Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pX330-U6-Chimeric_BB-CBh-hSpCas9 sgRNA ATM exon11 (#RDB15816)

Expression vector of sgRNA for targeting human ATM exon11 and human codon-optimized SpCsa9.

Clone info. Expression vector of sgRNA for targeting human ataxia-telangiectasia mutated (ATM) exon11 and human codon-optimized SpCsa9.
Vector backbone pX330-U6-Chimeric_BB-CBh-hSpCas9 (Plasmid)
Size of vector backbone 8.5 kb
Selectable markers Amp^r
Gene/insert name Human ATM genomic DNA
Depositor|Developer Matsuura, Shinya |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Royba, E et al. Sci. Rep. 7(1): 5996, 2017)
Remarks
The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
専用MTA（CRISPR/Cas9内包遺伝子材料専用 非営利学術目的）をお使いください [Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Royba, E et al. Sci Rep. 7(1) : 5996, 2017)。
備考
本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15816 pX330-U6-Chimeric_BB-CBh-hSpCas9 sgRNA ATM exon11 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pX330-U6-Chimeric_BB-CBh-hSpCas9 sgRNA ATM exon11 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15816).

Reference section:

Royba, E., Miyamoto, T., Akutsu, S.N., Hosoba, K., Tauchi, H., Kudo, Y., Tashiro, S., Yamamoto, T., Matsuura, S., Evaluation of ATM heterozygous mutations underlying individual differences in radiosensitivity using genome editing in human cultured cells. Sci. Rep. 7 (1): 5996 (2017). PMID 28729543. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15816_A8Bqp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: SV40pA3' element Reverse
Sequence file: RDB15816_A8Bqa.seq check
>D05203A1-5_A8Bq_SV40pA3'elementReverse_A01_01_ABI08.ab1
    1 TCGTTCTAGG GGGGGGCGGG GGCCTATGGA AAACGCCAGC AACGCGGCCT TTTTACGGTT
   61 CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTGAGGGCCT ATTTCCCATG ATTCCTTCAT
  121 ATTTGCATAT ACGATACAAG GCTGTTAGAG AGATAATTGG AATTAATTTG ACTGTAAACA
  181 CAAAGATATT AGTACAAAAT ACGTGACGTA GAAAGTAATA ATTTCTTGGG TAGTTTGCAG
  241 TTTTAAAATT ATGTTTTAAA ATGGACTATC ATATGCTTAC CGTAACTTGA AAGTATTTCG
  301 ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAAACAC CGCTGACCAC CAGTATAGTT
  361 CCGTTTTAGA GCTAGAAATA GCAAGTTAAA ATAAGGCTAG TCCGTTATCA ACTTGAAAAA
  421 GTGGCACCGA GTCGGTGCTT TTTTGTTTTA GAGCTAGAAA TAGCAAGTTA AAATAAGGCT
  481 AGTCCGTTTT TAGCGCGTGC GCCAATTCTG CAGACAAATG GCTCTAGAGG TACCCGTTAC
  541 ATAACTTACG GTAAATGGCC CGCCTGGCTG ACCGCCCAAC GACCCCCGCC CATTGACGTC
  601 AATAGTAACG CCAATAGGGA CTTTCCATTG ACGTCAATGG GTGGAGTATT TACGGTAAAC
  661 TGCCCACTTG GCAGTACATC AAGTGTATCA TATGCCAAGT ACGCCCCCTA TTGACGTCAA
  721 TGACGGTAAA TGGCCCGCCT GGCATTGTGC CCAGTACATG ACCTTATGGG ACTTTCCTAC
  781 TTGGCAGTAC ATCTACGTAT TAGTCATCGC TATTACCATG GTCGAGGTGA GCCCCACGTT
  841 CTGCTTCACT CTCCCCATCT CCCCCCCCTC CCCACCCCCA ATTTTGTATT TATTTATTTT
901 TTTAATTATT TTGTGCAGCG ATGGGGGGCG GGGGGGGGGG GGGGGGGG
//
Primer: RDB13535#1
Sequence file: RDB15816_A8Bqb.seq check
>D05203A1-5_A8Bq_RDB13535#1_B01_02_ABI08.ab1
    1 TGGAAATTGG CCCTGAAATC ACTTTTTTTC AGGTTGGACC GGTGCCACCA TGGACTATAA
   61 GGACCACGAC GGAGACTACA AGGATCATGA TATTGATTAC AAAGACGATG ACGATAAGAT
  121 GGCCCCAAAG AAGAAGCGGA AGGTCGGTAT CCACGGAGTC CCAGCAGCCG ACAAGAAGTA
  181 CAGCATCGGC CTGGACATCG GCACCAACTC TGTGGGCTGG GCCGTGATCA CCGACGAGTA
  241 CAAGGTGCCC AGCAAGAAAT TCAAGGTGCT GGGCAACACC GACCGGCACA GCATCAAGAA
  301 GAACCTGATC GGAGCCCTGC TGTTCGACAG CGGCGAAACA GCCGAGGCCA CCCGGCTGAA
  361 GAGAACCGCC AGAAGAAGAT ACACCAGACG GAAGAACCGG ATCTGCTATC TGCAAGAGAT
  421 CTTCAGCAAC GAGATGGCCA AGGTGGACGA CAGCTTCTTC CACAGACTGG AAGAGTCCTT
  481 CCTGGTGGAA GAGGATAAGA AGCACGAGCG GCACCCCATC TTCGGCAACA TCGTGGACGA
  541 GGTGGCCTAC CACGAGAAGT ACCCCACCAT CTACCACCTG AGAAAGAAAC TGGTGGACAG
  601 CACCGACAAG GCCGACCTGC GGCTGATCTA TCTGGCCCTG GCCCACATGA TCAAGTTCCG
  661 GGGCCACTTC CTGATCGAGG GCGACCTGAA CCCCGACAAC AGCGACGTGG ACAAGCTGTT
  721 CATCCAGCTG GTGCAGACCT ACAACCAGCT GTTCGAGGAA AACCCCATCA ACGCCAGCGG
  781 CGTGGACGCC AAGGCCATCC TGTCTGCCAG ACTGAGCAAG AGCAGACGGC TGGAAAATCT
  841 GATCGCCCAG CTGCCCGGCG AGAAGAAGAA TGGCCTGTTC GGAAACCTGA TTGCCCTGAG
  901 CCTGGGCCTG ACCCCCAACT TCAAGAGCAA CTTCGACCTG GCCGAGGATG CCAAACTGCA
  961 GCTGAGCAAG GACACCTACG ACGACGACCC TGGACAACCT GCTGGCCCAG ATCGGCGACC
 1021 AGTTACGCCG GACCTGTTTC TGGCCGCCAG GAACCTGTCC GACGCCCATT CCTGCTGAGC
 1081 GACATCCTGA GAGTGACACG AGATCACAAG ACCCCCCTTG AGCGTCTCTT ATTGAATTCA
1141 GGAGAATTAC CGAACCGA
//
Primer: BGH_rev2
Sequence file: RDB15816_A8Bqc.seq check
>D05203A1-5_A8Bq_BGH_rev2_C01_03_ABI08.ab1
    1 CATCAAGGGT CAGGGAAGGG CACGGGGGAG GGGCAAACAA CAGATGGCTG GCAACTAGAA
   61 GGCACAGTCG AGGCTGATCA GCGAGCTCTA GGAATTCTTA CTTTTTCTTT TTTGCCTGGC
  121 CGGCCTTTTT CGTGGCCGCC GGCCTTTTGT CGCCTCCCAG CTGAGACAGG TCGATCCGTG
  181 TCTCGTACAG GCCGGTGATG CTCTGGTGGA TCAGGGTGGC GTCCAGCACC TCTTTGGTGC
  241 TGGTGTACCT CTTCCGGTCG ATGGTGGTGT CAAAGTACTT GAAGGCGGCA GGGGCTCCCA
  301 GATTGGTCAG GGTAAACAGG TGGATGATAT TCTCGGCCTG CTCTCTGATG GGCTTATCCC
  361 GGTGCTTGTT GTAGGCGGAC AGCACTTTGT CCAGATTAGC GTCGGCCAGG ATCACTCTCT
  421 TGGAGAACTC GCTGATCTGC TCGATGATCT CGTCCAGGTA GTGCTTGTGC TGTTCCACAA
  481 ACAGCTGTTT CTGCTCATTA TCCTCGGGGG AGCCCTTCAG CTTCTCATAG TGGCTGGCCA
  541 GGTACAGGAA GTTCACATAT TTGGAGGGCA GGGCCAGTTC GTTTCCCTTC TGCAGTTCGC
  601 CGGCAGAGGC CAGCATTCTC TTCCGGCCGT TTTCCAGCTC GAACAGGGAG TACTTAGGCA
  661 GCTTGATGAT CAGGTCCTTT TTCACTTCTT TGTAGCCCTT GGCTTCCAGA AAGTCGATGG
  721 GATTCTTCTC GAAGCTGCTT CTTTCCATGA TGGTGATCCC CAGCAGCTCT TTCACACTCT
  781 TCAGTTTCTT GGACTTGCCC TTTTCCACTT TGGCCACCAC CAGCACAGAA TAGGCCACGG
  841 TGGGGCTGTC GAAGCCGCCG TACTTCTTAG GGTCCCAGTC CTTCTTTCTG GCGATCAGCT
  901 TATCGCTGTT CCTCTTGGGC AGGATAGACT CTTTGCTGAA GCCGCCTGTC TGCACCTCGG
  961 TCTTTTTTCA CGATATTCAC TTGGGGCATG CTCAGCACTT TCCGCACGGT GGGCAAAATC
 1021 CCGGCCCTTT ATCCCACACG AATCTCCCCG GGTTTCGCCG TTTTGTCTCG ATCAGAGACG
 1081 CCTTTCGGAT CTTCGGCCCG GTTGGCCAAG GTATCTCGGT CTGAAAAAGT CCATGGAATG
1141 ATTGCCTGTT AGAAG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Royba, E., Evaluation of ATM heterozygous mutations underlying individual differences in radiosensitivity using genome editing in human cultured cells. Sci. Rep. 7 (1): 5996 (2017). PMID 28729543. [link to RRC of NBRP]