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GFP-OPTN-WT (#RDB16707)

Expression vector of human optineurin (OPTN).

Clone info. Expression vector of human optineurin (OPTN), wild type, fused with enhanced monomeric EGFP at N-terminal, CMV promoter.
Comment Expression was confirmed by the deposior with fluorescence image.
Vector backbone pmEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cell).
Gene/insert name human OPTN cDNA
Depositor|Developer Kinjo, Masataka |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kitamura, A. et al., J. Biochem. 164 (3): 223-229, 2018)
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kitamura, A. et al., J. Biochem. 164 (3): 223-229, 2018)。

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RDB16707 GFP-OPTN-WT DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The GFP-OPTN-WT was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16707).

Reference section:

Kitamura, A., Shimizu, H., Kinjo, M., Determination of cytoplasmic optineurin foci sizes using image correlation spectroscopy. J. Biochem. 164 (3): 223-229 (2018). PMID 29684153. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16707_A8Ilp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: Venus-C_F2 (Pr0454)
Region: insert 5'
Sequence file: RDB16707_A8Ild.seq check
>D05635A1_A8Il__3_VenusC_F2_A01_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNCTC GNNNNGNNNG AGCTGTACAA GTCCGGACTC
   61 NNGATCTCGA GCTCAAGCTT CGAATTCTAT GTCCCATCAA CCTCTCAGCT GCCTCACTGA
  121 AAAGGAGGAC AGCCCCAGTG AAAGCACAGG AAATGGACCC CCCCACCTGG CCCACCCAAA
  181 CCTGGACACG TTTACCCCGG AGGAGCTGCT GCAGCAGATG AAAGAGCTCC TGACCGAGAA
  241 CCACCAGCTG AAAGAAGCCA TGAAGCTAAA TAATCAAGCC ATGAAAGGGA GATTTGAGGA
  301 GCTTTCGGCC TGGACAGAGA AACAGAAGGA AGAACGCCAG TTTTTTGAGA TACAGAGCAA
  361 AGAAGCAAAA GAGCGTCTAA TGGCCTTGAG TCATGAGAAT GAGAAATTGA AGGAAGAGCT
  421 TGGAAAACTA AAAGGGAAAT CAGAAAGGTC ATCTGAGGAC CCCACTGATG ACTCCAGGCT
  481 TCCCAGGGCC GAAGCGGAGC AGGAAAAGGA CCAGCTCAGG ACCCAGGTGG TGAGGCTACA
  541 AGCAGAGAAG GCAGACCTGT TGGGCATCGT GTCTGAACTG CAGCTCAAGC TGAACTCCAG
  601 CGGCTCCTCA GAAGATTCCT TTGTTGAAAT TAGGATGGCT GAAGGAGAAG CAGAAGGGTC
  661 AGTAAAAGAA ATCAAGCATA GTCCTGGGCC CACGAGAACA GTCTCCACTG GCACGGCATT
  721 GTCTAAATAT AGGAGCAGAT CTGCAGATGG GGCCAAGAAT TACTTCGAAC ATGANGAGTT
  781 AACTGTGAGC CAGCTCCTGC TGTGCCTAAG GGAANGGAAT CAGAAGGTGG AGAGACTTNA
  841 AGTTGCACTC ANGGNNGCCA AAGAAAGAGT TTCANATTTT GAAAAGAAAN NNAGTAATCG
  901 TTCTGNNATT GAAANCCNGA CAGAGGGGAN CACANAGAAA GANNNNGNTG AAGANAANNN
961 CCNNNNNANN GNNNGNANNC NNNNNNNANN CNCTNNNNCN NCCNNGNGNN NNNNNNNN
//
Primer: SV40polyA-R (Pr0109)
Region: insert 3'
Sequence file: RDB16707_A8Ile.seq check
>D05635A1-3_A8Il_SV40polyA-R_B01_04_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNGTAAA ACCTCNACNA ATGTGGTATG GCTGATTATG
   61 ATCAGTTATC TAGATCCGGT GGATCCCGAA TGATGCAATC CATCACGTGA ATCTGTAACG
  121 TGTCTATGTC AGGCAGAACC TCTCCACACT TGGGGCAGGA ATGAATCGGA ATATTCCGCT
  181 GTTGCCGCCA GTCCCTGTCC TCAGCTCCTC TTTGAACAAG GTAAGCCTGC TGGTCAGAGT
  241 CACTTGTTCT CGCCCCATGA CGACTCTGCA TCTCCATCAA GGACTGCCTG CCTCCGTCTT
  301 CGAAAGCATC ATTCTCTTTC AGCAGAACTG CCAGCTGCAA TGCCAGTTGC TCCTTTTCCT
  361 CATGAATTTT CTCTCTCGCT GCTCTTTCAG CATGAAAATC AGAACAGTAA ACTTCCATCT
  421 GAGCCCTGAG GATGGTCATG GTTTCCAGGT CCTCTTCCTG CTTGGCAATG GTTTGCTTCA
  481 TTTCATCCAT TTGCAGCTGT TTGGAAGCCA GAGCCTTCTC TGCCAGTTCC AGTTTTTCAC
  541 TCAGTTCCTT CAGCACTGCC CTGTCCACTT TTTCTGACTC TTTTCTTGTT AGTTCCTCAA
  601 TTGTTTTCAA TGCATTATTA TGTTCTTGAA GAAGCTTGTT GTGTGTCATC TGTAGCACAG
  661 TTAATTTGGA CTTTTCATCC TCTGTTTTAG CCTGTTCCAT TTTGATTTCT GATAGCATGC
  721 TTTCCACTTG TAGCTCTAAC TTTTTGTTAG TATAAACAAG CTCTTGCTTT TCATTCAACT
  781 CTGATGGAAT TGCAGANTTT TTCCTTTCNA GGGCCTGACA CTTTTCTTGN AAGTCTCTTC
  841 TTCATTAGCT CANCTTCGCT GANTTTTGTA TGANCCTCTT GAAGCTCCTT AAACANAGAT
  901 GTCACCTGGA NGNNCANTGC TNNCACTTCG CTTNCNACAG TCTCCNGGCC TTTNNCTTNN
  961 NCATTCNCNT TNNNNGNGCT NCCCNNCNGN CNGGNTTTNN ANCTNNNANN NNNNTNNNTN
1021 NTTTTNNTNN NNNANTCNGN AANNNNNTNN NTNNGNCNNN NNNNNNN
//
Primer: CMV_Forward (Pr0016)
Region: CMV pro,mEGFP 5'
Sequence file: RDB16707_A8Ilf.seq check
>16707_16707_B3B6F_CMV-Forward_E03_15_ABI24.ab1
    1 NNNNNNNNNN NNNTTGNNGC NNNGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC
   61 AGAGCTGGTT TAGTGAACCG TCAGATCCGC TAGCGCTACC GGTCGCCACC ATGGTGAGCA
  121 AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA
  181 ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA
  241 CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA
  301 CCCTGACCTA CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT
  361 TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG
  421 ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA
  481 TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT
  541 ACAACTACAA CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC GGCATCAAGG
  601 TGAACTTCAA GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC
  661 AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCA
  721 CCCAGTCCAA GCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT
  781 TCGTGACCGC CGCCNGGNTC ACTCTCGGCA TGGACGANCT GTACNAGTCC GGACTCAGAT
  841 CTCNAGCTCN AGCTTCNAAN TCTATGTCCN NTCAACCTCT CAGCTGCCTC ACTNNAAAGG
  901 ANGANNGCCC NNNNNAANGC ACNNNAAATG NNNCCCCCCN ANNNNNNCNN CCCAAANCNN
961 NNNNNNNN
//
Primer: SV40pro_F_V2 (Pr0154)
Region: NeoR_KanR
Sequence file: RDB16707_A8Ilg.seq check
>D05635A1-3_A8Il_SV40pro_F_V2_C01_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNGNNNC NNGGCTTTTG CNAAGANCGA TCNAGAGACA GGATGAGGAT
   61 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA
  121 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC
  181 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA
  241 ATGAACTGCA AGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG
  301 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC
  361 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG
  421 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA
  481 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC
  541 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGAGCA
  601 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG
  661 TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT
  721 ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG
  781 ANCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC
  841 GCCTTCTTGA CGAGTTCTTC TGANCGGGAN TCTGGGGTTC GAAATGANCG ANCAAGCGAN
  901 GCCCNACCTG CCNTCACGAN ATTTCGATTC CACCGNCGCC TTCNATGAAN NNNNGGGCTN
961 NNNNNNCGTT TTNNNGGNNN NNNGNNNNNN GANNNNNNNN CGNNGGGNTN NNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kitamura, A., Determination of cytoplasmic optineurin foci sizes using image correlation spectroscopy. J. Biochem. 164 (3): 223-229 (2018). PMID 29684153. [link to RRC of NBRP]
protocol Kitamura, A., Spatial Image Correlation Spectroscopy (ICS): A Technique for Average Size Determination of Subcellular Accumulated Structures from Fluorescence Microscopic Images. Bio-protocol 10(10): e3624 (2020). PMID 33659297. [link to RRC of NBRP]