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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEGFP-C1-Rab5A (#RDB17246)

Expression vector of mouse Rab5A fused with EGFP.

Clone info. Expression vector of mouse Rab5A fused with EGFP at N-terminus.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cell).
Gene/insert name mouse Rab5a cDNA
Reference of insert sequence AB232593 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kuroda, T. S. et al. (2002) J. Biol. Chem. 277, 9212-9218); (Tsuboi, T. and Fukuda, M. (2006) J. Cell Sci. 119, 2196-2203)
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kuroda, T. S. et al. (2002) J. Biol. Chem. 277, 9212-9218); (Tsuboi, T. and Fukuda, M. (2006) J. Cell Sci. 119, 2196-2203)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17246 pEGFP-C1-Rab5A DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-Rab5A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17246).

Reference section:

Tsuboi, T., Fukuda, M., Rab3A and Rab27A cooperatively regulate the docking step of dense-core vesicle exocytosis in PC12 cells. J. Cell Sci., 119 (Pt 11): 2196-2203 (2006). PMID 16684812. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17246_A9Elp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 (Pr0402)
Region: EGFP, insert 5'
Sequence file: RDB17246_A9Ela.seq check
>D06280D1 _A9El_1_EGFP-C2_A06_03_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNC GNTNNNNNGG TCCTGCTGGA NTTCGTGANC GCCGCCGGGA
   61 TCACTCTCGG CATGGACGAG CTGTACAAGT CCGGACTCAG ATCCATGGCT AATCGAGGAG
  121 CAACAAGACC CAACGGGCCA AATACTGGAA ATAAAATATG CCAGTTCAAA CTGGTCCTTC
  181 TAGGAGAGTC TGCTGTTGGC AAATCAAGCC TGGTTCTTCG CTTTGTGAAA GGCCAATTTC
  241 ATGAATTTCA AGAGAGTACC ATTGGGGCTG CCTTTCTAAC CCAAACTGTG TGTCTTGATG
  301 ACACAACAGT AAAATTTGAA ATATGGGATA CAGCTGGTCA AGAACGGTAT CATAGCTTAG
  361 CACCAATGTA CTACCGAGGA GCACAAGCAG CCATAGTTGT GTATGATATC ACAAATGAGG
  421 AATCCTTTGC GAGAGCAAAA AACTGGGTTA AAGAACTTCA AAGGCAAGCA AGTCCTAATA
  481 TTGTGATAGC TTTGTCAGGA AACAAAGCTG ACTTAGCAAA TAAAAGAGCT GTTGACTTCC
  541 AGGAAGCACA GTCCTATGCA GATGACAACA GCTTATTATT TATGGAGACA TCAGCTAAGA
  601 CATCAATGAA TGTAAATGAA ATATTTATGG CAATAGCTAA AAAGCTGCCA AAGAATGAAC
  661 CACAGAATCC TGGTGCAAAC TCAGCCAGAN GACGAGGAGT AGACCTTACT GAGCCTGCNC
  721 AGCCAGCCAG AAGCCAGTGT TGTAGTAACT GAAATCGAAT TCTGCANTCN ANNGTACCGC
  781 GGGCCCGGGA TCCANCGGAT CTNGATAACT GATCATNNTC AGCCNTACNA CATTTGTAGA
  841 NGTTTTACTT GCNTNNAAAA ANCTCCCNCA NNNCCNCNNG AACCTGAAAC ATAAANNGAA
  901 TGCAATTGTT GNTGTNAACN TGTTTNNNGN NNCNNANNAN NGGNNNNAAA NNAANNNNNN
961 CANNNNNAAN TNNNCNNNNA NNNNNTTTTT TTTCNCNGNN NNNNNNNN
//
Primer: SV40polyA_R2 (Pr0623)
Region: insert 3'
Sequence file: RDB17246_A9Elb.seq check
>D06280D1_A9El_1_SV40polyA-R2_B06_06_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNACNATTG CATTCNTTTT ATGTTTCAGG
   61 TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGGTATGG
  121 CTGATTATGA TCAGTTATCT AGATCCGGTG GATCCCGGGC CCGCGGTACC GTCGACTGCA
  181 GAATTCGATT TCAGTTACTA CAACACTGGC TTCTGGCTGG CTGTGCAGGC TCAGTAAGGT
  241 CTACTCCTCG TCCTCTGGCT GAGTTTGCAC CAGGATTCTG TGGTTCATTC TTTGGCAGCT
  301 TTTTAGCTAT TGCCATAAAT ATTTCATTTA CATTCATTGA TGTCTTAGCT GATGTCTCCA
  361 TAAATAATAA GCTGTTGTCA TCTGCATAGG ACTGTGCTTC CTGGAAGTCA ACAGCTCTTT
  421 TATTTGCTAA GTCAGCTTTG TTTCCTGACA AAGCTATCAC AATATTAGGA CTTGCTTGCC
  481 TTTGAAGTTC TTTAACCCAG TTTTTTGCTC TCGCAAAGGA TTCCTCATTT GTGATATCAT
  541 ACACAACTAT GGCTGCTTGT GCTCCTCGGT AGTACATTGG TGCTAAGCTA TGATACCGTT
  601 CTTGACCAGC TGTATCCCAT ATTTCAAATT TTACTGTTGT GTCATCAAGA CACACAGTTT
  661 GGGTTAGAAA GGCAGCCCCA ATGGTACTCT CTTGAAATTC ATGAAATTGG CCTTTCACAA
  721 AGCGAAGAAC CAGGCTTGAT TTGCCNACAG CAGACTCTCC TANAANGANC AGTTTGAACT
  781 GGCATATTTT ATTTCCNGTA TTTGGCCCGT TGGGTCNTGT TGCTCCTCGA TTAGCCATGG
  841 NTCTGANTCC GGANTTGTAC AGCTCGTNCN TGCCNANAGT GNTNCCGGCG GNNGNCANNA
  901 ACTCCANCNN NNNNTGTGAT CGCNCNTCTC GTTGGGGNCT TTGCTCNNGN CGGNNTGGGN
961 GNTCNNNANN GGTTGTCNGG CNNCNNCNCG GGGNNNTCNN NNNNGGGGGN NNNNNNNNN
//
Primer: CMV_Forward (Pr0016)
Region: CMV pro, EGFP 5'
Sequence file: RDB17246_A9Elc.seq check
>D06280D1_A9El_1_CMV-Forward_C06_09_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNTGNNGNNN NNGGCGGTAG GCGTGTACGG TGGGAGGTCT
   61 ATATAAGCAG AGCTGGTTTA GTGAACCGTC AGATCCGCTA GCGCTACCGG TCGCCACCAT
  121 GGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG
  181 CGACGTAAAC GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG
  241 CAAGCTGACC CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT
  301 CGTGACCACC CTGACCTACG GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA
  361 GCACGACTTC TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT
  421 CAAGGACGAC GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT
  481 GAACCGCATC GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA
  541 GCTGGAGTAC AACTACAACA GCCACAACGT CTATATCATG GCCGACAAGC AGAAGAACGG
  601 CATCAAGGTG AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA
  661 CCACTACCAG CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA
  721 CCTGAGCACC CAGTCCGCCC TGAGCAAAGA CCCCAACGAN AAGCGCGATC ACATGGTCCT
  781 GCTGGAGTTC GTGANCGCCG CCGGGATCAC TCTCGGCATG GANGANCTGT ACAAGTCCGG
  841 ACTCAGATCC ATGGCTNATC GAGGANNNAC NNNANCCNAN GGGCCNAATA CTGNAAATAA
  901 AATATGCCAN TNNAAACTGG TCCTNNNNNN ANAGTCTGCT GTTGNCAAAT NNNGCNNNGN
  961 TTNNNCNNTT TGTNANNNCN NANTTNNNTG ANTTTCNNGA GAGTANNNTT GGGNNNNNNN
1021 NNN
//
Primer: SV40pro_F_V2 (Pr0154)
Region: NeoR_KanR
Sequence file: RDB17246_A9Eld.seq check
>D06280D1_A9El_1_SV40pro_F_V2_D06_12_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNGCTTT TGCNNAGNNC GATCNAGAGA CNGGATGAGG
   61 NNCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA
  121 GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT
  181 CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT
  241 GAATGAACTG CAAGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG
  301 CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT
  361 GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC
  421 TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC
  481 GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA
  541 TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGAG
  601 CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT
  661 GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGANCG
  721 CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC
  781 TGACCGCTTC CTCGTGCTTT ACNGTATCGC CGCTCCCNAT TCNCAGCGCA TCGCCTTCTA
  841 TCGCCTTCTT GACGAGTTCN TCTGANCGGG ANTCTGGGGN NNGAAATGAC CGANCNNGCG
  901 ACGCCCNACC TGNNNTCNCN AGATTNNNAT TCCNCCNCCG CCNTCTATNA ANNGNTGGGG
  961 CTTCNGNANN NNTTTTNNNG NNNNNCNNGN NNGNNNGANN NNNNNNCNNN GGGNNNNNCN
1021 NNNNNGNNNN NNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Tsuboi, T., Rab3A and Rab27A cooperatively regulate the docking step of dense-core vesicle exocytosis in PC12 cells. J. Cell Sci., 119 (Pt 11): 2196-2203 (2006). PMID 16684812. [link to RRC of NBRP]
reference Kuroda, T.S., The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain. J. Biol. Chem., 277, 9212-9218 (2002). PMID 11773082. [link to RRC of NBRP]