Resource data sheet
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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEF-FLAG-mouse VAMP-7 (#RDB15173)

Expression vector of mouse vesicle-associated membrane protein 7 (VAMP-7).

Clone info. Expression vector of mouse vesicle-associated membrane protein 7 (VAMP-7), tagged with N-terminal FLAG, EF1 promoter.
Comment Expression was confirmed by the depositor with immunoprecipitation and immunoblotting.
Vector backbone pEF-BOS FLAG (Plasmid)
Size of vector backbone 5.7 kb
Selectable markers Amp^r
Gene/insert name mouse Vamp7 cDNA
Depositor|Developer Fukuda, Mitsunori |
 
Sequence (partial) RDB15173pka01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (J. Biol. Chem. 286 (9): 7507-7521, 2011)
Additional terms and conditions:
Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(J. Biol. Chem. 286 (9): 7507-7521, 2011)。
MTAに書く付加的使用条件：
pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15173 pEF-FLAG-mouse VAMP-7 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEF-FLAG-mouse VAMP-7 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15173).

Reference section:

Tamura, K., Ohbayashi, N., Ishibashi, K., Fukuda, M., Structure-function analysis of VPS9-ankyrin-repeat protein (Varp) in the trafficking of tyrosinase-related protein 1 in melanocytes. J. Biol. Chem. 286 (9): 7507-7521 (2011). PMID 21187289. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15173_A7Fmp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C ()
Sequence file: RDB15173_A7Fma.seq check
>D04709A8_2_A7Fm_EF1a-C_F_E02_14_ABI24.ab1
    1 CAGGGGATGG CCTTTTTGAG TTTGGATCTT GGTTCATTCT CAAGCCTCAG ACAGTGGTTC
   61 AAAGTTTTTT TCTTCCATTT CAGGTGTCGT GAGGAATTCT CTAGAGCGGC CGCGGGAATT
  121 CGATTCCACC ATGGACTACA AGGACGATGA CGACAAGGGA TCCATGGCCA TTCTTTTTGC
  181 TGTTGTTGCC AGGGGAACCA CTATCCTTGC CAAACATGCT TGGTGTGGAG GAAACTTCCT
  241 GGAGGTGACA GAGCAGATTC TGGCTAAGAT ACCTTCTGAA AATAATAAAC TAACTTACTC
  301 ACATGGCAAT TATTTGTTTC ATTACATCTG CCAAGACAGG ATTGTGTATC TTTGTATCAC
  361 TGATGATGAT TTTGAGCGTT CTCGAGCGTT CAGTTTTTTG AATGAAGTAA AGAAGAGATT
  421 CCAGACAACT TACGGTTCAA GAGCACAAAC TGCACTTCCT TATGCTATGA ATAGCGAGTT
  481 TTCAAGTGTT TTGGCTGCAC AACTGAAGCA TCACTCTGAG AATAAGAGCC TAGACAAAGT
  541 GATGGAGACT CAAGCACAAG TGGATGAACT GAAAGGAATA ATGGTCAGAA ACATAGATTT
  601 AGTTGCTCAA CGTGGAGAAA GGTTGGAATT GCTGATAGAT AAAACAGAAA ACCTTGTGGA
  661 TTCATCTGTC ACTTTCAAAA CTACCAGCAG AAATCTTGCT CGAGCCATGT GTATGAAGAA
  721 TATCAAGCTC ACTATCATCA TCATCATTGT ATCAATTGTG TTCATCTATA TCATTGTGTC
  781 ACTTCTCTGT GGTGGGTTTA CATGGCCAAA CTGTGTGAAG AAATAAAATC ACTAGTGAAT
  841 TCGCGGCCGC TCTAGAGTGA GGGTCCCCAC CTGGGACCCT TGAGAGTATC AGGTCTCCCA
  901 CGTGGGAGAC AAGAAATCCC TGTTTAATAT TTAAACAGCA GTGTTCCCCA TCTGGGTCCT
  961 TGCACCCCTC ACTCTGGCCC TCAGCCGACT GCACAGCGGG CCCCTGCATC CCCTTTGGCT
 1021 GTGAGGCCCC TGGACAAGCA GAGGTGGCCA GAGCTGGGGA GCATGACCCT GGGGTCCCAC
 1081 GATTTGCTGG GGATCTCGTT TTCTCTAGAC TTTTGGAACT GGATTGACTC CGGACCATTC
1141 AACGACGTGT CTCCTGAATT TTCTTGGGGG TGGCCCTTC
//
Primer: hGCSF-R ()
Sequence file: RDB15173_A7Fmb.seq check
>D04709A8_2_A7Fm_hGCSF-R_F02_17_ABI24.ab1
    1 GTGGGGACTG ATACTCTCAA GGGTCCCAGG TGGGGACCCT CACTCTAGAG CGGCCGCGAA
   61 TTCACTAGTG ATTTTATTTC TTCACACAGT TTGGCCATGT AAACCCACCA CAGAGAAGTG
  121 ACACAATGAT ATAGATGAAC ACAATTGATA CAATGATGAT GATGATAGTG AGCTTGATAT
  181 TCTTCATACA CATGGCTCGA GCAAGATTTC TGCTGGTAGT TTTGAAAGTG ACAGATGAAT
  241 CCACAAGGTT TTCTGTTTTA TCTATCAGCA ATTCCAACCT TTCTCCACGT TGAGCAACTA
  301 AATCTATGTT TCTGACCATT ATTCCTTTCA GTTCATCCAC TTGTGCTTGA GTCTCCATCA
  361 CTTTGTCTAG GCTCTTATTC TCAGAGTGAT GCTTCAGTTG TGCAGCCAAA ACACTTGAAA
  421 ACTCGCTATT CATAGCATAA GGAAGTGCAG TTTGTGCTCT TGAACCGTAA GTTGTCTGGA
  481 ATCTCTTCTT TACTTCATTC AAAAAACTGA ACGCTCGAGA ACGCTCAAAA TCATCATCAG
  541 TGATACAAAG ATACACAATC CTGTCTTGGC AGATGTAATG AAACAAATAA TTGCCATGTG
  601 AGTAAGTTAG TTTATTATTT TCAGAAGGTA TCTTAGCCAG AATCTGCTCT GTCACCTCCA
  661 GGAAGTTTCC TCCACACCAA GCATGTTTGG CAAGGATAGT GGTTCCCCTG GCAACAACAG
  721 CAAAAAGAAT GGCCATGGAT CCCTTGTCGT CATCGTCCTT GTAGTCCATG GTGGAATCGA
  781 ATTCCCGCGG CCGCTCTAGA GAATTCCTCA CGACACCTGA AATGGAAGAA AAAAACTTTG
  841 AACCACTGTC TGAGGCTTGA GAATGAACCA AGATCCAAAC TCAAAAAGGG CAAATTCCAA
  901 GGAGAATTAC ATCAAGTGCC AAGCTGGCCT AACTTCAGTC TCCACCCACT CAGTGTGGGG
  961 AAACTCCATC GCATAAAACC CCTCCCCCCA ACCTAAAGAC GACGTACTCC AAAAGCTCGA
 1021 GAACTAATCG AGGTGCCTGG ACGGCGCCCG GGTACTCCGT GGAGTCACAT GAAGCGACGC
 1081 TGAGACGAAA GGCCTTTTCC TTTGTGTGGG TGACTCACCC GCCCGCTCTC CGAGCGCCGC
1141 GTCTTCATTT GAGCTCCTGC CAGCAGGACG GAAAGCCGGC CATCTTTTTC CGGCCTTC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Tamura, K., Structure-function analysis of VPS9-ankyrin-repeat protein (Varp) in the trafficking of tyrosinase-related protein 1 in melanocytes. J. Biol. Chem. 286 (9): 7507-7521 (2011). PMID 21187289. [link to RRC of NBRP]