNCNCG GGGNNNTCNN NNNNGGGGGN NNNNNNNNN //
Primer: CMV_Forward (Pr0016) Region: CMV pro, EGFP 5' Sequence file: RDB17246_A9Elc.seq
>D06280D1_A9El_1_CMV-Forward_C06_09_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNTGNNGNNN NNGGCGGTAG GCGTGTACGG TGGGAGGTCT    61 ATATAAGCAG AGCTGGTTTA GTGAACCGTC AGATCCGCTA GCGCTACCGG TCGCCACCAT   121 GGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG   181 CGACGTAAAC GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG   241 CAAGCTGACC CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT   301 CGTGACCACC CTGACCTACG GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA   361 GCACGACTTC TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT   421 CAAGGACGAC GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT   481 GAACCGCATC GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA   541 GCTGGAGTAC AACTACAACA GCCACAACGT CTATATCATG GCCGACAAGC AGAAGAACGG   601 CATCAAGGTG AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA   661 CCACTACCAG CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA   721 CCTGAGCACC CAGTCCGCCC TGAGCAAAGA CCCCAACGAN AAGCGCGATC ACATGGTCCT   781 GCTGGAGTTC GTGANCGCCG CCGGGATCAC TCTCGGCATG GANGANCTGT ACAAGTCCGG   841 ACTCAGATCC ATGGCTNATC GAGGANNNAC NNNANCCNAN GGGCCNAATA CTGNAAATAA   901 AATATGCCAN TNNAAACTGG TCCTNNNNNN ANAGTCTGCT GTTGNCAAAT NNNGCNNNGN   961 TTNNNCNNTT TGTNANNNCN NANTTNNNTG ANTTTCNNGA GAGTANNNTT GGGNNNNNNN 1021 NNN //
Primer: SV40pro_F_V2 (Pr0154) Region: NeoR_KanR Sequence file: RDB17246_A9Eld.seq
>D06280D1_A9El_1_SV40pro_F_V2_D06_12_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNGCTTT TGCNNAGNNC GATCNAGAGA CNGGATGAGG    61 NNCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA   121 GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT   181 CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT   241 GAATGAACTG CAAGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG   301 CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT   361 GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC   421 TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC   481 GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA   541 TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGAG   601 CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT   661 GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGANCG   721 CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC   781 TGACCGCTTC CTCGTGCTTT ACNGTATCGC CGCTCCCNAT TCNCAGCGCA TCGCCTTCTA   841 TCGCCTTCTT GACGAGTTCN TCTGANCGGG ANTCTGGGGN NNGAAATGAC CGANCNNGCG   901 ACGCCCNACC TGNNNTCNCN AGATTNNNAT TCCNCCNCCG CCNTCTATNA ANNGNTGGGG   961 CTTCNGNANN NNTTTTNNNG NNNNNCNNGN NNGNNNGANN NNNNNNCNNN GGGNNNNNCN 1021 NNNNNGNNNN NNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.04.06

GNP_filter3_RDBDEP_html_230405.pl