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pEGFP-C1-Rab12-Q100L (#RDB16882)

Expression vector of mouse Rab12A constitutive active mutant, Q100L, fused with EGFP at N-terminus.

Clone info. Expression vector of mouse Rab12A constitutive active mutant, Q100L, fused with EGFP at N-terminus.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name mouse Rab12 cDNA
Reference of insert sequence AB232607 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

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Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Fukuda, M. J. Biol. Chem. 278, 15373-15380, 2003); (Itoh, T. et al. Genes Cells 11, 1023-1037, 2006)
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提供依頼書  [依頼書の記入例 ]
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Fukuda, M. J. Biol. Chem. 278, 15373-15380, 2003); (Itoh, T. et al. Genes Cells 11, 1023-1037, 2006)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16882 pEGFP-C1-Rab12-Q100L DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-Rab12-Q100L was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16882).

Reference section:

Ishida, M., Ohbayashi, N., Maruta, Y., Ebata, Y., Fukuda, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci., 125 (Pt 21): 5177-5187 (2012). PMID 22854043. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16882_A9A8p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-N_F2 (Pr1228)
Region: EGFP 3',insert 5'
Sequence file: RDB16882_A9A8a.seq check
>D06060G2_A9A8_3_EGFP-N_F2_B01_04_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNGCN NCNTCTTCTT CAGGNNGACG GCAACTACNA GACCCGCGCC
   61 GAGGTGAAGT TCGANGGCGA CACCCTGGTG AACCGCATCG AGCTGAAGGG CATCGACTTC
  121 AAGGAGGACG GCAACATCCT GGGGCACAAG CTGGAGTACA ACTACAACAG CCACAACGTC
  181 TATATCATGG CCGACAAGCA GAAGAACGGC ATCAAGGTGA ACTTCAAGAT CCGCCACAAC
  241 ATCGAGGACG GCAGCGTGCA GCTCGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC
  301 GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCACCC AGTCCGCCCT GAGCAAAGAC
  361 CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG TGACCGCCGC CGGGATCACT
  421 CTCGGCATGG ACGAGCTGTA CAAGTCCGGA CTCAGATCTA TGGATCCCAG CGCCGCGCTG
  481 CACCGACGGC CCGCGGGCGG CAGCCTGGGC GCGGTCTCCC CGGCGCTGTC GGGCGGCCAG
  541 GCCCGCCGGA GGAAGCAGCC CCCCAGGCCG GCCGACTTCA AGCTGCAGGT CATCATCATC
  601 GGCTCCCGCG GCGTGGGCAA GACCAGCCTG ATGGAGCGCT TCACCGACGA CACGTTCTGC
  661 GAGGCCTGCA AGTCCACCGT GGGTGTTGAC TTTAAAATCA AGACTGTAGA ACTAAGAGGG
  721 AAGANNATTA GATTACNGAT CTGGGACACA GCANGGCTGG ANCGATTTAN CAGCATTACC
  781 TCGGCCTATT ACAGAAGTGN CAAGGGGNNC ATACTCGTAT ATGACATCAC CNAGAANNNA
  841 GANGTTCNAT GACTNGNCNA AGNGNANGAA NATGANNGAC NAGNATGCTT CAGAANANGC
  901 CNANCTNNNN NNNNNTNGNA AANNNNCTNN NNNNNGANAN NNNNNGANAA NTCTCNNNNN
961 NNNNN
//
Primer: SV40polyA_R2 (Pr0623)
Region: insert 3'
Sequence file: RDB16882_A9A8b.seq check
>D06060G2_A9A8_3_SV40polyA-R2_F08_17_ABI24.ab1
    1 NNNNNNNNNN CNNNNNNNNN AGTTAACNNN NACNATTGCA TTCNTTTTAT GTTTCNGGTT
   61 CAGGGGGAGG TGTGGGAGGT TTTTTAAAGC AAGTAAAACC TCTACAAATG TGGTATGGCT
  121 GATTATGATC AGTTATCTAG ATCCGGTGGA TCCCGGGCCC GCGGTACCGT CGACTGCAGA
  181 ATTCACTAGT GATTCTCCAA AGTATCAAAT CAACAGCATC GGACGTGTGG TCTGGGAGGA
  241 GGCAGCTCTG GTGGGATCTC AGGCTCTGGT TGTAGAGAAA GGATGCTATT GGATAACTCG
  301 TTTCTCAAAA CATCAAGAGG CATCTTTTTC AGAATATCAT CAACGAGTTT CAGAAAGATC
  361 TCGTCCACAT TGAAATTGTC CTTGGCACTG GCTTCGCAGA AGCGCATCCC GGTTATCTGC
  421 TGTGCAAACT TTTCTCCTTG CTGCCTTGAG ATTTCTCTGT CTGTCTCACA GTCCAGCTTA
  481 TTTCCAACCA GGAGGAGCTC GGCGTCTTCT GAAGCATACT TGTCAATCAT CTTCATCCAC
  541 TTTGGCAAGT CATCGAACGT CTCCTTCTTG GTGATGTCAT ATACGAGTAT GATCCCCTTG
  601 GCACTTCTGT AATAGGCCGA GGTAATGCTG TTAAATCGCT CCAGCCCTGC TGTGTCCCAG
  661 ATCTGTAATC TAATTTTCTT CCCTCTTAGT TCTACAGTCT TGATTTTAAA GTCAACACCC
  721 ACGGTGGACT TGCAGGCCTC GCAGAACGTG TCGTCGGTGA AGCGCTCCAT CAGGCTGGTC
  781 TTGCCCACGC CGCGGGANCC GATGANGANG ANCTGCAGCT TGAAGTCGGN CGGCCTGGGG
  841 GGCTGCTTCC TCCGGCGGGC CTGGCCGCCC GACAGCGCCN GGNANNNCGC GCCCAGGCTG
  901 NCGCCNGCGG GCCGNCNGNG CAGCGCGGCG CTNGGATNNN TANATCTGAN TNCGGANTTN
  961 NNCAGCTCGT CCNTGNCGAN ANTGNTNCNN GNGGNGGNCN NNANNNNNNN CNNNANNNTG
1021 NNNTNNCNNT NNNNNNNGGG GNCTTNGNNN NNNNNGNNNT NNNNNNNNNN
//
Primer: CMV_Forward (Pr0016)
Region: CMV pro,EGFP 5'
Sequence file: RDB16882_A9A8c.seq check
>D06060G2_A9A8_3_CMV_Forward_G08_20_ABI24.ab1
    1 NNNNNNNNNN NNNNTGNNGC NNTGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC
   61 AGAGCTGGTT TAGTGAACCG TCAGATCCGC TAGCGCTACC GGTCGCCACC ATGGTGAGCA
  121 AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA
  181 ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA
  241 CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA
  301 CCCTGACCTA CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT
  361 TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG
  421 ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA
  481 TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT
  541 ACAACTACAA CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC GGCATCAAGG
  601 TGAACTTCAA GATCNNNNNN AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC
  661 AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCA
  721 CCCAGTCCGC CCTGAGCAAA GANCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT
  781 TCGTGANCGC CGCCGGGATC ACTCTCGGCA TGGNCGANCT GTACNAGTCC GGANTCANAT
  841 CTATGGATCC CAGCGCCGCG CTGCACCGAC NGCCCGCGGG CGGCAGCCNG GGCGCGGNCT
  901 NCCNGGNGCT GNCNGGCGGN CNGNNNNGNN NGANGAANNA GCCNNCNNNG NNNGNNGANT
  961 TNNNGCTGCN NNNCNTCNTC NTCGGNTCCN NNNNNNNNGN NNNNANNNNN NNGNNNGNAN
1021 CNNNTNNNNN NNNNN
//
Primer: SV40pro_F_V2 (Pr0154)
Region: NeoR_KanR
Sequence file: RDB16882_A9A8d.seq check
>D06060G2_A9A8_3_SV40pro_F_V2_H08_23_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNCNN NGCTTTTGNA AGANCGATCN AGANGACAGG NTGAGGATCG
   61 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG
  121 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG
  181 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT
  241 GAACTGCAAG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA
  301 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG
  361 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT
  421 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA
  481 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG
  541 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGAGCATG
  601 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG
  661 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGANCGCTAT
  721 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGANC TTGGCGGCGA ATGGGCTGAC
  781 CGCTTCCTCG TGCTTTACGG TATCGCCGCN NCCGATTCGC AGCGCATCGC CTTCTATCGC
  841 CNTCNTGANG ANTTCTTCTG ANCGGGNNTC TGGGGNTCGA ANTGANCGAN NNAGCGANGC
  901 CCNANCTGNC NTCNCGANNN TTCNANTNCN CNNNNNNCTT CTATNNAANG NNTNGNCTTC
  961 NGNNNCNTTT TNNNGNNNNN CNGGNNNNNN GATNNNNNNN CNNGGGGNNN NCNNGNTNGN
1021 NNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ishida, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci., 125 (Pt 21): 5177-5187 (2012). PMID 22854043. [link to RRC of NBRP]
reference Itoh, T., Screening for target Rabs of TBC (Tre-2/Bub2/Cdc16) domain-containing proteins based on their Rab-binding activity. Genes Cells 11 (9): 1023-1037 (2006). PMID 16923123. [link to RRC of NBRP]
reference Fukuda, M., Distinct Rab binding specificity of Rim1, Rim2, rabphilin, and Noc2. Identification of a critical determinant of Rab3A/Rab27A recognition by Rim2. J. Biol. Chem. 278 (17): 15373-15380 (2003). PMID 12578829. [link to RRC of NBRP]