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pEGFP-C1-mouse Rab33B-T47N (#RDB15381)

Expression vector of mouse RAB33B constitutive negative mutant T47N, N-terminal EGFP-tag.

Clone info. Expression vector of mouse RAB33B constitutive negative mutant (T47N), fused with N-terminal EGFP, CMV promoter.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name mouse Rab33b cDNA
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Itoh, T. et al., Mol. Biol. Cell, 19 (7): 2916-2925, 2008)
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Itoh, T. et al., Mol. Biol. Cell, 19 (7): 2916-2925, 2008)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15381 pEGFP-C1-mouse Rab33B-T47N DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-mouse Rab33B-T47N was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15381).

Reference section:

Itoh, T., Fujita, N., Kanno, E., Yamamoto, A., Yoshimori, T., Fukuda, M., Golgi-resident small GTPase Rab33B interacts with Atg16L and modulates autophagosome formation. Mol. Biol. Cell 19 (7): 2916-2925 (2008). PMID 18448665. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15381_A7Ibp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C
Sequence file: RDB15381_A7Iba.seq check
>D04916C6_A7Ib_1_EGFP-C_A03_03_ABI24.ab1
    1 CCGTAATCTC GGCATGGACG AGCTGTACAA GTCCGGACTC AGATCCATGA CTTCGGAGAT
   61 GGAGTCGTCG CTGGAGGTCA GCTTCTCGTC CAGCTGTGCG GTGTCAGGGG CGTCTGGGTG
  121 TTTGCCTCCC GCCCGCTCCC GCATCTTCAA GATCATAGTG ATCGGCGACT CGAACGTGGG
  181 CAAGAACTGC CTGACTTATC GATTCTGCGC CGGCCGCTTC CCCGACCGCA CCGAGGCCAC
  241 GATCGGGGTG GACTTCCGAG AGCGAGCCGT GGATATTGAT GGAGAGCGCA TCAAGATCCA
  301 GTTGTGGGAC ACGGCAGGGC AGGAGCGGTT CAGGAAGAGC ATGGTCCAGC ACTACTACAG
  361 GAATGTGCAT GCTGTCGTCT TTGTGTATGA CATGACCAAC ATGGCCAGCT TCCACAGCCT
  421 GCCGGCTTGG ATAGAGGAAT GCAAACAGCA CTTGCTGGCG AACGACATAC CTCGAATTCT
  481 GGTGGGAAAT AAATGTGACT TGAGAAGCGC CATTCAGGTG CCCACAGACT TGGCACAGAA
  541 GTTTGCTGAC ACACACAGTA TGCCTTTGTT TGAGACCTCT GCTAAAAACC CCAATGATAA
  601 TGACCACGTA GAAGCTATAT TCATGACCTT AGCTCATAAG CTAAAGAGCC ACAAGCCATT
  661 GATGCTTAGC CAGCTGCCTG ATAACAGAAT TAGCCTGAAA CCTGAGACCA AGCCTGCGGT
  721 GACTTGCTGG TGCTGAAATC ACTAGTGAAT TCGCGGCCGC CTGCAGGTCG ACGGTACCGC
  781 GGGCCCGGGA TCCACCGGAT CTAGATAACT GATCATAATC AGCCATACCA CATTTGTAGA
  841 GGTTTTACTT GCTTTAAAAA AACCTCCCAC ACCTCCCCCC TGAACCTGAA ACATAAAATG
  901 AATGCAATTG TTGTTGTTAA CTTGTTTATT GCAGCTTAAT AATGGGTTAC AAATAAAGCA
  961 ATAGCATCAC AAATTTCACA AATAAAGGCA TTTTTTTTTC ACTGCATTCT AGCTTGTCTT
1021 GTCCGAAACT CATCCATGGT ATCCTTACGC GTAAATTGTA AGCCGT
//
Primer: ME-1290RV
Sequence file: RDB15381_A7Ibb.seq check
>D04916C6_A7Ib_1_ME-1290RV_A04_01_ABI24.ab1
    1 AGGCAATTCA ATTTTATGTT TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG
   61 TAAAACCTCT ACAAATGTGG TATGGCTGAT TATGATCAGT TATCTAGATC CGGTGGATCC
  121 CGGGCCCGCG GTACCGTCGA CCTGCAGGCG GCCGCGAATT CACTAGTGAT TTCAGCACCA
  181 GCAAGTCACC GCAGGCTTGG TCTCAGGTTT CAGGCTAATT CTGTTATCAG GCAGCTGGCT
  241 AAGCATCAAT GGCTTGTGGC TCTTTAGCTT ATGAGCTAAG GTCATGAATA TAGCTTCTAC
  301 GTGGTCATTA TCATTGGGGT TTTTAGCAGA GGTCTCAAAC AAAGGCATAC TGTGTGTGTC
  361 AGCAAACTTC TGTGCCAAGT CTGTGGGCAC CTGAATGGCG CTTCTCAAGT CACATTTATT
  421 TCCCACCAGA ATTCGAGGTA TGTCGTTCGC CAGCAAGTGC TGTTTGCATT CCTCTATCCA
  481 AGCCGGCAGG CTGTGGAAGC TGGCCATGTT GGTCATGTCA TACACAAAGA CGACAGCATG
  541 CACATTCCTG TAGTAGTGCT GGACCATGCT CTTCCTGAAC CGCTCCTGCC CTGCCGTGTC
  601 CCACAACTGG ATCTTGATGC GCTCTCCATC AATATCCACG GCTCGCTCTC GGAAGTCCAC
  661 CCCGATCGTG GCCTCGGTGC GGTCGGGGAA GCGGCCGGCG CAGAATCGAT AAGTCAGGCA
  721 GTTCTTGCCC ACGTTCGAGT CGCCGATCAC TATGATCTTG AAGATGCGGG AGCGGGGCGG
  781 GGAGGCAAAC ACCCAGACGC CCCTGACACC GCACAGCTGG ACGAGAAGCT GACCTCCAGC
  841 GACGACTCCA TCTCCGAAGT CATGGGATCT GAGTCCGGAC TTGTACAGCT CGTCCATGCC
  901 GGAGAGTGAT CCCGGCGGCG GTCACGAACT CCAGCAGGAC CATGGTGATC GCGCTTCTCG
  961 TTGGGGTCTT TGCTCCAGGG CGGACTGGTG CTCAGGTAGT TGGTGTCGGG CAGCAGCACG
1021 GGGCCGGTTC GCCGATTGAG AGATGAATCT GCTTGTAAGT GTCCGGCGAA GCCTG
//
Primer: CMV-Forward
Sequence file: RDB15381_A7Ibc.seq check
>D04916C6_A7Ib_1_CMV-Forward_B04_04_ABI24.ab1
    1 CGTTCAACTC CGCCCATTGA CGCAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
   61 AGCAGAGCTG GTTTAGTGAA CCGTCAGATC CGCTAGCGCT ACCGGTCGCC ACCATGGTGA
  121 GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG TGCCCATCCT GGTCGAGCTG GACGGCGACG
  181 TAAACGGCCA CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG CGATGCCACC TACGGCAAGC
  241 TGACCCTGAA GTTCATCTGC ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA
  301 CCACCCTGAC CTACGGCGTG CAGTGCTTCA GCCGCTACCC CGACCACATG AAGCAGCACG
  361 ACTTCTTCAA GTCCGCCATG CCCGAAGGCT ACGTCCAGGA GCGCACCATC TTCTTCAAGG
  421 ACGACGGCAA CTACAAGACC CGCGCCGAGG TGAAGTTCGA GGGCGACACC CTGGTGAACC
  481 GCATCGAGCT GAAGGGCATC GACTTCAAGG AGGACGGCAA CATCCTGGGG CACAAGCTGG
  541 AGTACAACTA CAACAGCCAC AACGTCTATA TCATGGCCGA CAAGCAGAAG AACGGCATCA
  601 AGGTGAACTT CAAGATCCGC CACAACATCG AGGACGGCAG CGTGCAGCTC GCCGACCACT
  661 ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT GCCCGACAAC CACTACCTGA
  721 GCACCCAGTC CGCCCTGAGC AAAGACCCCA ACGAGAAGCG CGATCACATG GTCCTGCTGG
  781 AGTTCGTGAC CGCCGCCGGG ATCACTCTCG GCATGGACGA GCTGTACAAG TCCGGACTCA
  841 GATCCATGAC TTCGGAGATG GAGTCGTCGC TGGAGGTCAG CTTCTCGTCC AGCCTGTGCG
  901 GTGTCAGGGG CGTCTGGGTG TTTGCCTCCC GCCCGCTCCC GCATCTTTCA GATCATAGTG
  961 AATCGGCGAC TCGAAACGTG GCAGAACTGC CTGACTTATC GAATTCTGCG CCCGGCCGGC
1021 TTTCCCCGAA CCGCACCGAG GCCCACGATC GGGGGGATGG AACCT
//
Primer: SV40pro_F_V2
Sequence file: RDB15381_A7Ibd.seq check
>D04916C6_A7Ib_1_SV40pro_F_V2_C04_07_ABI24.ab1
    1 GAGGGGTTTT TTGGGAGGCT AGGCTTTTGC AAGATCGATC AAGAGACAGG ATGAGGATCG
   61 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG
  121 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG
  181 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT
  241 GAACTGCAAG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA
  301 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG
  361 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT
  421 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA
  481 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG
  541 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGAGCATG
  601 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG
  661 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT
  721 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC
  781 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC
  841 CTTCTTGACG AGTTCTTCTG AGCGGGGACT CTGGGGGTTC GAAATGACCG ACCCAAGCGA
  901 CGCCCAAACC TGCCATCACG AGATTTCGAT TCCACCCGCC GCCCTTCTAT GAAAGGTTTG
  961 GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGGATGAT CCCTCCAGCG GCGGGGGATC
 1021 TCAATGCTGG AAGTTCCTTC CGCCACCCTT AGGGGAGGCT ACCTTGAAAC ACGGAAGGGA
1081 GACC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Itoh, T., Golgi-resident small GTPase Rab33B interacts with Atg16L and modulates autophagosome formation. Mol. Biol. Cell 19 (7): 2916-2925 (2008). PMID 18448665. [link to RRC of NBRP]