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pEGFP-C1-mouse Rab33B-T47N

Expression vector of mouse RAB33B constitutive negative mutant T47N, N-terminal EGFP-tag.

Catalog number RDB15381
Resource name pEGFP-C1-mouse Rab33B-T47N
Clone info. Expression vector of mouse RAB33B constitutive negative mutant (T47N), fused with N-terminal EGFP, CMV promoter.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name mouse Rab33b cDNA
Depositor|Developer Fukuda, Mitsunori |
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External Database
mouse Rab33b

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Itoh, T. et al., Mol. Biol. Cell, 19 (7): 2916-2925, 2008)
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Itoh, T. et al., Mol. Biol. Cell, 19 (7): 2916-2925, 2008)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

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          RDB15381 pEGFP-C1-mouse Rab33B-T47N DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Itoh, T., Golgi-resident small GTPase Rab33B interacts with Atg16L and modulates autophagosome formation. Mol. Biol. Cell 19 (7): 2916-2925 (2008). PMID 18448665.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Rab family protein (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15381_A7Ibp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EGFP-C
          Sequence file: RDB15381_A7Iba.seq check
          >D04916C6_A7Ib_1_EGFP-C_A03_03_ABI24.ab1
              1 CCGTAATCTC GGCATGGACG AGCTGTACAA GTCCGGACTC AGATCCATGA CTTCGGAGAT
             61 GGAGTCGTCG CTGGAGGTCA GCTTCTCGTC CAGCTGTGCG GTGTCAGGGG CGTCTGGGTG
            121 TTTGCCTCCC GCCCGCTCCC GCATCTTCAA GATCATAGTG ATCGGCGACT CGAACGTGGG
            181 CAAGAACTGC CTGACTTATC GATTCTGCGC CGGCCGCTTC CCCGACCGCA CCGAGGCCAC
            241 GATCGGGGTG GACTTCCGAG AGCGAGCCGT GGATATTGAT GGAGAGCGCA TCAAGATCCA
            301 GTTGTGGGAC ACGGCAGGGC AGGAGCGGTT CAGGAAGAGC ATGGTCCAGC ACTACTACAG
            361 GAATGTGCAT GCTGTCGTCT TTGTGTATGA CATGACCAAC ATGGCCAGCT TCCACAGCCT
            421 GCCGGCTTGG ATAGAGGAAT GCAAACAGCA CTTGCTGGCG AACGACATAC CTCGAATTCT
            481 GGTGGGAAAT AAATGTGACT TGAGAAGCGC CATTCAGGTG CCCACAGACT TGGCACAGAA
            541 GTTTGCTGAC ACACACAGTA TGCCTTTGTT TGAGACCTCT GCTAAAAACC CCAATGATAA
            601 TGACCACGTA GAAGCTATAT TCATGACCTT AGCTCATAAG CTAAAGAGCC ACAAGCCATT
            661 GATGCTTAGC CAGCTGCCTG ATAACAGAAT TAGCCTGAAA CCTGAGACCA AGCCTGCGGT
            721 GACTTGCTGG TGCTGAAATC ACTAGTGAAT TCGCGGCCGC CTGCAGGTCG ACGGTACCGC
            781 GGGCCCGGGA TCCACCGGAT CTAGATAACT GATCATAATC AGCCATACCA CATTTGTAGA
            841 GGTTTTACTT GCTTTAAAAA AACCTCCCAC ACCTCCCCCC TGAACCTGAA ACATAAAATG
            901 AATGCAATTG TTGTTGTTAA CTTGTTTATT GCAGCTTAAT AATGGGTTAC AAATAAAGCA
            961 ATAGCATCAC AAATTTCACA AATAAAGGCA TTTTTTTTTC ACTGCATTCT AGCTTGTCTT
          1021 GTCCGAAACT CATCCATGGT ATCCTTACGC GTAAATTGTA AGCCGT
          //
          Primer: ME-1290RV
          Sequence file: RDB15381_A7Ibb.seq check
          >D04916C6_A7Ib_1_ME-1290RV_A04_01_ABI24.ab1
              1 AGGCAATTCA ATTTTATGTT TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG
             61 TAAAACCTCT ACAAATGTGG TATGGCTGAT TATGATCAGT TATCTAGATC CGGTGGATCC
            121 CGGGCCCGCG GTACCGTCGA CCTGCAGGCG GCCGCGAATT CACTAGTGAT TTCAGCACCA
            181 GCAAGTCACC GCAGGCTTGG TCTCAGGTTT CAGGCTAATT CTGTTATCAG GCAGCTGGCT
            241 AAGCATCAAT GGCTTGTGGC TCTTTAGCTT ATGAGCTAAG GTCATGAATA TAGCTTCTAC
            301 GTGGTCATTA TCATTGGGGT TTTTAGCAGA GGTCTCAAAC AAAGGCATAC TGTGTGTGTC
            361 AGCAAACTTC TGTGCCAAGT CTGTGGGCAC CTGAATGGCG CTTCTCAAGT CACATTTATT
            421 TCCCACCAGA ATTCGAGGTA TGTCGTTCGC CAGCAAGTGC TGTTTGCATT CCTCTATCCA
            481 AGCCGGCAGG CTGTGGAAGC TGGCCATGTT GGTCATGTCA TACACAAAGA CGACAGCATG
            541 CACATTCCTG TAGTAGTGCT GGACCATGCT CTTCCTGAAC CGCTCCTGCC CTGCCGTGTC
            601 CCACAACTGG ATCTTGATGC GCTCTCCATC AATATCCACG GCTCGCTCTC GGAAGTCCAC
            661 CCCGATCGTG GCCTCGGTGC GGTCGGGGAA GCGGCCGGCG CAGAATCGAT AAGTCAGGCA
            721 GTTCTTGCCC ACGTTCGAGT CGCCGATCAC TATGATCTTG AAGATGCGGG AGCGGGGCGG
            781 GGAGGCAAAC ACCCAGACGC CCCTGACACC GCACAGCTGG ACGAGAAGCT GACCTCCAGC
            841 GACGACTCCA TCTCCGAAGT CATGGGATCT GAGTCCGGAC TTGTACAGCT CGTCCATGCC
            901 GGAGAGTGAT CCCGGCGGCG GTCACGAACT CCAGCAGGAC CATGGTGATC GCGCTTCTCG
            961 TTGGGGTCTT TGCTCCAGGG CGGACTGGTG CTCAGGTAGT TGGTGTCGGG CAGCAGCACG
          1021 GGGCCGGTTC GCCGATTGAG AGATGAATCT GCTTGTAAGT GTCCGGCGAA GCCTG
          //
          Primer: CMV-Forward
          Sequence file: RDB15381_A7Ibc.seq check
          >D04916C6_A7Ib_1_CMV-Forward_B04_04_ABI24.ab1
              1 CGTTCAACTC CGCCCATTGA CGCAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
             61 AGCAGAGCTG GTTTAGTGAA CCGTCAGATC CGCTAGCGCT ACCGGTCGCC ACCATGGTGA
            121 GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG TGCCCATCCT GGTCGAGCTG GACGGCGACG
            181 TAAACGGCCA CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG CGATGCCACC TACGGCAAGC
            241 TGACCCTGAA GTTCATCTGC ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA
            301 CCACCCTGAC CTACGGCGTG CAGTGCTTCA GCCGCTACCC CGACCACATG AAGCAGCACG
            361 ACTTCTTCAA GTCCGCCATG CCCGAAGGCT ACGTCCAGGA GCGCACCATC TTCTTCAAGG
            421 ACGACGGCAA CTACAAGACC CGCGCCGAGG TGAAGTTCGA GGGCGACACC CTGGTGAACC
            481 GCATCGAGCT GAAGGGCATC GACTTCAAGG AGGACGGCAA CATCCTGGGG CACAAGCTGG
            541 AGTACAACTA CAACAGCCAC AACGTCTATA TCATGGCCGA CAAGCAGAAG AACGGCATCA
            601 AGGTGAACTT CAAGATCCGC CACAACATCG AGGACGGCAG CGTGCAGCTC GCCGACCACT
            661 ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT GCCCGACAAC CACTACCTGA
            721 GCACCCAGTC CGCCCTGAGC AAAGACCCCA ACGAGAAGCG CGATCACATG GTCCTGCTGG
            781 AGTTCGTGAC CGCCGCCGGG ATCACTCTCG GCATGGACGA GCTGTACAAG TCCGGACTCA
            841 GATCCATGAC TTCGGAGATG GAGTCGTCGC TGGAGGTCAG CTTCTCGTCC AGCCTGTGCG
            901 GTGTCAGGGG CGTCTGGGTG TTTGCCTCCC GCCCGCTCCC GCATCTTTCA GATCATAGTG
            961 AATCGGCGAC TCGAAACGTG GCAGAACTGC CTGACTTATC GAATTCTGCG CCCGGCCGGC
          1021 TTTCCCCGAA CCGCACCGAG GCCCACGATC GGGGGGATGG AACCT
          //
          Primer: SV40pro_F_V2
          Sequence file: RDB15381_A7Ibd.seq check
          >D04916C6_A7Ib_1_SV40pro_F_V2_C04_07_ABI24.ab1
              1 GAGGGGTTTT TTGGGAGGCT AGGCTTTTGC AAGATCGATC AAGAGACAGG ATGAGGATCG
             61 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG
            121 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG
            181 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT
            241 GAACTGCAAG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA
            301 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG
            361 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT
            421 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA
            481 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG
            541 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGAGCATG
            601 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG
            661 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT
            721 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC
            781 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC
            841 CTTCTTGACG AGTTCTTCTG AGCGGGGACT CTGGGGGTTC GAAATGACCG ACCCAAGCGA
            901 CGCCCAAACC TGCCATCACG AGATTTCGAT TCCACCCGCC GCCCTTCTAT GAAAGGTTTG
            961 GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGGATGAT CCCTCCAGCG GCGGGGGATC
           1021 TCAATGCTGG AAGTTCCTTC CGCCACCCTT AGGGGAGGCT ACCTTGAAAC ACGGAAGGGA
          1081 GACC
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Itoh, T., Golgi-resident small GTPase Rab33B interacts with Atg16L and modulates autophagosome formation. Mol. Biol. Cell 19 (7): 2916-2925 (2008). PMID 18448665.

          2022.07.13

          GNP_filter3_RDBDEP_html_220713.pl