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pEGFP-C1-KIF13A-tail-deltaRHD

Expression vector of mouse KIF13A fused with EGFP. The KIF13A-tail mutant without a motor domain and the lacks an RHD, a Rab10-binding dificient mutant, see Fig. 4A and 5C of Etoh, K. and Fukuda, M., J. Cell Sci., 132 (5), jcs226977, 2019.

Catalog number RDB17232
Resource name pEGFP-C1-KIF13A-tail-deltaRHD
Clone info. Expression vector of mouse kinesin family member 13A (KIF13A) fused with EGFP at N-terminus. The KIF13A-tail mutant without a motor domain and the lacks an Rab10-binding homology domain (RHD), a Rab10-binding dificient mutant, see Fig. 4A and 5C of Etoh, K. and Fukuda, M., J. Cell Sci., 132 (5), jcs226977, 2019.
Expression was confirmed by the depositor with immunoblotting.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cell).
Gene/insert name mouse Kif13a cDNA
Depositor Fukuda, Mitsunori |
 
Other clones in our bank mouse Kif13a (NCBI Gene 16553) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ishida, M. et al. (2015) Sci. Rep. 5, 8238); (Etoh, K. and Fukuda, M. (2019) J. Cell Sci. 132, jcs226977)
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Schedule A (GFP-A) [Link]
Remarks
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Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB17232 pEGFP-C1-KIF13A-tail-deltaRHD Under QC test. Please contact us. DNA solution

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References and tips

Electronic file

Original reference

original Etoh, K., Rab10 regulates tubular endosome formation through KIF13A and KIF13B motors. J. Cell Sci. 132 (5). pii: jcs226977 (2019). PMID 30700496.

Further references such as user reports and related articles (go to bottom)

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Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

Original, user report and related articles

original Etoh, K., Rab10 regulates tubular endosome formation through KIF13A and KIF13B motors. J. Cell Sci. 132 (5). pii: jcs226977 (2019). PMID 30700496.
reference Ishida, M., Rab1A regulates anterograde melanosome transport by recruiting kinesin-1 to melanosomes through interaction with SKIP. Sci. Rep. 5: 8238 (2015). PMID 25649263.

2019.07.17

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