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pGEX-4T-3-Rab40A (#RDB16862)

Expression vector of human Rab40A tagged with GST at N-terminus, tac promoter.

Clone info. Expression vector of human Rab40A tagged with GST at N-terminus, tac promoter.
Comment Expression was confirmed by the depositor with immunoblotting.
Vector backbone pGEX-4T-3 (plasmid)
Selectable markers Amp^r
Gene/insert name human RAB40A cDNA
Reference of insert sequence AB232637 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
 

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Itoh, T. et al. Genes Cells 11, 1023-1037, 2006)
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Itoh, T. et al. Genes Cells 11, 1023-1037, 2007)。

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RDB16862 pGEX-4T-3-Rab40A DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGEX-4T-3-Rab40A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16862).

Reference section:

Itoh, T., Satoh, M., Kanno, E., Fukuda, M., Screening for target Rabs of TBC (Tre-2/Bub2/Cdc16) domain-containing proteins based on their Rab-binding activity. Genes Cells 11 (9): 1023-1037 (2006). PMID 16923123. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16862_A9A8p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGEX-F2 (Pr0403)
Region: GST,thrombin site,insert 5'
Sequence file: RDB16862_A9A8a.seq check
>D06060D9_A9A8_2_pGEX-F2_A01_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNTATTG AAGCTATCCC NCAAATTGAT AAGTACTTGA
   61 AATCCAGCAA GTATATAGCA TGGCCTTTGC AGGGCTGGCA AGCCACGTTT GGTGGTGGCG
  121 ACCATCCTCC AAAATCGGAT CTGGTTCCGC GTGGATCCAT GAGCGCCCCG GGCAGCCCCG
  181 ACCAGGCCTA TGACTTCCTG CTCAAGTTCC TGCTGGTGGG CGACAGGGAC GTAGGCAAGA
  241 GTGAGATCCT GGAGAGCCTG CAGGATGGTG CAGCTGAGTC CCCGTACAGC CATCTCGGGG
  301 GGATCGACTA CAAGACGACC ACCATCCTGC TGGACGGCCA GCGGGTGAAG CTGAAGCTCT
  361 GGGATACGTC GGGGCAGGGA AGATTTTGTA CCATATTCCG CTCCTACTCT CGTGGTGCAC
  421 AAGGAGTGAT CCTGGTCTAC GACATTGCAA ACCGCTGGTC TTTCGAGGGT ATGGATCGAT
  481 GGATTAAGAA GATTGAGGAA CATGCCCCTG GTGTCCCTAA AATCCTGGTG GGGAATCGCC
  541 TACATCTGGC ATTCAAGAGG CAGGTGCCCA GGGAGCAGGC CCAGGCCTAC GCCGAGCGCC
  601 TGGGTGTGAC CTTCTTTGAG GTCAGCCCTC TGTGCAATTT CAACATCATA GAGTCTTTCA
  661 CGGAGCTGGC CAGGATAGTG CTGCTGCGGC ACAGGATGAA TTGGCTCGGG AGGCCGAGCA
  721 AGGTACTGAG CTTGCAAGAC CTCTGCTGCC GCACCATCGT GTCCTGCACA CCTGTGCATC
  781 TGGTGGANAA GCTCCCGCTC CNCNNTACCT TAAGAAGCCA CCTCAAGTCC TTCTCCATGG
  841 CTAANGGCCT GAATGCCAGG ATGANGCGAG GCCTCTCCTA CTCCCTCACC NNCAGCTCCA
  901 CTCACAAGAN CAGCCTCTGC AAAGTGGANA TCGNCTGNCC ACCCCNNANN CCACCCNAAA
  961 NNTGNNCCAG AAACAGCTGC NAAANTTCTT NNNNANNNNN NNNAAANNAA ANNNNNNNGN
1021 NNNNNNNNN
//
Primer: pJB8-Forward (Pr0084)
Region: insert 3'
Sequence file: RDB16862_A9A8b.seq check
>D06060D9_A9A8_2_pJB8-Forward_D01_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNTNNN GGCTGCGCCC CGACACCCGC CAACACCCGC
   61 TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT
  121 CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGAT
  181 CGTCAGTCAG TCACGATGCG GCCGCGGGAA TTCGATTATT CCAGCTTGTT TCCTTTTGGT
  241 GCCTTCCTTA AGAAATTTTG CAGCTGTTTC TGGTGCAGTT TTTGGGTGGG CTCTGGGGTG
  301 GGCAGACGAT CTCCACTTTG CAGAGGCTGC TCTTGTGAGT GGAGCTGGTG GTGAGGGAGT
  361 AGGAGAGGCC TCGCATCATC CTGGCATTCA GGCCCTTAGC CATGGAGAAG GACTTGAGGT
  421 GGCTTCTTAA GGTACTGGGG AGCGGGAGCT TGTCCACCAG ATGCACAGGT GTGCAGGACA
  481 CGATGGTGCG GCAGCAGAGG TCTTGCAAGC TCAGTACCTT GCTCGGCCTC CCGAGCCAAT
  541 TCATCCTGTG CCGCAGCAGC ACTATCCTGG CCAGCTCCGT GAAAGACTCT ATGATGTTGA
  601 AATTGCACAG AGGGCTGACC TCAAAGAAGG TCACACCCAG GCGCTCGGCG TAGGCCTGGG
  661 CCTGCTCCCT GGGCACCTGC CTCTTGAATG CCAGATGTAG GCGATTCCCC ACCAGGATTT
  721 TAGGGACACC AGGGGCATGT TCCTCAATCT TCTTAATCCA TCGNTCCATA CCCTCGAAAG
  781 ACCNGCGGTT TGCAATGTCG TANANCAGGN TCACTCCTTG TGCACCACGA GAGTANGANC
  841 GGAATATGGT ACAAAATCTT CCNTGCCCCG ANGTATNCCN NNNNNTCNGC TTCACCCGCT
  901 GGNCGTNCAG CANGANGGTG GNCGNCTTGT ANNNNATNCC CCCNANNNGG NTGTNNNGGG
  961 ANTCNNCTGC NCCNNNCNGN NNNNTCTNCN NNATNNNNNT CTNNNNNANN NNCNNGNCGC
1021 CNNNNANCNN NNANNTNNAN NNNGNANGNN ATNNNNNNNN NN
//
Primer: pGEX-ptac_up-F (Pr0455)
Region: tac pro,lacO,GST,insert 5'
Sequence file: RDB16862_A9A8c.seq check
>D06060D9_A9A8_2_pGEX-ptac_up-F_B01_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NGTTCTGGNA ATATTCTGAA NTGAGCTGTT GANAATTAAT
   61 CATCGGCTCG TATAATGTGT GGAATTGTGA GCGGATAACA ATTTCACACA GGAAACAGTA
  121 TTCATGTCCC CTATACTAGG TTATTGGAAA ATTAAGGGCC TTGTGCAACC CACTCGACTT
  181 CTTTTGGAAT ATCTTGAAGA AAAATATGAA GAGCATTTGT ATGAGCGCGA TGAAGGTGAT
  241 AAATGGCGAA ACAAAAAGTT TGAATTGGGT TTGGAGTTTC CCAATCTTCC TTATTATATT
  301 GATGGTGATG TTAAATTAAC ACAGTCTATG GCCATCATAC GTTATATAGC TGACAAGCAC
  361 AACATGTTGG GTGGTTGTCC AAAAGAGCGT GCAGAGATTT CAATGCTTGA AGGAGCGGTT
  421 TTGGATATTA GATACGGTGT TTCGAGAATT GCATATAGTA AAGACTTTGA AACTCTCAAA
  481 GTTGATTTTC TTAGCAAGCT ACCTGAAATG CTGAAAATGT TCGAAGATCG TTTATGTCAT
  541 AAAACATATT TAAATGGTGA TCATGTAACC CATCCTGACT TCATGTTGTA TGACGCTCTT
  601 GATGTTGTTT TATACATGGA CCCAATGTGC CTGGATGCGT TCCCAAAATT AGTTTGTTTT
  661 AAAAAACGTA TTGAAGCTAT CCCACAAATT GATAAGTACT TGAAATCCAG CAAGTATATA
  721 GCATGGCCTT TGCAGGGCTG GCAAGCCACG TTTGGTGGTG GCGACCATCC TCCAAAATCG
  781 GATCTGGTTC CGCGTGGATC CATGAGCGCC CCGGGCAGCC CCGACCAGGC CTATGANTTC
  841 CTGCTCNAGT TCCTGCTGGT GGGCGANAGG GNNGTANGNN AGANTGAGAN CCTGGAGAGC
  901 CTGCNNGNNG GNGCANCTGA NTCCCCNTNC AGCCNTCTCN GGGGGNATCG ANNACNAGAN
  961 NANNACCATC CNGNTGGNNN NNNAGCGGGN GNNNNTNNAN NTCTGGGNNN NNNNNGGGNN
1021 NGNNNNNNTT TGTANNNNNN NNNN
//
Primer: SV40pA3element Reverse (Pr0108)
Region: lacI pro,lacI
Sequence file: RDB16862_A9A8d.seq check
>D06060D9_A9A8_2_SV40pA3'element Reverse_C01_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG CNNGCAACGC GGCCTTTTTA CGGTTCCTGG
   61 CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA
  121 ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA
  181 GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC
  241 TGTGCGGTAT TTCACACCGC ATAAATTCCG ACACCATCGA ATGGCGCAAA ACCTTTCGCG
  301 GTATGGCATG ATAGCGCCCG GAAGAGAGTC AATTCAGGGT GGTGAATGTG AAACCAGTAA
  361 CGTTATACGA TGTCGCAGAG TATGCCGGTG TCTCTTATCA GACCGTTTCC CGCGTGGTGA
  421 ACCAGGCCAG CCACGTTTCT GCGAAAACGC GGGAAAAAGT GGAAGCGGCG ATGGCGGAGC
  481 TGAATTACAT TCCCAACCGC GTGGCACAAC AACTGGCGGG CAAACAGTCG TTGCTGATTG
  541 GCGTTGCCAC CTCCAGTCTG GCCCTGCACG CGCCGTCGCA AATTGTCGCG GCGATTAAAT
  601 CTCGCGCCGA TCAACTGGGT GCCAGCGTGG TGGTGTCGAT GGTAGAACGA AGCGGCGTCG
  661 AAGCCTGTAA AGCGGCGGTG CACAATCTTC TCGCGCAACG CGTCAGTGGG CTGATCATTA
  721 ACTATCCGCT GGATGACCAG GATGCCATTG CTGTGGAAGC TGCCTGCACT AATGTTCCGG
  781 CGTTATTTCT TGATGTCTCT GANCANACAC CCATCAACAG TATTATTTTC TCCCATGAAG
  841 ACNGTACGCG ACTGGGCGTG NANCATCTGG TCGCNNTGGG TCACCAGCAA ATCGCGCTGT
  901 TANCGGGNCC NTTAAGTTCT GNCTCGGCGC GNCTGCGTCN GGCTNNNTGG NATNAATATC
  961 TCANNCGCNN NNAAATTCAG NCNNANANCG GNNNNGGANN NNNANNNNNN NGNCNNGNNC
1021 NNNTTTNNNN NNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Itoh, T., Screening for target Rabs of TBC (Tre-2/Bub2/Cdc16) domain-containing proteins based on their Rab-binding activity. Genes Cells 11 (9): 1023-1037 (2006). PMID 16923123. [link to RRC of NBRP]