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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGEX-4T-3-Rab13 (#RDB16838)

Expression vector of mouse Rab13 tagged with GST at N-terminus, tac promoter.

Clone info. Expression vector of mouse Rab13 tagged with GST at N-terminus, tac promoter.
Comment Expression was confirmed by the depositor with immunoblotting.
Vector backbone pGEX-4T-3 (plasmid)
Selectable markers Amp^r
Gene/insert name mouse Rab13 cDNA
Reference of insert sequence AB232608 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Fukuda, M. J. Biol. Chem. 278, 15373-15380, 2003); (Itoh, T. et al. Genes Cells 11, 1023-1037, 2006)
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Fukuda, M. J. Biol. Chem. 278, 15373-15380, 2003); (Itoh, T. et al. Genes Cells 11, 1023-1037, 2006)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16838 pGEX-4T-3-Rab13 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGEX-4T-3-Rab13 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16838).

Reference section:

Itoh, T., Satoh, M., Kanno, E., Fukuda, M., Screening for target Rabs of TBC (Tre-2/Bub2/Cdc16) domain-containing proteins based on their Rab-binding activity. Genes Cells 11 (9): 1023-1037 (2006). PMID 16923123. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16838_A9A8p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGEX-F2 (Pr0403)
Region: GST,thrombin site,insert
Sequence file: RDB16838_A9A8a.seq check
>16838_16838_A9A8_pGEX-F2_C01_07_ABI24.ab1
    1 NNNNNNNNNN NNNTNNNNNN NNNTATTGAA GCTNTCCNNC AAATTGATAA GTACTTGAAA
   61 TCCAGCAAGT ATATAGCATG GCCTTTGCAG GGCTGGCAAG CCACGTTTGG TGGTGGCGAC
  121 CATCCTCCAA AATCGGATCT GGTTCCGCGT GGATCCATGG CCAAAGCCTA CGACCACCTC
  181 TTCAAGTTGC TGCTCATCGG GGACTCGGGG GTGGGCAAGA CTTGTCTGAT CATTCGCTTT
  241 GCAGAGGACA ACTTCAACAG CACTTACATC TCTACCATCG GAATTGATTT CAAGATCCGA
  301 ACCGTGGACA TAGAGGGGAA GAGGATCAAA CTGCAAGTGT GGGACACGGC TGGCCAAGAA
  361 CGATTCAAGA CAATAACTAC CGCCTATTAC CGTGGAGCCA TGGGCATTAT CCTCGTATAT
  421 GACATCACAG ATGAGAAATC CTTCGAGAAT ATTCAGAACT GGATGAAAAG CATCAAAGAG
  481 AATGCCTCTG CGGGAGTGGA GCGCCTCCTG CTGGGAAACA AGTGTGACAT GGAGGCCAAG
  541 CGGCAGGTGC AGAGAGAGCA GGCGGAGAAG TTGGCTCGAG AGCACAGAAT CCGATTTTTT
  601 GAGACGAGTG CCAAATCCAG TGTGAATGTG GATGAGGCTT TCAGTTCCCT GGCCCGTGAC
  661 ATCTTGCTCA AGACAGGAGG CCGGAGATCG GGAACCAACA GTAAGCCCTC AAGCACTGGC
  721 CTGAAAACAT CTGACAAGAA GAACAACAAG TGCTTGTTAG GCTGAAATCA CTAGTGAATT
  781 CGCGGCCGCA TCGTGACTGA CTGACGATCT GCCTCGCGCG TTTCGGTGAN GACNGNGAAA
  841 ACCTCTGACA CATGCAGCTC CCGGAGANGG NCACAGCTNG TCTGTAAGCG GATGNCGGGA
  901 NCAGANAAGC CCGNCANGNN GCGNCAGCNG NNGTTGNCGG NNGNCNGGGC GCANCCNTNN
961 NCCANNCNCN TANCGATAGC NNANNGNATA NNTCNNGNNN NNNANNGNNN NNNNNNN
//
Primer: pGEX-ptac_up-F (Pr0455)
Region: tac pro,lacO,GST
Sequence file: RDB16838_A9A8b.seq check
>16838_16838_A9A8_pGEX-ptaq_up_F_E01_13_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNG NTCTGNNNNN NTTCTGAAAT GAGCTGTTGA CNATTAATCA
   61 TCGGCTCGTA TAATGTGTGG AATTGTGAGC GGATAACAAT TTCACACAGG AAACAGTATT
  121 CATGTCCCCT ATACTAGGTT ATTGGAAAAT TAAGGGCCTT GTGCAACCCA CTCGACTTCT
  181 TTTGGAATAT CTTGAAGAAA AATATGAAGA GCATTTGTAT GAGCGCGATG AAGGTGATAA
  241 ATGGCGAAAC AAAAAGTTTG AATTGGGTTT GGAGTTTCCC AATCTTCCTT ATTATATTGA
  301 TGGTGATGTT AAATTAACAC AGTCTATGGC CATCATACGT TATATAGCTG ACAAGCACAA
  361 CATGTTGGGT GGTTGTCCAA AAGAGCGTGC AGAGATTTCA ATGCTTGAAG GAGCGGTTTT
  421 GGATATTAGA TACGGTGTTT CGAGAATTGC ATATAGTAAA GACTTTGAAA CTCTCAAAGT
  481 TGATTTTCTT AGCAAGCTAC CTGAAATGCT GAAAATGTTC GAAGATCGTT TATGTCATAA
  541 AACATATTTA AATGGTGATC ATGTAACCCA TCCTGACTTC ATGTTGTATG ACGCTCTTGA
  601 TGTTGTTTTA TACATGGACC CAATGTGCCT GGATGCGTTC CCAAAATTAG TTTGTTTTAA
  661 AAAACGTATT GAAGCTATCC CACAAATTGA TAAGTACTTG AAATCCAGCA AGTATATAGC
  721 ATGGCCTTTG CAGGGCTGGC AAGCCACGTT TGGTGNTGGC GACCATCCTC CNAAATCGGA
  781 TCTGGTTCCG CGTGGATCCN TGGCCNAAGC CTACGACCAC CTCTTCNAGT TGCTGCTCAT
  841 CGGGGANTCN GGGGTGGGCA AGANTTGTCT GANCATTCGC TTTGCANANG ANAACNNCAN
  901 CNGCNCTTAC ATCTCTACNN TCNNANNTGA NTTCNNGATN CNAACCGNNN NNNTANAGGG
  961 GAAGANNNNC AAANNGCANN NGTGGNNNNN NGNNNGNNNN NNANNNANNC NNNNNNNNNN
//
Primer: pBAC_lac_F (Pr0544)
Region: lacI
Sequence file: RDB16838_A9A8c.seq check
>16838_16838_A9A8_pBAC_lac_F_F01_16_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNC CGNANNNTGG TCNTAGCTGT TTCCTGTNNT GAAATTGTTA
   61 TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC
  121 CTAATGAGTG AGCTAACTTA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG
  181 AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG
  241 TATTGGGCGC CAGGGTGGTT TTTCTTTTCA CCAGTGAGAC GGGCAACAGC TGATTGCCCT
  301 TCACCGCCTG GCCCTGAGAG AGTTGCAGCA AGCGGTCCAC GCTGGTTTGC CCCAGCAGGC
  361 GAAAATCCTG TTTGATGGTG GTTAACGGCG GGATATAACA TGAGCTGTCT TCGGTATCGT
  421 CGTATCCCAC TACCGAGATA TCCGCACCAA CGCGCAGCCC GGACTCGGTA ATGGCGCGCA
  481 TTGCGCCCAG CGCCATCTGA TCGTTGGCAA CCAGCATCGC AGTGGGAACG ATGCCCTCAT
  541 TCAGCATTTG CATGGTTTGT TGAAAACCGG ACATGGCACT CCAGTCGCCT TCCCGTTCCG
  601 CTATCGGCTG AATTTGATTG CGAGTGAGAT ATTTATGCCA GCCAGCCAGA CGCAGACGCG
  661 CCGAGACAGA ACTTAATGGG CCCGCTAACA GCGCGATTTG CTGGTGACCC AATGCGACCA
  721 GATGCTCCAC GCCCAGTCGC GTANCGTCTT CATGGGAGAA AATAATACTG TTGATGGGTG
  781 TCTGGTCAGA GACATCNAGA AATAACGCCN GANCATTAGT GCAGGCAGCT TCCACAGCAA
  841 TGGCATCCTG NTCATNCNGC GGATAGTTAA TGATCNGCCC ANNGACGCNT TGCGCGAGAA
  901 GANTGNGCAC CGCCGCTTTA CAGGCTTCNA NGNCGNTTCN TTCTACCNTC GANNNNNNNN
961 NN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Itoh, T., Screening for target Rabs of TBC (Tre-2/Bub2/Cdc16) domain-containing proteins based on their Rab-binding activity. Genes Cells 11 (9): 1023-1037 (2006). PMID 16923123. [link to RRC of NBRP]
reference Fukuda, M., Distinct Rab binding specificity of Rim1, Rim2, rabphilin, and Noc2. Identification of a critical determinant of Rab3A/Rab27A recognition by Rim2. J. Biol. Chem. 278 (17): 15373-15380 (2003). PMID 12578829. [link to RRC of NBRP]