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pCMV-ChRFR-Venus

Mammalian expression vector encoding a mammalian codon-optimized chopFR.

Catalog number RDB16773
Resource name pCMV-ChRFR-Venus
Clone info. Mammalian expression vector encoding a mammalian codon-optimized chopFR (channelopsin/fast-receiver) tagged with Venus at the C-terminus under CMV promoter.
Expression was confirmed by the depositor with whole-cell patch-clamp recording.
Vector backbone pVenus-N1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name Chlamydomonas reinhardtii acop1 (channelopsin-1) cDNA Chlamydomonas reinhardtii cop4 (acop2) cDNA
Depositor Ishizuka, Toru |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Wang, H. et al., J. Biol. Chem. 284 (9): 5685, 2009) For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
Additional terms and conditions for distribution Regarding the use of Venus, a written approval of Dr. Atsusi Miyawaki is required.
Ordering Please visit Information of Request for Distribution. 
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Material Transfer Agreement (MTA) [Word]
Approval form [Word] by Dr. Atsushi Miyawaki
Remarks
Please obtain written approval of Dr. Atsushi Miyawaki.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16773 pCMV-ChRFR-Venus DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content Genetic Resources for Optgenetics Research (English text)

References

original Wang, H., Molecular determinants differentiating photocurrent properties of two channelrhodopsins from chlamydomonas. J. Biol. Chem. 284 (9): 5685-5696 (2009). PMID 19103605.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB16773_A8L5p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB16773_A8L5a.seq
>D06115A5_A8L5_3_CMV_Forward_E03_15_ABI24.ab1
    1 CGTCAACTCG CCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
   61 GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA CCGGACTCAG ATCTCGAGCT
  121 CAAGCTTCGA ATTCCACCAT GGCGAGAAGA CCCTGGCTGC TGGCTCTGGC TCTGGCCGTC
  181 GCCCTGGCTG CCGGTTCCGC TGGTGCCTCA ACTGGCTCCG ATGCTACTGT CCCCGTGGCC
  241 ACACAGGACG GCCCTGATTA CGTCTTCCAC CGAGCTCATG AGAGGATGCT GTTTCAGACT
  301 TCCTATACCC TGGAAAACAA TGGGTCTGTG ATCTGCATTC CCAACAATGG CCAGTGCTTC
  361 TGTCTGGCAT GGCTCAAGTC TAACGGAACT AATGCCGAGA AACTGGCCGC TAACATCCTC
  421 CAGTGGATTA CCTTCGCCCT GAGCGCCCTG TGCCTCATGT TTTACGGCTA TCAGACATGG
  481 AAGTCCACTT GTGGATGGGA GGAAATCTAC GTGGCCACTA TCGAGATGAT CAAGTTCATC
  541 ATTGAATACT TCCACGAGTT TGACGAACCT GCAGTGATCT ATAGCTCCAA CGGCAATAAG
  601 ACAGTCTGGC TGAGATACGC TGAGTGGCTG CTCACTTGTC CAGTGATCCT GATTCATCTC
  661 AGCAACCTGA CAGGGCTCTC TAATGACTAT AGTAGGAGAA CTATGGGCCT GCTCGTGAGT
  721 GATATCGGGA CCATTGTCTG GGGTGCCACT TCAGCTATGG CAACCGGATA CGTGAAAGTC
  781 ATCTTCTTTT GCCTGGGGCT CTGTTATGGT GCTAACACAT TCTTTCACGC AGCCAAGGCA
  841 TACATCGAGG GCTATCATAC CGTGCCTAAA GGACGGTGCC GCCAGGTGGT CACAGGGATG
  901 GCTTGGCTGT TCTTTGTCTC TTGGGGGGAT GTTCCCAATC CTGTTTATTC TCGGTCCCGA
  961 AAGGGTTCGG TGTGCTGTCA GTCTACGGGC AGCACCGTGG GACACACAAA TCAATTGACC
 1021 TGATGAGTAA GAACTGTTTG GGGCCCTGCT CGGACACTAT CTGAGAGTGC TCAATCAACG
1081 GAAGCCATAT CATGAATTCC ATGGAGAAAA TATATTTTCG GGAAGGAAAC TCA
//
Primer: Venus-N_R RDB16773_A8L5b.seq
>D06115A5_A8L5_3_Venus-N_R_F03_18_ABI24.ab1
    1 TCGGTCGGAG CTGACTTGTG GCCGTTTACG TCGCCGTCCA GCTCGACCAG GATGGGCACC
   61 ACCCCGGTGA ACAGCTCCTC GCCCTTGCTC ACCATGGTGG CGACCGGTGG ATCCTTTCCA
  121 GTGCCTTTGT TGACTGCTCC AGCCTCAGCC TCATCCTCCA CGAGTGTTTC CACCTCAATT
  181 TCGGTTCCGC CGATATTCAG TTTTGTGGTC TTCCGAATAT CTCCATGAAT CAGGATATGC
  241 TCGTGGATGA GCACTCTCAG ATAGTGTCCG AGCAGGCCCC AACAGTTCTT ACTCATCAGG
  301 TCAATGATTG TGTGTCCCAC GGTGCTGCCG TAGACTGACA GCACACCGAA CCCTTCGGGA
  361 CCGAGAATAA ACAGGATTGG GAACATCCCC CAAGAGACAA AGAACAGCCA AGCCATCCCT
  421 GTGACCACCT GGCGGCACCG TCCTTTAGGC ACGGTATGAT AGCCCTCGAT GTATGCCTTG
  481 GCTGCGTGAA AGAATGTGTT AGCACCATAA CAGAGCCCCA GGCAAAAGAA GATGACTTTC
  541 ACGTATCCGG TTGCCATAGC TGAAGTGGCA CCCCAGACAA TGGTCCCGAT ATCACTCACG
  601 AGCAGGCCCA TAGTTCTCCT ACTATAGTCA TTAGAGAGCC CTGTCAGGTT GCTGAGATGA
  661 ATCAGGATCA CTGGACAAGT GAGCAGCCAC TCAGCGTATC TCAGCCAGAC TGTCTTATTG
  721 CCGTTGGAGC TATAGATCAC TGCAGGTTCG TCAAACTCGT GGAAGTATTC AATGATGAAC
  781 TTGATCATCT CGATAGTGGC CACGTAGATT TCCTCCCATC CACAAGTGGA CTTCCATGTC
  841 TGATAGCCGT AAAACATGAG GCACAGGGCG CTCAGGGCGA AGGTAATCCA CTGGAGGATG
  901 TTAGCGGCCA GTTTCTCGGC ATTAGTTCCG TTAGACTTGA GCCATGCCAG ACAGAAGCAC
  961 TGGCCCATTG TTTGGGAATG CAGATCACAG ACCATTGTTT TTCCCAGGGT ATAGGAAAGT
 1021 CTGAAACCAG CCATCTCCTC ATGAGCTTCG ATGAGACGTT ATTCAGGTCC GTCCTGTGTG
1081 CACAGGACAG GTAGCAATCG GAGTCCAAGT
//
Primer: SV40polyA-R RDB16773_A8L5c.seq
>D06115A5_A8L5_3_SV40polyA-R_G03_21_ABI24.ab1
    1 AAAAATGGCA GTTTTAAGCA GTAAAACCTC TACAAATGTG GTATGGCTGA TTATGATCTA
   61 GAGTCGCGGC CGCTTACTTG TACAGCTCGT CCATGCCGAG AGTGATCCCG GCGGCGGTCA
  121 CGAACTCCAG CAGGACCATG TGATCGCGCT TCTCGTTGGG GTCTTTGCTC AGGGCGGACT
  181 GGTAGCTCAG GTAGTGGTTG TCGGGCAGCA GCACGGGGCC GTCGCCGATG GGGGTGTTCT
  241 GCTGGTAGTG GTCGGCGAGC TGCACGCCGC CGTCCTCGAT GTTGTGGCGG ATCTTGAAGT
  301 TGGCCTTGAT GCCGTTCTTC TGCTTGTCGG CGGTGATATA GACGTTGTGG CTGTTGTAGT
  361 TGTACTCCAG CTTGTGCCCC AGGATGTTGC CGTCCTCCTT GAAGTCGATG CCCTTCAGCT
  421 CGATGCGGTT CACCAGGGTG TCGCCCTCGA ACTTCACCTC GGCGCGGGTC TTGTAGTTGC
  481 CGTCGTCCTT GAAGAAGATG GTGCGCTCCT GGACGTAGCC TTCGGGCATG GCGGACTTGA
  541 AGAAGTCGTG CTGCTTCATG TGGTCGGGGT AGCGGGCGAA GCACTGCAGG CCGTAGCCCA
  601 GGGTGGTCAC GAGGGTGGGC CAGGGCACGG GCAGCTTGCC GGTGGTGCAG ATCAGCTTCA
  661 GGGTCAGCTT GCCGTAGGTG GCATCGCCCT CGCCCTCGCC GGACACGCTG AACTTGTGGC
  721 CGTTTACGTC GCCGTCCAGC TCGACCAGGA TGGGCACCAC CCCGGTGAAC AGCTCCTCGC
  781 CCTTGCTCAC CATGGTGGCG ACCGGTGGAT CCTTTCCAGT GCCTTTGTTG ACTGCTCCAG
  841 CCTCAGCCTC ATCCTCCACG AGTGTTTCCA CCTCAATTTC GGTTCCGCCG ATATTCAGTT
  901 TTGTGGTCTT CCGAATATCT CCATGAATCA CGATATGCTC GTGGATGAGC ACTCTCAGAT
  961 AGTGTCCGAG CAGGCCCCAA CAGTTCTTAC TCATCAGGTC AATGATTGTG TGTCCTCACG
 1021 GGTGCTGCTC GTAGACTGAC AGCACCACCC GAACTCCTTC GGGGAACCGA AGAATAAACA
 1081 GGATTTGGGC AACCATTCCC CTCGAGAGAA CAAAGGATCA GCCTAAGCTC ATCCCCTGGT
1141 GAAACTAAT
//
Primer: SV40pro_F RDB16773_A8L5a.seqd.seq
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Wang, H., Molecular determinants differentiating photocurrent properties of two channelrhodopsins from chlamydomonas. J. Biol. Chem. 284 (9): 5685-5696 (2009). PMID 19103605.

2019.03.08

GNP_filter3_RDBDEP_html_190307.pl