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pFLAG-FIP1L1-PDGFRA-KD (#RDB15461)

Expression vector of human FIP1L1-PDGFRA (kinase-dead mutant), FLAG-tag.

Clone info. Expression vector of human FIP1L1-PDGFRA (kinase-dead mutant), tagged with FLAG at N-terminal.
Comment Expression was confirmed by the depositor with immunoprecipitation and immunoblotting.
Vector backbone pFLAG-CMV (plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name human FIP1L1 cDNA human PDGFRA cDNA
Depositor|Developer Kondo, Takeshi |
 
Sequence (full) RDB15461zzk02.seq provided by the depositor

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Ibata, M et al. Cancer Sci. 108 (2), 200-207, 2017).
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する（Ibata, M et al. Cancer Sci. 108 (2), 200-207, 2017）。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15461 pFLAG-FIP1L1-PDGFRA-KD DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pFLAG-FIP1L1-PDGFRA-KD was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15461).

Reference section:

Ibata, M., Iwasaki, J., Fujioka, Y., Nakagawa, K., Darmanin, S., Onozawa, M., Hashimoto, D., Ohba, Y., Hatakeyama, S., Teshima, T., Kondo, T., Leukemogenic kinase FIP1L1-PDGFRA and a small ubiquitin-like modifier E3 ligase, PIAS1, form a positive cross-talk through their enzymatic activities. Cancer Sci. 108 (2): 200-207 (2017). PMID 27960034. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15461_B2Cgp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro, FLAG, insert (KD) 5'
Sequence file: RDB15461_B2Cga.seq check
>D04855A8_B2Cg_1_ CMV_Forward_A05_02_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNCNT NGNNNCNNAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA
   61 TATAAGCAGA GCTCGTTTAG TGAACCGTCA GAATTAATTC AGATCGATCT ACCATGGACT
  121 ACAAAGACGA TGACGACAAG CTTGCGGCCG CGAATTCATC GATAGATCTG ATATCGGTAC
  181 CAGTCGACTC TAGAGCCATG TCGGCCGGCG AGGTCGAGCG CCTAGTGTCG GAGCTGAGCG
  241 GCGGGACCGG AGGGGATGAG GAGGAAGAGT GGCTCTATGG CGATGAAAAT GAAGTTGAAA
  301 GGCCAGAAGA AGAAAATGCC AGTGCTAATC CTCCATCTGG AATTGAAGAT GAAACTGCTG
  361 AAAATGGTGT ACCAAAACCG AAAGTGACTG AGACCGAAGA TGATAGTGAT AGTGACAGCG
  421 ATGATGATGA AGATGATGTT CATGTCACTA TAGGAGACAT TAAAACGGGA GCACCACAGT
  481 ATGGGAGTTA TGGTACAGCA CCTGTAAATC TTAACATCAA GACAGGGGGA AGAGTTTATG
  541 GAACTACAGG GACAAAAGTC AAAGGAGTAG ACCTTGATGC ACCTGGAAGC ATTAATGGAG
  601 TTCCACTCTT AGAGGTAGAT TTGGATTCTT TTGAAGATAA ACCATGGCGT AAACCTGGTG
  661 CTGATCTTTC TGANTATTTT AATTATGGGT TTAATGAAGA TACCTGGAAA GCTTACTGTG
  721 AAAAACAAAA GANGATACNA ATGGNANTTG AAGTTATACC AGTAACCTCT ACTACNAATN
  781 AAATTACGGN ACAGCNNGGA AGAACTGGAA ACTCNNANAA AGAAACTGNC CTTNCNTCTA
  841 CNAAANCTGA NTTTNNTTCN CCNCCTTCNT TGNNNNANAN NGGGNTTCCN NCGANCNNNA
  901 NANTACNNGG GGNANTNGAN NNNNNNGGNC NNANTATNNC TATCNNNNNA NNANNNNNNN
961 NNN
//
Primer: hGHpolyA-R (Pr0350)
Region: hGH pA, insert (KD) 3'
Sequence file: RDB15461_B2Cgb.seq check
>D04855A8_B2Cg_1_ hGHpolyA-R_B05_05_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNCC NNNAANANTA TAGAAGGANA CCTAGTCAGA CAAAATGATG
   61 CAACTTAATT TTATTAGGAC AAGGCTGGTG GGCACTGGAG TGGCAACTTC CAGGGCCAGG
  121 AGAGGCACTG GGGAGGGGTC ACAGGGATGC CACCCGGGAT CCATCAGGTC GAGTTACAGG
  181 AAGCTGTCTT CCACCAGGTC TGAAGAGTCT ATGCCGATGT CGTCCATCAT GTCGATGTCT
  241 TCAATGGTCT CGTCCTCTCT CTTGATGAAG GTGGAACTGC TGGAACCCGT CTCAATGGCA
  301 CTCTCTTCAG AGGTCTGCGA GCTGTGTCTG TTCCTCTTGC CCAGGTCCTC CTCCTCAGGG
  361 ACAGGGTCAA TGTCAGGCAG AGGAATGATG TAGCCACTGT CAGCGCTCAG TCTCTGCTCA
  421 TCCAGACCAC CCTCCCAGTC CTTCAGCTTG TCTTCCTCGT TTTTGTAGGT GACACCAATG
  481 TATGCATTGT CTGAGTCCAC ACGCATGCGT GCCACAGCAG GATGGTCACT CTTCAGGAAG
  541 TCCAGGTGAA TTTTTTCATA ACTCTTTTTA TATTGTCCAG GCAGCAGATT CTCCACAATC
  601 TCACTCAGGT GGTAAAAGGA GGGTCTCTTC TCCGGCTCAC TGTTCCAGCA TTTCACCATG
  661 ATCTCGTAGA CTTCACTGGT AGCGTGGTCA GGCTTGGCCA TCCGGTACCC ACTCTTGATC
  721 TTATTGNANA AAGTAGAATC CACCATCATG NCGGGGTAAN GGGTGNCACC NANGGAAAAN
  781 ANCTCCCAGA NCANNNNNCN TAANANCAGA NATCACTCNG NGTGGNGNAN ANGNTGNCAA
  841 AGANGNTCTC NNNANCCNTC CNCTTCNCNG NNANAANNGN ANTGNNNTNC NNNNNNTANT
  901 NCGANNCNNG NNTGANGNCT CNGGNNNNNN NNANNNCNNN NNNCTTNNNN NNTTTTNCNN
961 NNNNNNNN
//
Primer: FIP1L1_F1 (Pr1075)
Region: insert mid (KD)
Sequence file: RDB15461_B2Cgc.seq check
>D04855A8_B2Cg_1_ FIP1L1_F1_C05_08_ABI24.ab1
    1 NNNNNNNNNN NNNNTNNNAN GNAGNNTTGN NNTCTTTTGN AGANAAACCA TGGCGTAAAC
   61 CTGGTGCTGA TCTTTCTGAT TATTTTAATT ATGGGTTTAA TGAAGATACC TGGAAAGCTT
  121 ACTGTGAAAA ACAAAAGAGG ATACGAATGG GACTTGAAGT TATACCAGTA ACCTCTACTA
  181 CAAATAAAAT TACGGTACAG CAGGGAAGAA CTGGAAACTC AGAGAAAGAA ACTGCCCTTC
  241 CATCTACAAA AGCTGAGTTT ACTTCTCCTC CTTCTTTGTT CAAGACTGGG CTTCCACCGA
  301 GCAGGAGATT ACCTGGGGCA ATTGATGTTA TCGGTCAGAC TATAACTATC AGCCGAGTAG
  361 AAGGCAGGCG ACGGGCAAAT GAGAACAGCA ACATACAGCT GCCATATGAC TCAAGATGGG
  421 AGTTTCCAAG AGATGGACTA GTGCTTGGTC GGGTCTTGGG GTCTGGAGCG TTTGGGAAGG
  481 TGGTTGAAGG AACAGCCTAT GGATTAAGCC GGTCCCAACC TGTCATGAAA GTTGCAGTGA
  541 GGATGCTAAA ACCCACGGCC AGATCCAGTG AAAAACAAGC TCTCATGTCT GAACTGANGA
  601 TAATGACTCA CCTGGGGCCA CATTTGAACA TCGTAAACTT GCTGGGAGCC TGCACCAAGT
  661 CAGGNCCCAT TTACNTCATC ACAGANTATT GCTTCTATGG AGATTTGGTC NACTATTTGC
  721 ATNANAATAG GGATAGCTTC CTGANCCNCC ACCCAGANAA NCCNAAGAAA GANCTGGNNN
  781 TCTTTNGNNT GNANCNTGNT GANGAAANCA CACNGNNCTN NGNNATTTTA TCTTTTGAAA
  841 NNNNNNNGAN TACNTGNNNN TGNNNNNNNN TGANNCTACN NNNTANGTNC NNNNGNNNNA
901 ANNNAANNAN GNTNNNNANN NNNNNNNN
//
Primer: FIP1L1v3_F2 (Pr0515)
Region: insert mid (KD)
Sequence file: RDB15461_B2Cgd.seq check
>D04855A8_B2Cg_1_FIP1L1v3_F2_G05_20_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNGNA GGNGNNGGGC NNNTGAGAAC NGCAACATAC AGCTGCCATA
   61 TGACTCAAGA TGGGAGTTTC CAAGAGATGG ACTAGTGCTT GGTCGGGTCT TGGGGTCTGG
  121 AGCGTTTGGG AAGGTGGTTG AAGGAACAGC CTATGGATTA AGCCGGTCCC AACCTGTCAT
  181 GAAAGTTGCA GTGAGGATGC TAAAACCCAC GGCCAGATCC AGTGAAAAAC AAGCTCTCAT
  241 GTCTGAACTG AGGATAATGA CTCACCTGGG GCCACATTTG AACATCGTAA ACTTGCTGGG
  301 AGCCTGCACC AAGTCAGGCC CCATTTACAT CATCACAGAG TATTGCTTCT ATGGAGATTT
  361 GGTCAACTAT TTGCATAAGA ATAGGGATAG CTTCCTGAGC CACCACCCAG AGAAGCCAAA
  421 GAAAGAGCTG GATATCTTTG GACTGAACCC TGCTGATGAA AGCACACGGA GCTATGTTAT
  481 TTTATCTTTT GAAAACAATG GTGACTACAT GGACATGAAG CAGGCTGATA CTACACAGTA
  541 TGTCCCCATG CTAGAAAGGA AAGAGGTTTC TAAATATTCC GACATCCAGA GATCACTCTA
  601 TGATCGTCCA GCCTCATATA AGAAGAAATC TATGTTAGAC TCAGAAGTCA AAAACCTCCT
  661 TTCAGGTGAT AACTCAGAAN GCCTTACTTT ATTGGATTTG TTGAGCTTCA CCTATCAAGT
  721 TGCCCGANGA ATGGAGTTTT TGGCTTCAAA AAATTGTGTC CACCGTGATC TGGCTGCTCG
  781 CAACGTCCTC CTGGCACNAG GAAAAATTGT GAAGANCTGT GANTTTGGCA TGGCCNGAGA
  841 CNTCATGCAT GANNCGAACT ATGTGTCGGA ANGNAGTACC TTTCTGCCNN NNNANNNNNN
  901 GGNTCCNGAN ANCATCTTTG ANNNNCNCTA CACCACACTG ANTGANGTCN GGNCNNNTGG
961 NNNNNNGNNN NNGNNNANNT TTTNCNNNNN NNNNN
//
Primer: PDGFRA_F1 (Pr1076)
Region: insert mid (KD)
Sequence file: RDB15461_B2Cge.seq check
>D04855A8_B2Cg_1_ PDGFRA_F1_E05_14_ABI24.ab1
    1 NNNNNNNNNN NNNNCCCNNN CNNGAANNNN NNNGNTTCNN AAATATTCCG ACATCCNGAG
   61 ATCACTCTAT GATCGTCCAG CCTCATATAA GAAGAAATCT ATGTTAGACT CAGAAGTCAA
  121 AAACCTCCTT TCAGGTGATA ACTCAGAAGG CCTTACTTTA TTGGATTTGT TGAGCTTCAC
  181 CTATCAAGTT GCCCGAGGAA TGGAGTTTTT GGCTTCAAAA AATTGTGTCC ACCGTGATCT
  241 GGCTGCTCGC AACGTCCTCC TGGCACAAGG AAAAATTGTG AAGATCTGTG ACTTTGGCAT
  301 GGCCAGAGAC ATCATGCATG ATTCGAACTA TGTGTCGGAA GGCAGTACCT TTCTGCCCGT
  361 GAAGTGGATG GCTCCTGAGA GCATCTTTGA CAACCTCTAC ACCACACTGA GTGATGTCTG
  421 GTCTTATGGC ATTCTGCTCT GGGAGATCTT TTCCCTTGGT GGCACCCCTT ACCCCGGCAT
  481 GATGGTGGAT TCTACTTTCT ACAATAAGAT CAAGAGTGGG TACCGGATGG CCAAGCCTGA
  541 CCACGCTACC AGTGAAGTCT ACGAGATCAT GGTGAAATGC TGGAACAGTG AGCCGGAGAA
  601 GAGACCCTCC TTTTACCACC TGAGTGAGAT TGTGGAGAAT CTGCTGCCTG GACAATATAA
  661 AAAGAGTTAT GAAAAAATTC ACCTGGACTT CCTGAAGAGT GACCATCCTG CTGTGGCACG
  721 CATGCGTGTG GACTCAGACA ATGCATACAT TGGTGTCACC TACAAAAACG AGGAAGANAA
  781 GCTGAANGAN TGNNNNGGTG GTCTGGATGA GCAGANNCTG ANCGCTGANA GNGGCTACNT
  841 CATNCCTCTG CCTGANNTTG ANCCTGNCCC TGANGANGAG GANCTGGGNA AGANGNANAG
  901 ANNCAGCTCG NANANCTCTG NANANANTGN CNTTGANANG GGNTNCNGCA NTNCCNNCTT
  961 CNTCNANANA GANNNNNANN NNNTNGNNNA NNTCNANNTN ATNNNNNANN TNNNNNNNNN
1021 N
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori, NeoR 5'
Sequence file: RDB15461_B2Cgf.seq check
>D04855A8_B2Cg_1_ SV40pro_ori_F_D05_11_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNGC CGCCTCNNCC TCTGANCTAT TCCNGAAGTA GTGAGGAGGC
   61 TTTTTTGGAG GAATTGATCA GCTTGGGATC TGATCAAGAG ACAGGATGAG GATCGTTTCG
  121 CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT
  181 CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC
  241 AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT
  301 GCAGGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT
  361 GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA
  421 GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT
  481 GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG
  541 CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA
  601 AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGC GCATGCCCGA
  661 CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA
  721 TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGANC GCTATCANGA
  781 CATAGCGTTG GCTACCCGTG NTATTGCTGA AGANCTTGNC GGCGAATGGG CTGACCGCTT
  841 CCTCGNGCTT TACGGNATCG CCGCTCCCGA NNCGCAGCGC ATCGCCNTCT ATCGCCTNNT
  901 NGACNANTNC TTCNGANCGG GNNNCTGGGG NTCNAAATGA NCGACCNNNC NANNNCCNAN
961 CNNNNNNTCN CNANANTTCN ANNCCNNNNN NNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ibata, M., Leukemogenic kinase FIP1L1-PDGFRA and a small ubiquitin-like modifier E3 ligase, PIAS1, form a positive cross-talk through their enzymatic activities. Cancer Sci. 108 (2): 200-207 (2017). PMID 27960034. [link to RRC of NBRP]
reference Iwasaki, J., FIP1L1 presence in FIP1L1-RARA or FIP1L1-PDGFRA differentially contributes to the pathogenesis of distinct types of leukemia. Ann. Hematol. 93 (9): 1473-1481 (2014). PMID 24763514.