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pEF-FLAG-mouse myosin Va (Brain type)

Expression vector of mouse brain-type myosin Va (Myo5a).

Catalog number RDB15283
Resource name pEF-FLAG-mouse myosin Va (Brain type)
Clone info. Expression vector of mouse brain (BR)-type myosin Va [Myo5a in NCBI], tagged with N-terminal FLAG, EF1 promoter.
Expression was confirmed by the depositor with immunoblotting.
Vector backbone pEF-BOS FLAG (plasmid)
Size of vector backbone 5.7 kb
Selectable markers Amp^r
Gene/insert name mouse Myo5a cDNA
Depositor Fukuda, Mitsunori |
 
Other clones in our bank mouse Myo5a (NCBI Gene 17918) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Fukuda, M. et al., J. Biol. Chem. 277 (14): 12432-12436, 2002).
Additional terms and conditions for distribution Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15283 pEF-FLAG-mouse myosin Va (Brain type) DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Fukuda, M., Slac2-a/melanophilin, the missing link between Rab27 and myosin Va: implications of a tripartite protein complex for melanosome transport. J. Biol. Chem., 277, 12432-12436 (2002). PMID 11856727.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15283_A7Gop1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F RDB15283_A7Goa.seq
>D04773A1_A7Go_1_EF1a-C_F_A07_01_ABI24.ab1
    1 CCAGCATTGC CTTTTTGAGT TTGGATCTTG GTTCATTCTC AAGCCTCAGA CAGTGGTTCA
   61 AAGTTTTTTT CTTCCATTTC AGGTGTCGTG AGGAATTCTC TAGAGCGGCC GCGGGAATTC
  121 GATTCCACCA TGGACTACAA GGACGATGAC GACAAGGGAT CTATGGCCGC GTCCGAGCTC
  181 TACACCAAGT TTGCCAGGGT TTGGATCCCT GATCCTGAGG AAGTGTGGAA ATCGGCAGAG
  241 TTGCTCAAGG ATTATAAGCC TGGAGATAAA GTGCTCCTGC TTCACCTCGA GGAAGGGAAG
  301 GATTTGGAAT ACCGTCTAGA CCCAAAGACC GGTGAGCTCC CTCACTTACG GAACCCTGAC
  361 ATACTTGTTG GAGAAAATGA CCTCACAGCC CTCAGCTACC TTCACGAGCC CGCTGTGCTA
  421 CATAATCTCC GAGTTCGCTT CATCGATTCC AAACTTATTT ATACGTATTG TGGAATAGTT
  481 CTGGTAGCTA TAAATCCCTA TGAGCAGCTG CCTATTTATG GAGAAGATAT TATTAATGCC
  541 TACAGTGGCC AGAACATGGG TGACATGGAT CCTCACATCT TCGCAGTAGC TGAAGAGGCT
  601 TACAAGCAAA TGGCAAGGGA TGAACGAAAT CAGTCCATCA TTGTAAGTGG AGAGTCAGGC
  661 GCAGGGAAGA CGGTCTCTGC TAAGTATGCC ATGCGGTACT TCGCAACTGT AAGTGGCTCT
  721 GCCAGTGAGG CCAATGTTGA GGAAAAGGTC TTGGCCTCCA ACCCCATCAT GGAGTCAATT
  781 GGAAATGCCA AAACAACCAG GAATGATAAT AGCAGCCGGT TTGGAAAATA TATTGAAATT
  841 GGTTTTGACA AGAGGTACAG AATCATCGGT GCCAATATGA GAACTTACCT TTTTAGAGAA
  901 ATCCAGAGTG GTGTTCCAGG CAGAAGAGGG AGAGAAACTA CCATATCTTC TATCAGCTCT
  961 GTGCTTCGGC AAAGTTACCT GAGTTTAAAA TGCTGCGGTT TAGGGAAATG CAGATAGGTT
 1021 TTTCATTTAC ACGAAAGCAA AGGGAGCAGG CCCTAATGAA TTAGAAGAAG TTAGATGATG
1081 CGTAGGAGAT TGGCGCCACA CCAAGGCAAA GGCCTTGC
//
Primer: hCGSF_R RDB15283_A7Gob.seq
>D04773A1_A7Go_1_hGCSF_R_A08_02_ABI24.ab1
    1 CTGGGCTGAA TCTCTCAGGG TCCCAGGTGG GGACCCTCAC TCTAGAGCGG CCGCTATGGT
   61 CGACGGTATC GATAAGCTTG ATATCGAATT CGATTCAGAC CCGTGCGATG AAGCCCAGGC
  121 CCAGGCTGGC TGGGATCTGG ATGGTTTCTA GGGCTAGGGA GGATGGGTTA AAGGGAAAAG
  181 TGACAGGAAA GATGTGTTTT GCATCCATGA GCAGCTGTGG AGAGTCTTTC CTGTCTCGTA
  241 ACCGCATCTG TATAGTGCGG ATAAATGAAA CTGAGACCCT TTCTTCAAAC TCATTAACCG
  301 GTGTGTACAG ATTCAACACT TTGACAATCT GGGCCGTGGT CAGGGCGTTG CACATGGAAC
  361 AGATGGCTTC TGCGTCATCG TCAGTTTTCT TTTTCACTTG CAAAAGCTGA GCGGCCTGGA
  421 TAAGAGGTTC CAGAGTCTCC TTGGCCCCAC TGTTCATTAG ATTCTTGTCA CGTAGCCATT
  481 CTTCCAGTTG ACTGACATTG TACCTGATCT GCATGCCCTT GCTCCAGGAG CACATGTCCT
  541 TGCGCAGGAG GAGGTTGTTT AGGGTGATGG CGCCCACAAT GTAGAACATC TGTTTGACTA
  601 CCTGCTTGAT TAGCTCCGGG TCCATGCCAT GCTGACACAT GACTGAGTGG AAGGAGTTGA
  661 GCTGCCGAAG GATGGAGTCC AGTGTGTAGG TGCCCTCATC TGCGATACTG GAGGTTCGTT
  721 TTCTGAGCCC TGTGGGCTTC ACCCCAGATA CGCCCTGAAT TGTTTCATGC TCTAGCATAC
  781 CTGAGACTAT CATTGGCTGA AGAATGTTCT CTAACACCCT CACAAGCTGC TGATAGATCT
  841 GAATGGCCAA GTCACTCAGT ACTTGCCGAT ATTCAGCAAG GTCAAAATTG GTGAGGCAGT
  901 GCTCATTCTG GCGAGACGTG TTGTGCTTCA TAAAGCCCTC CTCTCCACTA TATTGCTTCA
  961 ACAGTGCAAA AAATCGACAT GTGTTAGAGA GCCAGATGGA GACAGTTTCA AAATCGTCAC
 1021 CCTCTTTTCT TCAGACTTTT TTTTGATGGC TGGTAATGGT GATGTCAGCC AATGATCCTA
1081 CTTCTGAATC AATCGTTCCA GAATTAGGTT CCAGG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Fukuda, M., Slac2-a/melanophilin, the missing link between Rab27 and myosin Va: implications of a tripartite protein complex for melanosome transport. J. Biol. Chem., 277, 12432-12436 (2002). PMID 11856727.

2018.12.10

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