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pEGFP-C1-mouse Rab33B-Q92L (#RDB15380)

Expression vector of mouse RAB33B constitutive active mutant Q92L, N-terminal EGFP-tag.

Clone info. Expression vector of mouse RAB33B constitutive active mutant (GTP-hydrolysis -deficient, Q92L), fused with N-terminal EGFP, CMV promoter.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name mouse Rab33b cDNA
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Itoh, T. et al., Mol. Biol. Cell, 19 (7): 2916-2925, 2008)
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Itoh, T. et al., Mol. Biol. Cell, 19 (7): 2916-2925, 2008)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15380 pEGFP-C1-mouse Rab33B-Q92L DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-mouse Rab33B-Q92L was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15380).

Reference section:

Itoh, T., Fujita, N., Kanno, E., Yamamoto, A., Yoshimori, T., Fukuda, M., Golgi-resident small GTPase Rab33B interacts with Atg16L and modulates autophagosome formation. Mol. Biol. Cell 19 (7): 2916-2925 (2008). PMID 18448665. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15380_A7Ibp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C
Sequence file: RDB15380_A7Iba.seq check
>D04916C7_A7Ib_1_EGFP-C_D03_12_ABI24.ab1
    1 CGTTTTCTCG GCATGGACGA GCTGTACAGT CCGGACTCAG ATCCATGACT TCGGAGATGG
   61 AGTCGTCGCT GGAGGTCAGC TTCTCGTCCA GCTGTGCGGT GTCAGGGGCG TCTGGGTGTT
  121 TGCCTCCCGC CCGCTCCCGC ATCTTCAAGA TCATAGTGAT CGGCGACTCG AACGTGGGCA
  181 AGACGTGCCT GACTTACCGC TTCTGCGCCG GCCGCTTCCC CGACCGCACC GAGGCCACGA
  241 TCGGGGTGGA CTTCCGAGAG CGAGCCGTGG ATATTGATGG AGAGCGCATC AAGATCCAGT
  301 TGTGGGACAC GGCAGGGCTC GAGCGGTTCA GGAAGAGCAT GGTCCAGCAC TACTACAGGA
  361 ATGTGCATGC TGTCGTCTTT GTGTATGACA TGACCAACAT GGCCAGCTTC CACAGCCTGC
  421 CGGCTTGGAT AGAGGAATGC AAACAGCACT TGCTGGCGAA CGACATACCT CGAATTCTGG
  481 TGGGAAATAA ATGTGACTTG AGAAGCGCCA TTCAGGTGCC CACAGACTTG GCACAGAAGT
  541 TTGCTGACAC ACACAGTATG CCTTTGTTTG AGACCTCTGC TAAAAACCCC AATGATAATG
  601 ACCACGTAGA AGCTATATTC ATGACCTTAG CTCATAAGCT AAAGAGCCAC AAGCCATTGA
  661 TGCTTAGCCA GCTGCCTGAT AACAGAATTA GCCTGAAACC TGAGACCAAG CCTGCGGTGA
  721 CTTGCTGGTG CTGAAATCAC TAGTGAATTC GCGGCCGCCT GCAGGTCGAC GGTACCGCGG
  781 GCCCGGGATC CACCGGATCT AGATAACTGA TCATAATCAG CCATACCACA TTTGTAGAGG
  841 TTTTACTTGC TTTAAAAAAA CCTCCCACAC CTCCCCCCTG AAACCTGAAA CATAAAAATG
  901 AATGCAATTG TTTGTTGTTT AACTTTGTTT ATTTGCAGCT TTATAAATGG TTTACAATAA
  961 AGCAATAGCA TCACAAATTT TCACAAATAA AGCATTTTTT TTTCACTGCC ATTCCTAGCT
 1021 TGTGGATTGT CGAACTCAAT CCATGGTATC GTTAACGCGT AATTGCTAAG CGTTAAATAT
//
Primer: ME-1290RV
Sequence file: RDB15380_A7Ibb.seq check
>D04916C7_A7Ib_1_ME-1290RV_D04_10_ABI24.ab1
    1 ATCATTTCAA TTTTATGTTT CAGGTTCAGG GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT
   61 AAAACCTCTA CAAATGTGGT ATGGCTGATT ATGATCAGTT ATCTAGATCC GGTGGATCCC
  121 GGGCCCGCGG TACCGTCGAC CTGCAGGCGG CCGCGAATTC ACTAGTGATT TCAGCACCAG
  181 CAAGTCACCG CAGGCTTGGT CTCAGGTTTC AGGCTAATTC TGTTATCAGG CAGCTGGCTA
  241 AGCATCAATG GCTTGTGGCT CTTTAGCTTA TGAGCTAAGG TCATGAATAT AGCTTCTACG
  301 TGGTCATTAT CATTGGGGTT TTTAGCAGAG GTCTCAAACA AAGGCATACT GTGTGTGTCA
  361 GCAAACTTCT GTGCCAAGTC TGTGGGCACC TGAATGGCGC TTCTCAAGTC ACATTTATTT
  421 CCCACCAGAA TTCGAGGTAT GTCGTTCGCC AGCAAGTGCT GTTTGCATTC CTCTATCCAA
  481 GCCGGCAGGC TGTGGAAGCT GGCCATGTTG GTCATGTCAT ACACAAAGAC GACAGCATGC
  541 ACATTCCTGT AGTAGTGCTG GACCATGCTC TTCCTGAACC GCTCGAGCCC TGCCGTGTCC
  601 CACAACTGGA TCTTGATGCG CTCTCCATCA ATATCCACGG CTCGCTCTCG GAAGTCCACC
  661 CCGATCGTGG CCTCGGTGCG GTCGGGGAAG CGGCCGGCGC AGAAGCGGTA AGTCAGGCAC
  721 GTCTTGCCCA CGTTCGAGTC GCCGATCACT ATGATCTTGA AGATGCGGGA GCGGGCGGGA
  781 GGCAAACACC CAGACGCCCC TGACACCGCA CAGCTGGACG AGAAGCTGAC CTCCAGCGAC
  841 GACTCCATCT CCGAAGTCAT GGATCTGAGT CCGGGACTTG TACAGCTCGT CCATGCCGAG
  901 AGTGATCCCG GCGGCGGTCA CGAACTCCCA GCAGGACCAT GTGATCGCGC TTCTCGTTTG
  961 GGGGTCTTTG CTCACGCGGA CTGGGTTGCT CAGGTAGTGA TGTCCGGCCA GCCAGCACGG
1021 GGTCGTCGAC GGAATGGGAT GATCTGCTGG TAGTGATTCG ACCGAAGCTG CA
//
Primer: CMV-Forward
Sequence file: RDB15380_A7Ibc.seq check
>D04916C7_A7Ib_1_CMV-Forward_E04_13_ABI24.ab1
    1 CGAAAACTCC GGCCCATTGA CGCAAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT
   61 AAGCAGAGCT GGTTTAGTGA ACCGTCAGAT CCGCTAGCGC TACCGGTCGC CACCATGGTG
  121 AGCAAGGGCG AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC
  181 GTAAACGGCC ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC CTACGGCAAG
  241 CTGACCCTGA AGTTCATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG
  301 ACCACCCTGA CCTACGGCGT GCAGTGCTTC AGCCGCTACC CCGACCACAT GAAGCAGCAC
  361 GACTTCTTCA AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT CTTCTTCAAG
  421 GACGACGGCA ACTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC CCTGGTGAAC
  481 CGCATCGAGC TGAAGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG GCACAAGCTG
  541 GAGTACAACT ACAACAGCCA CAACGTCTAT ATCATGGCCG ACAAGCAGAA GAACGGCATC
  601 AAGGTGAACT TCAAGATCCG CCACAACATC GAGGACGGCA GCGTGCAGCT CGCCGACCAC
  661 TACCAGCAGA ACACCCCCAT CGGCGACGGC CCCGTGCTGC TGCCCGACAA CCACTACCTG
  721 AGCACCCAGT CCGCCCTGAG CAAAGACCCC AACGAGAAGC GCGATCACAT GGTCCTGCTG
  781 GAGTTCGTGA CCGCCGCCGG GATCACTCTC GGCATGGACG AGCTGTACAA GTCCGGACTC
  841 AGATCCATGA CTTCGGAGAT GGAGGTCGTT CGCTGGGAGG GTCAGCTTCT CGTCCAGCTG
  901 TGCGGGTGTC AAGGGGCGTC TGGGGTGTTT GCTTCCCGCC CGCTTCCCGG CATCTTCAGA
  961 ATCAATAAGT GGATCGGCGA CTCGGAACCG TTGGGGCCAG GAACGTGCTG AACTTACGCT
1021 CTGCATCAGA CGCATTTCCC TCCGCAACGG CCACCTCGGA AGGGGC
//
Primer: SV40pro_F_V2
Sequence file: RDB15380_A7Ibd.seq check
>D04916C7_A7Ib_1_SV40pro_F_V2_F04_16_ABI24.ab1
    1 GGGAGACTTT TTTGGGAGGC TAGGCTTTTG CAAGATCGAT CAAGAGACAG GATGAGGATC
   61 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
  121 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
  181 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
  241 TGAACTGCAA GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
  301 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
  361 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
  421 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
  481 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
  541 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGAGCAT
  601 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
  661 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA
  721 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA
  781 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG
  841 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGGTTC GAAATGACCG ACCAAGCGAC
  901 GCCCCAACCT GCCATCACGA GATTTCGATT CCACCGCCCG CCTTTCTATG AAAGGTTGGG
  961 GCTTTCGGAA TTCGTTTCCG GGACGGCCGG CTGGATGAAT CTTCCAGCGC CGGGGGAATC
1021 TCATGGCTGG AGTTCTTCGT CTAATCCTTA GGGGAGCCTA ACCTGAAAAC C
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Itoh, T., Golgi-resident small GTPase Rab33B interacts with Atg16L and modulates autophagosome formation. Mol. Biol. Cell 19 (7): 2916-2925 (2008). PMID 18448665. [link to RRC of NBRP]