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pSilencer-neo-Rab5B/C

Expression vector of shRNA against mouse Rab5B/C.

Catalog number RDB15728
Resource name pSilencer-neo-Rab5B/C
Clone info. Expression vector of shRNA against mouse Rab5B/C (19-base target site 5'-GTTTGAGATCTGGGACACA-3'). U6 promoter.
Comment Expression was confirmed by the depositor with confocal microscopy. Please refer Mori, Y. et al., J. Biol. Chem., 288 (14): 9835-9847, 2013 (PMID 23430262) for gene cloning.
Vector backbone pSilencer 2.1-U6 neo (plasmid)
Size of vector backbone 4.5 kb
Selectable markers Amp^r (E. coli), Neo^R (mammalian cells).
Gene/insert name mouse Rab5a cDNA
Depositor|Developer Fukuda, Mitsunori |
Other clones in our bank
External Database
mouse Rab5a
          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Mori, Y. et al. J. Biol. Chem. 288, 9835-9847, 2013); (Azouz, N. P. et al. J. Immunol. 192, 4043-4053, 2014)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Mori, Y. et al. J. Biol. Chem. 288, 9835-9847, 2013); (Azouz, N. P. et al. J. Immunol. 192, 4043-4053, 2014)。
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          RDB15728 pSilencer-neo-Rab5B/C Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Electronic file

          Original reference

          original Azouz, N.P., Rab5 is a novel regulator of mast cell secretory granules: impact on size, cargo, and exocytosis. J. Immunol. 192 (9): 4043-4053 (2014). PMID 24696234.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Rab family protein (English text)

          Sequence information

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References

          Original, user report and related articles

          original Azouz, N.P., Rab5 is a novel regulator of mast cell secretory granules: impact on size, cargo, and exocytosis. J. Immunol. 192 (9): 4043-4053 (2014). PMID 24696234.
          reference Mori, Y., Rabex-5 protein regulates dendritic localization of small GTPase Rab17 and neurite morphogenesis in hippocampal neurons. J. Biol. Chem. 288 (14): 9835-9847 (2013). PMID 23430262.

          2022.05.17

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