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CALR-mCherry-KDEL (#RDB15152)

Expression vector of rat Calreticulin (CALR), mCherry-ER retention signal (KDEL) tag.

Clone info. Expression vector of rat Calreticulin (CALR) with C-terminally tagged mCherry-ER retention signal (KDEL).
Comment Expression was confirmed by the depositor with confocal lase scanning microscopy.
Vector backbone pmCherry-N1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name rat Calr cDNA
Depositor|Developer Hamachi, Itaru |
 

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Nat. Methods 13 (11): 931-937, 2016)
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RDB15152 CALR-mCherry-KDEL DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The CALR-mCherry-KDEL was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15152).

Reference section:

Miki, T., Awa, M., Nishikawa, Y., Kiyonaka, S., Wakabayashi, M., Ishihama, Y., Hamachi, I., A conditional proteomics approach to identify proteins involved in zinc homeostasis. Nat. Methods 13 (11): 931-937 (2016). PMID 27617391. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15152_A7Flp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB15152_A7Fla.seq check
>D04425A1_A7Fl_2_CMV-Forward_A07_01_ABI24.ab1
    1 CGTTAACCTC CGCCCCATTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
   61 TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCCGCTAGCG CTACCGGACT CAGATCTCGA
  121 GGCCACCATG CTCCTTTCGG TGCCGCTCCT GCTTGGCCTC CTCGGCCTGG CTGCCGCAGA
  181 CCCTGCCATC TATTTCAAAG AGCAGTTCTT GGACGGAGAT GCCTGGACCA ACCGCTGGGT
  241 CGAATCCAAA CATAAGTCTG ATTTTGGCAA ATTCGTCCTC AGTTCTGGCA AATTCTACGG
  301 GGACCAGGAG AAGGATAAAG GGTTGCAGAC AAGCCAAGAT GCCCGATTTT ACGCGCTGTC
  361 CGCCAGATTC GAACCCTTCA GCAACAAGGG CCAGACACTG GTGGTACAGT TCACCGTGAA
  421 GCATGAGCAG AATATCGACT GTGGGGGCGG CTACGTGAAG CTGTTTCCGG GTGGCTTGGA
  481 CCAGAAGGAC ATGCATGGAG ACTCAGAATA TAACATCATG TTTGGTCCGG ACATCTGCGG
  541 TCCTGGCACC AAGAAGGTTC ATGTCATCTT TAACTACAAG GGCAAGAACG TGCTGATCAA
  601 CAAGGATATC CGGTGTAAGG ATGATGAATT CACACATCTA TACACGCTGA TTGTGCGGCC
  661 AGACAACACC TACGAGGTGA AAATTGACAA CAGCCAGGTG GAGTCGGGCT CCTTGGAGGA
  721 TGATTGGGAC TTTCTGCCGC CCAAGAAGAT TAAGGATCCT GACGCTGCCA AGCCAGAAGA
  781 CTGGGATGAA CGAGCCAAGA TTGATGACCC CACAGATTCC AAGCCTGAGG ACTGGGACAA
  841 GCCAGAGCAC ATCCCTGACC CTGATGCTAA GAAGCCTGAG GACTGGGACG AAGAGATGGA
  901 TGGAGAGTGG GAACCACCAG TGATTCAAAA TCCTGAAATA CAAGGGCGAA ATGGAAGCCC
  961 ACGTCAAATT TGACACCCAG ATTACAAGGG TACCCTGCAT ACACCCAGAG ATTGAACATC
 1021 CCTGATACTC CCCCGATGCC GATATCCTAT GGCCCTAATG ATAGTTTTGC TGTACCTGAG
1081 TCTTAGACTC TGCCGAGTCA GTCTCGCACT AAATTCATGC
//
Primer: mCherry-N_rev
Sequence file: RDB15152_A7Flb.seq check
>D04425A1_A7Fl_2_mCherry-N_R_B07_04_ABI24.ab1
    1 TACATAACTC TTGATGATGG CCATGTTATC CTCCTCGCCC TTGCTCACCA TGGTGGCGAC
   61 CGGTGGATCC CGGGCCCGCG GCAGCTCATC CTTGGCTTGG CCAGTGGCAT CCTCCTCATC
  121 TTCTTCCTTC TCATCCTCTT CATCTTCATC TTCATCTCTG TCATCCTCAT CCTCTTTATC
  181 CTCGGCCTCC TCTTCCTCTT TACGCTTCTT GTCTTCTTCT TCTTCCTTAA GCCTCTGCTC
  241 CTCATCCTGC TTGTCCTTCA TCTGCTTCTC TGCAGCCTTG GTGACACCCC AGGTCTCATT
  301 GCCAAACTCC TCTGCATAGG CCTCATCATT GGTGATGAGG AAGTTGTCAA AAATTGTGCC
  361 AGACTTGACC TGCCAGAGGT CTAAGCCCAG TACAGCAAAA CTATCATAGG CATAGATATT
  421 CGCATCGGGG GAGTATTCAG GATTGTCAAT CTCTGGGTGT ATCCAGGTAC CCTTGTAATC
  481 TGGGTTGTCA ATTTGACGTG GCTTCCATTC GCCCTTGTAT TCAGGATTTT GAATCACTGG
  541 TGGTTCCCAC TCTCCATCCA TCTCTTCGTC CCAGTCCTCA GGCTTCTTAG CATCAGGGTC
  601 AGGGATGTGC TCTGGCTTGT CCCAGTCCTC AGGCTTGGAA TCTGTGGGGT CATCAATCTT
  661 GGCTCGTTCA TCCCAGTCTT CTGGCTTGGC AGCGTCAGGA TCCTTAATCT TCTTGGGCGG
  721 CAGAAAGTCC CAATCATCCT CCAAGGAGCC CGACTCCACC TGGCTGTTGT CAATTTTCAC
  781 CTCGTAGGTG TTGTCTGGCC GCACAATCAG CGTGTATAGA TGTGTGAATT CATCATCCTT
  841 ACACCGGATA TCCTTGTTGA TCAGCACGTT CTTGCCCTTG TAGTTAAAGA TGACATGAAC
  901 CTTCTTGGTG CCAGGACCGC AGATGTCCGG ACCAAACATG ATGTTATATT CTGAGTCTCC
  961 ATGCATGTCC TTCTGGTCCA AGCCCACCCG GGAAAACAGC TTTCACGTAG CCCGCCCCCC
 1021 ACAGGTCGAT ATTCTGCTCA TGCCTCACGG TGACTGGTAC ACCAGCGTCT ATCGTGCTGC
1081 TGAGGCTTCG ATTCTTGGAT CGGA
//
Primer: SV40polyA-R
Sequence file: RDB15152_A7Flc.seq check
>D04425A1_A7Fl_2_SV40polyA-R_C07_07_ABI24.ab1
    1 AAAAATGGGA GCTTTTTAAG CAAGTAAAAC CTCTACAAAT GTGGTATGGC TGATTATGAT
   61 CTAGAGTCGC GGCCGCTAGA GCTCATCCTT CTTGTACAGC TCGTCCATGC CGCCGGTGGA
  121 GTGGCGGCCC TCGGCGCGTT CGTACTGTTC CACGATGGTG TAGTCCTCGT TGTGGGAGGT
  181 GATGTCCAAC TTGATGTTGA CGTTGTAGGC GCCGGGCAGC TGCACGGGCT TCTTGGCCTT
  241 GTAGGTGGTC TTGACCTCAG CGTCGTAGTG GCCGCCGTCC TTCAGCTTCA GCCTCTGCTT
  301 GATCTCGCCC TTCAGGGCGC CGTCCTCGGG GTACATCCGC TCGGAGGAGG CCTCCCAGCC
  361 CATGGTCTTC TTCTGCATTA CGGGGCCGTC GGAGGGGAAG TTGGTGCCGC GCAGCTTCAC
  421 CTTGTAGATG AACTCGCCGT CCTGCAGGGA GGAGTCCTGG GTCACGGTCA CCACGCCGCC
  481 GTCCTCGAAG TTCATCACGC GCTCCCACTT GAAGCCCTCG GGGAAGGACA GCTTCAAGTA
  541 GTCGGGGATG TCGGCGGGGT GCTTCACGTA GGCCTTGGAG CCGTACATGA ACTGAGGGGA
  601 CAGGATGTCC CAGGCGAAGG GCAGGGGGCC ACCCTTGGTC ACCTTCAGCT TGGCGGTCTG
  661 GGTGCCCTCG TAGGGGCGGC CCTCGCCCTC GCCCTCGATC TCGAACTCGT GGCCGTTCAC
  721 GGAGCCCTCC ATGTGCACCT TGAAGCGCAT GAACTCCTTG ATGATGGCCA TGTTATCCTC
  781 CTCGCCCTTG CTCACCATGG TGGCGACCGG TGGATCCCGG GCCCGCGGCA GCTCATCCTT
  841 GGCTTGGCCA GTGGCATCCT CCTCATCTTC TTCCTTCTCA TCCTCTTCAT CTTCATCTTC
  901 ATCTCTGTCA TCCTCATCCT CTTTATCCTC GGCCTCCTCT TCCTCTTTAC GCTTCTTGTC
  961 TTCTTCTTTC TTCCCTTAAA GCCTCTGCTC CTCATCCTGC TTTGTCCCTT TCATCTGCTT
 1021 CTCTGCCAGC CTTGGATGAC ACCCCCAGGT CTCATTTGGC CCAAACTCCG TCTGCATAGG
1081 CGTCAATCAT TGTGAATGGA AGGAAGTTGT TCAAAAAAAT CG
//
Primer: SV40pro_F_V2
Sequence file: RDB15152_A7Fld.seq check
>D04425A1_2_A7Fl_SV40pro_F_V2_E09_15_ABI24.ab1
    1 GGCCGGCATT TTGGAGCTAG GCTTTTGCAA GATCGATCAA GAGACAGGAT GAGGATCGTT
   61 TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT
  121 ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT
  181 GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA
  241 ACTGCAAGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC
  301 TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG
  361 GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC
  421 AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA
  481 TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA
  541 CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGAGCATGCC
  601 CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA
  661 AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA
  721 GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG
  781 CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT
  841 TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGGTTCGAA ATGACCGACC AAGCGACGCC
  901 CAACCCTGCC ATCACGAGAT TTCGATTCCA CCGCCCGCCC TTCTATGAAG GTTGGGGCTT
  961 CGGAATCGTT TTCCCGGGGA CGCCCGGCTG GATGATCCCT CCCAGCGCGG GGATCTCATG
 1021 CTGGGAGTTT CCTTTCGCCA ACCCTAGGGG GAGGCTTACC TGGAAACACG GATGGAGACA
1081 ATTACCTGAG GAACCGCCGC TTATGAACCG TCCAATAAAA GGAACAGGCA T
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Miki, T., A conditional proteomics approach to identify proteins involved in zinc homeostasis. Nat. Methods 13 (11): 931-937 (2016). PMID 27617391. [link to RRC of NBRP]