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pEF-FLAG-mouse myosin Va BR-tail (Brain type)

Expression vector of mouse brain-type myosin Va tail region (+ exon B, - exon D and F).

Catalog number RDB15284
Resource name pEF-FLAG-mouse myosin Va BR-tail (Brain type)
Clone info. Expression vector of mouse brain (BR)-type myosin Va tail region (+ exon B, - exon D and F), tagged with N-terminal FLAG, EF1 promoter. cDNA fragment 3161nt to 5989nt of NM_010864.2 is cloned.
Expression was confirmed by the depositor with immunoblotting. Known differences (at least) : G to A substitution at 3481nt of NM_010864.2 (Gln, Synonym).
Vector backbone pEF-BOS FLAG (plasmid)
Size of vector backbone 5.7 kb
Selectable markers Amp^r
Gene/insert name mouse Myo5a cDNA
Depositor Fukuda, Mitsunori |
 
Other clones in our bank mouse Myo5a (NCBI Gene 17918) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Fukuda, M. and Kuroda T. S., J. Biol. Chem., 277 (45): 43096-43103, 2002).
Additional terms and conditions for distribution Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15284 pEF-FLAG-mouse myosin Va BR-tail (Brain type) DNA solution

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References and tips

Electronic file

Featured content

References

original Fukuda, M., Slac2-c (synaptotagmin-like protein homologue lacking C2 domains-c), a novel linker protein that interacts with Rab27, myosin Va/VIIa, and actin. J. Biol. Chem. 277 (45): 43096-43103 (2002). PMID 12221080.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15284_A7Gop1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F RDB15284_A7Goa.seq
>D04773A2_A7Go_2_EF1a-C_F_B07_04_ABI24.ab1
    1 TAAGGCCATT GCTTTTTGAG TTTGGATCTT GGTTCATTCT CAAGCCTCAG ACAGTGGTTC
   61 AAAGTTTTTT TCTTCCATTT CAGGTGTCGT GAGGAATTCT CTAGAGCGGC CGCGGGAATT
  121 CGATTCCACC ATGGACTACA AGGACGATGA CGACAAGGGA TCCGCTCGCT CTGTGGAACG
  181 CTACAAGAAG CTCCATATTG GCATGGAGAA CAAGATTATG CAGCTGCAGC GCAAAGTGGA
  241 TGAGCAGAAT AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTGG AAGGAGTATA
  301 CAACTCTGAG ACTGAAAAAC TACGAAATGA TGTAGAACGT CTTCAGCTAA GTGAAGAGGA
  361 AGCTAAGGTT GCCACTGGGC GGGTCCTTAG TCTGCAGGAA GAAATTGCAA AACTCCGAAA
  421 AGACCTGGAA CAAACTCGAT CAGAGAAAAA GTCTATTGAA GAAAGAGCAG ATAAATACAA
  481 ACAGGAAACT GACCAGCTGG TGTCAAACTT GAAGGAAGAA AATACTTTGC TGAAGCAGGA
  541 AAAGGAGACC CTCAACCACC GCATTGTGGA GCAGGCGAAG GAGATGACAG AAACTATGGA
  601 GAGGAAGTTA GTAGAAGAGA CAAAACAACT GGAGCTTGAC CTGAATGATG AGAGGCTGAG
  661 GTATCAGAAC CTTCTGAATG AGTTCAGTCG CCTGGAGGAG CGCTATGATG ACCTCAAGGA
  721 AGAGATGACC CTGATGCTGA ATGTGCCTAA GCCAGGACAC AAGAGAACAG ACTCTACCCA
  781 CAGCAGTAAT GAGTCTGAAT ACACCTTCAG CTCTGAGTTT GCAGAAACTG AAGACATTGC
  841 ACCAAGAACA GAGGAGCCAA TTGAGAAGAA GGTGCCTCTG GATATGTCAC TATTCCTTAA
  901 GCTCCAGAAG CGTGTCACAG AGCTGGGAAC AGGAGAAGCA GCTGATGCAG GATGAGCTGG
  961 GACCGCAGGA GGAAGCAGTT GTTTCCGCAG CAGGCAAAGA GAGAATGGGC ACAGATAGAG
 1021 AGCTGACTAG AGTATGAGTC TCTCAGCGTC AGACTTGCAG TCCGTAACCG AAAAACTGAA
1081 AAACCGAGCC TG
//
Primer: hCGSF_R RDB15284_A7Gob.seq
>D04773A2_A7Go_2_hGCSF_R_B08_05_ABI24.ab1
    1 CTGGACTGAT CTCTCAGGGT CCAGGTGGGG ACCCTCACTC TAGAGCGGCC GCTATGGTCG
   61 ACGGTATCGA TAAGCTTGAT ATCGAATTCG ATTCAGACCC GTGCGATGAA GCCCAGGCCC
  121 AGGCTGGCTG GGATCTGGAT GGTTTCTAGG GCTAGGGAGG ATGGGTTAAA GGGAAAAGTG
  181 ACAGGAAAGA TGTGTTTTGC ATCCATGAGC AGCTGTGGAG AGTCTTTCCT GTCTCGTAAC
  241 CGCATCTGTA TAGTGCGGAT AAATGAAACT GAGACCCTTT CTTCAAACTC ATTAACCGGT
  301 GTGTACAGAT TCAACACTTT GACAATCTGG GCCGTGGTCA GGGCGTTGCA CATGGAACAG
  361 ATGGCTTCTG CGTCATCGTC AGTTTTCTTT TTCACTTGCA AAAGCTGAGC GGCCTGGATA
  421 AGAGGTTCCA GAGTCTCCTT GGCCCCACTG TTCATTAGAT TCTTGTCACG TAGCCATTCT
  481 TCCAGTTGAC TGACATTGTA CCTGATCTGC ATGCCCTTGC TCCAGGAGCA CATGTCCTTG
  541 CGCAGGAGGA GGTTGTTTAG GGTGATGGCG CCCACAATGT AGAACATCTG TTTGACTACC
  601 TGCTTGATTA GCTCCGGGTC CATGCCATGC TGACACATGA CTGAGTGGAA GGAGTTGAGC
  661 TGCCGAAGGA TGGAGTCCAG TGTGTAGGTG CCCTCATCTG CGATACTGGA GGTTCGTTTT
  721 CTGAGCCCTG TGGGCTTCAC CCCAGATACG CCCTGAATTG TTTCATGCTC TAGCATACCT
  781 GAGACTATCA TTGGCTGAAG AATGTTCTCT AACACCCTCA CAAGCTGCTG ATAGATCTGA
  841 ATGGCCAAGT CACTCAGTAC TTGCCGATAT TCAGCAAGGT CAAAATTGGT GAGGCAGTGC
  901 TCATTCTGGC GAGACGTGTT GTGCTTCATA AAGCCCTCCC TCTCCACTAT ATTGCTTCAA
  961 ACAGTGCAAA AAATCGACAT GTGTTAGAGA GCCCAGAAGA GACAGTTTCA AAATCGGTCA
 1021 CTCTTTTTCT TCAGACTTTT TTGATGCTGG TATATGGATG TTCAGCAATG ATCGTTACGT
1081 TTCGTGGATC CAATTCGGTT CAGATTAGGT TCCAAGGCCC A
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Fukuda, M., Slac2-c (synaptotagmin-like protein homologue lacking C2 domains-c), a novel linker protein that interacts with Rab27, myosin Va/VIIa, and actin. J. Biol. Chem. 277 (45): 43096-43103 (2002). PMID 12221080.
reference Fukuda, M., Slac2-a/melanophilin, the missing link between Rab27 and myosin Va: implications of a tripartite protein complex for melanosome transport. J. Biol. Chem., 277, 12432-12436 (2002). PMID 11856727.

2018.12.10

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