Sequencing and PCR primers for primer information.
Expression vector of mouse brain-type myosin Va (Myo5a).
| Catalog number | RDB15283 |
|---|---|
| Resource name | pEF-FLAG-mouse myosin Va (Brain type) |
| Clone info. | Expression vector of mouse brain (BR)-type myosin Va [Myo5a in NCBI], tagged with N-terminal FLAG, EF1 promoter. Expression was confirmed by the depositor with immunoblotting. |
| Vector backbone | pEF-BOS FLAG (plasmid) |
| Size of vector backbone | 5.7 kb |
| Selectable markers | Amp^r |
| Gene/insert name | mouse Myo5a cDNA |
| Depositor | Fukuda, Mitsunori | |
| Other clones in our bank | mouse Myo5a (NCBI Gene 17918) | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Fukuda, M. et al., J. Biol. Chem. 277 (14): 12432-12436, 2002). |
|---|---|
| Additional terms and conditions for distribution | Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested. |
| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB15283 | pEF-FLAG-mouse myosin Va (Brain type) | DNA solution |
Electronic file
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References
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RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB15283_A7Gop1.pdf |
|---|
Nucleotide sequence of a portion of this resource (if available).
| Primer: EF1a-C_F | RDB15283_A7Goa.seq |
|---|---|
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>D04773A1_A7Go_1_EF1a-C_F_A07_01_ABI24.ab1 1 CCAGCATTGC CTTTTTGAGT TTGGATCTTG GTTCATTCTC AAGCCTCAGA CAGTGGTTCA 61 AAGTTTTTTT CTTCCATTTC AGGTGTCGTG AGGAATTCTC TAGAGCGGCC GCGGGAATTC 121 GATTCCACCA TGGACTACAA GGACGATGAC GACAAGGGAT CTATGGCCGC GTCCGAGCTC 181 TACACCAAGT TTGCCAGGGT TTGGATCCCT GATCCTGAGG AAGTGTGGAA ATCGGCAGAG 241 TTGCTCAAGG ATTATAAGCC TGGAGATAAA GTGCTCCTGC TTCACCTCGA GGAAGGGAAG 301 GATTTGGAAT ACCGTCTAGA CCCAAAGACC GGTGAGCTCC CTCACTTACG GAACCCTGAC 361 ATACTTGTTG GAGAAAATGA CCTCACAGCC CTCAGCTACC TTCACGAGCC CGCTGTGCTA 421 CATAATCTCC GAGTTCGCTT CATCGATTCC AAACTTATTT ATACGTATTG TGGAATAGTT 481 CTGGTAGCTA TAAATCCCTA TGAGCAGCTG CCTATTTATG GAGAAGATAT TATTAATGCC 541 TACAGTGGCC AGAACATGGG TGACATGGAT CCTCACATCT TCGCAGTAGC TGAAGAGGCT 601 TACAAGCAAA TGGCAAGGGA TGAACGAAAT CAGTCCATCA TTGTAAGTGG AGAGTCAGGC 661 GCAGGGAAGA CGGTCTCTGC TAAGTATGCC ATGCGGTACT TCGCAACTGT AAGTGGCTCT 721 GCCAGTGAGG CCAATGTTGA GGAAAAGGTC TTGGCCTCCA ACCCCATCAT GGAGTCAATT 781 GGAAATGCCA AAACAACCAG GAATGATAAT AGCAGCCGGT TTGGAAAATA TATTGAAATT 841 GGTTTTGACA AGAGGTACAG AATCATCGGT GCCAATATGA GAACTTACCT TTTTAGAGAA 901 ATCCAGAGTG GTGTTCCAGG CAGAAGAGGG AGAGAAACTA CCATATCTTC TATCAGCTCT 961 GTGCTTCGGC AAAGTTACCT GAGTTTAAAA TGCTGCGGTT TAGGGAAATG CAGATAGGTT 1021 TTTCATTTAC ACGAAAGCAA AGGGAGCAGG CCCTAATGAA TTAGAAGAAG TTAGATGATG 1081 CGTAGGAGAT TGGCGCCACA CCAAGGCAAA GGCCTTGC // |
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| Primer: hCGSF_R | RDB15283_A7Gob.seq |
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>D04773A1_A7Go_1_hGCSF_R_A08_02_ABI24.ab1 1 CTGGGCTGAA TCTCTCAGGG TCCCAGGTGG GGACCCTCAC TCTAGAGCGG CCGCTATGGT 61 CGACGGTATC GATAAGCTTG ATATCGAATT CGATTCAGAC CCGTGCGATG AAGCCCAGGC 121 CCAGGCTGGC TGGGATCTGG ATGGTTTCTA GGGCTAGGGA GGATGGGTTA AAGGGAAAAG 181 TGACAGGAAA GATGTGTTTT GCATCCATGA GCAGCTGTGG AGAGTCTTTC CTGTCTCGTA 241 ACCGCATCTG TATAGTGCGG ATAAATGAAA CTGAGACCCT TTCTTCAAAC TCATTAACCG 301 GTGTGTACAG ATTCAACACT TTGACAATCT GGGCCGTGGT CAGGGCGTTG CACATGGAAC 361 AGATGGCTTC TGCGTCATCG TCAGTTTTCT TTTTCACTTG CAAAAGCTGA GCGGCCTGGA 421 TAAGAGGTTC CAGAGTCTCC TTGGCCCCAC TGTTCATTAG ATTCTTGTCA CGTAGCCATT 481 CTTCCAGTTG ACTGACATTG TACCTGATCT GCATGCCCTT GCTCCAGGAG CACATGTCCT 541 TGCGCAGGAG GAGGTTGTTT AGGGTGATGG CGCCCACAAT GTAGAACATC TGTTTGACTA 601 CCTGCTTGAT TAGCTCCGGG TCCATGCCAT GCTGACACAT GACTGAGTGG AAGGAGTTGA 661 GCTGCCGAAG GATGGAGTCC AGTGTGTAGG TGCCCTCATC TGCGATACTG GAGGTTCGTT 721 TTCTGAGCCC TGTGGGCTTC ACCCCAGATA CGCCCTGAAT TGTTTCATGC TCTAGCATAC 781 CTGAGACTAT CATTGGCTGA AGAATGTTCT CTAACACCCT CACAAGCTGC TGATAGATCT 841 GAATGGCCAA GTCACTCAGT ACTTGCCGAT ATTCAGCAAG GTCAAAATTG GTGAGGCAGT 901 GCTCATTCTG GCGAGACGTG TTGTGCTTCA TAAAGCCCTC CTCTCCACTA TATTGCTTCA 961 ACAGTGCAAA AAATCGACAT GTGTTAGAGA GCCAGATGGA GACAGTTTCA AAATCGTCAC 1021 CCTCTTTTCT TCAGACTTTT TTTTGATGGC TGGTAATGGT GATGTCAGCC AATGATCCTA 1081 CTTCTGAATC AATCGTTCCA GAATTAGGTT CCAGG // |
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Please visit Sequencing and PCR primers for primer information.
References
2018.12.10
GNP_filter3_RDBDEP_html_181012.pl