RDB17220 - pEF-T7-mALS2-D1593A

Expression vector of mouse ALS2, a GEF-activity-deficient mutant, D1593A, tagged with T7-tag.

Distribution information

Catalog number	RDB17220
Resource name	pEF-T7-mALS2-D1593A
Clone info.	Expression vector of mouse alsin Rho guanine nucleotide exchange factor (ALS2), a Rab5-guanine-nucleotide exchange factor (GEF)-activity-deficient mutant, D1593A, tagged with N-terminal T7, EF1 promoter. Expression was confirmed by the depositor with immunoblotting.
Vector backbone	pEF-T7 (plasmid)
Selectable markers	Amp^r
Gene/insert name	mouse Als2 cDNA
Depositor	Fukuda, Mitsunori \|

Other clones in our bank	mouse Als2 (NCBI Gene 74018) \|

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Mori, Y. et al. (2013) J. Biol. Chem. 288, 9835-9847); (Morishita, S. et al. (2019) FEBS Lett. 593, 230-241)
Additional terms and conditions for distribution	Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the pEF-BOS and pEF-BOS-EX vectors (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
Ordering	Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word]

Distribution information (domestic users)

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Mori, Y. et al. (2013) J. Biol. Chem. 288, 9835-9847); (Morishita, S. et al. (2019) FEBS Lett. 593, 230-241)。
付加的提供条件	pEF-BOS and pEF-BOS-EXベクタについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクタを商業的に利用する場合、利用者は長田重一博士に連絡するものとする。利用者は、研究成果の公表にあたってpEF-BOSおよびpEF-BOS-EXベクタの文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990)を引用し、長田重一教授への謝辞の表明を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて」をご覧ください。提供依頼書 [Word] 提供同意書 [Word]

References and tips

Sequence information

Catalog #	Resource name	Availability	Shipping form	Fee (non-profit org.)
RDB17220	pEF-T7-mALS2-D1593A	Under QC test. Please contact us.	DNA solution

original	Morishita, S., Rab5 activation on macropinosomes requires ALS2, and subsequent Rab5 inactivation through ALS2 detachment requires active Rab7. FEBS Lett. 593 (2): 230-241 (2019). PMID 30485418.

This clone will be sequenced a portion and digested by restriction enzyme for examination.

original	Morishita, S., Rab5 activation on macropinosomes requires ALS2, and subsequent Rab5 inactivation through ALS2 detachment requires active Rab7. FEBS Lett. 593 (2): 230-241 (2019). PMID 30485418.
reference	Mori, Y., Rabex-5 protein regulates dendritic localization of small GTPase Rab17 and neurite morphogenesis in hippocampal neurons. J. Biol. Chem. 288 (14): 9835-9847 (2013). PMID 23430262.