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pGEX-4T-3-Rab10

Expression vector of mouse Rab10 tagged with GST at N-terminus, tac promoter.

Catalog number RDB16836
Resource name pGEX-4T-3-Rab10
Clone info. Expression vector of mouse Rab10 tagged with GST at N-terminus, tac promoter.
Expression was confirmed by the depositor with immunoblotting.
Vector backbone pGEX-4T-3 (plasmid)
Selectable markers Amp^r
Gene/insert name mouse Rab10 cDNA
Reference of nucleotide sequence AB232604 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
 
Other clones in our bank mouse Rab10 (NCBI Gene 19325) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kuroda, T. S. et al. J. Biol. Chem. 277, 9212-9218, 2002); (Itoh, T. et al. Genes Cells 11, 1023-1037, 2006)
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RDB16836 pGEX-4T-3-Rab10 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Itoh, T., Screening for target Rabs of TBC (Tre-2/Bub2/Cdc16) domain-containing proteins based on their Rab-binding activity. Genes Cells 11 (9): 1023-1037 (2006). PMID 16923123.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Rab family protein (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16836_A9A8p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGEX-F2
Region: GST 3', thrombin site, Insert 5'
Sequence file: RDB16836_A9A8a.seq check
>D06060B1_A9A8_3_pGEX-F2_A01_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNTNNNN NNNGTNNTNN NCTNTCCCNC AAATTGATAA GTACTTGAAA
   61 TCCAGCAAGT ATATAGCATG GCCTTTGCAG GGCTGGCAAG CCACGTTTGG TGGTGGCGAC
  121 CATCCTCCAA AATCGGATCT GGTTCCGCGT GGATCCATGG CGAAGAAGAC GTACGACCTG
  181 CTTTTCAAGC TGCTCCTGAT CGGGGACTCG GGAGTGGGCA AGACCTGCGT CCTTTTTCGT
  241 TTTTCGGACG ATGCCTTCAA TACCACCTTT ATTTCCACCA TAGGAATAGA CTTTAAGATC
  301 AAAACAGTGG AACTACAAGG AAAGAAGATC AAGCTACAGA TATGGGACAC AGCAGGCCAG
  361 GAGCGATTTC ACACCATCAC AACCTCCTAC TACAGAGGAG CAATGGGCAT CATGCTAGTG
  421 TATGACATCA CCAACGGTAA AAGCTTTGAG AACATCAGCA AGTGGCTTAG AAACATAGAT
  481 GAGCATGCCA ATGAAGATGT GGAAAGAATG TTACTAGGGA ACAAGTGTGA CATGGACGAC
  541 AAGAGAGTTG TACCGAAAGG CAAAGGAGAA CAGATTGCAA GGGAGCATGG TATTAGGTTT
  601 TTTGAGACTA GTGCAAAAGC AAATATAAAC ATCGAAAAGG CGTTCCTCAC ATTAGCTGAA
  661 GACATCCTCC GAAAGACCCC TGTAAAAGAA CCCAACAGTG AAAACGTAGA TATCAGCAGT
  721 GGANGANGCG TGACGGGCTG GAAGAGCAAG TGCTGCTGNA NTCACTAGTG AATTCGCGGC
  781 CGCATCGTGA CTGACTGACG ATCTGCCTCG CGCGNTTCGG TNNNGACNGT GAAAACNTCT
  841 GACACATGCA GCTNCNGGAN ANGGNCACAG CTNGNCTGTA AGCGGATGNC GGNNNCAGAN
  901 NNGCCCNNCA GGGNNNNNNA GCGGGNNNNG NCNGNNNNCN GGGGCNCNNN NNNNNNCNNN
961 NNNNNNN
//
Primer: pGEX3'
Region: Insert, thrombin site, GST 3'
Sequence file: RDB16836_A9A8b.seq check
>D06060B1_A9A8_3_pGEX3'_B01_02_ABI08.ab1
    1 NNNNNNNNNN NNNNCNNNNN NGNANNNCGT CNGTCNGTCN CGATGCGGCC GCGAATTCAC
   61 TAGTGATTTC AGCAGCACTT GCTCTTCCAG CCCGTCACGC CTCCTCCACT GCTGATATCT
  121 ACGTTTTCAC TGTTGGGTTC TTTTACAGGG GTCTTTCGGA GGATGTCTTC AGCTAATGTG
  181 AGGAACGCCT TTTCGATGTT TATATTTGCT TTTGCACTAG TCTCAAAAAA CCTAATACCA
  241 TGCTCCCTTG CAATCTGTTC TCCTTTGCCT TTCGGTACAA CTCTCTTGTC GTCCATGTCA
  301 CACTTGTTCC CTAGTAACAT TCTTTCCACA TCTTCATTGG CATGCTCATC TATGTTTCTA
  361 AGCCACTTGC TGATGTTCTC AAAGCTTTTA CCGTTGGTGA TGTCATACAC TAGCATGATG
  421 CCCATTGCTC CTCTGTAGTA GGAGGTTGTG ATGGTGTGAA ATCGCTCCTG GCCTGCTGTG
  481 TCCCATATCT GTAGCTTGAT CTTCTTTCCT TGTAGTTCCA CTGTTTTGAT CTTAAAGTCT
  541 ATTCCTATGG TGGAAATAAA GGTGGTATTG AAGGCATCGT CCGAAAAACG AAAAAGGACG
  601 CAGGTCTTGC CCACTCCCGA GTCCCCGATC AGGAGCAGCT TGAAAAGCAG GTCGTACGTC
  661 TTCTTCGCCA TGGATCCACG CGGAACCAGA TCCGATTTTG GAGGATGGTC GCCACCACCA
  721 AACGTGGCTT GCCAGCCCTG CAAAGGCCAT GCTATATACT TGCTGGANTT CAAGTACTTA
  781 TCAATTTGTG GGANAGCTTC AATACGTTTT TTAAAACAAA CTAATTTTGG GAACGCATCC
  841 AGGCACATTN GGTCCATGTA TAAAACAACA TCAAGANCGT CNTACAACAT GAANNCAGGA
  901 TGGGTTACNN GANCNNNNTT NNAANATGTT TTATGANNTA AACNATCTNC NNNNNTTTTN
  961 CAGCATTTCN NNTANCTTGN NNNNNAANNN NNNNTNNNNN NNTNNNNNNN NTNNNNNNNN
1021 NN
//
Primer: pGEX-ptac_up-F
Region: tac pro, tac op,GST
Sequence file: RDB16836_A9A8c.seq check
>D06060B1_A9A8_3_pGEX-ptac_up-F_C01_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN GTNCTGNNNN NNNTCTGNNN TGAGCTGTTG ANAATTAATC
   61 ATCGGCTCGT ATAATGTGTG GAATTGTGAG CGGATAACAA TTTCACACAG GAAACAGTAT
  121 TCATGTCCCC TATACTAGGT TATTGGAAAA TTAAGGGCCT TGTGCAACCC ACTCGACTTC
  181 TTTTGGAATA TCTTGAAGAA AAATATGAAG AGCATTTGTA TGAGCGCGAT GAAGGTGATA
  241 AATGGCGAAA CAAAAAGTTT GAATTGGGTT TGGAGTTTCC CAATCTTCCT TATTATATTG
  301 ATGGTGATGT TAAATTAACA CAGTCTATGG CCATCATACG TTATATAGCT GACAAGCACA
  361 ACATGTTGGG TGGTTGTCCA AAAGAGCGTG CAGAGATTTC AATGCTTGAA GGAGCGGTTT
  421 TGGATATTAG ATACGGTGTT TCGAGAATTG CATATAGTAA AGACTTTGAA ACTCTCAAAG
  481 TTGATTTTCT TAGCAAGCTA CCTGAAATGC TGAAAATGTT CGAAGATCGT TTATGTCATA
  541 AAACATATTT AAATGGTGAT CATGTAACCC ATCCTGACTT CATGTTGTAT GACGCTCTTG
  601 ATGTTGTTTT ATACATGGAC CCAATGTGCC TGGATGCGTT CCCAAAATTA GTTTGTTTTA
  661 AAAAACGTAT TGAAGCTATC CCACAAATTG ATAAGTACTT GAAATCCAGC AAGTATATAG
  721 CATGGCCTTT GCAGGGCTGG CAAGCCACGT TTGGTGGTGG CGACCATCCT CNNAAATCGG
  781 ATCTGGTTCC GCGTGGATCC ATGGCGAAGA ANACGTACGA CCTGCTTTTC AAGCTGCTCC
  841 TGATCGGGGN NTCGGGANTG GGCAAGANCT GCNNNCTTTT TTCGTTTTTC NGANGATGCC
  901 TNNNATANNA CCTTTNTTNC CNCCNTNNGA ATANNNTTTN AGNNCNAAAC ANNNNNANCT
961 NNNNNGNAAA NNNNNNNNNN
//
Primer: lacIpro-B_F
Region: lacI pro, lacI 5'
Sequence file: RDB16836_A9A8d.seq check
>D06060B1_A9A8_3_lacIpro-B_F_D01_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNCN NCGNNNCNGT GAGCGNNGAA NCGGAAGAGC GCCTGNTGCG
   61 GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATAAATTCCG ACACCATCGA
  121 ATGGCGCAAA ACCTTTCGCG GTATGGCATG ATAGCGCCCG GAAGAGAGTC AATTCAGGGT
  181 GGTGAATGTG AAACCAGTAA CGTTATACGA TGTCGCAGAG TATGCCGGTG TCTCTTATCA
  241 GACCGTTTCC CGCGTGGTGA ACCAGGCCAG CCACGTTTCT GCGAAAACGC GGGAAAAAGT
  301 GGAAGCGGCG ATGGCGGAGC TGAATTACAT TCCCAACCGC GTGGCACAAC AACTGGCGGG
  361 CAAACAGTCG TTGCTGATTG GCGTTGCCAC CTCCAGTCTG GCCCTGCACG CGCCGTCGCA
  421 AATTGTCGCG GCGATTAAAT CTCGCGCCGA TCAACTGGGT GCCAGCGTGG TGGTGTCGAT
  481 GGTAGAACGA AGCGGCGTCG AAGCCTGTAA AGCGGCGGTG CACAATCTTC TCGCGCAACG
  541 CGTCAGTGGG CTGATCATTA ACTATCCGCT GGATGACCAG GATGCCATTG CTGTGGAAGC
  601 TGCCTGCACT AATGTTCCGG CGTTATTTCT TGATGTCTCT GACCAGACAC CCATCAACAG
  661 TATTATTTTC TCCCATGAAG ACGGTACGCG ACTGGGCGTG GAGCATCTGG TCGCATTGGG
  721 TCACCAGCAA ATCGCGCTGT TAGCGGGCCC ATTAAGTTCT GTCTCGGCGC GTCTGCGTCT
  781 GGCTGGCTGG CATAAATATC TCACTCGCAA TCAAATTCAG CCGATAGCGG AACGGNNANG
  841 CGANTGNNNT GNCATGNCCG GTTTTCAACA AACCATGNAA ATGCTGAATG ANGGCATCGN
  901 TCCNACNNCN ATGCNGGTTN NCNACNATCN NATGGNGCTN GGNNCNNNGC NNGNNNNNAC
961 NNNNNNNNNG NNNNNNCNNT NNNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Itoh, T., Screening for target Rabs of TBC (Tre-2/Bub2/Cdc16) domain-containing proteins based on their Rab-binding activity. Genes Cells 11 (9): 1023-1037 (2006). PMID 16923123.
reference Kuroda, T.S., The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain. J. Biol. Chem., 277, 9212-9218 (2002). PMID 11773082.

2020.10.02

GNP_filter3_RDBDEP_html_200316.pl