Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEGFP-C1-KIF13A-tail-deltaRHD

Expression vector of mouse KIF13A fused with EGFP. The KIF13A-tail mutant without a motor domain and the lacks an RHD, a Rab10-binding dificient mutant.

Catalog number RDB17232
Resource name pEGFP-C1-KIF13A-tail-deltaRHD
Clone info. Expression vector of mouse kinesin family member 13A (KIF13A) fused with EGFP at N-terminus. The KIF13A-tail mutant without a motor domain and the lacks an Rab10-binding homology domain (RHD), a Rab10-binding dificient mutant, see Fig. 4A and 5C of Etoh, K. and Fukuda, M., J. Cell Sci., 132 (5), jcs226977, 2019.
Expression was confirmed by the depositor with immunoblotting.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cell).
Gene/insert name mouse Kif13a cDNA
Depositor|Developer Fukuda, Mitsunori |
 
Other clones in our bank mouse Kif13a (NCBI Gene 16553) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ishida, M. et al. (2015) Sci. Rep. 5, 8238); (Etoh, K. and Fukuda, M. (2019) J. Cell Sci. 132, jcs226977)
Ordering Please visit Information of Request for Distribution.[link] 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Remarks
Please use Schedule A (GFP-A) additionally.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17232 pEGFP-C1-KIF13A-tail-deltaRHD DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Etoh, K., Rab10 regulates tubular endosome formation through KIF13A and KIF13B motors. J. Cell Sci. 132 (5). pii: jcs226977 (2019). PMID 30700496.

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17232_A9Elp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2
Region: EGFP 3', insert 5'
Sequence file: RDB17232_A9Ela.seq check
>D06280B5_A9El_1_EGFP-C2_B01_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNCGC NNNCNCNTGN NCCTGCTGGN NTTCGTGNNC GCCGCCGGGN
   61 TCNCTCTCGG CATGGNCGAN CTGTACAAGT CCGGACTCAG ATCCGACCCC AATGCCAAGG
  121 TCATCCGAGA GCTTCGAGAG GAAGTGGAGA AACTGAGGGA GCAGCTCTCG CAGGCTGAGG
  181 CCATGAAGGC CCCCGAGCTA AAGGAGAAGC TCGAAGAGTC TGAGAAGCTG ATAAAGGAGC
  241 TAACGGTGAC GTGGGAAGAG AAGCTGAGGA AAACAGAAGC GATCGCACAG GAGAGACAGC
  301 GGCAGCTGGA AAGCATGGGA ATCTCCCTGG AGACCTCTGG CATCAAGGTG GGGGACGACA
  361 AGTGCTACCT CGTCAACCTG AACGCAGACC CTGCCCTCAA CGAGCTTCTG GTTTACTATT
  421 TAAAGGATCA TACCCGGGTG GGTGCAGATA CCTCTCAGGA CATCCAGCTC TTCGGAATAG
  481 GGATTCAGCC CGAGCACTGT GAGATCGACA TCGCAGCGGA TGGAGACATC ACTCTCACGC
  541 CCAAAGAAAA TGCAAGGTCC TGTGTCAATG GCACCCTTGT CTGCAGTACT ACCCAGCTGT
  601 GGCACGGCGA CAGGATCTTA TGGGGAAACA ACCACTTTTT TAGAATTAAC TTGCCTAAGA
  661 GGAAACGGCG GGACTGGTTG AAAGACTTTG AGAGAGAAAN CAGTTCCGCG GAGCACGATC
  721 TAGACGCTGC GAGCGANGCT TCCTCGGANC CNGACTACNA CTACGANTTC GCACAGATGG
  781 ANGTCATCAT GAAAACACNG AACAGCANTG NTNCAGTTCA AAANGTGGTT CANGTCCNNN
841 NNAANCANTA CCNNNNNNNN NN
//
Primer: SV40poltA_R2
Region: insert 3'
Sequence file: RDB17232_A9Elb.seq check
>D06280B5_A9El_1_SV40polyA_R2_C01_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNGNNN NNNACAAGTT AACAACAACA ATTGNATTCA TTTTATGTTT
   61 CAGGTTCAGG GGGNGGTGTG GGAGGTTTTT TAAAGCAAGT AAAACCTCTA CAAATGTGGT
  121 ATGGCTGATT ATGATCAGTT ATCTAGATCC GGTGGATCCC GGGCCCGCGG TACCGTCGAC
  181 CTGCAGGCGG CCGCGGGAAT TCGATTTTAC CGAGTGGCTG TGGCTTCCTG GTCGTTCTCT
  241 ATCTTGGGAT GCAGCCGGTC GGCATCTGGA GGTCTTTCTG GGATACCTTT ATGGTCTGTT
  301 TCGTTTGTTA GAGCCCTCCT CCTGGAGGGC TCCCCTACAG AACAATCTGC CAAACCATCA
  361 AAGTCTTTGC CATCAGAAAC ACCCATGAAC TCTGTGAAGG AATGGTCCTC CAAAATGTTT
  421 GTAGTGTGTT CTATCGTCAC CTCTCCTGGG CAGAATTCTC TGGCTTGGTG GCCATCTTTG
  481 CTGGGGCCGA CATCCAGTTC TGAACATGAA GCAGTCCTGC ATGGCATTCT GGAACTTTCC
  541 TCAGGGATGC TCTGGGGTGA CGGGGTCCCC TTGGGTAAGG CACTGTTTTC CTCCAGCGGC
  601 ACAGCGATTG TCTCATCCTT CCCTGAGCCT CTTCCTACTT CTGTAAGCTC CTGATTCGAA
  661 GCTGGTCTGA CATCCGGTGC AAGTGATGGT CCCGGGGGCT CGGCGCACTC GACCTCCTTT
  721 GTCGAGACGG CAAGCTGGTC TGANCTGTCG CTNGNNGGGA NTGCCATGTC AGACAGAGTG
  781 NCATTGGANG CACTGNNGGA NAAGTANCCA CTNGNGNNGC TGCTGGNNGN ANGGCNACGG
  841 GNNANNNCTT NNTNNNNNNN TNTNGANTCT GNCNNNTCCN CCTTANAANN ANGNNNNNTC
901 CNNNNTNNTN CNANNNNNNN NNN
//
Primer: mKIF12A_F1
Region: insert mid
Sequence file: RDB17232_A9Elc.seq check
>D06280B5_A9El_1_mKIF13A_F14_D01_10_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN TGGCTGCGTG NNCGCCNGGT CCNCCAAGCT GCAGAGAGGG
   61 CTGGACAGCT ACCAGAGAGA TGAGGAGGAT GGCGGTGATA TGGATAGTTA TCAGGAAGAA
  121 GACTTGAATT GTGTAAGAGA GAGATGGTCC GATGCAGTGC TGGTGCCCGC CCCTGGCAGC
  181 GGAATCCCCG GGGCACCTGC TGACTGGGTC CCACCTCCTG GCATGGAAAC ACACATCCCG
  241 GTTCTCTTCC TGGATCTGAA CGCGGATGAC CTCAGTGCCA ATGAGCAGCT GGTGGGCCCC
  301 CATGCGTCCG GTGTGAACTC CATCCTGCCC AAGGAACACG GCAGTCAGTT TTTCTACCTG
  361 CCCATCATAA AGCACAGTGA TGACGAGGTT TCAGCCACAG CCTCCTGGGA CTCCTCTGTG
  421 CACGACTCTC TTCACCTGAA TCGGGTCACA CCGCAGAATG AAAGGATCTA CCTGATCGTG
  481 AAGACCACCG TGCAGCTCAG CCACCCGGCC GCCATGGAGC TAGTGTTGCG GAAGCGGATC
  541 GCGGCCAATA TTTATAACAA GCAGAGTTTC ACCCAGAGTT TGAAGAGACG AATATCACTG
  601 ATCAATATAT GTTACTCCTG CGGCGTGACC TATGAGATAG TATCCAATAT CCCAAAGGCG
  661 ACCGAGGAGA TTGAGGANCG GGAAACCCTG GCCCTCCTGG CAGCGCGGAG TGAGAACGAG
  721 GGCACTTTGG ATGGGGAGAC ATACATCGAG AAGTACACGC GGGGCGTGCT GCAGGTGGAG
  781 AATATCCTGA NCCTGGANCG GCTCCGGCAN GCTGTCACGG TGAANGAAGC GCTTTCCNCC
  841 AAAGCGAGGC ACATTCNGAN AANCCTCAGT ACANCGAACG TTCACNACGT CNCCTCTANC
  901 CNGNCNGATN NCTCNGGNTT CGATGAAGAN NANNNNNNCT GGNCNNANAN NNANNNANAN
961 NNGTCTGANN NCNGCTNCNN CTNNNNNGNN NNCNNNNNNN NNN
//
Primer: CMV_Forward
Region: CMV pro, EGFP 5'
Sequence file: RDB17232_A9Eld.seq check
>D06280B5_A9El_1_CMV_Forward_A01_01_ABI24.ab1
    1 NNNNNNNNNN NNNNNTGNNG CNNTGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
   61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGCTAC CGGTCGCCAC CATGGTGAGC
  121 AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA
  181 AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG
  241 ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC
  301 ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA GCAGCACGAC
  361 TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC
  421 GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC
  481 ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG
  541 TACAACTACA ACAGCCACAA CGTCTATATC ATGGCCGACA AGCAGAAGAA CGGCATCAAG
  601 GTGAACTTCA AGATCCGCCA CAACATCGAG GACGGCAGCG TGCAGCTCGC CGACCACTAC
  661 CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC
  721 ACCCAGTCCG CCCTGAGCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG
  781 TTCGTGACCG CCGCCGGGAT CACTCTCGGC ATGGNCGANC TGTACAAGTC CGGANTCAGA
  841 TCCGACCCCN ATGCCNAGGN CATCCGAGAN CTTCNAGAGG AAGTGGAGAA ANNNANGGAN
  901 CAGCTCTCGC ANGNNNANGC CNTGNANNNN CCCCNNNNCN NAAGGANANN CNCGAANANN
961 NTGNNNANNC TGANAAANGN ANCTNNNNGN NNNNNNNGGN ANNANNNNNN NNNN
//
Primer: SV40pro_ori_F
Region: SV40 pro_ori, NeoR_KanR
Sequence file: RDB17232_A9Ele.seq check
>D06280B5_A9El_1_SV40pro_ori_F_D01_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNG CCNCNGCCTC TGNNCTATTC NNGAAGTAGT GAGGANGCTT
   61 TTTTGGAGGC CTAGGCTTTT GCAAAGATCG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA
  121 TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG
  181 GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG
  241 CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC
  301 AAGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC
  361 TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG
  421 ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC
  481 GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA
  541 TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG
  601 AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGAGC ATGCCCGACG
  661 GCGANGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG
  721 GCCGCTTTTC TGGATTCATC GACNGNGGCC GGCTGGGNGT GGCGGANCGC TATCANGACA
  781 TAGCGTTGGC TACCCGNNAT ATNGCTGNNN NNCTNGGCNG CNAANGGGNN GANNGCTNNC
  841 TCNNNCTTNA NNGNATCGCC GCNCCNNATT CNCNNCNNNN CGCNNTNNNN NCNNCNNNNN
901 NNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Etoh, K., Rab10 regulates tubular endosome formation through KIF13A and KIF13B motors. J. Cell Sci. 132 (5). pii: jcs226977 (2019). PMID 30700496.
reference Ishida, M., Rab1A regulates anterograde melanosome transport by recruiting kinesin-1 to melanosomes through interaction with SKIP. Sci. Rep. 5: 8238 (2015). PMID 25649263.

2020.10.02

GNP_filter3_RDBDEP_html_200316.pl