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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEF-FLAG-mouse myosin VIIa-tail

Expression vector of mouse myosin VIIa tail region (Myo7a).

Catalog number RDB15287
Resource name pEF-FLAG-mouse myosin VIIa-tail
Clone info. Expression vector of mouse myosin VIIa tail region [Myo7a in NCBI], tagged with N-terminal FLAG, EF1 promoter. ｃDNA fragment 2837nt to 6908nt of NM_001256081.1 is cloned.
Comment Expression was confirmed by the depositor with immunoblotting. Known differences (at least) : eight synonymus substitutions compared with NM_001256081.1, T to C at 2880nt, G to A at 2996nt, T to C at 3485nt, C to T at 3509nt, A to G at 6388nt, T to C at 6635nt, C to T at 6899nt, G to A at 6902nt.
Vector backbone pEF-BOS FLAG (plasmid)
Size of vector backbone 5.7 kb
Selectable markers Amp^r
Gene/insert name mouse Myo7a cDNA
Depositor|Developer Fukuda, Mitsunori |
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External Database
mouse Myo7a
          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Fukuda, M. and Kuroda T. S., J. Biol. Chem., 277 (45): 43096-43103, 2002).
          Additional terms and conditions for distribution Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Fukuda, M. and Kuroda T. S., J. Biol. Chem., 277 (45): 43096-43103, 2002)。
          付加的提供条件 pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB15287 pEF-FLAG-mouse myosin VIIa-tail DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Fukuda, M., Slac2-c (synaptotagmin-like protein homologue lacking C2 domains-c), a novel linker protein that interacts with Rab27, myosin Va/VIIa, and actin. J. Biol. Chem. 277 (45): 43096-43103 (2002). PMID 12221080.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15287_A7Gop1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EF1a-C_F
          Sequence file: RDB15287_A7Goa.seq check
          >D04773A5_A7Go_2_EF1a-C_F_A06_01_ABI08.ab1
              1 CCGGGGATTG CCCTTTTTGA GTTTGGATCT TGGTTCATTC TCAAGCCTCA GACAGTGGTT
             61 CAAAGTTTTT TTCTTCCATT TCAGGTGTCG TGAGGAATTC TCTAGAGCGG CCGCGGGAAT
            121 TCGATTCCAC CATGGACTAC AAGGACGATG ACGACAAGGG ATCCCGGGTT GAGTACCAGC
            181 GGCGCCTCGA GGCAGAGAGG ATGCGTTTGG CAGAGGAGGA GAAACTCCGA AAGGAGATGA
            241 GTGCCAAGAA GGCCAAAGAG GAGGCTGAGC GCAAGCATCA GGAGCGCCTG GCTCAGCTAG
            301 CCCGCGAGGA TGCGGAGCGG GAGCTGAAGG AGAAGGAGGA GGCTCGGAGG AAGAAGGAAC
            361 TGCTGGAGCA GATGGAGAAG GCCCGCCACG AACCCATCAA CCACTCAGAT ATGGTGGACA
            421 AGATGTTTGG CTTCCTGGGG ACTTCAGGCA GCCTGCCAGG CCAGGAAGGC CAGGCGCCTA
            481 GTGGCTTTGA GGACCTAGAG CGCGGACGGA GGGAGATGGT GGAAGAGGAT GTTGACGCTG
            541 CCCTGCCCCT GCCTGATGAA GACGAGGAGG ACCTTTCTGA GTACAAATTC GCCAAGTTTG
            601 CTGCCACCTA CTTCCAGGGC ACAACCACAC ACTCCTACAC CCGGAGGCCT CTCAAGCAGC
            661 CGCTGCTCTA CCACGACGAT GAGGGTGACC AGCTGGCGGC GCTGGCTGTC TGGATCACCA
            721 TCCTCCGGTT CATGGGGGAC CTCCCAGAGC CCAAGTACCA CACAGCCATG AGCGACGGCA
            781 GTGAGAAGAT CCCAGTGATG ACTAAGATCT ATGAGACCCT AGGCAAGAAG ACATACAAGA
            841 GGGAGCTGCA GCCTTGCAGG GCGAGGGCGA GACCCAGCTC CCTGAGGGGC AGAAGAAGAC
            901 CAGTGTGAGA CACAAGTTGG TACACTTGAC ACTGAAGAAG AAGTCCCAAC TCACAGAAGA
            961 GGTGACCAAA GAGGCTGAAC GATGGGGGAA TCCACGGGTA CAGGTACAGC ATGGCTGTAG
           1021 GATCGGGCCC ACCTCAAATC TAGAGAAGCT TGCACTTCAT TCATCGGCAC GGCAATTCCT
          1081 GACAGATCCT GTCGCTC
          //
          Primer: hCGSF_R
          Sequence file: RDB15287_A7Gob.seq check
          >D04773A5_A7Go_2_hGCSF_R_E10_13_ABI24.ab1
              1 GGGGGGACCT GATCTCTCAG GGTCCCAGGT GGGGACCCTC ACTCTAGAGC GGCCGCTATG
             61 GTCGACGGTA TCGATAAGCT TGATATCGAA TTCGATTCTC CTCTGCGGTT CACCTCCCGC
            121 TCCTGGAGTT CCTCTGCTTG CTCATGGCTG TGAGCATCTG GCTGATGTAG GAAGTCAGAA
            181 GATCATCCAT TTTGTATCCC AGCGATGTCT CACAGAGCAG TTTGCTCCCA CGGACCAAGT
            241 TCCCAATGGT GATGTGGAAG TAGGTGTTGC CACTACTCCA GTTGGAGATC TTGGTGAAGG
            301 GGTGAGTAGT CAGGATGTCC TTGGTTCTGG GATCGATGAG GCTGACCCCG TACTTGTTGA
            361 TGGCAATTAA GAGAATCTCT GGAAAGTTTG GTTCTGTAGT TTGCTTCACC TCAAAGAAGG
            421 CTGAGCCAAA GGTGGGCCAC TTGAAGATGA GTTTGAGGAA GGCCAGCTTG GCTTCCTCCT
            481 TGGACTTCCC CGCATGTTTG TTGAAGTAGG CGACAATAGA CCGTTTCCAG TCATCAGGTG
            541 AGACCTGCCG GATTAGATCC TGGGGTACCA GCTCCCTCAG CAACTTGGGG ATGCTAGGGA
            601 AGTAGGATTT GTCCTCCTCA AACTTGACTC TGTAGATGAG TGCGCCCAGC TGCAGCACCT
            661 CCTCCCGGGT GCACTTGTGG TAGCCTCGGA GATATTTGGG CAGTTCCTGG TAATAGTGGA
            721 AGATGGAGTC AGCCATGGGG TCCTTGCCCG GCACTGTGGT GGTCCACAGC TTCTTCATGA
            781 AGAACACCTG ATAGGTTAGT GAGGGCACGA TTCCGTCCTT GATGGGCCGT GCTTTCTTTA
            841 TCCAGTCTGT CAGTGTCGGA CAAAGTCAAA GAAGAATCAT TCTCTGGGAC GCTGATGACC
            901 TTATCTGCGA TTTTGACAAA AGCTGATCCC TCGGAGACTT GAGCAGCAGC CGGCTGGCGA
            961 TGTCTGGCAG AGTCCTTGAC TTGTGCTGAC TCCACTCAAG CCTCGTCGTG TCATCGGGAG
           1021 TAGACTGTGA GATCTGGTAG TCTTATGTGA TGCTTCACCT CACAGGTGCG AAGGTACCTC
          1081 GAGATCATTC TCAGCATTCT GACTCTGCAG GCCAGTTCGA TGCCAGAGAC CG
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Fukuda, M., Slac2-c (synaptotagmin-like protein homologue lacking C2 domains-c), a novel linker protein that interacts with Rab27, myosin Va/VIIa, and actin. J. Biol. Chem. 277 (45): 43096-43103 (2002). PMID 12221080.

          2022.05.17

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