Expression vector of mouse ALS2, a GEF-activity-deficient mutant, D1593A, tagged with T7-tag.
Catalog number | RDB17220 |
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Resource name | pEF-T7-mALS2-D1593A |
Clone info. | Expression vector of mouse alsin Rho guanine nucleotide exchange factor (ALS2), a Rab5-guanine-nucleotide exchange factor (GEF)-activity-deficient mutant, D1593A, tagged with N-terminal T7, EF1 promoter. Expression was confirmed by the depositor with immunoblotting. |
Vector backbone | pEF-T7 (plasmid) |
Selectable markers | Amp^r |
Gene/insert name | mouse Als2 cDNA |
Depositor | Fukuda, Mitsunori | |
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Other clones in our bank | mouse Als2 (NCBI Gene 74018) | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Mori, Y. et al. (2013) J. Biol. Chem. 288, 9835-9847); (Morishita, S. et al. (2019) FEBS Lett. 593, 230-241) |
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Additional terms and conditions for distribution | Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the pEF-BOS and pEF-BOS-EX vectors (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested. |
Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
Catalog # | Resource name | Availability | Shipping form | Fee (non-profit org.) |
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RDB17220 | pEF-T7-mALS2-D1593A | Under QC test. Please contact us. | DNA solution |
Electronic file
Original reference
Further references such as user reports and related articles (go to bottom)
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This clone will be sequenced a portion and digested by restriction enzyme for examination.
Original, user report and related articles
original | Morishita, S., Rab5 activation on macropinosomes requires ALS2, and subsequent Rab5 inactivation through ALS2 detachment requires active Rab7. FEBS Lett. 593 (2): 230-241 (2019). PMID 30485418. |
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reference | Mori, Y., Rabex-5 protein regulates dendritic localization of small GTPase Rab17 and neurite morphogenesis in hippocampal neurons. J. Biol. Chem. 288 (14): 9835-9847 (2013). PMID 23430262. |
2019.07.17
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