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pCMV-ChRFR-Venus

Mammalian expression vector encoding a mammalian codon-optimized chopFR.

Catalog number RDB16773
Resource name pCMV-ChRFR-Venus
Clone info. Mammalian expression vector encoding a mammalian codon-optimized chopFR (channelopsin/fast-receiver) tagged with Venus at the C-terminus under CMV promoter.
Comment Expression was confirmed by the depositor with whole-cell patch-clamp recording.
Vector backbone pVenus-N1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name Chlamydomonas reinhardtii acop1 (channelopsin-1) cDNA Chlamydomonas reinhardtii cop4 (acop2) cDNA
Depositor|Developer Ishizuka, Toru |
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External Database
Chlamydomonas reinhardtii acop1 (channelopsin-1) Chlamydomonas reinhardtii cop4 (acop2)

        Reference sequence
          
         

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Wang, H. et al., J. Biol. Chem. 284 (9): 5685, 2009) For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
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        提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Wang, H. et al., J. Biol. Chem. 284 (9): 5685, 2009)。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。
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        RDB16773 pCMV-ChRFR-Venus DNA solution

        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        How to cite this biological resource

        Materials & Methods section:

        The pCMV-ChRFR-Venus was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16773).

        Reference section:

        Wang, H., Sugiyama, Y., Hikima, T., Sugano, E., Tomita, H., Takahashi, T., Ishizuka, T., Yawo, H., Molecular determinants differentiating photocurrent properties of two channelrhodopsins from chlamydomonas. J. Biol. Chem. 284 (9): 5685-5696 (2009). PMID 19103605. [link to RRC of NBRP]

        Further references such as user reports and related articles (go to bottom)


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        Featured content Genetic Resources for Optgenetics Research (English text)

        QC test results

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB16773_A8L5p1.pdf check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: CMV_Forward ()
        Sequence file: RDB16773_A8L5a.seq check
        >D06115A5_A8L5_3_CMV_Forward_E03_15_ABI24.ab1
            1 CGTCAACTCG CCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
           61 GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA CCGGACTCAG ATCTCGAGCT
          121 CAAGCTTCGA ATTCCACCAT GGCGAGAAGA CCCTGGCTGC TGGCTCTGGC TCTGGCCGTC
          181 GCCCTGGCTG CCGGTTCCGC TGGTGCCTCA ACTGGCTCCG ATGCTACTGT CCCCGTGGCC
          241 ACACAGGACG GCCCTGATTA CGTCTTCCAC CGAGCTCATG AGAGGATGCT GTTTCAGACT
          301 TCCTATACCC TGGAAAACAA TGGGTCTGTG ATCTGCATTC CCAACAATGG CCAGTGCTTC
          361 TGTCTGGCAT GGCTCAAGTC TAACGGAACT AATGCCGAGA AACTGGCCGC TAACATCCTC
          421 CAGTGGATTA CCTTCGCCCT GAGCGCCCTG TGCCTCATGT TTTACGGCTA TCAGACATGG
          481 AAGTCCACTT GTGGATGGGA GGAAATCTAC GTGGCCACTA TCGAGATGAT CAAGTTCATC
          541 ATTGAATACT TCCACGAGTT TGACGAACCT GCAGTGATCT ATAGCTCCAA CGGCAATAAG
          601 ACAGTCTGGC TGAGATACGC TGAGTGGCTG CTCACTTGTC CAGTGATCCT GATTCATCTC
          661 AGCAACCTGA CAGGGCTCTC TAATGACTAT AGTAGGAGAA CTATGGGCCT GCTCGTGAGT
          721 GATATCGGGA CCATTGTCTG GGGTGCCACT TCAGCTATGG CAACCGGATA CGTGAAAGTC
          781 ATCTTCTTTT GCCTGGGGCT CTGTTATGGT GCTAACACAT TCTTTCACGC AGCCAAGGCA
          841 TACATCGAGG GCTATCATAC CGTGCCTAAA GGACGGTGCC GCCAGGTGGT CACAGGGATG
          901 GCTTGGCTGT TCTTTGTCTC TTGGGGGGAT GTTCCCAATC CTGTTTATTC TCGGTCCCGA
          961 AAGGGTTCGG TGTGCTGTCA GTCTACGGGC AGCACCGTGG GACACACAAA TCAATTGACC
         1021 TGATGAGTAA GAACTGTTTG GGGCCCTGCT CGGACACTAT CTGAGAGTGC TCAATCAACG
        1081 GAAGCCATAT CATGAATTCC ATGGAGAAAA TATATTTTCG GGAAGGAAAC TCA
        //
        Primer: _Venus-N_R ()
        Sequence file: RDB16773_A8L5b.seq check
        >D06115A5_A8L5_3_Venus-N_R_F03_18_ABI24.ab1
            1 TCGGTCGGAG CTGACTTGTG GCCGTTTACG TCGCCGTCCA GCTCGACCAG GATGGGCACC
           61 ACCCCGGTGA ACAGCTCCTC GCCCTTGCTC ACCATGGTGG CGACCGGTGG ATCCTTTCCA
          121 GTGCCTTTGT TGACTGCTCC AGCCTCAGCC TCATCCTCCA CGAGTGTTTC CACCTCAATT
          181 TCGGTTCCGC CGATATTCAG TTTTGTGGTC TTCCGAATAT CTCCATGAAT CAGGATATGC
          241 TCGTGGATGA GCACTCTCAG ATAGTGTCCG AGCAGGCCCC AACAGTTCTT ACTCATCAGG
          301 TCAATGATTG TGTGTCCCAC GGTGCTGCCG TAGACTGACA GCACACCGAA CCCTTCGGGA
          361 CCGAGAATAA ACAGGATTGG GAACATCCCC CAAGAGACAA AGAACAGCCA AGCCATCCCT
          421 GTGACCACCT GGCGGCACCG TCCTTTAGGC ACGGTATGAT AGCCCTCGAT GTATGCCTTG
          481 GCTGCGTGAA AGAATGTGTT AGCACCATAA CAGAGCCCCA GGCAAAAGAA GATGACTTTC
          541 ACGTATCCGG TTGCCATAGC TGAAGTGGCA CCCCAGACAA TGGTCCCGAT ATCACTCACG
          601 AGCAGGCCCA TAGTTCTCCT ACTATAGTCA TTAGAGAGCC CTGTCAGGTT GCTGAGATGA
          661 ATCAGGATCA CTGGACAAGT GAGCAGCCAC TCAGCGTATC TCAGCCAGAC TGTCTTATTG
          721 CCGTTGGAGC TATAGATCAC TGCAGGTTCG TCAAACTCGT GGAAGTATTC AATGATGAAC
          781 TTGATCATCT CGATAGTGGC CACGTAGATT TCCTCCCATC CACAAGTGGA CTTCCATGTC
          841 TGATAGCCGT AAAACATGAG GCACAGGGCG CTCAGGGCGA AGGTAATCCA CTGGAGGATG
          901 TTAGCGGCCA GTTTCTCGGC ATTAGTTCCG TTAGACTTGA GCCATGCCAG ACAGAAGCAC
          961 TGGCCCATTG TTTGGGAATG CAGATCACAG ACCATTGTTT TTCCCAGGGT ATAGGAAAGT
         1021 CTGAAACCAG CCATCTCCTC ATGAGCTTCG ATGAGACGTT ATTCAGGTCC GTCCTGTGTG
        1081 CACAGGACAG GTAGCAATCG GAGTCCAAGT
        //
        Primer: SV40polyA-R ()
        Sequence file: RDB16773_A8L5c.seq check
        >D06115A5_A8L5_3_SV40polyA-R_G03_21_ABI24.ab1
            1 AAAAATGGCA GTTTTAAGCA GTAAAACCTC TACAAATGTG GTATGGCTGA TTATGATCTA
           61 GAGTCGCGGC CGCTTACTTG TACAGCTCGT CCATGCCGAG AGTGATCCCG GCGGCGGTCA
          121 CGAACTCCAG CAGGACCATG TGATCGCGCT TCTCGTTGGG GTCTTTGCTC AGGGCGGACT
          181 GGTAGCTCAG GTAGTGGTTG TCGGGCAGCA GCACGGGGCC GTCGCCGATG GGGGTGTTCT
          241 GCTGGTAGTG GTCGGCGAGC TGCACGCCGC CGTCCTCGAT GTTGTGGCGG ATCTTGAAGT
          301 TGGCCTTGAT GCCGTTCTTC TGCTTGTCGG CGGTGATATA GACGTTGTGG CTGTTGTAGT
          361 TGTACTCCAG CTTGTGCCCC AGGATGTTGC CGTCCTCCTT GAAGTCGATG CCCTTCAGCT
          421 CGATGCGGTT CACCAGGGTG TCGCCCTCGA ACTTCACCTC GGCGCGGGTC TTGTAGTTGC
          481 CGTCGTCCTT GAAGAAGATG GTGCGCTCCT GGACGTAGCC TTCGGGCATG GCGGACTTGA
          541 AGAAGTCGTG CTGCTTCATG TGGTCGGGGT AGCGGGCGAA GCACTGCAGG CCGTAGCCCA
          601 GGGTGGTCAC GAGGGTGGGC CAGGGCACGG GCAGCTTGCC GGTGGTGCAG ATCAGCTTCA
          661 GGGTCAGCTT GCCGTAGGTG GCATCGCCCT CGCCCTCGCC GGACACGCTG AACTTGTGGC
          721 CGTTTACGTC GCCGTCCAGC TCGACCAGGA TGGGCACCAC CCCGGTGAAC AGCTCCTCGC
          781 CCTTGCTCAC CATGGTGGCG ACCGGTGGAT CCTTTCCAGT GCCTTTGTTG ACTGCTCCAG
          841 CCTCAGCCTC ATCCTCCACG AGTGTTTCCA CCTCAATTTC GGTTCCGCCG ATATTCAGTT
          901 TTGTGGTCTT CCGAATATCT CCATGAATCA CGATATGCTC GTGGATGAGC ACTCTCAGAT
          961 AGTGTCCGAG CAGGCCCCAA CAGTTCTTAC TCATCAGGTC AATGATTGTG TGTCCTCACG
         1021 GGTGCTGCTC GTAGACTGAC AGCACCACCC GAACTCCTTC GGGGAACCGA AGAATAAACA
         1081 GGATTTGGGC AACCATTCCC CTCGAGAGAA CAAAGGATCA GCCTAAGCTC ATCCCCTGGT
        1141 GAAACTAAT
        //
        Primer: SV40pro_F
        Sequence file: RDB16773_A8L5a.seqd.seq check
        1

        Please visit Sequencing and PCR primers for primer information.


        References

        Original, user report and related articles

        original Wang, H., Molecular determinants differentiating photocurrent properties of two channelrhodopsins from chlamydomonas. J. Biol. Chem. 284 (9): 5685-5696 (2009). PMID 19103605. [link to RRC of NBRP]

        2023.11.23

        GNP_filter3_RDBDEP_html_231027.pl