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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEF-FLAG-mouse VAMP-7

Expression vector of mouse vesicle-associated membrane protein 7 (VAMP-7).

Catalog number RDB15173
Resource name pEF-FLAG-mouse VAMP-7
Clone info. Expression vector of mouse vesicle-associated membrane protein 7 (VAMP-7), tagged with N-terminal FLAG, EF1 promoter.
Comment Expression was confirmed by the depositor with immunoprecipitation and immunoblotting.
Vector backbone pEF-BOS FLAG (Plasmid)
Size of vector backbone 5.7 kb
Selectable markers Amp^r
Gene/insert name mouse Vamp7 cDNA
Depositor|Developer Fukuda, Mitsunori |
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External Database
mouse Vamp7

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (J. Biol. Chem. 286 (9): 7507-7521, 2011)
          Additional terms and conditions for distribution Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(J. Biol. Chem. 286 (9): 7507-7521, 2011)。
          付加的提供条件 pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB15173 pEF-FLAG-mouse VAMP-7 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence (partial) RDB15173pka01.seq provided by the depositor

          Original reference

          original Tamura, K., Structure-function analysis of VPS9-ankyrin-repeat protein (Varp) in the trafficking of tyrosinase-related protein 1 in melanocytes. J. Biol. Chem. 286 (9): 7507-7521 (2011). PMID 21187289.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15173_A7Fmp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EF1a-C
          Sequence file: RDB15173_A7Fma.seq check
          >D04709A8_2_A7Fm_EF1a-C_F_E02_14_ABI24.ab1
              1 CAGGGGATGG CCTTTTTGAG TTTGGATCTT GGTTCATTCT CAAGCCTCAG ACAGTGGTTC
             61 AAAGTTTTTT TCTTCCATTT CAGGTGTCGT GAGGAATTCT CTAGAGCGGC CGCGGGAATT
            121 CGATTCCACC ATGGACTACA AGGACGATGA CGACAAGGGA TCCATGGCCA TTCTTTTTGC
            181 TGTTGTTGCC AGGGGAACCA CTATCCTTGC CAAACATGCT TGGTGTGGAG GAAACTTCCT
            241 GGAGGTGACA GAGCAGATTC TGGCTAAGAT ACCTTCTGAA AATAATAAAC TAACTTACTC
            301 ACATGGCAAT TATTTGTTTC ATTACATCTG CCAAGACAGG ATTGTGTATC TTTGTATCAC
            361 TGATGATGAT TTTGAGCGTT CTCGAGCGTT CAGTTTTTTG AATGAAGTAA AGAAGAGATT
            421 CCAGACAACT TACGGTTCAA GAGCACAAAC TGCACTTCCT TATGCTATGA ATAGCGAGTT
            481 TTCAAGTGTT TTGGCTGCAC AACTGAAGCA TCACTCTGAG AATAAGAGCC TAGACAAAGT
            541 GATGGAGACT CAAGCACAAG TGGATGAACT GAAAGGAATA ATGGTCAGAA ACATAGATTT
            601 AGTTGCTCAA CGTGGAGAAA GGTTGGAATT GCTGATAGAT AAAACAGAAA ACCTTGTGGA
            661 TTCATCTGTC ACTTTCAAAA CTACCAGCAG AAATCTTGCT CGAGCCATGT GTATGAAGAA
            721 TATCAAGCTC ACTATCATCA TCATCATTGT ATCAATTGTG TTCATCTATA TCATTGTGTC
            781 ACTTCTCTGT GGTGGGTTTA CATGGCCAAA CTGTGTGAAG AAATAAAATC ACTAGTGAAT
            841 TCGCGGCCGC TCTAGAGTGA GGGTCCCCAC CTGGGACCCT TGAGAGTATC AGGTCTCCCA
            901 CGTGGGAGAC AAGAAATCCC TGTTTAATAT TTAAACAGCA GTGTTCCCCA TCTGGGTCCT
            961 TGCACCCCTC ACTCTGGCCC TCAGCCGACT GCACAGCGGG CCCCTGCATC CCCTTTGGCT
           1021 GTGAGGCCCC TGGACAAGCA GAGGTGGCCA GAGCTGGGGA GCATGACCCT GGGGTCCCAC
           1081 GATTTGCTGG GGATCTCGTT TTCTCTAGAC TTTTGGAACT GGATTGACTC CGGACCATTC
          1141 AACGACGTGT CTCCTGAATT TTCTTGGGGG TGGCCCTTC
          //
          Primer: hGCSF-R
          Sequence file: RDB15173_A7Fmb.seq check
          >D04709A8_2_A7Fm_hGCSF-R_F02_17_ABI24.ab1
              1 GTGGGGACTG ATACTCTCAA GGGTCCCAGG TGGGGACCCT CACTCTAGAG CGGCCGCGAA
             61 TTCACTAGTG ATTTTATTTC TTCACACAGT TTGGCCATGT AAACCCACCA CAGAGAAGTG
            121 ACACAATGAT ATAGATGAAC ACAATTGATA CAATGATGAT GATGATAGTG AGCTTGATAT
            181 TCTTCATACA CATGGCTCGA GCAAGATTTC TGCTGGTAGT TTTGAAAGTG ACAGATGAAT
            241 CCACAAGGTT TTCTGTTTTA TCTATCAGCA ATTCCAACCT TTCTCCACGT TGAGCAACTA
            301 AATCTATGTT TCTGACCATT ATTCCTTTCA GTTCATCCAC TTGTGCTTGA GTCTCCATCA
            361 CTTTGTCTAG GCTCTTATTC TCAGAGTGAT GCTTCAGTTG TGCAGCCAAA ACACTTGAAA
            421 ACTCGCTATT CATAGCATAA GGAAGTGCAG TTTGTGCTCT TGAACCGTAA GTTGTCTGGA
            481 ATCTCTTCTT TACTTCATTC AAAAAACTGA ACGCTCGAGA ACGCTCAAAA TCATCATCAG
            541 TGATACAAAG ATACACAATC CTGTCTTGGC AGATGTAATG AAACAAATAA TTGCCATGTG
            601 AGTAAGTTAG TTTATTATTT TCAGAAGGTA TCTTAGCCAG AATCTGCTCT GTCACCTCCA
            661 GGAAGTTTCC TCCACACCAA GCATGTTTGG CAAGGATAGT GGTTCCCCTG GCAACAACAG
            721 CAAAAAGAAT GGCCATGGAT CCCTTGTCGT CATCGTCCTT GTAGTCCATG GTGGAATCGA
            781 ATTCCCGCGG CCGCTCTAGA GAATTCCTCA CGACACCTGA AATGGAAGAA AAAAACTTTG
            841 AACCACTGTC TGAGGCTTGA GAATGAACCA AGATCCAAAC TCAAAAAGGG CAAATTCCAA
            901 GGAGAATTAC ATCAAGTGCC AAGCTGGCCT AACTTCAGTC TCCACCCACT CAGTGTGGGG
            961 AAACTCCATC GCATAAAACC CCTCCCCCCA ACCTAAAGAC GACGTACTCC AAAAGCTCGA
           1021 GAACTAATCG AGGTGCCTGG ACGGCGCCCG GGTACTCCGT GGAGTCACAT GAAGCGACGC
           1081 TGAGACGAAA GGCCTTTTCC TTTGTGTGGG TGACTCACCC GCCCGCTCTC CGAGCGCCGC
          1141 GTCTTCATTT GAGCTCCTGC CAGCAGGACG GAAAGCCGGC CATCTTTTTC CGGCCTTC
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Tamura, K., Structure-function analysis of VPS9-ankyrin-repeat protein (Varp) in the trafficking of tyrosinase-related protein 1 in melanocytes. J. Biol. Chem. 286 (9): 7507-7521 (2011). PMID 21187289.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl