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pEF-FLAG-mouse myosin Va BR-tail (Brain type) (#RDB15284)

Expression vector of mouse brain-type myosin Va tail region (+ exon B, - exon D and F).

Clone info. Expression vector of mouse brain (BR)-type myosin Va tail region (+ exon B, - exon D and F), tagged with N-terminal FLAG, EF1 promoter. ｃDNA fragment 3161nt to 5989nt of NM_010864.2 is cloned.
Comment Expression was confirmed by the depositor with immunoblotting. Known differences (at least) : G to A substitution at 3481nt of NM_010864.2 (Gln, Synonym).
Vector backbone pEF-BOS FLAG (plasmid)
Size of vector backbone 5.7 kb
Selectable markers Amp^r
Gene/insert name mouse Myo5a cDNA
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Fukuda, M. and Kuroda T. S., J. Biol. Chem., 277 (45): 43096-43103, 2002).
Additional terms and conditions:
Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Fukuda, M. and Kuroda T. S., J. Biol. Chem., 277 (45): 43096-43103, 2002)。
MTAに書く付加的使用条件：
pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15284 pEF-FLAG-mouse myosin Va BR-tail (Brain type) DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEF-FLAG-mouse myosin Va BR-tail (Brain type) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15284).

Reference section:

Fukuda, M., Kuroda, T.S., Slac2-c (synaptotagmin-like protein homologue lacking C2 domains-c), a novel linker protein that interacts with Rab27, myosin Va/VIIa, and actin. J. Biol. Chem. 277 (45): 43096-43103 (2002). PMID 12221080. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15284_A7Gop1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F
Sequence file: RDB15284_A7Goa.seq check
>D04773A2_A7Go_2_EF1a-C_F_B07_04_ABI24.ab1
    1 TAAGGCCATT GCTTTTTGAG TTTGGATCTT GGTTCATTCT CAAGCCTCAG ACAGTGGTTC
   61 AAAGTTTTTT TCTTCCATTT CAGGTGTCGT GAGGAATTCT CTAGAGCGGC CGCGGGAATT
  121 CGATTCCACC ATGGACTACA AGGACGATGA CGACAAGGGA TCCGCTCGCT CTGTGGAACG
  181 CTACAAGAAG CTCCATATTG GCATGGAGAA CAAGATTATG CAGCTGCAGC GCAAAGTGGA
  241 TGAGCAGAAT AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTGG AAGGAGTATA
  301 CAACTCTGAG ACTGAAAAAC TACGAAATGA TGTAGAACGT CTTCAGCTAA GTGAAGAGGA
  361 AGCTAAGGTT GCCACTGGGC GGGTCCTTAG TCTGCAGGAA GAAATTGCAA AACTCCGAAA
  421 AGACCTGGAA CAAACTCGAT CAGAGAAAAA GTCTATTGAA GAAAGAGCAG ATAAATACAA
  481 ACAGGAAACT GACCAGCTGG TGTCAAACTT GAAGGAAGAA AATACTTTGC TGAAGCAGGA
  541 AAAGGAGACC CTCAACCACC GCATTGTGGA GCAGGCGAAG GAGATGACAG AAACTATGGA
  601 GAGGAAGTTA GTAGAAGAGA CAAAACAACT GGAGCTTGAC CTGAATGATG AGAGGCTGAG
  661 GTATCAGAAC CTTCTGAATG AGTTCAGTCG CCTGGAGGAG CGCTATGATG ACCTCAAGGA
  721 AGAGATGACC CTGATGCTGA ATGTGCCTAA GCCAGGACAC AAGAGAACAG ACTCTACCCA
  781 CAGCAGTAAT GAGTCTGAAT ACACCTTCAG CTCTGAGTTT GCAGAAACTG AAGACATTGC
  841 ACCAAGAACA GAGGAGCCAA TTGAGAAGAA GGTGCCTCTG GATATGTCAC TATTCCTTAA
  901 GCTCCAGAAG CGTGTCACAG AGCTGGGAAC AGGAGAAGCA GCTGATGCAG GATGAGCTGG
  961 GACCGCAGGA GGAAGCAGTT GTTTCCGCAG CAGGCAAAGA GAGAATGGGC ACAGATAGAG
 1021 AGCTGACTAG AGTATGAGTC TCTCAGCGTC AGACTTGCAG TCCGTAACCG AAAAACTGAA
1081 AAACCGAGCC TG
//
Primer: hCGSF_R
Sequence file: RDB15284_A7Gob.seq check
>D04773A2_A7Go_2_hGCSF_R_B08_05_ABI24.ab1
    1 CTGGACTGAT CTCTCAGGGT CCAGGTGGGG ACCCTCACTC TAGAGCGGCC GCTATGGTCG
   61 ACGGTATCGA TAAGCTTGAT ATCGAATTCG ATTCAGACCC GTGCGATGAA GCCCAGGCCC
  121 AGGCTGGCTG GGATCTGGAT GGTTTCTAGG GCTAGGGAGG ATGGGTTAAA GGGAAAAGTG
  181 ACAGGAAAGA TGTGTTTTGC ATCCATGAGC AGCTGTGGAG AGTCTTTCCT GTCTCGTAAC
  241 CGCATCTGTA TAGTGCGGAT AAATGAAACT GAGACCCTTT CTTCAAACTC ATTAACCGGT
  301 GTGTACAGAT TCAACACTTT GACAATCTGG GCCGTGGTCA GGGCGTTGCA CATGGAACAG
  361 ATGGCTTCTG CGTCATCGTC AGTTTTCTTT TTCACTTGCA AAAGCTGAGC GGCCTGGATA
  421 AGAGGTTCCA GAGTCTCCTT GGCCCCACTG TTCATTAGAT TCTTGTCACG TAGCCATTCT
  481 TCCAGTTGAC TGACATTGTA CCTGATCTGC ATGCCCTTGC TCCAGGAGCA CATGTCCTTG
  541 CGCAGGAGGA GGTTGTTTAG GGTGATGGCG CCCACAATGT AGAACATCTG TTTGACTACC
  601 TGCTTGATTA GCTCCGGGTC CATGCCATGC TGACACATGA CTGAGTGGAA GGAGTTGAGC
  661 TGCCGAAGGA TGGAGTCCAG TGTGTAGGTG CCCTCATCTG CGATACTGGA GGTTCGTTTT
  721 CTGAGCCCTG TGGGCTTCAC CCCAGATACG CCCTGAATTG TTTCATGCTC TAGCATACCT
  781 GAGACTATCA TTGGCTGAAG AATGTTCTCT AACACCCTCA CAAGCTGCTG ATAGATCTGA
  841 ATGGCCAAGT CACTCAGTAC TTGCCGATAT TCAGCAAGGT CAAAATTGGT GAGGCAGTGC
  901 TCATTCTGGC GAGACGTGTT GTGCTTCATA AAGCCCTCCC TCTCCACTAT ATTGCTTCAA
  961 ACAGTGCAAA AAATCGACAT GTGTTAGAGA GCCCAGAAGA GACAGTTTCA AAATCGGTCA
 1021 CTCTTTTTCT TCAGACTTTT TTGATGCTGG TATATGGATG TTCAGCAATG ATCGTTACGT
1081 TTCGTGGATC CAATTCGGTT CAGATTAGGT TCCAAGGCCC A
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Fukuda, M., Slac2-c (synaptotagmin-like protein homologue lacking C2 domains-c), a novel linker protein that interacts with Rab27, myosin Va/VIIa, and actin. J. Biol. Chem. 277 (45): 43096-43103 (2002). PMID 12221080. [link to RRC of NBRP]
reference Fukuda, M., Slac2-a/melanophilin, the missing link between Rab27 and myosin Va: implications of a tripartite protein complex for melanosome transport. J. Biol. Chem., 277, 12432-12436 (2002). PMID 11856727. [link to RRC of NBRP]