RDB15284 - pEF-FLAG-mouse myosin Va BR-tail (Brain type)

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icon below as well as clone information before placing your order.

pEF-FLAG-mouse myosin Va BR-tail (Brain type) (#RDB15284)

Expression vector of mouse brain-type myosin Va tail region (+ exon B, - exon D and F).

Clone info.	Expression vector of mouse brain (BR)-type myosin Va tail region (+ exon B, - exon D and F), tagged with N-terminal FLAG, EF1 promoter. ｃDNA fragment 3161nt to 5989nt of NM_010864.2 is cloned.
Comment	Expression was confirmed by the depositor with immunoblotting. Known differences (at least) : G to A substitution at 3481nt of NM_010864.2 (Gln, Synonym).
Vector backbone	pEF-BOS FLAG (plasmid)
Size of vector backbone	5.7 kb
Selectable markers	Amp^r
Gene/insert name	mouse Myo5a cDNA
Depositor\|Developer	Fukuda, Mitsunori \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Fukuda, M. and Kuroda T. S., J. Biol. Chem., 277 (45): 43096-43103, 2002).
Additional terms and conditions:
Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Fukuda, M. and Kuroda T. S., J. Biol. Chem., 277 (45): 43096-43103, 2002). Additional terms and conditions: Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Fukuda, M. and Kuroda T. S., J. Biol. Chem., 277 (45): 43096-43103, 2002)。 MTAに書く付加的使用条件： pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。

必要書類

提供依頼書

[依頼書の記入例

]
提供同意書 (MTA, 非営利学術目的用)[Word

] [MTAの記入例

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。

MTAに書く使用条件

利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Fukuda, M. and Kuroda T. S., J. Biol. Chem., 277 (45): 43096-43103, 2002)。
MTAに書く付加的使用条件：
pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15284	pEF-FLAG-mouse myosin Va BR-tail (Brain type)	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEF-FLAG-mouse myosin Va BR-tail (Brain type) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15284).

Reference section:

Fukuda, M., Kuroda, T.S., Slac2-c (synaptotagmin-like protein homologue lacking C2 domains-c), a novel linker protein that interacts with Rab27, myosin Va/VIIa, and actin. J. Biol. Chem. 277 (45): 43096-43103 (2002). PMID 12221080. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB15284_A7Gop1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F Sequence file: RDB15284_A7Goa.seq
>D04773A2_A7Go_2_EF1a-C_F_B07_04_ABI24.ab1 1 TAAGGCCATT GCTTTTTGAG TTTGGATCTT GGTTCATTCT CAAGCCTCAG ACAGTGGTTC 61 AAAGTTTTTT TCTTCCATTT CAGGTGTCGT GAGGAATTCT CTAGAGCGGC CGCGGGAATT 121 CGATTCCACC ATGGACTACA AGGACGATGA CGACAAGGGA TCCGCTCGCT CTGTGGAACG 181 CTACAAGAAG CTCCATATTG GCATGGAGAA CAAGATTATG CAGCTGCAGC GCAAAGTGGA 241 TGAGCAGAAT AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTGG AAGGAGTATA 301 CAACTCTGAG ACTGAAAAAC TACGAAATGA TGTAGAACGT CTTCAGCTAA GTGAAGAGGA 361 AGCTAAGGTT GCCACTGGGC GGGTCCTTAG TCTGCAGGAA GAAATTGCAA AACTCCGAAA 421 AGACCTGGAA CAAACTCGAT CAGAGAAAAA GTCTATTGAA GAAAGAGCAG ATAAATACAA 481 ACAGGAAACT GACCAGCTGG TGTCAAACTT GAAGGAAGAA AATACTTTGC TGAAGCAGGA 541 AAAGGAGACC CTCAACCACC GCATTGTGGA GCAGGCGAAG GAGATGACAG AAACTATGGA 601 GAGGAAGTTA GTAGAAGAGA CAAAACAACT GGAGCTTGAC CTGAATGATG AGAGGCTGAG 661 GTATCAGAAC CTTCTGAATG AGTTCAGTCG CCTGGAGGAG CGCTATGATG ACCTCAAGGA 721 AGAGATGACC CTGATGCTGA ATGTGCCTAA GCCAGGACAC AAGAGAACAG ACTCTACCCA 781 CAGCAGTAAT GAGTCTGAAT ACACCTTCAG CTCTGAGTTT GCAGAAACTG AAGACATTGC 841 ACCAAGAACA GAGGAGCCAA TTGAGAAGAA GGTGCCTCTG GATATGTCAC TATTCCTTAA 901 GCTCCAGAAG CGTGTCACAG AGCTGGGAAC AGGAGAAGCA GCTGATGCAG GATGAGCTGG 961 GACCGCAGGA GGAAGCAGTT GTTTCCGCAG CAGGCAAAGA GAGAATGGGC ACAGATAGAG 1021 AGCTGACTAG AGTATGAGTC TCTCAGCGTC AGACTTGCAG TCCGTAACCG AAAAACTGAA 1081 AAACCGAGCC TG //
Primer: hCGSF_R Sequence file: RDB15284_A7Gob.seq
>D04773A2_A7Go_2_hGCSF_R_B08_05_ABI24.ab1 1 CTGGACTGAT CTCTCAGGGT CCAGGTGGGG ACCCTCACTC TAGAGCGGCC GCTATGGTCG 61 ACGGTATCGA TAAGCTTGAT ATCGAATTCG ATTCAGACCC GTGCGATGAA GCCCAGGCCC 121 AGGCTGGCTG GGATCTGGAT GGTTTCTAGG GCTAGGGAGG ATGGGTTAAA GGGAAAAGTG 181 ACAGGAAAGA TGTGTTTTGC ATCCATGAGC AGCTGTGGAG AGTCTTTCCT GTCTCGTAAC 241 CGCATCTGTA TAGTGCGGAT AAATGAAACT GAGACCCTTT CTTCAAACTC ATTAACCGGT 301 GTGTACAGAT TCAACACTTT GACAATCTGG GCCGTGGTCA GGGCGTTGCA CATGGAACAG 361 ATGGCTTCTG CGTCATCGTC AGTTTTCTTT TTCACTTGCA AAAGCTGAGC GGCCTGGATA 421 AGAGGTTCCA GAGTCTCCTT GGCCCCACTG TTCATTAGAT TCTTGTCACG TAGCCATTCT 481 TCCAGTTGAC TGACATTGTA CCTGATCTGC ATGCCCTTGC TCCAGGAGCA CATGTCCTTG 541 CGCAGGAGGA GGTTGTTTAG GGTGATGGCG CCCACAATGT AGAACATCTG TTTGACTACC 601 TGCTTGATTA GCTCCGGGTC CATGCCATGC TGACACATGA CTGAGTGGAA GGAGTTGAGC 661 TGCCGAAGGA TGGAGTCCAG TGTGTAGGTG CCCTCATCTG CGATACTGGA GGTTCGTTTT 721 CTGAGCCCTG TGGGCTTCAC CCCAGATACG CCCTGAATTG TTTCATGCTC TAGCATACCT 781 GAGACTATCA TTGGCTGAAG AATGTTCTCT AACACCCTCA CAAGCTGCTG ATAGATCTGA 841 ATGGCCAAGT CACTCAGTAC TTGCCGATAT TCAGCAAGGT CAAAATTGGT GAGGCAGTGC 901 TCATTCTGGC GAGACGTGTT GTGCTTCATA AAGCCCTCCC TCTCCACTAT ATTGCTTCAA 961 ACAGTGCAAA AAATCGACAT GTGTTAGAGA GCCCAGAAGA GACAGTTTCA AAATCGGTCA 1021 CTCTTTTTCT TCAGACTTTT TTGATGCTGG TATATGGATG TTCAGCAATG ATCGTTACGT 1081 TTCGTGGATC CAATTCGGTT CAGATTAGGT TCCAAGGCCC A //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Fukuda, M., Slac2-c (synaptotagmin-like protein homologue lacking C2 domains-c), a novel linker protein that interacts with Rab27, myosin Va/VIIa, and actin. J. Biol. Chem. 277 (45): 43096-43103 (2002). PMID 12221080. [link to RRC of NBRP]
reference	Fukuda, M., Slac2-a/melanophilin, the missing link between Rab27 and myosin Va: implications of a tripartite protein complex for melanosome transport. J. Biol. Chem., 277, 12432-12436 (2002). PMID 11856727. [link to RRC of NBRP]

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