Expression vector of mouse Rab5C constitutive active mutant EGFP-tag.
Clone info. | Expression vector of mouse Rab5C constitutive active mutant (GTP-hydrolysis-deficient, Q80L), fused with EGFP at N-terminal. CMV promoter. |
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Comment | Expression was confirmed by the depositor with fluorescence microscope. Please refer Itoh, T. et al., Genes. Cells, 11 (9): 1023-1037, 2006 (PMID 16923123) for gene cloning. |
Vector backbone | pEGFP-C1 (plasmid) |
Size of vector backbone | 4.7 kb |
Selectable markers | Kan^r (E. coli), Neo^R (mammalian cells). |
Gene/insert name | mouse Rab5c cDNA |
Reference of insert sequence | AB232614 (DDBJ accession number) |
Depositor|Developer | Fukuda, Mitsunori | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Itoh, T. et al. Genes Cells 11, 1023-1037, 2006); (Ishida, M. et al. J. Cell Sci. 125, 5177-5187, 2012) |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Itoh, T. et al. Genes Cells 11, 1023-1037, 2006); (Ishida, M. et al. J. Cell Sci. 125, 5177-5187, 2012)。 |
Catalog # | Resource name | Availability | Shipping form | Fee (non-profit org.) |
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RDB15733 | pEGFP-C1-mouse Rab5C(Q80L) | Under QC test. Please contact us. | DNA solution |
JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge |
Materials & Methods section:
The pEGFP-C1-mouse Rab5C(Q80L) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15733). |
Reference section:
Ishida, M., Ohbayashi, N., Maruta, Y., Ebata, Y., Fukuda, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci., 125 (Pt 21): 5177-5187 (2012). PMID 22854043. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Original, user report and related articles
original | Ishida, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci., 125 (Pt 21): 5177-5187 (2012). PMID 22854043. [link to RRC of NBRP] |
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reference | Itoh, T., Screening for target Rabs of TBC (Tre-2/Bub2/Cdc16) domain-containing proteins based on their Rab-binding activity. Genes Cells 11 (9): 1023-1037 (2006). PMID 16923123. [link to RRC of NBRP] |
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