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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEGFP-C1-Rab3B (#RDB17241)

Expression vector of mouse Rab3B fused with EGFP.

Clone info. Expression vector of mouse Rab3B fused with EGFP at N-terminus.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cell).
Gene/insert name mouse Rab3b cDNA
Reference of insert sequence AB232588 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

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Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Fukuda, M. (2003) J. Biol. Chem. 278, 15373-15380); (Matsui, T. et al. (2011) Traffic 12, 1432-1443)
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Fukuda, M. (2003) J. Biol. Chem. 278, 15373-15380); (Matsui, T. et al. (2011) Traffic 12, 1432-1443)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17241 pEGFP-C1-Rab3B DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-Rab3B was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17241).

Reference section:

Matsui, T., Itoh, T., Fukuda, M., Small GTPase Rab12 regulates constitutive degradation of transferrin receptor. Traffic 12 (10): 1432-1443 (2011). PMID 21718402. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17241_A9Elp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 (Pr0402)
Region: EGFP 3', insert
Sequence file: RDB17241_A9Ela.seq check
>D06280C5_A9El_2_EGFP-C2_E03_15_ABI24.ab1
    1 NNNNNNNNNN NNNNNNGNNN NCNTGGTCCT GCTGGAGTTC GTGANCGCCG CCGGGNTCAC
   61 TCTCGGCATG GACGAGCTGT ACAAGTCCGG ACTCAGATCC ATGGCTTCAG TGACTGATGG
  121 TAAGACTGGA ATCAAAGATG CCTCCGACCA GAACTTTGAC TACATGTTCA AACTGCTCAT
  181 CATTGGCAAC AGCAGCGTCG GGAAGACATC CTTCCTTTTC CGCTATGCTG ATGACACCTT
  241 CACCCCTGCC TTTGTCAGCA CTGTGGGTAT CGACTTCAAA GTGAAGACAG TCTACCGCCA
  301 TGAGAAGCGT GTGAAGCTGC AGATATGGGA CACAGCTGGG CAAGAGCGGT ACCGGACCAT
  361 CACCACAGCC TACTACCGTG GGGCCATGGG CTTCATTCTC ATGTATGACA TCACCAACGA
  421 GGAGTCCTTC AATGCTGTCC AGGACTGGGC TACTCAGATT AAGACCTACT CCTGGGACAA
  481 CGCACAGGTC ATTCTCGTGG GAAATAAGTG TGACATGGAA GAGGAAAGAG TGGTCCCAAC
  541 TGAGAAGGGC CGGCTCCTGG CAGAACAGCT TGGGTTTGAC TTCTTTGAAG CCAGCGCCAA
  601 GGAGAACATC AGTGTGAGGC AGGCCTTCGA GCGGCTGGTG GATGCCATCT GCGATAAGAT
  661 GTCTGACTCG ATGGACACAG ACCCCTCCGT GCTAGGCGCC TCCAAGACGA CGCGGCTCTC
  721 AGACACCCCA CCTCTGCTGC AGCAGAACTG CTCTTGCTAG AATCGAATTC TGCAGTCGAC
  781 GGTACCGCGG GCCCGGGATC CACCGGATCT AGATAACTGA TCATAATCAG CCATACCACA
  841 TTTGTAGANG NTTTACTTGC TTTAAAAAAC CTCCCACACC NCCCCCNGAA CCTGAAACNT
  901 AAAATGAATG NNATTGNTGT TGTTAACTTG TTTATTGCAG CTTATAATGG NTACAAATAA
961 ANCNANANCN TCNNAANNTT CNNNAATAAN NNNNTTTTTN NNNNNNNNTN NNNNNNNNN
//
Primer: SV40polyA_R2 (Pr0623)
Region: insert 3'
Sequence file: RDB17241_A9Elb.seq check
>D06280C5_A9El_2_SV40polyA-R2_F03_18_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNTNNAN NNNNACNATT GCATTCNTTT TATGTTTCNG
   61 GTTCAGGGGG AGGTGTGGGA GGTTTTTTAA AGCAAGTAAA ACCTCTACAA ATGTGGTATG
  121 GCTGATTATG ATCAGTTATC TAGATCCGGT GGATCCCGGG CCCGCGGTAC CGTCGACTGC
  181 AGAATTCGAT TCTAGCAAGA GCAGTTCTGC TGCAGCAGAG GTGGGGTGTC TGAGAGCCGC
  241 GTCGTCTTGG AGGCGCCTAG CACGGAGGGG TCTGTGTCCA TCGAGTCAGA CATCTTATCG
  301 CAGATGGCAT CCACCAGCCG CTCGAAGGCC TGCCTCACAC TGATGTTCTC CTTGGCGCTG
  361 GCTTCAAAGA AGTCAAACCC AAGCTGTTCT GCCAGGAGCC GGCCCTTCTC AGTTGGGACC
  421 ACTCTTTCCT CTTCCATGTC ACACTTATTT CCCACGAGAA TGACCTGTGC GTTGTCCCAG
  481 GAGTAGGTCT TAATCTGAGT AGCCCAGTCC TGGACAGCAT TGAAGGACTC CTCGTTGGTG
  541 ATGTCATACA TGAGAATGAA GCCCATGGCC CCACGGTAGT AGGCTGTGGT GATGGTCCGG
  601 TACCGCTCTT GCCCAGCTGT GTCCCATATC TGCAGCTTCA CACGCTTCTC ATGGCGGTAG
  661 ACTGTCTTCA CTTTGAAGTC GATACCCACA GTGCTGACAA AGGCAGGGGT GAAGGTGTCA
  721 TCAGCATAGC GGAAAAGGAA GGATGTCTTC CCGACGCTGC TGTTGCCAAT GATGAGCAGT
  781 TTGAACATGT AGTCAAAGTT CTGGTCGGAN GCATCTTTGA TTCCAGTCTT ACCATCAGTC
  841 ACTGAAGCCA TGGATCTGAN TCCGGANTTG TACAGCTCGT CCNTGCCGAG AGTGATCCCG
  901 GCGGNNGNCA CNAACNCCAG CNNNANCNNN TGATCGCNCT TCTCNTTGGG GNCTTNGCNC
  961 NNGNNGNNNN NGGNGNTCNN NANTGNNTNN NNGGGCANNA NCNNGGGGNC NNNNNNGATG
1021 GGGGNNNNNN NNNN
//
Primer: CMV_Forward (Pr0016)
Region: CMV pro, EGFP 5'
Sequence file: RDB17241_A9Elc.seq check
>D06280C5_A9El_2_CMV-Forward_G03_21_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNTTG NNNNNNATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
   61 TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCCGCTAGCG CTACCGGTCG CCACCATGGT
  121 GAGCAAGGGC GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA
  181 CGTAAACGGC CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA
  241 GCTGACCCTG AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT
  301 GACCACCCTG ACCTACGGCG TGCAGTGCTT CAGCCGCTAC CCCGACCACA TGAAGCAGCA
  361 CGACTTCTTC AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA
  421 GGACGACGGC AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA
  481 CCGCATCGAG CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT
  541 GGAGTACAAC TACAACAGCC ACAACGTCTA TATCATGGCC GACAAGCAGA AGAACGGCAT
  601 CAAGGTGAAC TTCAAGATCC GCCACAACAT CGAGGACGGC AGCGTGCAGC TCGCCGACCA
  661 CTACCAGCAG AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT
  721 GANCACCCAG TCCGCCCTGA GCAAAGANCC CAACGANAAG CGCGATCACA TGGTCCTGCT
  781 GGANTTCGTG ACCGCCGCCG GGATCACTCT CGGCATGNNC GANCTGTACA AGTCCGGANT
  841 CANATNCATG GCTTCAGTGN NNGATGGTAA NANTGGAATC AAAGATGCCT CCGANCAGAA
  901 CTTTGANNNC NTGTTCNAAC TGCTCATCAT NNNCNACAGC ANCGTCGGGN NNNNNTCCTT
961 NCNTTTTNNN CTNNGCTNAT GANNNNNTNN NCCCCNGNCN TTTNNNNNNN NNN
//
Primer: SV40pro_F_V2 (Pr0154)
Region: NeoR_KanR
Sequence file: RDB17241_A9Eld.seq check
>D06280C5_A9El_2_SV40pro_F_V2_H03_24_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNGNN GNNNNNGCTT TTGNAAGNNC GATCNNGAGA NAGGATGAGG
   61 ANCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA
  121 GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT
  181 CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT
  241 GAATGAACTG CAAGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG
  301 CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT
  361 GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC
  421 TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC
  481 GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA
  541 TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGAG
  601 CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT
  661 GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG
  721 CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC
  781 TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA
  841 TCGCCTTCTT GACGANTTCT TCTGANNNGG ACTCTGGGGG TTCGAAATGA NCGACCNAGC
  901 GANGCCNNAC CTGCCNTCNC GANATTTCNA NTCCNCCGCC GCCNNNTATN AAAGGNTGGG
  961 CTTCNGNNNC GTTTNNCNGG NNNNNCNGNN NNGNNTGATN CNNCNNCGCG GGNNNNNCNN
1021 NNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Matsui, T., Small GTPase Rab12 regulates constitutive degradation of transferrin receptor. Traffic 12 (10): 1432-1443 (2011). PMID 21718402. [link to RRC of NBRP]
reference Fukuda, M., Distinct Rab binding specificity of Rim1, Rim2, rabphilin, and Noc2. Identification of a critical determinant of Rab3A/Rab27A recognition by Rim2. J. Biol. Chem. 278 (17): 15373-15380 (2003). PMID 12578829. [link to RRC of NBRP]