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pEF-FLAG-mouse myosin VIIa-tail (#RDB15287)

Expression vector of mouse myosin VIIa tail region (Myo7a).

Clone info. Expression vector of mouse myosin VIIa tail region [Myo7a in NCBI], tagged with N-terminal FLAG, EF1 promoter. ｃDNA fragment 2837nt to 6908nt of NM_001256081.1 is cloned.
Comment Expression was confirmed by the depositor with immunoblotting. Known differences (at least) : eight synonymus substitutions compared with NM_001256081.1, T to C at 2880nt, G to A at 2996nt, T to C at 3485nt, C to T at 3509nt, A to G at 6388nt, T to C at 6635nt, C to T at 6899nt, G to A at 6902nt.
Vector backbone pEF-BOS FLAG (plasmid)
Size of vector backbone 5.7 kb
Selectable markers Amp^r
Gene/insert name mouse Myo7a cDNA
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Fukuda, M. and Kuroda T. S., J. Biol. Chem., 277 (45): 43096-43103, 2002).
Additional terms and conditions:
Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Fukuda, M. and Kuroda T. S., J. Biol. Chem., 277 (45): 43096-43103, 2002)。
MTAに書く付加的使用条件：
pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15287 pEF-FLAG-mouse myosin VIIa-tail DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEF-FLAG-mouse myosin VIIa-tail was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15287).

Reference section:

Fukuda, M., Kuroda, T.S., Slac2-c (synaptotagmin-like protein homologue lacking C2 domains-c), a novel linker protein that interacts with Rab27, myosin Va/VIIa, and actin. J. Biol. Chem. 277 (45): 43096-43103 (2002). PMID 12221080. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15287_A7Gop1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F
Sequence file: RDB15287_A7Goa.seq check
>D04773A5_A7Go_2_EF1a-C_F_A06_01_ABI08.ab1
    1 CCGGGGATTG CCCTTTTTGA GTTTGGATCT TGGTTCATTC TCAAGCCTCA GACAGTGGTT
   61 CAAAGTTTTT TTCTTCCATT TCAGGTGTCG TGAGGAATTC TCTAGAGCGG CCGCGGGAAT
  121 TCGATTCCAC CATGGACTAC AAGGACGATG ACGACAAGGG ATCCCGGGTT GAGTACCAGC
  181 GGCGCCTCGA GGCAGAGAGG ATGCGTTTGG CAGAGGAGGA GAAACTCCGA AAGGAGATGA
  241 GTGCCAAGAA GGCCAAAGAG GAGGCTGAGC GCAAGCATCA GGAGCGCCTG GCTCAGCTAG
  301 CCCGCGAGGA TGCGGAGCGG GAGCTGAAGG AGAAGGAGGA GGCTCGGAGG AAGAAGGAAC
  361 TGCTGGAGCA GATGGAGAAG GCCCGCCACG AACCCATCAA CCACTCAGAT ATGGTGGACA
  421 AGATGTTTGG CTTCCTGGGG ACTTCAGGCA GCCTGCCAGG CCAGGAAGGC CAGGCGCCTA
  481 GTGGCTTTGA GGACCTAGAG CGCGGACGGA GGGAGATGGT GGAAGAGGAT GTTGACGCTG
  541 CCCTGCCCCT GCCTGATGAA GACGAGGAGG ACCTTTCTGA GTACAAATTC GCCAAGTTTG
  601 CTGCCACCTA CTTCCAGGGC ACAACCACAC ACTCCTACAC CCGGAGGCCT CTCAAGCAGC
  661 CGCTGCTCTA CCACGACGAT GAGGGTGACC AGCTGGCGGC GCTGGCTGTC TGGATCACCA
  721 TCCTCCGGTT CATGGGGGAC CTCCCAGAGC CCAAGTACCA CACAGCCATG AGCGACGGCA
  781 GTGAGAAGAT CCCAGTGATG ACTAAGATCT ATGAGACCCT AGGCAAGAAG ACATACAAGA
  841 GGGAGCTGCA GCCTTGCAGG GCGAGGGCGA GACCCAGCTC CCTGAGGGGC AGAAGAAGAC
  901 CAGTGTGAGA CACAAGTTGG TACACTTGAC ACTGAAGAAG AAGTCCCAAC TCACAGAAGA
  961 GGTGACCAAA GAGGCTGAAC GATGGGGGAA TCCACGGGTA CAGGTACAGC ATGGCTGTAG
 1021 GATCGGGCCC ACCTCAAATC TAGAGAAGCT TGCACTTCAT TCATCGGCAC GGCAATTCCT
1081 GACAGATCCT GTCGCTC
//
Primer: hCGSF_R
Sequence file: RDB15287_A7Gob.seq check
>D04773A5_A7Go_2_hGCSF_R_E10_13_ABI24.ab1
    1 GGGGGGACCT GATCTCTCAG GGTCCCAGGT GGGGACCCTC ACTCTAGAGC GGCCGCTATG
   61 GTCGACGGTA TCGATAAGCT TGATATCGAA TTCGATTCTC CTCTGCGGTT CACCTCCCGC
  121 TCCTGGAGTT CCTCTGCTTG CTCATGGCTG TGAGCATCTG GCTGATGTAG GAAGTCAGAA
  181 GATCATCCAT TTTGTATCCC AGCGATGTCT CACAGAGCAG TTTGCTCCCA CGGACCAAGT
  241 TCCCAATGGT GATGTGGAAG TAGGTGTTGC CACTACTCCA GTTGGAGATC TTGGTGAAGG
  301 GGTGAGTAGT CAGGATGTCC TTGGTTCTGG GATCGATGAG GCTGACCCCG TACTTGTTGA
  361 TGGCAATTAA GAGAATCTCT GGAAAGTTTG GTTCTGTAGT TTGCTTCACC TCAAAGAAGG
  421 CTGAGCCAAA GGTGGGCCAC TTGAAGATGA GTTTGAGGAA GGCCAGCTTG GCTTCCTCCT
  481 TGGACTTCCC CGCATGTTTG TTGAAGTAGG CGACAATAGA CCGTTTCCAG TCATCAGGTG
  541 AGACCTGCCG GATTAGATCC TGGGGTACCA GCTCCCTCAG CAACTTGGGG ATGCTAGGGA
  601 AGTAGGATTT GTCCTCCTCA AACTTGACTC TGTAGATGAG TGCGCCCAGC TGCAGCACCT
  661 CCTCCCGGGT GCACTTGTGG TAGCCTCGGA GATATTTGGG CAGTTCCTGG TAATAGTGGA
  721 AGATGGAGTC AGCCATGGGG TCCTTGCCCG GCACTGTGGT GGTCCACAGC TTCTTCATGA
  781 AGAACACCTG ATAGGTTAGT GAGGGCACGA TTCCGTCCTT GATGGGCCGT GCTTTCTTTA
  841 TCCAGTCTGT CAGTGTCGGA CAAAGTCAAA GAAGAATCAT TCTCTGGGAC GCTGATGACC
  901 TTATCTGCGA TTTTGACAAA AGCTGATCCC TCGGAGACTT GAGCAGCAGC CGGCTGGCGA
  961 TGTCTGGCAG AGTCCTTGAC TTGTGCTGAC TCCACTCAAG CCTCGTCGTG TCATCGGGAG
 1021 TAGACTGTGA GATCTGGTAG TCTTATGTGA TGCTTCACCT CACAGGTGCG AAGGTACCTC
1081 GAGATCATTC TCAGCATTCT GACTCTGCAG GCCAGTTCGA TGCCAGAGAC CG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Fukuda, M., Slac2-c (synaptotagmin-like protein homologue lacking C2 domains-c), a novel linker protein that interacts with Rab27, myosin Va/VIIa, and actin. J. Biol. Chem. 277 (45): 43096-43103 (2002). PMID 12221080. [link to RRC of NBRP]