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pEGFP-C1-mouse Rab33B-Q92L

Expression vector of mouse RAB33B constitutive active mutant Q92L, N-terminal EGFP-tag.

Catalog number RDB15380
Resource name pEGFP-C1-mouse Rab33B-Q92L
Clone info. Expression vector of mouse RAB33B constitutive active mutant (GTP-hydrolysis -deficient, Q92L), fused with N-terminal EGFP, CMV promoter.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name mouse Rab33b cDNA
Depositor|Developer Fukuda, Mitsunori |
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External Database
mouse Rab33b

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Itoh, T. et al., Mol. Biol. Cell, 19 (7): 2916-2925, 2008)
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Itoh, T. et al., Mol. Biol. Cell, 19 (7): 2916-2925, 2008)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

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          RDB15380 pEGFP-C1-mouse Rab33B-Q92L DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Itoh, T., Golgi-resident small GTPase Rab33B interacts with Atg16L and modulates autophagosome formation. Mol. Biol. Cell 19 (7): 2916-2925 (2008). PMID 18448665.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Rab family protein (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15380_A7Ibp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EGFP-C
          Sequence file: RDB15380_A7Iba.seq check
          >D04916C7_A7Ib_1_EGFP-C_D03_12_ABI24.ab1
              1 CGTTTTCTCG GCATGGACGA GCTGTACAGT CCGGACTCAG ATCCATGACT TCGGAGATGG
             61 AGTCGTCGCT GGAGGTCAGC TTCTCGTCCA GCTGTGCGGT GTCAGGGGCG TCTGGGTGTT
            121 TGCCTCCCGC CCGCTCCCGC ATCTTCAAGA TCATAGTGAT CGGCGACTCG AACGTGGGCA
            181 AGACGTGCCT GACTTACCGC TTCTGCGCCG GCCGCTTCCC CGACCGCACC GAGGCCACGA
            241 TCGGGGTGGA CTTCCGAGAG CGAGCCGTGG ATATTGATGG AGAGCGCATC AAGATCCAGT
            301 TGTGGGACAC GGCAGGGCTC GAGCGGTTCA GGAAGAGCAT GGTCCAGCAC TACTACAGGA
            361 ATGTGCATGC TGTCGTCTTT GTGTATGACA TGACCAACAT GGCCAGCTTC CACAGCCTGC
            421 CGGCTTGGAT AGAGGAATGC AAACAGCACT TGCTGGCGAA CGACATACCT CGAATTCTGG
            481 TGGGAAATAA ATGTGACTTG AGAAGCGCCA TTCAGGTGCC CACAGACTTG GCACAGAAGT
            541 TTGCTGACAC ACACAGTATG CCTTTGTTTG AGACCTCTGC TAAAAACCCC AATGATAATG
            601 ACCACGTAGA AGCTATATTC ATGACCTTAG CTCATAAGCT AAAGAGCCAC AAGCCATTGA
            661 TGCTTAGCCA GCTGCCTGAT AACAGAATTA GCCTGAAACC TGAGACCAAG CCTGCGGTGA
            721 CTTGCTGGTG CTGAAATCAC TAGTGAATTC GCGGCCGCCT GCAGGTCGAC GGTACCGCGG
            781 GCCCGGGATC CACCGGATCT AGATAACTGA TCATAATCAG CCATACCACA TTTGTAGAGG
            841 TTTTACTTGC TTTAAAAAAA CCTCCCACAC CTCCCCCCTG AAACCTGAAA CATAAAAATG
            901 AATGCAATTG TTTGTTGTTT AACTTTGTTT ATTTGCAGCT TTATAAATGG TTTACAATAA
            961 AGCAATAGCA TCACAAATTT TCACAAATAA AGCATTTTTT TTTCACTGCC ATTCCTAGCT
           1021 TGTGGATTGT CGAACTCAAT CCATGGTATC GTTAACGCGT AATTGCTAAG CGTTAAATAT
          //
          Primer: ME-1290RV
          Sequence file: RDB15380_A7Ibb.seq check
          >D04916C7_A7Ib_1_ME-1290RV_D04_10_ABI24.ab1
              1 ATCATTTCAA TTTTATGTTT CAGGTTCAGG GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT
             61 AAAACCTCTA CAAATGTGGT ATGGCTGATT ATGATCAGTT ATCTAGATCC GGTGGATCCC
            121 GGGCCCGCGG TACCGTCGAC CTGCAGGCGG CCGCGAATTC ACTAGTGATT TCAGCACCAG
            181 CAAGTCACCG CAGGCTTGGT CTCAGGTTTC AGGCTAATTC TGTTATCAGG CAGCTGGCTA
            241 AGCATCAATG GCTTGTGGCT CTTTAGCTTA TGAGCTAAGG TCATGAATAT AGCTTCTACG
            301 TGGTCATTAT CATTGGGGTT TTTAGCAGAG GTCTCAAACA AAGGCATACT GTGTGTGTCA
            361 GCAAACTTCT GTGCCAAGTC TGTGGGCACC TGAATGGCGC TTCTCAAGTC ACATTTATTT
            421 CCCACCAGAA TTCGAGGTAT GTCGTTCGCC AGCAAGTGCT GTTTGCATTC CTCTATCCAA
            481 GCCGGCAGGC TGTGGAAGCT GGCCATGTTG GTCATGTCAT ACACAAAGAC GACAGCATGC
            541 ACATTCCTGT AGTAGTGCTG GACCATGCTC TTCCTGAACC GCTCGAGCCC TGCCGTGTCC
            601 CACAACTGGA TCTTGATGCG CTCTCCATCA ATATCCACGG CTCGCTCTCG GAAGTCCACC
            661 CCGATCGTGG CCTCGGTGCG GTCGGGGAAG CGGCCGGCGC AGAAGCGGTA AGTCAGGCAC
            721 GTCTTGCCCA CGTTCGAGTC GCCGATCACT ATGATCTTGA AGATGCGGGA GCGGGCGGGA
            781 GGCAAACACC CAGACGCCCC TGACACCGCA CAGCTGGACG AGAAGCTGAC CTCCAGCGAC
            841 GACTCCATCT CCGAAGTCAT GGATCTGAGT CCGGGACTTG TACAGCTCGT CCATGCCGAG
            901 AGTGATCCCG GCGGCGGTCA CGAACTCCCA GCAGGACCAT GTGATCGCGC TTCTCGTTTG
            961 GGGGTCTTTG CTCACGCGGA CTGGGTTGCT CAGGTAGTGA TGTCCGGCCA GCCAGCACGG
          1021 GGTCGTCGAC GGAATGGGAT GATCTGCTGG TAGTGATTCG ACCGAAGCTG CA
          //
          Primer: CMV-Forward
          Sequence file: RDB15380_A7Ibc.seq check
          >D04916C7_A7Ib_1_CMV-Forward_E04_13_ABI24.ab1
              1 CGAAAACTCC GGCCCATTGA CGCAAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT
             61 AAGCAGAGCT GGTTTAGTGA ACCGTCAGAT CCGCTAGCGC TACCGGTCGC CACCATGGTG
            121 AGCAAGGGCG AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC
            181 GTAAACGGCC ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC CTACGGCAAG
            241 CTGACCCTGA AGTTCATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG
            301 ACCACCCTGA CCTACGGCGT GCAGTGCTTC AGCCGCTACC CCGACCACAT GAAGCAGCAC
            361 GACTTCTTCA AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT CTTCTTCAAG
            421 GACGACGGCA ACTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC CCTGGTGAAC
            481 CGCATCGAGC TGAAGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG GCACAAGCTG
            541 GAGTACAACT ACAACAGCCA CAACGTCTAT ATCATGGCCG ACAAGCAGAA GAACGGCATC
            601 AAGGTGAACT TCAAGATCCG CCACAACATC GAGGACGGCA GCGTGCAGCT CGCCGACCAC
            661 TACCAGCAGA ACACCCCCAT CGGCGACGGC CCCGTGCTGC TGCCCGACAA CCACTACCTG
            721 AGCACCCAGT CCGCCCTGAG CAAAGACCCC AACGAGAAGC GCGATCACAT GGTCCTGCTG
            781 GAGTTCGTGA CCGCCGCCGG GATCACTCTC GGCATGGACG AGCTGTACAA GTCCGGACTC
            841 AGATCCATGA CTTCGGAGAT GGAGGTCGTT CGCTGGGAGG GTCAGCTTCT CGTCCAGCTG
            901 TGCGGGTGTC AAGGGGCGTC TGGGGTGTTT GCTTCCCGCC CGCTTCCCGG CATCTTCAGA
            961 ATCAATAAGT GGATCGGCGA CTCGGAACCG TTGGGGCCAG GAACGTGCTG AACTTACGCT
          1021 CTGCATCAGA CGCATTTCCC TCCGCAACGG CCACCTCGGA AGGGGC
          //
          Primer: SV40pro_F_V2
          Sequence file: RDB15380_A7Ibd.seq check
          >D04916C7_A7Ib_1_SV40pro_F_V2_F04_16_ABI24.ab1
              1 GGGAGACTTT TTTGGGAGGC TAGGCTTTTG CAAGATCGAT CAAGAGACAG GATGAGGATC
             61 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
            121 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
            181 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
            241 TGAACTGCAA GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
            301 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
            361 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
            421 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
            481 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
            541 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGAGCAT
            601 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
            661 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA
            721 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA
            781 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG
            841 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGGTTC GAAATGACCG ACCAAGCGAC
            901 GCCCCAACCT GCCATCACGA GATTTCGATT CCACCGCCCG CCTTTCTATG AAAGGTTGGG
            961 GCTTTCGGAA TTCGTTTCCG GGACGGCCGG CTGGATGAAT CTTCCAGCGC CGGGGGAATC
          1021 TCATGGCTGG AGTTCTTCGT CTAATCCTTA GGGGAGCCTA ACCTGAAAAC C
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Itoh, T., Golgi-resident small GTPase Rab33B interacts with Atg16L and modulates autophagosome formation. Mol. Biol. Cell 19 (7): 2916-2925 (2008). PMID 18448665.

          2022.07.13

          GNP_filter3_RDBDEP_html_220713.pl