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pFLAG-FIP1L1-PDGFRA-KD

Expression vector of human FIP1L1-PDGFRA (kinase-dead mutant), FLAG-tag.

Catalog number RDB15461
Resource name pFLAG-FIP1L1-PDGFRA-KD
Clone info. Expression vector of human FIP1L1-PDGFRA (kinase-dead mutant), tagged with FLAG at N-terminal.
Comment Expression was confirmed by the depositor with immunoprecipitation and immunoblotting.
Vector backbone pFLAG-CMV (plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name human FIP1L1 cDNA human PDGFRA cDNA
Depositor|Developer Kondo, Takeshi |
Other clones in our bank

External Database
human FIP1L1 human PDGFRA

        Reference sequence
          
         
        Sequence (full) RDB15461zzk02.seq provided by the depositor

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Ibata, M et al. Cancer Sci. 108 (2), 200-207, 2017).
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        提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ibata, M et al. Cancer Sci. 108 (2), 200-207, 2017)。
        提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
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        提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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        RDB15461 pFLAG-FIP1L1-PDGFRA-KD DNA solution

        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        How to cite this biological resource

        Materials & Methods section:

        The pFLAG-FIP1L1-PDGFRA-KD was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15461).

        Reference section:

        Ibata, M., Iwasaki, J., Fujioka, Y., Nakagawa, K., Darmanin, S., Onozawa, M., Hashimoto, D., Ohba, Y., Hatakeyama, S., Teshima, T., Kondo, T., Leukemogenic kinase FIP1L1-PDGFRA and a small ubiquitin-like modifier E3 ligase, PIAS1, form a positive cross-talk through their enzymatic activities. Cancer Sci. 108 (2): 200-207 (2017). PMID 27960034.

        Further references such as user reports and related articles (go to bottom)


        References and tips

        Featured content


        QC test results

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB15461_B2Cgp1-1.pdf check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: CMV_Forward (Pr0016)
        Region: CMV pro, FLAG, insert (KD) 5'
        Sequence file: RDB15461_B2Cga.seq check
        >D04855A8_B2Cg_1_ CMV_Forward_A05_02_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNCNT NGNNNCNNAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA
           61 TATAAGCAGA GCTCGTTTAG TGAACCGTCA GAATTAATTC AGATCGATCT ACCATGGACT
          121 ACAAAGACGA TGACGACAAG CTTGCGGCCG CGAATTCATC GATAGATCTG ATATCGGTAC
          181 CAGTCGACTC TAGAGCCATG TCGGCCGGCG AGGTCGAGCG CCTAGTGTCG GAGCTGAGCG
          241 GCGGGACCGG AGGGGATGAG GAGGAAGAGT GGCTCTATGG CGATGAAAAT GAAGTTGAAA
          301 GGCCAGAAGA AGAAAATGCC AGTGCTAATC CTCCATCTGG AATTGAAGAT GAAACTGCTG
          361 AAAATGGTGT ACCAAAACCG AAAGTGACTG AGACCGAAGA TGATAGTGAT AGTGACAGCG
          421 ATGATGATGA AGATGATGTT CATGTCACTA TAGGAGACAT TAAAACGGGA GCACCACAGT
          481 ATGGGAGTTA TGGTACAGCA CCTGTAAATC TTAACATCAA GACAGGGGGA AGAGTTTATG
          541 GAACTACAGG GACAAAAGTC AAAGGAGTAG ACCTTGATGC ACCTGGAAGC ATTAATGGAG
          601 TTCCACTCTT AGAGGTAGAT TTGGATTCTT TTGAAGATAA ACCATGGCGT AAACCTGGTG
          661 CTGATCTTTC TGANTATTTT AATTATGGGT TTAATGAAGA TACCTGGAAA GCTTACTGTG
          721 AAAAACAAAA GANGATACNA ATGGNANTTG AAGTTATACC AGTAACCTCT ACTACNAATN
          781 AAATTACGGN ACAGCNNGGA AGAACTGGAA ACTCNNANAA AGAAACTGNC CTTNCNTCTA
          841 CNAAANCTGA NTTTNNTTCN CCNCCTTCNT TGNNNNANAN NGGGNTTCCN NCGANCNNNA
          901 NANTACNNGG GGNANTNGAN NNNNNNGGNC NNANTATNNC TATCNNNNNA NNANNNNNNN
        961 NNN
        //
        Primer: hGHpolyA-R (Pr0350)
        Region: hGH pA, insert (KD) 3'
        Sequence file: RDB15461_B2Cgb.seq check
        >D04855A8_B2Cg_1_ hGHpolyA-R_B05_05_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNCC NNNAANANTA TAGAAGGANA CCTAGTCAGA CAAAATGATG
           61 CAACTTAATT TTATTAGGAC AAGGCTGGTG GGCACTGGAG TGGCAACTTC CAGGGCCAGG
          121 AGAGGCACTG GGGAGGGGTC ACAGGGATGC CACCCGGGAT CCATCAGGTC GAGTTACAGG
          181 AAGCTGTCTT CCACCAGGTC TGAAGAGTCT ATGCCGATGT CGTCCATCAT GTCGATGTCT
          241 TCAATGGTCT CGTCCTCTCT CTTGATGAAG GTGGAACTGC TGGAACCCGT CTCAATGGCA
          301 CTCTCTTCAG AGGTCTGCGA GCTGTGTCTG TTCCTCTTGC CCAGGTCCTC CTCCTCAGGG
          361 ACAGGGTCAA TGTCAGGCAG AGGAATGATG TAGCCACTGT CAGCGCTCAG TCTCTGCTCA
          421 TCCAGACCAC CCTCCCAGTC CTTCAGCTTG TCTTCCTCGT TTTTGTAGGT GACACCAATG
          481 TATGCATTGT CTGAGTCCAC ACGCATGCGT GCCACAGCAG GATGGTCACT CTTCAGGAAG
          541 TCCAGGTGAA TTTTTTCATA ACTCTTTTTA TATTGTCCAG GCAGCAGATT CTCCACAATC
          601 TCACTCAGGT GGTAAAAGGA GGGTCTCTTC TCCGGCTCAC TGTTCCAGCA TTTCACCATG
          661 ATCTCGTAGA CTTCACTGGT AGCGTGGTCA GGCTTGGCCA TCCGGTACCC ACTCTTGATC
          721 TTATTGNANA AAGTAGAATC CACCATCATG NCGGGGTAAN GGGTGNCACC NANGGAAAAN
          781 ANCTCCCAGA NCANNNNNCN TAANANCAGA NATCACTCNG NGTGGNGNAN ANGNTGNCAA
          841 AGANGNTCTC NNNANCCNTC CNCTTCNCNG NNANAANNGN ANTGNNNTNC NNNNNNTANT
          901 NCGANNCNNG NNTGANGNCT CNGGNNNNNN NNANNNCNNN NNNCTTNNNN NNTTTTNCNN
        961 NNNNNNNN
        //
        Primer: FIP1L1_F1 (Pr1075)
        Region: insert mid (KD)
        Sequence file: RDB15461_B2Cgc.seq check
        >D04855A8_B2Cg_1_ FIP1L1_F1_C05_08_ABI24.ab1
            1 NNNNNNNNNN NNNNTNNNAN GNAGNNTTGN NNTCTTTTGN AGANAAACCA TGGCGTAAAC
           61 CTGGTGCTGA TCTTTCTGAT TATTTTAATT ATGGGTTTAA TGAAGATACC TGGAAAGCTT
          121 ACTGTGAAAA ACAAAAGAGG ATACGAATGG GACTTGAAGT TATACCAGTA ACCTCTACTA
          181 CAAATAAAAT TACGGTACAG CAGGGAAGAA CTGGAAACTC AGAGAAAGAA ACTGCCCTTC
          241 CATCTACAAA AGCTGAGTTT ACTTCTCCTC CTTCTTTGTT CAAGACTGGG CTTCCACCGA
          301 GCAGGAGATT ACCTGGGGCA ATTGATGTTA TCGGTCAGAC TATAACTATC AGCCGAGTAG
          361 AAGGCAGGCG ACGGGCAAAT GAGAACAGCA ACATACAGCT GCCATATGAC TCAAGATGGG
          421 AGTTTCCAAG AGATGGACTA GTGCTTGGTC GGGTCTTGGG GTCTGGAGCG TTTGGGAAGG
          481 TGGTTGAAGG AACAGCCTAT GGATTAAGCC GGTCCCAACC TGTCATGAAA GTTGCAGTGA
          541 GGATGCTAAA ACCCACGGCC AGATCCAGTG AAAAACAAGC TCTCATGTCT GAACTGANGA
          601 TAATGACTCA CCTGGGGCCA CATTTGAACA TCGTAAACTT GCTGGGAGCC TGCACCAAGT
          661 CAGGNCCCAT TTACNTCATC ACAGANTATT GCTTCTATGG AGATTTGGTC NACTATTTGC
          721 ATNANAATAG GGATAGCTTC CTGANCCNCC ACCCAGANAA NCCNAAGAAA GANCTGGNNN
          781 TCTTTNGNNT GNANCNTGNT GANGAAANCA CACNGNNCTN NGNNATTTTA TCTTTTGAAA
          841 NNNNNNNGAN TACNTGNNNN TGNNNNNNNN TGANNCTACN NNNTANGTNC NNNNGNNNNA
        901 ANNNAANNAN GNTNNNNANN NNNNNNNN
        //
        Primer: FIP1L1v3_F2 (Pr0515)
        Region: insert mid (KD)
        Sequence file: RDB15461_B2Cgd.seq check
        >D04855A8_B2Cg_1_FIP1L1v3_F2_G05_20_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNGNA GGNGNNGGGC NNNTGAGAAC NGCAACATAC AGCTGCCATA
           61 TGACTCAAGA TGGGAGTTTC CAAGAGATGG ACTAGTGCTT GGTCGGGTCT TGGGGTCTGG
          121 AGCGTTTGGG AAGGTGGTTG AAGGAACAGC CTATGGATTA AGCCGGTCCC AACCTGTCAT
          181 GAAAGTTGCA GTGAGGATGC TAAAACCCAC GGCCAGATCC AGTGAAAAAC AAGCTCTCAT
          241 GTCTGAACTG AGGATAATGA CTCACCTGGG GCCACATTTG AACATCGTAA ACTTGCTGGG
          301 AGCCTGCACC AAGTCAGGCC CCATTTACAT CATCACAGAG TATTGCTTCT ATGGAGATTT
          361 GGTCAACTAT TTGCATAAGA ATAGGGATAG CTTCCTGAGC CACCACCCAG AGAAGCCAAA
          421 GAAAGAGCTG GATATCTTTG GACTGAACCC TGCTGATGAA AGCACACGGA GCTATGTTAT
          481 TTTATCTTTT GAAAACAATG GTGACTACAT GGACATGAAG CAGGCTGATA CTACACAGTA
          541 TGTCCCCATG CTAGAAAGGA AAGAGGTTTC TAAATATTCC GACATCCAGA GATCACTCTA
          601 TGATCGTCCA GCCTCATATA AGAAGAAATC TATGTTAGAC TCAGAAGTCA AAAACCTCCT
          661 TTCAGGTGAT AACTCAGAAN GCCTTACTTT ATTGGATTTG TTGAGCTTCA CCTATCAAGT
          721 TGCCCGANGA ATGGAGTTTT TGGCTTCAAA AAATTGTGTC CACCGTGATC TGGCTGCTCG
          781 CAACGTCCTC CTGGCACNAG GAAAAATTGT GAAGANCTGT GANTTTGGCA TGGCCNGAGA
          841 CNTCATGCAT GANNCGAACT ATGTGTCGGA ANGNAGTACC TTTCTGCCNN NNNANNNNNN
          901 GGNTCCNGAN ANCATCTTTG ANNNNCNCTA CACCACACTG ANTGANGTCN GGNCNNNTGG
        961 NNNNNNGNNN NNGNNNANNT TTTNCNNNNN NNNNN
        //
        Primer: PDGFRA_F1 (Pr1076)
        Region: insert mid (KD)
        Sequence file: RDB15461_B2Cge.seq check
        >D04855A8_B2Cg_1_ PDGFRA_F1_E05_14_ABI24.ab1
            1 NNNNNNNNNN NNNNCCCNNN CNNGAANNNN NNNGNTTCNN AAATATTCCG ACATCCNGAG
           61 ATCACTCTAT GATCGTCCAG CCTCATATAA GAAGAAATCT ATGTTAGACT CAGAAGTCAA
          121 AAACCTCCTT TCAGGTGATA ACTCAGAAGG CCTTACTTTA TTGGATTTGT TGAGCTTCAC
          181 CTATCAAGTT GCCCGAGGAA TGGAGTTTTT GGCTTCAAAA AATTGTGTCC ACCGTGATCT
          241 GGCTGCTCGC AACGTCCTCC TGGCACAAGG AAAAATTGTG AAGATCTGTG ACTTTGGCAT
          301 GGCCAGAGAC ATCATGCATG ATTCGAACTA TGTGTCGGAA GGCAGTACCT TTCTGCCCGT
          361 GAAGTGGATG GCTCCTGAGA GCATCTTTGA CAACCTCTAC ACCACACTGA GTGATGTCTG
          421 GTCTTATGGC ATTCTGCTCT GGGAGATCTT TTCCCTTGGT GGCACCCCTT ACCCCGGCAT
          481 GATGGTGGAT TCTACTTTCT ACAATAAGAT CAAGAGTGGG TACCGGATGG CCAAGCCTGA
          541 CCACGCTACC AGTGAAGTCT ACGAGATCAT GGTGAAATGC TGGAACAGTG AGCCGGAGAA
          601 GAGACCCTCC TTTTACCACC TGAGTGAGAT TGTGGAGAAT CTGCTGCCTG GACAATATAA
          661 AAAGAGTTAT GAAAAAATTC ACCTGGACTT CCTGAAGAGT GACCATCCTG CTGTGGCACG
          721 CATGCGTGTG GACTCAGACA ATGCATACAT TGGTGTCACC TACAAAAACG AGGAAGANAA
          781 GCTGAANGAN TGNNNNGGTG GTCTGGATGA GCAGANNCTG ANCGCTGANA GNGGCTACNT
          841 CATNCCTCTG CCTGANNTTG ANCCTGNCCC TGANGANGAG GANCTGGGNA AGANGNANAG
          901 ANNCAGCTCG NANANCTCTG NANANANTGN CNTTGANANG GGNTNCNGCA NTNCCNNCTT
          961 CNTCNANANA GANNNNNANN NNNTNGNNNA NNTCNANNTN ATNNNNNANN TNNNNNNNNN
        1021 N
        //
        Primer: SV40pro_ori_F (Pr0731)
        Region: SV40 pro_ori, NeoR 5'
        Sequence file: RDB15461_B2Cgf.seq check
        >D04855A8_B2Cg_1_ SV40pro_ori_F_D05_11_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNGC CGCCTCNNCC TCTGANCTAT TCCNGAAGTA GTGAGGAGGC
           61 TTTTTTGGAG GAATTGATCA GCTTGGGATC TGATCAAGAG ACAGGATGAG GATCGTTTCG
          121 CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT
          181 CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC
          241 AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT
          301 GCAGGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT
          361 GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA
          421 GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT
          481 GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG
          541 CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA
          601 AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGC GCATGCCCGA
          661 CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA
          721 TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGANC GCTATCANGA
          781 CATAGCGTTG GCTACCCGTG NTATTGCTGA AGANCTTGNC GGCGAATGGG CTGACCGCTT
          841 CCTCGNGCTT TACGGNATCG CCGCTCCCGA NNCGCAGCGC ATCGCCNTCT ATCGCCTNNT
          901 NGACNANTNC TTCNGANCGG GNNNCTGGGG NTCNAAATGA NCGACCNNNC NANNNCCNAN
        961 CNNNNNNTCN CNANANTTCN ANNCCNNNNN NNNNN
        //

        Please visit Sequencing and PCR primers for primer information.


        References

        Original, user report and related articles

        original Ibata, M., Leukemogenic kinase FIP1L1-PDGFRA and a small ubiquitin-like modifier E3 ligase, PIAS1, form a positive cross-talk through their enzymatic activities. Cancer Sci. 108 (2): 200-207 (2017). PMID 27960034.
        reference Iwasaki, J., FIP1L1 presence in FIP1L1-RARA or FIP1L1-PDGFRA differentially contributes to the pathogenesis of distinct types of leukemia. Ann. Hematol. 93 (9): 1473-1481 (2014). PMID 24763514.

        2023.05.01

        GNP_filter3_RDBDEP_html_230501.pl