RDB15283 - pEF-FLAG-mouse myosin Va (Brain type)

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pEF-FLAG-mouse myosin Va (Brain type) (#RDB15283)

Expression vector of mouse brain-type myosin Va (Myo5a).

Clone info.	Expression vector of mouse brain (BR)-type myosin Va [Myo5a in NCBI], tagged with N-terminal FLAG, EF1 promoter.
Comment	Expression was confirmed by the depositor with immunoblotting.
Vector backbone	pEF-BOS FLAG (plasmid)
Size of vector backbone	5.7 kb
Selectable markers	Amp^r
Gene/insert name	mouse Myo5a cDNA
Depositor\|Developer	Fukuda, Mitsunori \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Fukuda, M. et al., J. Biol. Chem. 277 (14): 12432-12436, 2002).
Additional terms and conditions:
Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Fukuda, M. et al., J. Biol. Chem. 277 (14): 12432-12436, 2002). Additional terms and conditions: Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Fukuda, M. et al., J. Biol. Chem. 277 (14): 12432-12436, 2002)。 MTAに書く付加的使用条件： pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。

必要書類

提供依頼書

[依頼書の記入例

]
提供同意書 (MTA, 非営利学術目的用)[Word

] [MTAの記入例

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。

MTAに書く使用条件

利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Fukuda, M. et al., J. Biol. Chem. 277 (14): 12432-12436, 2002)。
MTAに書く付加的使用条件：
pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15283	pEF-FLAG-mouse myosin Va (Brain type)	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEF-FLAG-mouse myosin Va (Brain type) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15283).

Reference section:

Fukuda, M., Kuroda, T.S., Mikoshiba, K., Slac2-a/melanophilin, the missing link between Rab27 and myosin Va: implications of a tripartite protein complex for melanosome transport. J. Biol. Chem., 277, 12432-12436 (2002). PMID 11856727. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB15283_A7Gop1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168) Region: EF-1 alpha pro,insert 5'(FLAG,mMyosin Va(Brain type 5')) Sequence file: RDB15283_A7Goc.seq
>D04773A1_A7Go_1_EF1a-C_F_A07_01_ABI24.ab1 1 NNNNNNNNNN NNTTTTGANT TTGGNNCTTG GTTCNTTCTC NAGCCTCAGA CAGTGGTTCA 61 AAGTTTTTTT CTTCCATTTC AGGTGTCGTG AGGAATTCTC TAGAGCGGCC GCGGGAATTC 121 GATTCCACCA TGGACTACAA GGACGATGAC GACAAGGGAT CTATGGCCGC GTCCGAGCTC 181 TACACCAAGT TTGCCAGGGT TTGGATCCCT GATCCTGAGG AAGTGTGGAA ATCGGCAGAG 241 TTGCTCAAGG ATTATAAGCC TGGAGATAAA GTGCTCCTGC TTCACCTCGA GGAAGGGAAG 301 GATTTGGAAT ACCGTCTAGA CCCAAAGACC GGTGAGCTCC CTCACTTACG GAACCCTGAC 361 ATACTTGTTG GAGAAAATGA CCTCACAGCC CTCAGCTACC TTCACGAGCC CGCTGTGCTA 421 CATAATCTCC GAGTTCGCTT CATCGATTCC AAACTTATTT ATACGTATTG TGGAATAGTT 481 CTGGTAGCTA TAAATCCCTA TGAGCAGCTG CCTATTTATG GAGAAGATAT TATTAATGCC 541 TACAGTGGCC AGAACATGGG TGACATGGAT CCTCACATCT TCGCAGTAGC TGAAGAGGCT 601 TACAAGCAAA TGGCAAGGGA TGAACGAAAT CAGTCCATCA TTGTAAGTGG AGAGTCAGGC 661 GCAGGGAAGA CGGTCTCTGC TAAGTATGCC ATGCGGTACT TCGCAACTGT AAGTGGCTCT 721 GCCAGTGAGG CCAATGTTGA NGANNAGGTC TTGGCCTCCA ACCCCATCAT GGAGTCAATT 781 GGAAATGCCA AAACAACCAG GAATGATAAT AGCAGCCGGT TTGGAAAATA TATTGAAATT 841 GGTTTTGACA AGANGTACAG ANTCATCGGN GCCNATATGA GAANTTACCN TTTTAGANAA 901 ATCCAGANNG GNNNTCCAGG NAGANNNNGN ANANAAACTA NNNNANCTTC NATCANCNNN 961 NNNNNNN //
Primer: hGCSF_R (Pr0031) Region: hG-CSF polyA,insert 3'(mMyosin Va(Brain type 3')) Sequence file: RDB15283_A7God.seq
>D04773A1_A7Go_1_hGCSF_R_A08_02_ABI24.ab1 1 NNNNNNNNNN NNNNNNAGGG TCNNNGGNGG GGNNCCTCAC TCTAGAGCGG CCGCTATGGT 61 CGACGGTATC GATAAGCTTG ATATCGAATT CGATTCAGAC CCGTGCGATG AAGCCCAGGC 121 CCAGGCTGGC TGGGATCTGG ATGGTTTCTA GGGCTAGGGA GGATGGGTTA AAGGGAAAAG 181 TGACAGGAAA GATGTGTTTT GCATCCATGA GCAGCTGTGG AGAGTCTTTC CTGTCTCGTA 241 ACCGCATCTG TATAGTGCGG ATAAATGAAA CTGAGACCCT TTCTTCAAAC TCATTAACCG 301 GTGTGTACAG ATTCAACACT TTGACAATCT GGGCCGTGGT CAGGGCGTTG CACATGGAAC 361 AGATGGCTTC TGCGTCATCG TCAGTTTTCT TTTTCACTTG CAAAAGCTGA GCGGCCTGGA 421 TAAGAGGTTC CAGAGTCTCC TTGGCCCCAC TGTTCATTAG ATTCTTGTCA CGTAGCCATT 481 CTTCCAGTTG ACTGACATTG TACCTGATCT GCATGCCCTT GCTCCAGGAG CACATGTCCT 541 TGCGCAGGAG GAGGTTGTTT AGGGTGATGG CGCCCACAAT GTAGAACATC TGTTTGACTA 601 CCTGCTTGAT TAGCTCCGGG TCCATGCCAT GCTGACACAT GACTGAGTGG AAGGAGTTGA 661 GCTGCCGAAN GATGGAGTCC AGTGTGTAGG TGCCCTCATC TGCGATACTG GANGTTCGTT 721 TTCTGAGCCC TGTGGGCTTC ACCCCAGATA CGCCCTGAAT TGTTTCATGC TCTAGCATAC 781 CTGANACTAT CATTGGCTGA AGAATGNTCT CTAACACCCT CACAAGCTGC TGATAGATCT 841 GAATGGNCNA GTCACTCAGT ACTNGCNGAT ATTCAGCAAG GNCAAAATTG GTGANGNAGN 901 GCTCATTCTG GNGANACGNG TNNNGCTTCN TAAAGCCCNN CNNNNCNCTA TATNGNTTNN 961 NNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Fukuda, M., Slac2-a/melanophilin, the missing link between Rab27 and myosin Va: implications of a tripartite protein complex for melanosome transport. J. Biol. Chem., 277, 12432-12436 (2002). PMID 11856727. [link to RRC of NBRP]

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mouse Myo5a

External Database
mouse Myo5a

Reference sequence