Please review the QC test results indicated by icon below as well as clone information before placing your order.

pX330-U6-Chimeric_BB-CBh-hSpCas9 sgRNA ATM exon11

Expression vector of sgRNA for targeting human ATM exon11 and human codon-optimized SpCsa9.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Royba, E et al. Sci. Rep. 7(1): 5996, 2017)
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer or Check Payment] Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word]
Remarks	The BIOLOGICAL RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations. Please refer the Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose).

Distribution information (domestic users) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Royba, E et al. Sci Rep. 7(1) : 5996, 2017)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 専用MTA（CRISPR/Cas9内包遺伝子材料専用 非営利学術目的）をお使いください [Word]
備考	本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。専用MTA（CRISPR/Cas9内包遺伝子材料専用 非営利学術目的）をご参照ください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15816_A8Bqp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: SV40pA3' element Reverse Sequence file: RDB15816_A8Bqa.seq
>D05203A1-5_A8Bq_SV40pA3'elementReverse_A01_01_ABI08.ab1     1 TCGTTCTAGG GGGGGGCGGG GGCCTATGGA AAACGCCAGC AACGCGGCCT TTTTACGGTT    61 CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTGAGGGCCT ATTTCCCATG ATTCCTTCAT   121 ATTTGCATAT ACGATACAAG GCTGTTAGAG AGATAATTGG AATTAATTTG ACTGTAAACA   181 CAAAGATATT AGTACAAAAT ACGTGACGTA GAAAGTAATA ATTTCTTGGG TAGTTTGCAG   241 TTTTAAAATT ATGTTTTAAA ATGGACTATC ATATGCTTAC CGTAACTTGA AAGTATTTCG   301 ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAAACAC CGCTGACCAC CAGTATAGTT   361 CCGTTTTAGA GCTAGAAATA GCAAGTTAAA ATAAGGCTAG TCCGTTATCA ACTTGAAAAA   421 GTGGCACCGA GTCGGTGCTT TTTTGTTTTA GAGCTAGAAA TAGCAAGTTA AAATAAGGCT   481 AGTCCGTTTT TAGCGCGTGC GCCAATTCTG CAGACAAATG GCTCTAGAGG TACCCGTTAC   541 ATAACTTACG GTAAATGGCC CGCCTGGCTG ACCGCCCAAC GACCCCCGCC CATTGACGTC   601 AATAGTAACG CCAATAGGGA CTTTCCATTG ACGTCAATGG GTGGAGTATT TACGGTAAAC   661 TGCCCACTTG GCAGTACATC AAGTGTATCA TATGCCAAGT ACGCCCCCTA TTGACGTCAA   721 TGACGGTAAA TGGCCCGCCT GGCATTGTGC CCAGTACATG ACCTTATGGG ACTTTCCTAC   781 TTGGCAGTAC ATCTACGTAT TAGTCATCGC TATTACCATG GTCGAGGTGA GCCCCACGTT   841 CTGCTTCACT CTCCCCATCT CCCCCCCCTC CCCACCCCCA ATTTTGTATT TATTTATTTT 901 TTTAATTATT TTGTGCAGCG ATGGGGGGCG GGGGGGGGGG GGGGGGGG //
Primer: RDB13535#1 Sequence file: RDB15816_A8Bqb.seq
>D05203A1-5_A8Bq_RDB13535#1_B01_02_ABI08.ab1     1 TGGAAATTGG CCCTGAAATC ACTTTTTTTC AGGTTGGACC GGTGCCACCA TGGACTATAA    61 GGACCACGAC GGAGACTACA AGGATCATGA TATTGATTAC AAAGACGATG ACGATAAGAT   121 GGCCCCAAAG AAGAAGCGGA AGGTCGGTAT CCACGGAGTC CCAGCAGCCG ACAAGAAGTA   181 CAGCATCGGC CTGGACATCG GCACCAACTC TGTGGGCTGG GCCGTGATCA CCGACGAGTA   241 CAAGGTGCCC AGCAAGAAAT TCAAGGTGCT GGGCAACACC GACCGGCACA GCATCAAGAA   301 GAACCTGATC GGAGCCCTGC TGTTCGACAG CGGCGAAACA GCCGAGGCCA CCCGGCTGAA   361 GAGAACCGCC AGAAGAAGAT ACACCAGACG GAAGAACCGG ATCTGCTATC TGCAAGAGAT   421 CTTCAGCAAC GAGATGGCCA AGGTGGACGA CAGCTTCTTC CACAGACTGG AAGAGTCCTT   481 CCTGGTGGAA GAGGATAAGA AGCACGAGCG GCACCCCATC TTCGGCAACA TCGTGGACGA   541 GGTGGCCTAC CACGAGAAGT ACCCCACCAT CTACCACCTG AGAAAGAAAC TGGTGGACAG   601 CACCGACAAG GCCGACCTGC GGCTGATCTA TCTGGCCCTG GCCCACATGA TCAAGTTCCG   661 GGGCCACTTC CTGATCGAGG GCGACCTGAA CCCCGACAAC AGCGACGTGG ACAAGCTGTT   721 CATCCAGCTG GTGCAGACCT ACAACCAGCT GTTCGAGGAA AACCCCATCA ACGCCAGCGG   781 CGTGGACGCC AAGGCCATCC TGTCTGCCAG ACTGAGCAAG AGCAGACGGC TGGAAAATCT   841 GATCGCCCAG CTGCCCGGCG AGAAGAAGAA TGGCCTGTTC GGAAACCTGA TTGCCCTGAG   901 CCTGGGCCTG ACCCCCAACT TCAAGAGCAA CTTCGACCTG GCCGAGGATG CCAAACTGCA   961 GCTGAGCAAG GACACCTACG ACGACGACCC TGGACAACCT GCTGGCCCAG ATCGGCGACC  1021 AGTTACGCCG GACCTGTTTC TGGCCGCCAG GAACCTGTCC GACGCCCATT CCTGCTGAGC  1081 GACATCCTGA GAGTGACACG AGATCACAAG ACCCCCCTTG AGCGTCTCTT ATTGAATTCA 1141 GGAGAATTAC CGAACCGA //
Primer: BGH_rev2 Sequence file: RDB15816_A8Bqc.seq
>D05203A1-5_A8Bq_BGH_rev2_C01_03_ABI08.ab1     1 CATCAAGGGT CAGGGAAGGG CACGGGGGAG GGGCAAACAA CAGATGGCTG GCAACTAGAA    61 GGCACAGTCG AGGCTGATCA GCGAGCTCTA GGAATTCTTA CTTTTTCTTT TTTGCCTGGC   121 CGGCCTTTTT CGTGGCCGCC GGCCTTTTGT CGCCTCCCAG CTGAGACAGG TCGATCCGTG   181 TCTCGTACAG GCCGGTGATG CTCTGGTGGA TCAGGGTGGC GTCCAGCACC TCTTTGGTGC   241 TGGTGTACCT CTTCCGGTCG ATGGTGGTGT CAAAGTACTT GAAGGCGGCA GGGGCTCCCA   301 GATTGGTCAG GGTAAACAGG TGGATGATAT TCTCGGCCTG CTCTCTGATG GGCTTATCCC   361 GGTGCTTGTT GTAGGCGGAC AGCACTTTGT CCAGATTAGC GTCGGCCAGG ATCACTCTCT   421 TGGAGAACTC GCTGATCTGC TCGATGATCT CGTCCAGGTA GTGCTTGTGC TGTTCCACAA   481 ACAGCTGTTT CTGCTCATTA TCCTCGGGGG AGCCCTTCAG CTTCTCATAG TGGCTGGCCA   541 GGTACAGGAA GTTCACATAT TTGGAGGGCA GGGCCAGTTC GTTTCCCTTC TGCAGTTCGC   601 CGGCAGAGGC CAGCATTCTC TTCCGGCCGT TTTCCAGCTC GAACAGGGAG TACTTAGGCA   661 GCTTGATGAT CAGGTCCTTT TTCACTTCTT TGTAGCCCTT GGCTTCCAGA AAGTCGATGG   721 GATTCTTCTC GAAGCTGCTT CTTTCCATGA TGGTGATCCC CAGCAGCTCT TTCACACTCT   781 TCAGTTTCTT GGACTTGCCC TTTTCCACTT TGGCCACCAC CAGCACAGAA TAGGCCACGG   841 TGGGGCTGTC GAAGCCGCCG TACTTCTTAG GGTCCCAGTC CTTCTTTCTG GCGATCAGCT   901 TATCGCTGTT CCTCTTGGGC AGGATAGACT CTTTGCTGAA GCCGCCTGTC TGCACCTCGG   961 TCTTTTTTCA CGATATTCAC TTGGGGCATG CTCAGCACTT TCCGCACGGT GGGCAAAATC  1021 CCGGCCCTTT ATCCCACACG AATCTCCCCG GGTTTCGCCG TTTTGTCTCG ATCAGAGACG  1081 CCTTTCGGAT CTTCGGCCCG GTTGGCCAAG GTATCTCGGT CTGAAAAAGT CCATGGAATG 1141 ATTGCCTGTT AGAAG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2021.03.11

GNP_filter3_RDBDEP_html_210308.pl