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pEF-T7-mALS2-D1593A

Expression vector of mouse ALS2, a GEF-activity-deficient mutant, D1593A, tagged with T7-tag.

Catalog number RDB17220
Resource name pEF-T7-mALS2-D1593A
Clone info. Expression vector of mouse alsin Rho guanine nucleotide exchange factor (ALS2), a Rab5-guanine-nucleotide exchange factor (GEF)-activity-deficient mutant, D1593A, tagged with N-terminal T7, EF1 promoter.
Expression was confirmed by the depositor with immunoblotting.
Vector backbone pEF-T7 (plasmid)
Selectable markers Amp^r
Gene/insert name mouse Als2 cDNA
Depositor Fukuda, Mitsunori |
 
Other clones in our bank mouse Als2 (NCBI Gene 74018) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Mori, Y. et al. (2013) J. Biol. Chem. 288, 9835-9847); (Morishita, S. et al. (2019) FEBS Lett. 593, 230-241)
Additional terms and conditions for distribution Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the pEF-BOS and pEF-BOS-EX vectors (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
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Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB17220 pEF-T7-mALS2-D1593A Under QC test. Please contact us. DNA solution

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References and tips

Electronic file

Original reference

original Morishita, S., Rab5 activation on macropinosomes requires ALS2, and subsequent Rab5 inactivation through ALS2 detachment requires active Rab7. FEBS Lett. 593 (2): 230-241 (2019). PMID 30485418.

Further references such as user reports and related articles (go to bottom)

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Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

Original, user report and related articles

original Morishita, S., Rab5 activation on macropinosomes requires ALS2, and subsequent Rab5 inactivation through ALS2 detachment requires active Rab7. FEBS Lett. 593 (2): 230-241 (2019). PMID 30485418.
reference Mori, Y., Rabex-5 protein regulates dendritic localization of small GTPase Rab17 and neurite morphogenesis in hippocampal neurons. J. Biol. Chem. 288 (14): 9835-9847 (2013). PMID 23430262.

2019.07.17

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