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pEGFP-C1-human DENN

Expression vector of human DENN (MADD).

Catalog number RDB15288
Resource name pEGFP-C1-human DENN
Clone info. Expression vector of human DENN [MADD in NCBI], fused with N-terminal EGFP, CMV promoter.
Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells)
Gene/insert name human MADD cDNA
Depositor|Developer Fukuda, Mitsunori |
 
Other clones in our bank human MADD (NCBI Gene 8567) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Imai, A. et al., Arch. Biochem. Biophys., 536 (1): 31-37, 2013).
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Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Schedule A (GFP-A) [Link]
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15288 pEGFP-C1-human DENN DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Imai, A., MADD/DENN/Rab3GEP functions as a guanine nucleotide exchange factor for Rab27 during granule exocytosis of rat parotid acinar cells. Arch. Biochem. Biophys. 536 (1): 31-37 (2013). PMID 23702376.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15288_A7Gop1_2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2
Sequence file: RDB15288_A7Goa.seq check
>D04773A6_A7Go_2_EGFP-C2_E07_13_ABI24.ab1
    1 GACCGAACGA GAGCGCGATC ACATGGTCCT GCTGGAGTTC GTGACCGCCG CCGGGATCAC
   61 TCTCGGCATG GACGAGCTGT ACAAGTCCGG ACTCAGATCT ATGGTGCAAA AGAAGAAGTT
  121 CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC AGGCACCCGA GCAGTGATAG
  181 CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG GAGGATCACA CTGAGTTTCC
  241 CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC TGCCTGAGCG TGCGGCAGCG
  301 GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC CTCACTGACA AGGACACTGG
  361 AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC TTCCAAAAGC GAATCTCTAA
  421 GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA GGAACCCATG CCACCTGTGC
  481 CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA TCCCTGCAGC CTCTCAGTGC
  541 TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA CGCCGGGCCA AGGCGGGGAG
  601 CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC AGCCACTACC CTTTCTTCTC
  661 CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG GACTGCTGTA GTGAGCGCCT
  721 TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG GACACCATGT GGCGGATCTT
  781 TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT CTGCATGACC TTCGAGAGAT
  841 TGAGGCCTGT ATCTATCGAT TGCTGCGCTC CCCAGTACCC GTCTCTGGGC AGAAGCGAGT
  901 AGACATCGAG GGTCCTACCC CAAGAGCTCC AGCCAGCTCT GACCTTTGCT CTTCCAGACC
  961 CATCTCGATT CACCCTAGTG GATTTCCCAC TGCACCTTCC CTTGACTTCT AGTGTGACGC
 1021 CTGTCTCCAG TGCTAACTTG CAATCCTGGT AGAGCACAGG TTGAGCTACA GTCCGAGAAC
1081 TACATGCACT CCTCCATGGT CCT
//
Primer: pAxEF_R2
Sequence file: RDB15288_A7Gob.seq check
>D04773A6_A7Go_2_pAxEF_R2_F07_16_ABI24.ab1
    1 CAGTCAAAAT GCTTTATTTG TGAATTTGTG ATGCTATTGC TTTATTTGTA ACCATTATAA
   61 GCTGCAATAA ACAAGTTAAC AACAACAATT GCATTCATTT TATGTTTCAG GTTCAGGGGG
  121 AGGTGTGGGA GGTTTTTTAA AGCAAGTAAA ACCTCTACAA ATGTGGTATG GCTGATTATG
  181 ATCAGTTATC TAGATCCGGT GGATCCCGGG CCCGCGGTAC CGTCGACTGC AGAATTCGAA
  241 GCTTGAGCTC GAGATCTTCC TCACTGCTAT GAACTGCAGC CACGTACGAG AAGAGACATA
  301 ATACGGAGTA GCAGATTTCG TGGGCCATTG GTGTCTTGTA CTTGTGGCTC ACCACTTCTT
  361 TAATTTCAGG AACTGATTGT GCATGAATTT GAGGTTGATC CCTTCCAGGG TCACCTGCAG
  421 CAGGCCACCC TCACCAGTCT TCAGGTCCTG CACAGGGAAC TCGCCACCCA ATTCAGGTCC
  481 GGGTTTGATG CTTTGCTGTC GGGCGGCAGC GCGCTCCATG CTGTCCTTCA CACAGTAGTA
  541 CAGCTCCCGA CACTTTTTAG TATAGAACTT GTTGAGCTGG GTCTCAGAGC CATTTCTACG
  601 CCACAAGCAC AACACCTTCG TCTTGGGACA GTAGGTCATG TTGATGAGCT TCTCGTACCA
  661 CCAGCGCTCA TACACTGTTC CGATGTTACT ACGCAACACC ACACAGTCAT CGCACACCTC
  721 CATGAAAAAG ATATCTCCGT TTGTATCTGT CCCTGCATGT ACCACAAATG TCTGCTTCTT
  781 CATGTGCCGG CTGCCACTGG ACCAGATAGA GAGATCGCGT CCATTCACGT TCGCCAGCTG
  841 ATCAAGCACC TCATTGATTT GCTGGCTGTA CACAAGCCCA ATGTGCGACT TTCCCATTAG
  901 CGCCTCACCT TCTTGCGGAT GTCATTCTTA TTTACCTTCA TCAGCAGCAT GTAGAGATGA
  961 GGTTGTGCAG AAGTGTGGCC AGCAAGCGAT CTTCATCATC TCCAGGCGCT TTCCGGTCAT
 1021 GTCTCAAGGA CAGGTACTGT CGATCATTTC TGGGGACCCT GATCATACCA ATCCATCTCT
1081 CTCCAACATT CACAAGCCA
//
Primer: CMV-Forward
Sequence file: RDB15288_A7Goc.seq check
>D04773A6_A7Go_2_CMV-Forward_G07_19_ABI24.ab1
    1 CGTCCTCCGC CCATTGACGC AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC
   61 AGAGCTGGTT TAGTGAACCG TCAGATCCGC TAGCGCTACC GGTCGCCACC ATGGTGAGCA
  121 AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA
  181 ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA
  241 CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA
  301 CCCTGACCTA CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT
  361 TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG
  421 ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA
  481 TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT
  541 ACAACTACAA CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC GGCATCAAGG
  601 TGAACTTCAA GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC
  661 AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCA
  721 CCCAGTCCGC CCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT
  781 TCGTGACCGC CGCCGGGATC ACTCTCGGCA TGGACGAGCT GTACAAGTCC GGACTCAGAT
  841 CTATGGTGCA AAAGAAGAAG TTCTGTCCTC GGGTTACTTG ACTATCTAGT GATCGTAGGG
  901 CCCAGGGCAC CCGAGCAGTG ATAGCGTGGC CCAGACTCCT GAATTGCTAC GGCGATACCC
  961 CTTGTAGGAT CACACTGAGT TCCCCTGCCC CCAGATGTAG TGTCTCCTGG CAAGCCGAGG
 1021 GCTGCTGAGC GTGCGCAGCG ACGCAATGAT CCTTCGATGG ATACATCTTG CTTCACCGTT
1081 CAATTGACAG A
//
Primer: SV40pro_F_V2
Sequence file: RDB15288_A7God.seq check
>D04773A6_A7Go_2_SV40pro_F_V2_A10_01_ABI24.ab1
    1 ACCAGGCTTT TTTTGGAGGC TAGGCTTTTG CAAGATCGAT CAAGAGACAG GATGAGGATC
   61 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
  121 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
  181 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
  241 TGAACTGCAA GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
  301 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
  361 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
  421 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
  481 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
  541 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGAGCAT
  601 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
  661 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA
  721 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA
  781 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG
  841 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG
  901 CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTTATGAAA GGTTGGGCTT
  961 CGGAATCGTT TTTCCGGACG CCGGCTGGAT GATCCTCCAG CGGCGGGGGA TCCTCATGCT
 1021 GAGTTCTTCG CCCACCTAGG GGGGAGGCCT ACCTGGAAAC ACGGAAAGGG AGACAATACC
 1081 GGAAGACCCG CGCCTTATTG ACCGCAAATA AAGACCGATA ACGGCAACGG TTTGGTTCGG
1141 TTTGTTTCTC ATAAACGTCG GGGGGT
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Imai, A., MADD/DENN/Rab3GEP functions as a guanine nucleotide exchange factor for Rab27 during granule exocytosis of rat parotid acinar cells. Arch. Biochem. Biophys. 536 (1): 31-37 (2013). PMID 23702376.

2021.03.25

GNP_filter3_RDBDEP_html_210308.pl