RDB16707 - GFP-OPTN-WT

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kitamura, A. et al., J. Biochem. 164 (3): 223-229, 2018)
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kitamura, A. et al., J. Biochem. 164 (3): 223-229, 2018)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします（Schedule AとSchedule Bを入手[link])。

提供条件

利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kitamura, A. et al., J. Biochem. 164 (3): 223-229, 2018)。

提供依頼

手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
提供依頼書 [Word

]
GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします（Schedule AとSchedule Bを入手[link])。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB16707	GFP-OPTN-WT	DNA solution

original	Kitamura, A., Determination of cytoplasmic optineurin foci sizes using image correlation spectroscopy. J. Biochem. 164 (3): 223-229 (2018). PMID 29684153.

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GFP-OPTN-WT

Distribution information

References and tips

Sequence information

References

original	Kitamura, A., Determination of cytoplasmic optineurin foci sizes using image correlation spectroscopy. J. Biochem. 164 (3): 223-229 (2018). PMID 29684153.
protocol	Kitamura, A., Spatial Image Correlation Spectroscopy (ICS): A Technique for Average Size Determination of Subcellular Accumulated Structures from Fluorescence Microscopic Images. Bio-protocol 10(10): e3624 (2020). PMID 33659297.

Primer: VenusC_F2 Sequence file: RDB16707_A8Ila.seq
>D05635A1_A8Il__3_VenusC_F2_A01_01_ABI08.ab1 1 AAGCAGGGGA AAGCGGCCGG GATCACTCTC GGCATGGACG AGCTGTACAA GTCCGGACTC 61 AGATCTCGAG CTCAAGCTTC GAATTCTATG TCCCATCAAC CTCTCAGCTG CCTCACTGAA 121 AAGGAGGACA GCCCCAGTGA AAGCACAGGA AATGGACCCC CCCACCTGGC CCACCCAAAC 181 CTGGACACGT TTACCCCGGA GGAGCTGCTG CAGCAGATGA AAGAGCTCCT GACCGAGAAC 241 CACCAGCTGA AAGAAGCCAT GAAGCTAAAT AATCAAGCCA TGAAAGGGAG ATTTGAGGAG 301 CTTTCGGCCT GGACAGAGAA ACAGAAGGAA GAACGCCAGT TTTTTGAGAT ACAGAGCAAA 361 GAAGCAAAAG AGCGTCTAAT GGCCTTGAGT CATGAGAATG AGAAATTGAA GGAAGAGCTT 421 GGAAAACTAA AAGGGAAATC AGAAAGGTCA TCTGAGGACC CCACTGATGA CTCCAGGCTT 481 CCCAGGGCCG AAGCGGAGCA GGAAAAGGAC CAGCTCAGGA CCCAGGTGGT GAGGCTACAA 541 GCAGAGAAGG CAGACCTGTT GGGCATCGTG TCTGAACTGC AGCTCAAGCT GAACTCCAGC 601 GGCTCCTCAG AAGATTCCTT TGTTGAAATT AGGATGGCTG AAGGAGAAGC AGAAGGGTCA 661 GTAAAAGAAA TCAAGCATAG TCCTGGGCCC ACGAGAACAG TCTCCACTGG CACGGCATTG 721 TCTAAATATA GGAGCAGATC TGCAGATGGG GCCAAGAATT ACTTCGAACA TGAGGAGTTA 781 ACTGTGAGCC AGCTCCTGCT GTGCCTAAGG GAAGGGAATC AGAAGGTGGA GAGACTTGAA 841 GTTGCACTCA AGGAGGCCAA AGAAAGAGTT TCAGATTTTG AAAAGAAAAC AAGTAATCGT 901 TCTGAGATTG AAACCCAGAC AGAGGGGAGC ACAGAGAAAG AGAATGATGA AGAGAAAGGC 961 CCGGAGACTG TTGGAAAGCG AAGTGGAAGC ACTGAACCCT CCAGGTGACA TCTCTGTTTA 1021 AGGAGCTTCA AGGAGGCTCA ATACAAACTC AGCGAAGCTG AAGCTATGAG AGGAGACTTC 1081 AAGAAAGTGT CAGGCTGTTG AAGAAACTTC TGCAATTTCC CAA //
Primer: SV40polyA-R Sequence file: RDB16707_A8Ilb.seq
> GAAAGGGAGGTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTATGGCTGATTATGATCAGTTATCTAGATCCGGTGGAT 1 CCCGAATGAT GCAATCCATC ACGTGAATCT GTAACGTGTC TATGTCAGGC AGAACCTCTC 61 CACACTTGGG GCAGGAATGA ATCGGAATAT TCCGCTGTTG CCGCCAGTCC CTGTCCTCAG 121 CTCCTCTTTG AACAAGGTAA GCCTGCTGGT CAGAGTCACT TGTTCTCGCC CCATGACGAC 181 TCTGCATCTC CATCAAGGAC TGCCTGCCTC CGTCTTCGAA AGCATCATTC TCTTTCAGCA 241 GAACTGCCAG CTGCAATGCC AGTTGCTCCT TTTCCTCATG AATTTTCTCT CTCGCTGCTC 301 TTTCAGCATG AAAATCAGAA CAGTAAACTT CCATCTGAGC CCTGAGGATG GTCATGGTTT 361 CCAGGTCCTC TTCCTGCTTG GCAATGGTTT GCTTCATTTC ATCCATTTGC AGCTGTTTGG 421 AAGCCAGAGC CTTCTCTGCC AGTTCCAGTT TTTCACTCAG TTCCTTCAGC ACTGCCCTGT 481 CCACTTTTTC TGACTCTTTT CTTGTTAGTT CCTCAATTGT TTTCAATGCA TTATTATGTT 541 CTTGAAGAAG CTTGTTGTGT GTCATCTGTA GCACAGTTAA TTTGGACTTT TCATCCTCTG 601 TTTTAGCCTG TTCCATTTTG ATTTCTGATA GCATGCTTTC CACTTGTAGC TCTAACTTTT 661 TGTTAGTATA AACAAGCTCT TGCTTTTCAT TCAACTCTGA TGGAATTGCA GAATTTTTCC 721 TTTCAAGGGC CTGACACTTT TCTTGAAGTC TCTTCTTCAT TAGCTCAGCT TCGCTGAGTT 781 TTGTATGAGC CTCTTGAAGC TCCTTAAACA GAGATGTCAC CTGGAGGTTC AGTGCTTCCA 841 CTTCGCTTCC AACAGTCTCC GGGCCTTTCT CTTCATCATT CTCTTTCTCT GTGCTCCCTC 901 TGTCTGGGTT TCAATCTCAG AACGRATTAC TTGGTTTTCT TTTCAAAATC TGAAAACTCT 961 TTCTTTGGCC TCCTTGARTG CAACTTCAAG TCTCTCCACC TTCTGGATCC TCCTAGCACA 1021 GCAGGAGCTG GCTCACARGT TACTCCTCAT GTTCGAAGTA ATTCTTGGCA CAATCYGTGC 1081 MGAAAATCTK GCC //
Primer: SV40pro_F_V2 Sequence file: RDB16707_A8Ilc.seq
>D05635A1-3_A8Il_SV40pro_F_V2_C01_07_ABI24.ab1 1 ATAAGAGCTT TTTTGGAGCC TAGGCTTTTG CAAAGATCGA TCAAGAGACA GGATGAGGAT 61 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA 121 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC 181 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA 241 ATGAACTGCA AGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG 301 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC 361 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG 421 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA 481 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC 541 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGAGCA 601 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG 661 TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT 721 ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG 781 ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC 841 GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGTTC GAAATGACCG ACCAAGCGAC 901 GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT 961 CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA 1021 GTTCTTCGCC CACCCTAGGG GAGGCTAACT GAAAACACGG AGGAGACATA CCGGGAGGAC 1081 CCGCGCTATG ACGGCAATAA AAAAGACGGA TAAACGGCAC GGTGTGGGTC GGTTGTCATA 1141 AACGCGGGGA TTCGGGTTC //