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pEF-T7-mALS2-D1593A (#RDB17220)

Expression vector of mouse ALS2, a GEF-activity-deficient mutant, D1593A, tagged with T7-tag.

Clone info. Expression vector of mouse alsin Rho guanine nucleotide exchange factor (ALS2), a Rab5-guanine-nucleotide exchange factor (GEF)-activity-deficient mutant, D1593A, tagged with N-terminal T7, EF1 promoter.
Comment Expression was confirmed by the depositor with immunoblotting.
Vector backbone pEF-T7 (plasmid)
Selectable markers Amp^r
Gene/insert name mouse Als2 cDNA
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Mori, Y. et al. (2013) J. Biol. Chem. 288, 9835-9847); (Morishita, S. et al. (2019) FEBS Lett. 593, 230-241)
Additional terms and conditions:
Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the pEF-BOS and pEF-BOS-EX vectors (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Mori, Y. et al. (2013) J. Biol. Chem. 288, 9835-9847); (Morishita, S. et al. (2019) FEBS Lett. 593, 230-241)。
MTAに書く付加的使用条件：
pEF-BOS and pEF-BOS-EXベクタについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクタを商業的に利用する場合、利用者は長田重一博士に連絡するものとする。利用者は、研究成果の公表にあたってpEF-BOSおよびpEF-BOS-EXベクタの文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990)を引用し、長田重一教授への謝辞の表明を必要とする。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB17220 pEF-T7-mALS2-D1593A Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pEF-T7-mALS2-D1593A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17220).

Reference section:

Morishita, S., Wada, N., Fukuda, M., Nakamura, T., Rab5 activation on macropinosomes requires ALS2, and subsequent Rab5 inactivation through ALS2 detachment requires active Rab7. FEBS Lett. 593 (2): 230-241 (2019). PMID 30485418. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Morishita, S., Rab5 activation on macropinosomes requires ALS2, and subsequent Rab5 inactivation through ALS2 detachment requires active Rab7. FEBS Lett. 593 (2): 230-241 (2019). PMID 30485418. [link to RRC of NBRP]
reference Mori, Y., Rabex-5 protein regulates dendritic localization of small GTPase Rab17 and neurite morphogenesis in hippocampal neurons. J. Biol. Chem. 288 (14): 9835-9847 (2013). PMID 23430262. [link to RRC of NBRP]