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GFP-OPTN-WT

Expression vector of human optineurin (OPTN).

Catalog number RDB16707
Resource name GFP-OPTN-WT
Clone info. Expression vector of human optineurin (OPTN), wild type, fused with enhanced monomeric EGFP at N-terminal, CMV promoter.
Comment Expression was confirmed by the deposior with fluorescence image.
Vector backbone pmEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cell).
Gene/insert name human OPTN cDNA
Depositor|Developer Kinjo, Masataka |
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External Database
human OPTN

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kitamura, A. et al., J. Biochem. 164 (3): 223-229, 2018)
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kitamura, A. et al., J. Biochem. 164 (3): 223-229, 2018)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

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          RDB16707 GFP-OPTN-WT DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Kitamura, A., Determination of cytoplasmic optineurin foci sizes using image correlation spectroscopy. J. Biochem. 164 (3): 223-229 (2018). PMID 29684153.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16707_A8Ilp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: VenusC_F2
          Sequence file: RDB16707_A8Ila.seq check
          >D05635A1_A8Il__3_VenusC_F2_A01_01_ABI08.ab1
              1 AAGCAGGGGA AAGCGGCCGG GATCACTCTC GGCATGGACG AGCTGTACAA GTCCGGACTC
             61 AGATCTCGAG CTCAAGCTTC GAATTCTATG TCCCATCAAC CTCTCAGCTG CCTCACTGAA
            121 AAGGAGGACA GCCCCAGTGA AAGCACAGGA AATGGACCCC CCCACCTGGC CCACCCAAAC
            181 CTGGACACGT TTACCCCGGA GGAGCTGCTG CAGCAGATGA AAGAGCTCCT GACCGAGAAC
            241 CACCAGCTGA AAGAAGCCAT GAAGCTAAAT AATCAAGCCA TGAAAGGGAG ATTTGAGGAG
            301 CTTTCGGCCT GGACAGAGAA ACAGAAGGAA GAACGCCAGT TTTTTGAGAT ACAGAGCAAA
            361 GAAGCAAAAG AGCGTCTAAT GGCCTTGAGT CATGAGAATG AGAAATTGAA GGAAGAGCTT
            421 GGAAAACTAA AAGGGAAATC AGAAAGGTCA TCTGAGGACC CCACTGATGA CTCCAGGCTT
            481 CCCAGGGCCG AAGCGGAGCA GGAAAAGGAC CAGCTCAGGA CCCAGGTGGT GAGGCTACAA
            541 GCAGAGAAGG CAGACCTGTT GGGCATCGTG TCTGAACTGC AGCTCAAGCT GAACTCCAGC
            601 GGCTCCTCAG AAGATTCCTT TGTTGAAATT AGGATGGCTG AAGGAGAAGC AGAAGGGTCA
            661 GTAAAAGAAA TCAAGCATAG TCCTGGGCCC ACGAGAACAG TCTCCACTGG CACGGCATTG
            721 TCTAAATATA GGAGCAGATC TGCAGATGGG GCCAAGAATT ACTTCGAACA TGAGGAGTTA
            781 ACTGTGAGCC AGCTCCTGCT GTGCCTAAGG GAAGGGAATC AGAAGGTGGA GAGACTTGAA
            841 GTTGCACTCA AGGAGGCCAA AGAAAGAGTT TCAGATTTTG AAAAGAAAAC AAGTAATCGT
            901 TCTGAGATTG AAACCCAGAC AGAGGGGAGC ACAGAGAAAG AGAATGATGA AGAGAAAGGC
            961 CCGGAGACTG TTGGAAAGCG AAGTGGAAGC ACTGAACCCT CCAGGTGACA TCTCTGTTTA
           1021 AGGAGCTTCA AGGAGGCTCA ATACAAACTC AGCGAAGCTG AAGCTATGAG AGGAGACTTC
          1081 AAGAAAGTGT CAGGCTGTTG AAGAAACTTC TGCAATTTCC CAA
          //
          Primer: SV40polyA-R
          Sequence file: RDB16707_A8Ilb.seq check
          > GAAAGGGAGGTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTATGGCTGATTATGATCAGTTATCTAGATCCGGTGGAT
              1 CCCGAATGAT GCAATCCATC ACGTGAATCT GTAACGTGTC TATGTCAGGC AGAACCTCTC
             61 CACACTTGGG GCAGGAATGA ATCGGAATAT TCCGCTGTTG CCGCCAGTCC CTGTCCTCAG
            121 CTCCTCTTTG AACAAGGTAA GCCTGCTGGT CAGAGTCACT TGTTCTCGCC CCATGACGAC
            181 TCTGCATCTC CATCAAGGAC TGCCTGCCTC CGTCTTCGAA AGCATCATTC TCTTTCAGCA
            241 GAACTGCCAG CTGCAATGCC AGTTGCTCCT TTTCCTCATG AATTTTCTCT CTCGCTGCTC
            301 TTTCAGCATG AAAATCAGAA CAGTAAACTT CCATCTGAGC CCTGAGGATG GTCATGGTTT
            361 CCAGGTCCTC TTCCTGCTTG GCAATGGTTT GCTTCATTTC ATCCATTTGC AGCTGTTTGG
            421 AAGCCAGAGC CTTCTCTGCC AGTTCCAGTT TTTCACTCAG TTCCTTCAGC ACTGCCCTGT
            481 CCACTTTTTC TGACTCTTTT CTTGTTAGTT CCTCAATTGT TTTCAATGCA TTATTATGTT
            541 CTTGAAGAAG CTTGTTGTGT GTCATCTGTA GCACAGTTAA TTTGGACTTT TCATCCTCTG
            601 TTTTAGCCTG TTCCATTTTG ATTTCTGATA GCATGCTTTC CACTTGTAGC TCTAACTTTT
            661 TGTTAGTATA AACAAGCTCT TGCTTTTCAT TCAACTCTGA TGGAATTGCA GAATTTTTCC
            721 TTTCAAGGGC CTGACACTTT TCTTGAAGTC TCTTCTTCAT TAGCTCAGCT TCGCTGAGTT
            781 TTGTATGAGC CTCTTGAAGC TCCTTAAACA GAGATGTCAC CTGGAGGTTC AGTGCTTCCA
            841 CTTCGCTTCC AACAGTCTCC GGGCCTTTCT CTTCATCATT CTCTTTCTCT GTGCTCCCTC
            901 TGTCTGGGTT TCAATCTCAG AACGRATTAC TTGGTTTTCT TTTCAAAATC TGAAAACTCT
            961 TTCTTTGGCC TCCTTGARTG CAACTTCAAG TCTCTCCACC TTCTGGATCC TCCTAGCACA
           1021 GCAGGAGCTG GCTCACARGT TACTCCTCAT GTTCGAAGTA ATTCTTGGCA CAATCYGTGC
          1081 MGAAAATCTK GCC
          //
          Primer: SV40pro_F_V2
          Sequence file: RDB16707_A8Ilc.seq check
          >D05635A1-3_A8Il_SV40pro_F_V2_C01_07_ABI24.ab1
              1 ATAAGAGCTT TTTTGGAGCC TAGGCTTTTG CAAAGATCGA TCAAGAGACA GGATGAGGAT
             61 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA
            121 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC
            181 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA
            241 ATGAACTGCA AGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG
            301 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC
            361 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG
            421 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA
            481 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC
            541 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGAGCA
            601 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG
            661 TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT
            721 ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG
            781 ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC
            841 GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGTTC GAAATGACCG ACCAAGCGAC
            901 GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT
            961 CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA
           1021 GTTCTTCGCC CACCCTAGGG GAGGCTAACT GAAAACACGG AGGAGACATA CCGGGAGGAC
           1081 CCGCGCTATG ACGGCAATAA AAAAGACGGA TAAACGGCAC GGTGTGGGTC GGTTGTCATA
          1141 AACGCGGGGA TTCGGGTTC
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Kitamura, A., Determination of cytoplasmic optineurin foci sizes using image correlation spectroscopy. J. Biochem. 164 (3): 223-229 (2018). PMID 29684153.
          protocol Kitamura, A., Spatial Image Correlation Spectroscopy (ICS): A Technique for Average Size Determination of Subcellular Accumulated Structures from Fluorescence Microscopic Images. Bio-protocol 10(10): e3624 (2020). PMID 33659297.

          2022.07.13

          GNP_filter3_RDBDEP_html_220713.pl