icon below as well as clone information before placing your order.
Expression vector of sgRNA for targeting human ATM exon11 and human codon-optimized SpCsa9.
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External Database Human ATM Reference sequence |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Royba, E et al. Sci. Rep. 7(1): 5996, 2017) |
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| Ordering | Order form [Credit Card Payment  ] [Bank Transfer Payment]Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word ]Please visit Information of Request for Distribution.[link] |
| Remarks | The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations. |
[open/close]| 提供条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Royba, E et al. Sci Rep. 7(1) : 5996, 2017)。 |
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| 提供依頼 | 提供依頼書 [Word ]専用MTA（CRISPR/Cas9内包遺伝子材料専用 非営利学術目的）をお使いください [Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
| 備考 | 本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。 |
| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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| RDB15816 | pX330-U6-Chimeric_BB-CBh-hSpCas9 sgRNA ATM exon11 | DNA solution |
icon below as well as clone information before placing your order.Materials & Methods section:
| The pX330-U6-Chimeric_BB-CBh-hSpCas9 sgRNA ATM exon11 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15816). |
Reference section:
| Royba, E., Miyamoto, T., Akutsu, S.N., Hosoba, K., Tauchi, H., Kudo, Y., Tashiro, S., Yamamoto, T., Matsuura, S., Evaluation of ATM heterozygous mutations underlying individual differences in radiosensitivity using genome editing in human cultured cells. Sci. Rep. 7 (1): 5996 (2017). PMID 28729543. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Featured content
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
| Test sheet | RDB15816_A8Bqp1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
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Primer: SV40pA3' element Reverse Sequence file: RDB15816_A8Bqa.seq
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>D05203A1-5_A8Bq_SV40pA3'elementReverse_A01_01_ABI08.ab1 1 TCGTTCTAGG GGGGGGCGGG GGCCTATGGA AAACGCCAGC AACGCGGCCT TTTTACGGTT 61 CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTGAGGGCCT ATTTCCCATG ATTCCTTCAT 121 ATTTGCATAT ACGATACAAG GCTGTTAGAG AGATAATTGG AATTAATTTG ACTGTAAACA 181 CAAAGATATT AGTACAAAAT ACGTGACGTA GAAAGTAATA ATTTCTTGGG TAGTTTGCAG 241 TTTTAAAATT ATGTTTTAAA ATGGACTATC ATATGCTTAC CGTAACTTGA AAGTATTTCG 301 ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAAACAC CGCTGACCAC CAGTATAGTT 361 CCGTTTTAGA GCTAGAAATA GCAAGTTAAA ATAAGGCTAG TCCGTTATCA ACTTGAAAAA 421 GTGGCACCGA GTCGGTGCTT TTTTGTTTTA GAGCTAGAAA TAGCAAGTTA AAATAAGGCT 481 AGTCCGTTTT TAGCGCGTGC GCCAATTCTG CAGACAAATG GCTCTAGAGG TACCCGTTAC 541 ATAACTTACG GTAAATGGCC CGCCTGGCTG ACCGCCCAAC GACCCCCGCC CATTGACGTC 601 AATAGTAACG CCAATAGGGA CTTTCCATTG ACGTCAATGG GTGGAGTATT TACGGTAAAC 661 TGCCCACTTG GCAGTACATC AAGTGTATCA TATGCCAAGT ACGCCCCCTA TTGACGTCAA 721 TGACGGTAAA TGGCCCGCCT GGCATTGTGC CCAGTACATG ACCTTATGGG ACTTTCCTAC 781 TTGGCAGTAC ATCTACGTAT TAGTCATCGC TATTACCATG GTCGAGGTGA GCCCCACGTT 841 CTGCTTCACT CTCCCCATCT CCCCCCCCTC CCCACCCCCA ATTTTGTATT TATTTATTTT 901 TTTAATTATT TTGTGCAGCG ATGGGGGGCG GGGGGGGGGG GGGGGGGG // |
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Primer: RDB13535#1 Sequence file: RDB15816_A8Bqb.seq
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>D05203A1-5_A8Bq_RDB13535#1_B01_02_ABI08.ab1 1 TGGAAATTGG CCCTGAAATC ACTTTTTTTC AGGTTGGACC GGTGCCACCA TGGACTATAA 61 GGACCACGAC GGAGACTACA AGGATCATGA TATTGATTAC AAAGACGATG ACGATAAGAT 121 GGCCCCAAAG AAGAAGCGGA AGGTCGGTAT CCACGGAGTC CCAGCAGCCG ACAAGAAGTA 181 CAGCATCGGC CTGGACATCG GCACCAACTC TGTGGGCTGG GCCGTGATCA CCGACGAGTA 241 CAAGGTGCCC AGCAAGAAAT TCAAGGTGCT GGGCAACACC GACCGGCACA GCATCAAGAA 301 GAACCTGATC GGAGCCCTGC TGTTCGACAG CGGCGAAACA GCCGAGGCCA CCCGGCTGAA 361 GAGAACCGCC AGAAGAAGAT ACACCAGACG GAAGAACCGG ATCTGCTATC TGCAAGAGAT 421 CTTCAGCAAC GAGATGGCCA AGGTGGACGA CAGCTTCTTC CACAGACTGG AAGAGTCCTT 481 CCTGGTGGAA GAGGATAAGA AGCACGAGCG GCACCCCATC TTCGGCAACA TCGTGGACGA 541 GGTGGCCTAC CACGAGAAGT ACCCCACCAT CTACCACCTG AGAAAGAAAC TGGTGGACAG 601 CACCGACAAG GCCGACCTGC GGCTGATCTA TCTGGCCCTG GCCCACATGA TCAAGTTCCG 661 GGGCCACTTC CTGATCGAGG GCGACCTGAA CCCCGACAAC AGCGACGTGG ACAAGCTGTT 721 CATCCAGCTG GTGCAGACCT ACAACCAGCT GTTCGAGGAA AACCCCATCA ACGCCAGCGG 781 CGTGGACGCC AAGGCCATCC TGTCTGCCAG ACTGAGCAAG AGCAGACGGC TGGAAAATCT 841 GATCGCCCAG CTGCCCGGCG AGAAGAAGAA TGGCCTGTTC GGAAACCTGA TTGCCCTGAG 901 CCTGGGCCTG ACCCCCAACT TCAAGAGCAA CTTCGACCTG GCCGAGGATG CCAAACTGCA 961 GCTGAGCAAG GACACCTACG ACGACGACCC TGGACAACCT GCTGGCCCAG ATCGGCGACC 1021 AGTTACGCCG GACCTGTTTC TGGCCGCCAG GAACCTGTCC GACGCCCATT CCTGCTGAGC 1081 GACATCCTGA GAGTGACACG AGATCACAAG ACCCCCCTTG AGCGTCTCTT ATTGAATTCA 1141 GGAGAATTAC CGAACCGA // |
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Primer: BGH_rev2 Sequence file: RDB15816_A8Bqc.seq
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>D05203A1-5_A8Bq_BGH_rev2_C01_03_ABI08.ab1 1 CATCAAGGGT CAGGGAAGGG CACGGGGGAG GGGCAAACAA CAGATGGCTG GCAACTAGAA 61 GGCACAGTCG AGGCTGATCA GCGAGCTCTA GGAATTCTTA CTTTTTCTTT TTTGCCTGGC 121 CGGCCTTTTT CGTGGCCGCC GGCCTTTTGT CGCCTCCCAG CTGAGACAGG TCGATCCGTG 181 TCTCGTACAG GCCGGTGATG CTCTGGTGGA TCAGGGTGGC GTCCAGCACC TCTTTGGTGC 241 TGGTGTACCT CTTCCGGTCG ATGGTGGTGT CAAAGTACTT GAAGGCGGCA GGGGCTCCCA 301 GATTGGTCAG GGTAAACAGG TGGATGATAT TCTCGGCCTG CTCTCTGATG GGCTTATCCC 361 GGTGCTTGTT GTAGGCGGAC AGCACTTTGT CCAGATTAGC GTCGGCCAGG ATCACTCTCT 421 TGGAGAACTC GCTGATCTGC TCGATGATCT CGTCCAGGTA GTGCTTGTGC TGTTCCACAA 481 ACAGCTGTTT CTGCTCATTA TCCTCGGGGG AGCCCTTCAG CTTCTCATAG TGGCTGGCCA 541 GGTACAGGAA GTTCACATAT TTGGAGGGCA GGGCCAGTTC GTTTCCCTTC TGCAGTTCGC 601 CGGCAGAGGC CAGCATTCTC TTCCGGCCGT TTTCCAGCTC GAACAGGGAG TACTTAGGCA 661 GCTTGATGAT CAGGTCCTTT TTCACTTCTT TGTAGCCCTT GGCTTCCAGA AAGTCGATGG 721 GATTCTTCTC GAAGCTGCTT CTTTCCATGA TGGTGATCCC CAGCAGCTCT TTCACACTCT 781 TCAGTTTCTT GGACTTGCCC TTTTCCACTT TGGCCACCAC CAGCACAGAA TAGGCCACGG 841 TGGGGCTGTC GAAGCCGCCG TACTTCTTAG GGTCCCAGTC CTTCTTTCTG GCGATCAGCT 901 TATCGCTGTT CCTCTTGGGC AGGATAGACT CTTTGCTGAA GCCGCCTGTC TGCACCTCGG 961 TCTTTTTTCA CGATATTCAC TTGGGGCATG CTCAGCACTT TCCGCACGGT GGGCAAAATC 1021 CCGGCCCTTT ATCCCACACG AATCTCCCCG GGTTTCGCCG TTTTGTCTCG ATCAGAGACG 1081 CCTTTCGGAT CTTCGGCCCG GTTGGCCAAG GTATCTCGGT CTGAAAAAGT CCATGGAATG 1141 ATTGCCTGTT AGAAG // |
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Sequencing and PCR primers for primer information.
Original, user report and related articles
| original | Royba, E., Evaluation of ATM heterozygous mutations underlying individual differences in radiosensitivity using genome editing in human cultured cells. Sci. Rep. 7 (1): 5996 (2017). PMID 28729543. [link to RRC of NBRP] |
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2023.08.17
GNP_filter3_RDBDEP_html_230702.pl