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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEGFP-C1-mouse Rab32(Q83L) (#RDB15734)

Expression vector of mouse Rab32 constitutive active mutant EGFP-tag.

Clone info. Expression vector of mouse Rab32 constitutive active mutant (GTP-hydrolysis-deficient, Q83L), fused with EGFP at N-terminal. CMV promoter.
Comment Expression was confirmed by the depositor with fluorescence microscope. Please refer Itoh, T. et al., Genes. Cells, 11 (9): 1023-1037, 2006 (PMID 16923123) for gene cloning.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^R (mammalian cells).
Gene/insert name mouse Rab32 cDNA
Reference of insert sequence AB232614 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Itoh, T. et al. Genes Cells 11, 1023-1037, 2006); (Ishida, M. et al. J. Cell Sci. 125, 5177-5187, 2012)
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Itoh, T. et al. Genes Cells 11, 1023-1037, 2006); (Ishida, M. et al. J. Cell Sci. 125, 5177-5187, 2012)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15734 pEGFP-C1-mouse Rab32(Q83L) DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-mouse Rab32(Q83L) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15734).

Reference section:

Ishida, M., Ohbayashi, N., Maruta, Y., Ebata, Y., Fukuda, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci., 125 (Pt 21): 5177-5187 (2012). PMID 22854043. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15734_A8A9p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 (Pr0402)
Region: EGFP 3',insert
Sequence file: RDB15734_A8A9a.seq check
>D05154B2_A8A9_1_EGFP-C2_C08_08_ABI24.ab1
    1 NNNNNNNNNN NCNCGNNCNC NTGGNCCTGC TGGNNTTCGT GNNCGCCGCC GGGATCACTC
   61 TCGGCATGGA CGAGCTGTAC AAGTCCGGAC TCAGATCCAT GGCGGGCGAG GGACTAGGGC
  121 AACAGGGCGC GTCGGCCACC GCGGCGCCGG AGACCCGAGA GCACCTCTTT AAGGTGCTGG
  181 TCATCGGCGA GCTCGGCGTG GGTAAGACGA GCATCATCAA GCGCTACGTG CACCAGCTCT
  241 TCTCCCAGCA CTACCGCGCC ACCATTGGCG TGGACTTCGC CCTCAAAGTT CTCAACTGGG
  301 ACAGCAGGAC GCTCGTGCGC CTGCAGCTGT GGGATATCGC GGGACTGGAA CGGTTTGGCA
  361 ACATGACTCG AGTATACTAT AAGGAAGCTC TTGGAGCATT TGTAGTCTTT GATATATCGA
  421 GAAGTTCTAC ATTTGATGCA GTCCTAAAAT GGAAAAACGA TCTGGATAGT AAAGTTCATC
  481 TTCCCAACGG CAGCCCCATT CCTGCTGTCC TCCTAGCTAA CAAATGTGAC CAGAAGAAGG
  541 ACAACAGCCA GAGTCCTTCC CAGATGGACC AGTTTTGTAA AGACCATGGC TTCACTGGAT
  601 GGTTTGAAAC CTCTGCCAAG GATAATATAA ACATCGACGA GGCCACCCGC TTCCTAGTGG
  661 AAAACATGCT TGCAAACCAG CAAAGTTTTC CTAGTGAAGA AATCGACCTG GACAGAATTA
  721 AACTGGTAGA GGAGCCCCCC ACAACAAAGC CCAGGTCCCA GTGCTGCTGA TGTGTCTCTG
  781 TGAATCGAAT TCGATATCAA GCTTATCGAT ACCGTCGANN GTACCGCGGG CCCGGGNTCC
  841 ACCGGNNCTA GATAACTGAT CATAATCAGC CNTACCACAT TNGTAGAGGN TTNACTTGCT
  901 TTNAAAAAAC NTNCNNCANC NCCCCCCNGN ANNNTNAANN NTAAAANGAA TGNNNNNGNN
961 NNNGNNNNNN NNNTNNNNNN ANCNTNANNN NNNNTNNNNA NNNANNNNNN NNNN
//
Primer: SV40polyA_R2 (Pr0623)
Region: insert 3'
Sequence file: RDB15734_A8A9b.seq check
>D05154B2_A8A9_1_SV40polyA-R2_F01_06_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNGTTNNCAA CNACAATTGC ATTCNTTTTA TGTTTCNGGT
   61 TCAGGGGGAG GTGTGGGAGG TTTTTTAAAG CAAGTAAAAC CTCTACAAAT GTGGTATGGC
  121 TGATTATGAT CAGTTATCTA GATCCGGTGG ATCCCGGGCC CGCGGTACCG TCGACGGTAT
  181 CGATAAGCTT GATATCGAAT TCGATTCACA GAGACACATC AGCAGCACTG GGACCTGGGC
  241 TTTGTTGTGG GGGGCTCCTC TACCAGTTTA ATTCTGTCCA GGTCGATTTC TTCACTAGGA
  301 AAACTTTGCT GGTTTGCAAG CATGTTTTCC ACTAGGAAGC GGGTGGCCTC GTCGATGTTT
  361 ATATTATCCT TGGCAGAGGT TTCAAACCAT CCAGTGAAGC CATGGTCTTT ACAAAACTGG
  421 TCCATCTGGG AAGGACTCTG GCTGTTGTCC TTCTTCTGGT CACATTTGTT AGCTAGGAGG
  481 ACAGCAGGAA TGGGGCTGCC GTTGGGAAGA TGAACTTTAC TATCCAGATC GTTTTTCCAT
  541 TTTAGGACTG CATCAAATGT AGAACTTCTC GATATATCAA AGACTACAAA TGCTCCAAGA
  601 GCTTCCTTAT AGTATACTCG AGTCATGTTG CCAAACCGTT CCAGTCCCGC GATATCCCAC
  661 AGCTGCAGGC GCACGAGCGT CCTGCTGTCC CAGTTGAGAA CTTTGAGGGC GAAGTCCACG
  721 CCAATGGTGG CGCGGTAGTG CTGGGAGAAG AGCTGGTGCA CGTAGCGCTN GATGATGCTC
  781 GTCTTACCCA CGCCGANCTC GCCGATGANC AGCACCTTAA AGANGTGCTC TCGGGTCTCC
  841 NGCGCCGCGG NGGCCGANGC GCCCNGTTGC CCTANTCCCN CGCCCGCCAT GNATCTGANT
  901 CCGGANTNNN ACAGNNCNNN CNNGNNNANN NNGNNNCNNG NNGCNNNCAC GAACNCCNNC
961 NNNANNNTNN NNNNNNNCNT NNNNNNNGGG NNNNTNNNNN NNNNN
//
Primer: CMV_Forward (Pr0016)
Region: EGFP 5'
Sequence file: RDB15734_A8A9c.seq check
>D05154B2_1_A8A9_CMV-Forward_F05_17_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NGCGGNANGN GNNGNNNNGN GNNNNNNNNN
   61 NNNNNGNNNN NNNGGTTNNN NGNNNNGTCA GATCCGCTNG CGCTACCGGT CGCCACCATG
  121 GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC
  181 GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC
  241 AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC
  301 GTGACCACCC TGACCTACGG CGTGCAGTGC TTCAGCCGCT ACCCCGACCA CATGAAGCAG
  361 CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC CATCTTCTTC
  421 AAGGACGACG GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG
  481 AACCGCATCG AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT GGGGCACAAG
  541 CTGGAGTACA ACTACAACAG CCACAACGTC TATATCATGG CCGACAAGCA GAAGAACGGC
  601 ATCAAGGTGA ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCAGCGTGCA GCTCGCCGAN
  661 CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC
  721 CTGANCACCC AGTCCGCCCT GANCAAAGAC CCCAACGANA ANCGCGATCA CATGGNCCTG
  781 CTGGANTTCN NGANCGCCGC NNGGNNCANT CTCNGNATGG ANNANCTGTA NNNGTNCGGA
841 NTCNNATNNN NNGNNGGNNA GGNNNNAGGN NAACNNGNNN NNNNNNN
//
Primer: SV40pro_F_V2 (Pr0154)
Region: NeoR_KanR
Sequence file: RDB15734_A8A9d.seq check
>D05154B2_1_A8A9_SV40pro_F_V2_G05_20_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNGCTTTTGN AAGNNCGATC NAGAGANAGG NNGAGGNNCG
   61 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG
  121 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG
  181 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT
  241 GAACTGCAAG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA
  301 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG
  361 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT
  421 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA
  481 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG
  541 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGAGCATG
  601 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG
  661 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGANCGCTAT
  721 CANGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGNCGGCGA ATGGGCTGAN
  781 CGCTTCCTCG NGCTTTACGG TATCGCCGCT CCCGATTCGC ANCGCATCGC CTTCTATCGC
  841 CNTCTTGNNN AGTTCNTCTN ANCGGGANTN NNGGGNTCGA AATGANCGAN NNANNNANNN
901 CNNNCCNNNC NNNNNCNANA TTTCNANTNC NNNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ishida, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci., 125 (Pt 21): 5177-5187 (2012). PMID 22854043. [link to RRC of NBRP]
reference Itoh, T., Screening for target Rabs of TBC (Tre-2/Bub2/Cdc16) domain-containing proteins based on their Rab-binding activity. Genes Cells 11 (9): 1023-1037 (2006). PMID 16923123. [link to RRC of NBRP]