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pEGFP-C1-Rab12-Q100L

Expression vector of mouse Rab12A constitutive active mutant, Q100L, fused with EGFP at N-terminus.

Catalog number RDB16882
Resource name pEGFP-C1-Rab12-Q100L
Clone info. Expression vector of mouse Rab12A constitutive active mutant, Q100L, fused with EGFP at N-terminus.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name mouse Rab12 cDNA
Reference of insert sequence AB232607 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
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External Database
mouse Rab12

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Fukuda, M. J. Biol. Chem. 278, 15373-15380, 2003); (Itoh, T. et al. Genes Cells 11, 1023-1037, 2006)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Fukuda, M. J. Biol. Chem. 278, 15373-15380, 2003); (Itoh, T. et al. Genes Cells 11, 1023-1037, 2006)。
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          RDB16882 pEGFP-C1-Rab12-Q100L DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pEGFP-C1-Rab12-Q100L was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16882).

          Reference section:

          Ishida, M., Ohbayashi, N., Maruta, Y., Ebata, Y., Fukuda, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci., 125 (Pt 21): 5177-5187 (2012). PMID 22854043. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)


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          Featured content

          Featured content Rab family protein (English text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16882_A9A8p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EGFP-N_F2 (Pr1228)
          Region: EGFP 3',insert 5'
          Sequence file: RDB16882_A9A8a.seq check
          >D06060G2_A9A8_3_EGFP-N_F2_B01_04_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNGCN NCNTCTTCTT CAGGNNGACG GCAACTACNA GACCCGCGCC
             61 GAGGTGAAGT TCGANGGCGA CACCCTGGTG AACCGCATCG AGCTGAAGGG CATCGACTTC
            121 AAGGAGGACG GCAACATCCT GGGGCACAAG CTGGAGTACA ACTACAACAG CCACAACGTC
            181 TATATCATGG CCGACAAGCA GAAGAACGGC ATCAAGGTGA ACTTCAAGAT CCGCCACAAC
            241 ATCGAGGACG GCAGCGTGCA GCTCGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC
            301 GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCACCC AGTCCGCCCT GAGCAAAGAC
            361 CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG TGACCGCCGC CGGGATCACT
            421 CTCGGCATGG ACGAGCTGTA CAAGTCCGGA CTCAGATCTA TGGATCCCAG CGCCGCGCTG
            481 CACCGACGGC CCGCGGGCGG CAGCCTGGGC GCGGTCTCCC CGGCGCTGTC GGGCGGCCAG
            541 GCCCGCCGGA GGAAGCAGCC CCCCAGGCCG GCCGACTTCA AGCTGCAGGT CATCATCATC
            601 GGCTCCCGCG GCGTGGGCAA GACCAGCCTG ATGGAGCGCT TCACCGACGA CACGTTCTGC
            661 GAGGCCTGCA AGTCCACCGT GGGTGTTGAC TTTAAAATCA AGACTGTAGA ACTAAGAGGG
            721 AAGANNATTA GATTACNGAT CTGGGACACA GCANGGCTGG ANCGATTTAN CAGCATTACC
            781 TCGGCCTATT ACAGAAGTGN CAAGGGGNNC ATACTCGTAT ATGACATCAC CNAGAANNNA
            841 GANGTTCNAT GACTNGNCNA AGNGNANGAA NATGANNGAC NAGNATGCTT CAGAANANGC
            901 CNANCTNNNN NNNNNTNGNA AANNNNCTNN NNNNNGANAN NNNNNGANAA NTCTCNNNNN
          961 NNNNN
          //
          Primer: SV40polyA_R2 (Pr0623)
          Region: insert 3'
          Sequence file: RDB16882_A9A8b.seq check
          >D06060G2_A9A8_3_SV40polyA-R2_F08_17_ABI24.ab1
              1 NNNNNNNNNN CNNNNNNNNN AGTTAACNNN NACNATTGCA TTCNTTTTAT GTTTCNGGTT
             61 CAGGGGGAGG TGTGGGAGGT TTTTTAAAGC AAGTAAAACC TCTACAAATG TGGTATGGCT
            121 GATTATGATC AGTTATCTAG ATCCGGTGGA TCCCGGGCCC GCGGTACCGT CGACTGCAGA
            181 ATTCACTAGT GATTCTCCAA AGTATCAAAT CAACAGCATC GGACGTGTGG TCTGGGAGGA
            241 GGCAGCTCTG GTGGGATCTC AGGCTCTGGT TGTAGAGAAA GGATGCTATT GGATAACTCG
            301 TTTCTCAAAA CATCAAGAGG CATCTTTTTC AGAATATCAT CAACGAGTTT CAGAAAGATC
            361 TCGTCCACAT TGAAATTGTC CTTGGCACTG GCTTCGCAGA AGCGCATCCC GGTTATCTGC
            421 TGTGCAAACT TTTCTCCTTG CTGCCTTGAG ATTTCTCTGT CTGTCTCACA GTCCAGCTTA
            481 TTTCCAACCA GGAGGAGCTC GGCGTCTTCT GAAGCATACT TGTCAATCAT CTTCATCCAC
            541 TTTGGCAAGT CATCGAACGT CTCCTTCTTG GTGATGTCAT ATACGAGTAT GATCCCCTTG
            601 GCACTTCTGT AATAGGCCGA GGTAATGCTG TTAAATCGCT CCAGCCCTGC TGTGTCCCAG
            661 ATCTGTAATC TAATTTTCTT CCCTCTTAGT TCTACAGTCT TGATTTTAAA GTCAACACCC
            721 ACGGTGGACT TGCAGGCCTC GCAGAACGTG TCGTCGGTGA AGCGCTCCAT CAGGCTGGTC
            781 TTGCCCACGC CGCGGGANCC GATGANGANG ANCTGCAGCT TGAAGTCGGN CGGCCTGGGG
            841 GGCTGCTTCC TCCGGCGGGC CTGGCCGCCC GACAGCGCCN GGNANNNCGC GCCCAGGCTG
            901 NCGCCNGCGG GCCGNCNGNG CAGCGCGGCG CTNGGATNNN TANATCTGAN TNCGGANTTN
            961 NNCAGCTCGT CCNTGNCGAN ANTGNTNCNN GNGGNGGNCN NNANNNNNNN CNNNANNNTG
          1021 NNNTNNCNNT NNNNNNNGGG GNCTTNGNNN NNNNNGNNNT NNNNNNNNNN
          //
          Primer: CMV_Forward (Pr0016)
          Region: CMV pro,EGFP 5'
          Sequence file: RDB16882_A9A8c.seq check
          >D06060G2_A9A8_3_CMV_Forward_G08_20_ABI24.ab1
              1 NNNNNNNNNN NNNNTGNNGC NNTGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC
             61 AGAGCTGGTT TAGTGAACCG TCAGATCCGC TAGCGCTACC GGTCGCCACC ATGGTGAGCA
            121 AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA
            181 ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA
            241 CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA
            301 CCCTGACCTA CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT
            361 TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG
            421 ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA
            481 TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT
            541 ACAACTACAA CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC GGCATCAAGG
            601 TGAACTTCAA GATCNNNNNN AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC
            661 AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCA
            721 CCCAGTCCGC CCTGAGCAAA GANCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT
            781 TCGTGANCGC CGCCGGGATC ACTCTCGGCA TGGNCGANCT GTACNAGTCC GGANTCANAT
            841 CTATGGATCC CAGCGCCGCG CTGCACCGAC NGCCCGCGGG CGGCAGCCNG GGCGCGGNCT
            901 NCCNGGNGCT GNCNGGCGGN CNGNNNNGNN NGANGAANNA GCCNNCNNNG NNNGNNGANT
            961 TNNNGCTGCN NNNCNTCNTC NTCGGNTCCN NNNNNNNNGN NNNNANNNNN NNGNNNGNAN
          1021 CNNNTNNNNN NNNNN
          //
          Primer: SV40pro_F_V2 (Pr0154)
          Region: NeoR_KanR
          Sequence file: RDB16882_A9A8d.seq check
          >D06060G2_A9A8_3_SV40pro_F_V2_H08_23_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNCNN NGCTTTTGNA AGANCGATCN AGANGACAGG NTGAGGATCG
             61 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG
            121 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG
            181 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT
            241 GAACTGCAAG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA
            301 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG
            361 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT
            421 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA
            481 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG
            541 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGAGCATG
            601 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG
            661 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGANCGCTAT
            721 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGANC TTGGCGGCGA ATGGGCTGAC
            781 CGCTTCCTCG TGCTTTACGG TATCGCCGCN NCCGATTCGC AGCGCATCGC CTTCTATCGC
            841 CNTCNTGANG ANTTCTTCTG ANCGGGNNTC TGGGGNTCGA ANTGANCGAN NNAGCGANGC
            901 CCNANCTGNC NTCNCGANNN TTCNANTNCN CNNNNNNCTT CTATNNAANG NNTNGNCTTC
            961 NGNNNCNTTT TNNNGNNNNN CNGGNNNNNN GATNNNNNNN CNNGGGGNNN NCNNGNTNGN
          1021 NNNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Ishida, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci., 125 (Pt 21): 5177-5187 (2012). PMID 22854043. [link to RRC of NBRP]
          reference Itoh, T., Screening for target Rabs of TBC (Tre-2/Bub2/Cdc16) domain-containing proteins based on their Rab-binding activity. Genes Cells 11 (9): 1023-1037 (2006). PMID 16923123. [link to RRC of NBRP]
          reference Fukuda, M., Distinct Rab binding specificity of Rim1, Rim2, rabphilin, and Noc2. Identification of a critical determinant of Rab3A/Rab27A recognition by Rim2. J. Biol. Chem. 278 (17): 15373-15380 (2003). PMID 12578829. [link to RRC of NBRP]

          2024.03.26

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