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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEF-FLAG-mouse myosin Va BR-tail (Brain type)

Expression vector of mouse brain-type myosin Va tail region (+ exon B, - exon D and F).

Catalog number RDB15284
Resource name pEF-FLAG-mouse myosin Va BR-tail (Brain type)
Clone info. Expression vector of mouse brain (BR)-type myosin Va tail region (+ exon B, - exon D and F), tagged with N-terminal FLAG, EF1 promoter. ｃDNA fragment 3161nt to 5989nt of NM_010864.2 is cloned.
Comment Expression was confirmed by the depositor with immunoblotting. Known differences (at least) : G to A substitution at 3481nt of NM_010864.2 (Gln, Synonym).
Vector backbone pEF-BOS FLAG (plasmid)
Size of vector backbone 5.7 kb
Selectable markers Amp^r
Gene/insert name mouse Myo5a cDNA
Depositor|Developer Fukuda, Mitsunori |
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External Database
mouse Myo5a
          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Fukuda, M. and Kuroda T. S., J. Biol. Chem., 277 (45): 43096-43103, 2002).
          Additional terms and conditions for distribution Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Fukuda, M. and Kuroda T. S., J. Biol. Chem., 277 (45): 43096-43103, 2002)。
          付加的提供条件 pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB15284 pEF-FLAG-mouse myosin Va BR-tail (Brain type) DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Fukuda, M., Slac2-c (synaptotagmin-like protein homologue lacking C2 domains-c), a novel linker protein that interacts with Rab27, myosin Va/VIIa, and actin. J. Biol. Chem. 277 (45): 43096-43103 (2002). PMID 12221080.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15284_A7Gop1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EF1a-C_F
          Sequence file: RDB15284_A7Goa.seq check
          >D04773A2_A7Go_2_EF1a-C_F_B07_04_ABI24.ab1
              1 TAAGGCCATT GCTTTTTGAG TTTGGATCTT GGTTCATTCT CAAGCCTCAG ACAGTGGTTC
             61 AAAGTTTTTT TCTTCCATTT CAGGTGTCGT GAGGAATTCT CTAGAGCGGC CGCGGGAATT
            121 CGATTCCACC ATGGACTACA AGGACGATGA CGACAAGGGA TCCGCTCGCT CTGTGGAACG
            181 CTACAAGAAG CTCCATATTG GCATGGAGAA CAAGATTATG CAGCTGCAGC GCAAAGTGGA
            241 TGAGCAGAAT AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTGG AAGGAGTATA
            301 CAACTCTGAG ACTGAAAAAC TACGAAATGA TGTAGAACGT CTTCAGCTAA GTGAAGAGGA
            361 AGCTAAGGTT GCCACTGGGC GGGTCCTTAG TCTGCAGGAA GAAATTGCAA AACTCCGAAA
            421 AGACCTGGAA CAAACTCGAT CAGAGAAAAA GTCTATTGAA GAAAGAGCAG ATAAATACAA
            481 ACAGGAAACT GACCAGCTGG TGTCAAACTT GAAGGAAGAA AATACTTTGC TGAAGCAGGA
            541 AAAGGAGACC CTCAACCACC GCATTGTGGA GCAGGCGAAG GAGATGACAG AAACTATGGA
            601 GAGGAAGTTA GTAGAAGAGA CAAAACAACT GGAGCTTGAC CTGAATGATG AGAGGCTGAG
            661 GTATCAGAAC CTTCTGAATG AGTTCAGTCG CCTGGAGGAG CGCTATGATG ACCTCAAGGA
            721 AGAGATGACC CTGATGCTGA ATGTGCCTAA GCCAGGACAC AAGAGAACAG ACTCTACCCA
            781 CAGCAGTAAT GAGTCTGAAT ACACCTTCAG CTCTGAGTTT GCAGAAACTG AAGACATTGC
            841 ACCAAGAACA GAGGAGCCAA TTGAGAAGAA GGTGCCTCTG GATATGTCAC TATTCCTTAA
            901 GCTCCAGAAG CGTGTCACAG AGCTGGGAAC AGGAGAAGCA GCTGATGCAG GATGAGCTGG
            961 GACCGCAGGA GGAAGCAGTT GTTTCCGCAG CAGGCAAAGA GAGAATGGGC ACAGATAGAG
           1021 AGCTGACTAG AGTATGAGTC TCTCAGCGTC AGACTTGCAG TCCGTAACCG AAAAACTGAA
          1081 AAACCGAGCC TG
          //
          Primer: hCGSF_R
          Sequence file: RDB15284_A7Gob.seq check
          >D04773A2_A7Go_2_hGCSF_R_B08_05_ABI24.ab1
              1 CTGGACTGAT CTCTCAGGGT CCAGGTGGGG ACCCTCACTC TAGAGCGGCC GCTATGGTCG
             61 ACGGTATCGA TAAGCTTGAT ATCGAATTCG ATTCAGACCC GTGCGATGAA GCCCAGGCCC
            121 AGGCTGGCTG GGATCTGGAT GGTTTCTAGG GCTAGGGAGG ATGGGTTAAA GGGAAAAGTG
            181 ACAGGAAAGA TGTGTTTTGC ATCCATGAGC AGCTGTGGAG AGTCTTTCCT GTCTCGTAAC
            241 CGCATCTGTA TAGTGCGGAT AAATGAAACT GAGACCCTTT CTTCAAACTC ATTAACCGGT
            301 GTGTACAGAT TCAACACTTT GACAATCTGG GCCGTGGTCA GGGCGTTGCA CATGGAACAG
            361 ATGGCTTCTG CGTCATCGTC AGTTTTCTTT TTCACTTGCA AAAGCTGAGC GGCCTGGATA
            421 AGAGGTTCCA GAGTCTCCTT GGCCCCACTG TTCATTAGAT TCTTGTCACG TAGCCATTCT
            481 TCCAGTTGAC TGACATTGTA CCTGATCTGC ATGCCCTTGC TCCAGGAGCA CATGTCCTTG
            541 CGCAGGAGGA GGTTGTTTAG GGTGATGGCG CCCACAATGT AGAACATCTG TTTGACTACC
            601 TGCTTGATTA GCTCCGGGTC CATGCCATGC TGACACATGA CTGAGTGGAA GGAGTTGAGC
            661 TGCCGAAGGA TGGAGTCCAG TGTGTAGGTG CCCTCATCTG CGATACTGGA GGTTCGTTTT
            721 CTGAGCCCTG TGGGCTTCAC CCCAGATACG CCCTGAATTG TTTCATGCTC TAGCATACCT
            781 GAGACTATCA TTGGCTGAAG AATGTTCTCT AACACCCTCA CAAGCTGCTG ATAGATCTGA
            841 ATGGCCAAGT CACTCAGTAC TTGCCGATAT TCAGCAAGGT CAAAATTGGT GAGGCAGTGC
            901 TCATTCTGGC GAGACGTGTT GTGCTTCATA AAGCCCTCCC TCTCCACTAT ATTGCTTCAA
            961 ACAGTGCAAA AAATCGACAT GTGTTAGAGA GCCCAGAAGA GACAGTTTCA AAATCGGTCA
           1021 CTCTTTTTCT TCAGACTTTT TTGATGCTGG TATATGGATG TTCAGCAATG ATCGTTACGT
          1081 TTCGTGGATC CAATTCGGTT CAGATTAGGT TCCAAGGCCC A
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Fukuda, M., Slac2-c (synaptotagmin-like protein homologue lacking C2 domains-c), a novel linker protein that interacts with Rab27, myosin Va/VIIa, and actin. J. Biol. Chem. 277 (45): 43096-43103 (2002). PMID 12221080.
          reference Fukuda, M., Slac2-a/melanophilin, the missing link between Rab27 and myosin Va: implications of a tripartite protein complex for melanosome transport. J. Biol. Chem., 277, 12432-12436 (2002). PMID 11856727.

          2022.05.17

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