Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCDNA5-Frt-Fucci2a

Flp/In targeting construct of Fucci2a

Catalog number RDB16056
Resource name pCDNA5-Frt-Fucci2a
Clone info. Flp/In targeting construct to make a single insertion into Flp/In compatible cell lines of Fucci2a a multicistronic version of Fucci2 composed of mVenus-hGem(1/110) and mCherry-hCdt1(30/120) fused with t2a.
Comment This clone was used in generating stable cell lines by the depositor.
Vector backbone pCDNA5/Frt (Plasmid)
Size of vector backbone 5.0 kb
Selectable markers Ampicillin (E. coli), Hygromycin (mammalian cells)
Gene/insert name Human CDT1 cDNA Human Gem cDNA Discosoma sp. RFP cDNA Aequorea victoria GFP cDNA
Depositor|Developer Jackson, Ian | Mort, Richard |
Other clones in our bank

External Database
Human CDT1 Human Gem Discosoma sp. RFP
    Aequorea victoria GFP

        Reference sequence
          

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions set forth by the DEPOSITOR 1) The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers and the BIOLOGICAL RESOURCE may not be used on humans.
        2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR (Mort, R.L. et al., Cell Cycle 13 (17): 2681-2696, (2014)) is requested.
        3) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and Dr. Richard Mort of Lancaster University is requested.
        4) The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
        5) The BIOLOGICAL RESOURCE may not be used for commercial purposes by the RECIPIENT.
        6) The RECIPIENT may not transfer the BIOLOGICAL RESOURCE to any third parties.
        Additional terms and conditions for distribution RECIPIENT is requested to obtain written consent of Dr. Miyawaki, Team of the RIKEN CBS regarding Venus.
        Ordering Please visit Information of Request for Distribution.[link] 
        Order form 
        [Credit Card Payment]  [Bank Transfer Payment]
        Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
        Approval form [Word] by Dr. Atsushi Miyawaki
        Remarks
        This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
        Please obtain written approval of Dr. Atsushi Miyawaki.
        提供案内 (日本国内) [open/close]

        提供条件 1) The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers and the BIOLOGICAL RESOURCE may not be used on humans.
        2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR (Mort, R.L. et al., Cell Cycle 13 (17): 2681-2696, (2014)) is requested.
        3) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and Dr. Richard Mort of Lancaster University is requested.
        4) The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
        5) The BIOLOGICAL RESOURCE may not be used for commercial purposes by the RECIPIENT.
        6) The RECIPIENT may not transfer the BIOLOGICAL RESOURCE to any third parties.
        付加的提供条件 利用者は、Venusについて理化学研究所CBS 宮脇敦史博士から、書面による提供承諾を得ること。
        提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
        提供依頼書 [Word]
        提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
        宮脇敦史博士による提供承諾書 [Word]
        備考 提供条件の言語は英語のみ。
        Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。
        宮脇敦史博士の書面による提供承諾が必要です。

        Catalog # Resource name Shipping form Fee (non-profit org.)
        RDB16056 pCDNA5-Frt-Fucci2a DNA solution

        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        References and tips

        Electronic file

        Original reference

        original Mort, R.L., Fucci2a: a bicistronic cell cycle reporter that allows Cre mediated tissue specific expression in mice. Cell Cycle 13 (17): 2681-2696 (2014). PMID 25486356.

        Further references such as user reports and related articles (go to bottom)

        Featured content

        Featured content Fucci-related materials (en) (English text)
        Featured content Fucci-related materials (ja) (Japanese text)

        Sequence information

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB16056_A9K8p1-1.pdf check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: CMV_Forward
        Region: CMV pro, T7 pro, insert 5' (mCherry)
        Sequence file: RDB16056_A9K8a.seq check
        >D05769A1_A9K8_1_CMV_Forward_A06_03_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNGCNAATGG GCGGTANGCG TGTACGGTGG GNGGTCTATA
           61 TAAGCAGAGC TCTCTGGCTA ACTAGAGAAC CCACTGCTTA CTGGCTTATC GAAATTAATA
          121 CGACTCACTA TAGGGAGACC CAAGCTGGCT AGAATTCCTC GACACGCGTA TGGTGAGCAA
          181 GGGCGAGGAG GATAACATGG CCATCATCAA GGAGTTCATG CGCTTCAAGG TGCACATGGA
          241 GGGCTCCGTG AACGGCCACG AGTTCGAGAT CGAGGGCGAG GGCGAGGGCC GCCCCTACGA
          301 GGGCACCCAG ACCGCCAAGC TGAAGGTGAC CAAGGGTGGC CCCCTGCCCT TCGCCTGGGA
          361 CATCCTGTCC CCTCAGTTCA TGTACGGCTC CAAGGCCTAC GTGAAGCACC CCGCCGACAT
          421 CCCCGACTAC TTGAAGCTGT CCTTCCCCGA GGGCTTCAAG TGGGAGCGCG TGATGAACTT
          481 CGAGGACGGC GGCGTGGTGA CCGTGACCCA GGACTCCTCC CTGCAGGACG GCGAGTTCAT
          541 CTACAAGGTG AAGCTGCGCG GCACCAACTT CCCCTCCGAC GGCCCCGTAA TGCAGAAGAA
          601 GACCATGGGC TGGGAGGCCT CCTCCGAGCG GATGTACCCC GAGGACGGCG CCCTGAAGGG
          661 CGAGATCAAG CAGAGGCTGA AGCTGAANGA CGGCGGCCAC TACGACGCTG ANGTCAAGAC
          721 CACCTACAAN GCCAAGAAGC CCGTGCAGCT GCCCGGCGCC TACAACGTCA ACATCAAGTT
          781 GGACATCACC TCCCACAACG AGGNNTACAC CATCNNNGAA CAGTACGAAC GCGCCGANGG
          841 CCGCNACTCC ACCGGCGGCA TGNNCGANCT GTACAAGGGN TATNCNTCAC ACNNGCGGNC
          901 GCNCNNNCCN AGCCCCCGCC NGGNCNGCNC TNCNNNNCCC GGNCNTNNGN NANNNNNNNN
        961 NN
        //
        Primer: BGH_rev2
        Region: BGH p(A), insert 3' (Gem1_1-110, mVenus)
        Sequence file: RDB16056_A9K8b.seq check
        >D05769A1_A9K8_1_BGH_rev2_B06_06_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNG GNNGNNGGGC AAACAACAGN TGGCTGGCAA CTAGAAGGCA
           61 CAGTCGAGGC TGATCAGCGG GTTTAAACGG CCCGGCGCGC CACATGTGGT ACCTTACAGC
          121 GCCTTTCTCC GTTTTTCTGC CACTTCCTTC CAATACTGAG AGGATGGATT TTCTTTAATC
          181 ATAAGATCAA ATGACTCCTG GGTGACTCCT CCAAGATTTT TATTTTCACT AGATTCTGGG
          241 ACAATAACCC CAGGGCTGGA AGTTGTAGAT GTTAAGTGGT CATTCCGATG TTTCCTTTTG
          301 GACAAGCCTG CGGACAGCTC ATTTTCTCTT CCAACAAGAG ATCCAGATGC AGAAGGCTGA
          361 ATCATCTTCA GAGTTCTTCT TGGGACAGAA CTATTCTTTA TATTCTCTTT GATTTCTTCT
          421 TGTTTCTGCT TCATACTGGG ATTCATCTCG AGCGGCCGCC AGTGTGATGG ATATCCCTTG
          481 TACAGCTCGT CCATGCCGAG AGTGATCCCG GCGGCGGTCA CGAACTCCAG CAGGACCATG
          541 TGATCGCGCT TCTCGTTGGG GTCTTTGCTC AGCTTGGACT GGTAGCTCAG GTAGTGGTTG
          601 TCGGGCAGCA GCACGGGGCC GTCGCCGATG GGGGTGTTCT GCTGGTAGTG GTCGGCGAGC
          661 TGCACGCCGC CGTCCTCGAT GTTGTGGCGG ATCTTGAAGT TGGCCTTGAT GCCGTTCTTC
          721 TGCTTGTCGG CGGTGATATA GACGTTGTGG CTGTTGTAGT TGTACTCCAG CTTGTGCCCC
          781 AGGATGTTGC CGTCCTCCTT GAAGTCGATG CCCTTCAGCT CGATGCGGTT CACCAGGGNG
          841 TCGCCCTCNA NCTTCACCTC NGCGCGGGNC TNNTANTTGC CGNCNNNCTT GAAGAANANG
          901 GNGCGNNNCN GGACGTANCC TTCGGGNNTG GNNGNNTNNN NNNNNNCNNG CNNNTTNNNN
        961 NNNNNN
        //
        Primer: HygR-M_R
        Region: HygR, FRT
        Sequence file: RDB16056_A9K8c.seq check
        >D05769A1_A9K8_1_HygR-M_R_A02_01_ABI08.ab1
            1 NNNNNNNNNN NNNNNNNNNN GNNCGANTCC TTGCGGTCCG AATGGGCCGA ACCCGCTCGT
           61 CTGGCTAAGA TCGGCCGCAG CGATCGCATC CATGGCCTCC GCGACCGGCT GCAGAACAGC
          121 GGGCAGTTCG GTTTCAGGCA GGTCTTGCAA CGTGACACCC TGTGCACGGC GGGAGATGCA
          181 ATAGGTCAGG CTCTCGCTGA ATTCCCCAAT GTCAAGCACT TCCGGAATCG GGAGCGCGGC
          241 CGATGCAAAG TGCCGATAAA CATAACGATC TTTGTAGAAA CCATCGGCGC AGCTATTTAC
          301 CCGCAGGACA TATCCACGCC CTCCTACATC GAAGCTGAAA GCACGAGATT CTTCGCCCTC
          361 CGAGAGCTGC ATCAGGTCGG AGACGCTGTC GAACTTTTCG ATCAGAAACT TCTCGACAGA
          421 CGTCGCGGTG AGTTCAGGCT TTTTGGCCAA GGAAGTTCCT ATACTTTCTA GAGAATAGGA
          481 ACTTCGGAAT AGGAACTTCT AGGTACGTGA ACCATCACCC TAATCAAGTT TTTTGGGGTC
          541 GAGGTGCCGT AAAGCACTAA ATCGGAACCC TAAAGGGAGC CCCCGATTTA GAGCTTGACG
          601 GGGAAAGCCG GCGAACGTGG CGAGAAAGGA AGGGAAGAAA GCGAAAGGAG CGGGCGCTAG
          661 GGCGCTGGCA AGTGTAGCGG TCACGCTGCG CGTAACCACC ACACCCGCCG CGCTTAATGC
          721 GCCGCTACAG GGCGCGTGGG GANACCCCCT AGAGCCCCAG CTGGTTCTTT CCGCCTCANA
          781 AGCCATAGAG CCCACCGCAT CCCCAGCATG CCTGCTATTG TCTTCCCNAT CCTCCCCCTT
          841 GCTGTCCTGC CCCACCCCNC CCCCCCAGAA TANAATGANA CCTACTCNGA NAATGCGATG
          901 CAATTTCCTC ATTTTATTAN GAAAGGANAG NGGGANTGGN ACCNNCCANG GNNNNNGNAN
          961 NNNNNNGGGG NAGGGNNAAN NNNNNNANGG NNNGNNNCTA NNANNNNCAG NCNNNNNNNN
        1021 NN
        //
        Primer: mCherry-M_F1
        Region: insert mid (mCherry, Cdt1_30-120)
        Sequence file: RDB16056_A9K8d.seq check
        >D05769A1_A9K8_1_mCherry-M_F1_A06_03_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN NGNNGAANAN NNCNTGGGCT GGGNNGCCTC CTCCGAGCGG
           61 ANGTACCCCG ANGNNGGCGC CCTGAAGGGC GAGATCAAGC AGAGGCTGAA GCTGAAGGAC
          121 GGCGGCCACT ACGACGCTGA GGTCAAGACC ACCTACAAGG CCAAGAAGCC CGTGCAGCTG
          181 CCCGGCGCCT ACAACGTCAA CATCAAGTTG GACATCACCT CCCACAACGA GGACTACACC
          241 ATCGTGGAAC AGTACGAACG CGCCGAGGGC CGCCACTCCA CCGGCGGCAT GGACGAGCTG
          301 TACAAGGGAT ATCCATCACA CTGGCGGCCG CTCGAGCCCA GCCCCGCCAG GCCCGCACTC
          361 CGCGCCCCGG CCTCCGCTAC CAGTGGCAGC CGCAAGCGCG CCCGCCCGCC CGCCGCCCCC
          421 GGACGCGACC AGGCCAGGCC ACCGGCCCGC AGGAGACTGC GGCTGTCGGT GGACGAGGTT
          481 TCCAGCCCCA GTACCCCCGA GGCCCCAGAC ATCCCAGCCT GCCCTTCTCC GGGCCAGAAG
          541 ATAAAGAAAT CCACCCCGGC AGCAGGTCAG CCGCCCCACC TGACATCCGC GCAGGACCAG
          601 GACACCATCC AATTGGAAGG CCGTGGCTCT CTTCTCACTT GTGGCGATGT CGAAGAGAAT
          661 CCTGGACCCA TGGTGAGCAA GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC
          721 GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCAGCGTGT CCGGCGAGGG CGANGGCGAN
          781 GCCACCTACN GCAAGCTGAN CCTGAANCTG ATCTGCACCA CCGGCAAGCT GCCCGNGCCC
          841 TGGCCCACCC TCNNGANCAC NCNNGGCTAC NGNCNGCAGN GNNNCNCCCG CNACCCCGAN
          901 CACNTGAAGC AGCACGANNN CTNCANGNNC GCCNTGCCCN NANNNNTANN NNCNNGNANC
        961 GNANCATCNN NTNNNNNGNA NNNNNNNNNN
        //
        Primer: Cdt1_F1
        Region: insert mid (T2A, mVenus)
        Sequence file: RDB16056_A9K8e.seq check
        >D05769A1_A9K8_1_Cdt1_F1_E06_15_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNCNN ANGNNNNGGN NNNCNTCNAA TTGGAAGGCC GTGGCTCTCT
           61 TCTCNCTTGT GGCGATGTCG AAGAGAATCC TGGACCCATG GTGAGCAAGG GCGAGGAGCT
          121 GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC GACGTAAACG GCCACAAGTT
          181 CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC AAGCTGACCC TGAAGCTGAT
          241 CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC GTGACCACCC TGGGCTACGG
          301 CCTGCAGTGC TTCGCCCGCT ACCCCGACCA CATGAAGCAG CACGACTTCT TCAAGTCCGC
          361 CATGCCCGAA GGCTACGTCC AGGAGCGCAC CATCTTCTTC AAGGACGACG GCAACTACAA
          421 GACCCGCGCC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG AACCGCATCG AGCTGAAGGG
          481 CATCGACTTC AAGGAGGACG GCAACATCCT GGGGCACAAG CTGGAGTACA ACTACAACAG
          541 CCACAACGTC TATATCACCG CCGACAAGCA GAAGAACGGC ATCAAGGCCA ACTTCAAGAT
          601 CCGCCACAAC ATCGAGGACG GCGGCGTGCA GCTCGCCGAC CACTACCAGC AGAACACCCC
          661 CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCTACC AGTCCAAGCT
          721 GANCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG TGACCGCCGC
          781 CNGGATCACT CTCGGCATGG NCGANCTGTA CAANGGATAT CCATCACACT NNCGGCCGCT
          841 CNANATGAAT CCCANTATGA AGCANAANNC AAGAAGAAAT CAAAGAGAAT ATAAAGAATA
        901 NTTCNGNCCC NAGAANAANN NNNNANNANN ANTCANCCNN NNNNNNNN
        //

        Please visit Sequencing and PCR primers for primer information.


        References

        Original, user report and related articles

        original Mort, R.L., Fucci2a: a bicistronic cell cycle reporter that allows Cre mediated tissue specific expression in mice. Cell Cycle 13 (17): 2681-2696 (2014). PMID 25486356.

        2022.05.17

        GNP_filter3_RDBDEP_html_220516.pl