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pEGFP-C1-human EVI5

Expression vector of human ecotropic viral integration site 5 (EVI5), fused with N-terminal EGFP, CMV promoter.

Catalog number RDB15021
Resource name pEGFP-C1-human EVI5
Clone info. Expression vector of human ecotropic viral integration site 5 (EVI5), fused with N-terminal EGFP, CMV promoter. This clone is an unregistered variant of human EVI5 on NCBI and contains DNA fragment corresponding 11nt to 2476nt with 13 nucleotides (CAGTCCACATCAG) insertion between 2161nt and 2162nt, which caused frame-shift of protein coding region.
Expression was confirmed by the depositor with fluorescence microscope. Known differences compared with NM_001308248.1 (at least) : synonymous substitutions, C to T at 118nt, C to T at 583nt and G to A at 1867nt.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name human EVI5 cDNA
Depositor Fukuda, Mitsunori |
 
Other clones in our bank human EVI5 (NCBI Gene 7813) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Itoh, T. and Fukuda, M., J. Biol. Chem., 281(42): 31823-31831, 2006)
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Schedule A (GFP-A) [Link]
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RDB15021 pEGFP-C1-human EVI5 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Itoh, T., Identification of EPI64 as a GTPase-activating protein specific for Rab27A. J. Biol. Chem. 281 (42): 31823-31831 (2006). PMID 16923811.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15021_A7Egp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2
Sequence file: RDB15021_A7Ega.seq check
>D04556F6_A7Eg_2_EGFP-C2_C04_07_ABI24.ab1
    1 TTAACCAACG AGAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC GCCGGGATCA
   61 CTCTCGGCAT GGACGAGCTG TACAAGTCCG GACTCAGATC CATGGTTACC AACAAAATGA
  121 CTGCTGCCTT TAGAAACCCT AGTGGGAAAC AGGTGGCGAC AGACAAAGTT GCAGAAAAGC
  181 TGAGCTCTAC TCTCTCATGG GTGAAGAATA CAGTATCGCA TACAGTCAGT CAGATGGCCA
  241 GTCAGGTGGC AAGTCCATCT ACTTCATTAC ATACCACATC CTCATCTACC ACACTATCAA
  301 CACCAGCCCT TTCACCATCT TCCCCATCAC AGTTGAGTCC AGACGACTTA GAACTCCTGG
  361 CTAAACTGGA AGAACAGAAT AGATTGTTAG AAACGGATAG TAAGTCTTTA AGATCTGTAA
  421 ATGGGTCAAG AAGAAACAGT GGCTCTTCTC TTGTGTCGAG TTCATCAGCC TCTAGCAACC
  481 TCAGTCACCT TGAAGAAGAT TCTTGGATTC TTTGGGGAAG AATTGTTAAT GAATGGGAAG
  541 ATGTACGCAA AAAGAAGGAA AAGCAAGTTA AGGAACTTGT TCATAAAGGG ATACCCCATC
  601 ACTTTAGAGC AATAGTTTGG CAACTTTTAT GCAGTGCACA AAGTATGCCA ATTAAGGATC
  661 AGTATTCAGA ACTTCTGAAA ATGACCTCGC CTTGTGAAAA ATTGATCCGA AGGGACATTG
  721 CTAGAACTTA CCCTGAACAC AACTTTTTTA AGGAAAAAGA TAGCCTTGGA CAGGAGGTTT
  781 TATTTAATGT AATGAAGGCT TACTCTTTAG TAGATCGTGA GGTTGGTTAC TGTCGAGGAA
  841 GTGCTTTTAT AGTTGGATTG TTGCTTATGC AGATGCCAGA AGAAGAAGCT TTCTGTGTAT
  901 TTGTTAAATT AATGCAAGAT TATAGACTTC GTGAACTTTT TAAACCAAGT ATGGCAGAAT
  961 TTGGGCCTTT GTATGTACCA GTTTGAATGT ATGATACAGG AGCATCTTTC CAGAGCTCTT
 1021 TGTACATTTT CAATCTCAGA GTTTTCATAC CTCAATGTAT GCATCATCCT GCTTTCTGAC
 1081 TATCTTTCTT ACACTTTTCA ACTACACTGC ACAGGATATT TTGAATATCC TTTATGTCTG
1141 AGGATTAGAA TTAGTGTTTC GGTGTAGGAT TAGGCCAACT TTCTTTCGGA GTGTGAAA
//
Primer: SV40polyA-R
Sequence file: RDB15021_A7Egb.seq check
>D04556F6_A7Eg_2_SV40polyA_R_E09_15_ABI24.ab1
    1 AAAGTGGGAG TTTTTAGCAG TAAAACCTCT ACAAATGTGG TATGGCTGAT TATGATCAGT
   61 TATCTAGATC CGGTGGATCC CGGGCCCGCG GTACCGTCGA CCTGCAGGCG GCCGCGAATT
  121 CACTAGTGAT TTCAGACAGT GGTTGAATAC GACTCTCTTC TTCTCGGGGG CCGCTCCTTT
  181 TGAACCACTT GGTTGCTCTC TCTGGTCTCC AGCACACTGT CTTCTGTTTC GCTCTCACTA
  241 CCATCTGCCA CTGCGGGGTC CAAAGACATC GAACCAGATT TTCCGTGCAA AGGAAAACCA
  301 ACACCAGTTT CCTGTAAGGA ATTATCTATA AAATCTTCAT CGGAGGAATG GAATGATTCA
  361 TCATCTCCTA TTAAATGGTT GACAATGTGG ATTCCATCAA AAGGAGGTTG GCCTGAGAAG
  421 CCCCTCTGGC CTTTTAGGCA CCTGAGCTGA TGTGGACTGC TCATGATTCA GCTCTGCTAT
  481 CTGATCTTTC AGTTCCCCAA TATACTGGTT AGAATCAGAC TTGTTAAGCT GTCCTTGAAG
  541 CTTTCCTTCT TCTTTCTGGA TTTCAAGCTC AGCAATGTGT TGTCGTAGTT CAGCCACAGC
  601 AGCTATGCTA TCTGCTTCCC GAAGCCTCAC AGCCATCACT TCTTCCTTAT TCTTGCATTC
  661 AATCTCTGCT TGTTTACGCT TTGCTTCACT TAATTGAGTA AGGAGTCCTT TGTTCTGTGC
  721 AGAAAGATAC TGCACTTTCT CCTGTAGGCT AATTACCTCT TGTTCTGCTC TTCGAAGATG
  781 GTTACTATTG ATCTGGTTCT GTGTTTCCAT TTCCATCATC CTTTGTTTTA TTTCTCTTAT
  841 TTCTGCTTGT GTTTCAGCTT CTCTAAGTCG AATGGTCATC AGTTCATCTT GTAACTCATT
  901 CATAGCATTT TTTCTTGGGT GGGTCTTTCC ATCTCCCAGT AGTACGAGCT AAGTGGCGCT
  961 GCCAGTGTTC CTCTAATCCC TTGACTTGCT GTCTAAGTTT CTTTCAAACC CATAATGGGC
 1021 TTCTGCTTTC TCTAAGTTTC ACAGCATGAA GTTCTTCCTG GAGGCATGCA ATATTATTTC
1081 TCATTCAGAA GAAGTATCCT ATCTCTATAT CAGAACTTTT ATTTCC
//
Primer: SV40pro_F_V2
Sequence file: RDB15021_A7Egc.seq check
>D04556F6_A7Eg_2_SV40pro_F_V2_C11_08_ABI24.ab1
    1 GACGAGCTTT TTGGAGCCTA GGCTTTTGCA AGATCGATCA AGAGACAGGA TGAGGATCGT
   61 TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC
  121 TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC
  181 TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG
  241 AACTGCAAGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG
  301 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG
  361 GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG
  421 CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC
  481 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG
  541 ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGAGCATGC
  601 CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG
  661 AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC
  721 AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC
  781 GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC
  841 TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGTTCGAA ATGACCGACC AAGCGACGCC
  901 CAACCCTGCC ATCACGAGAT TTCGATTTCC ACCGCCGCCC TTCTATGAAA GGTTTGGGGC
  961 TTCGGATCGT TTTCCAGGGA CGCCGGCTGG GATGATCTCA GCGCGGGGGA TCTCATGCCT
 1021 GAGGTCTCGC CCAACGTAGA GGAGGCTTAA CCTGAAACAC CGGAGATCAT TACTGAAGGA
1081 CCCGCGCGCC TATTGACCGG ACTA
//
Primer: CMV-Forward
Sequence file: RDB15021_A7Egd.seq check
>D04556F6_A7Eg_2_CMV Forward_G01_07_ABI08.ab1
    1 CGTTCAACTC CGCCCCATTG ACGCAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT
   61 AAGCAGAGCT GGTTTAGTGA ACCGTCAGAT CCGCTAGCGC TACCGGTCGC CACCATGGTG
  121 AGCAAGGGCG AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC
  181 GTAAACGGCC ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC CTACGGCAAG
  241 CTGACCCTGA AGTTCATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG
  301 ACCACCCTGA CCTACGGCGT GCAGTGCTTC AGCCGCTACC CCGACCACAT GAAGCAGCAC
  361 GACTTCTTCA AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT CTTCTTCAAG
  421 GACGACGGCA ACTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC CCTGGTGAAC
  481 CGCATCGAGC TGAAGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG GCACAAGCTG
  541 GAGTACAACT ACAACAGCCA CAACGTCTAT ATCATGGCCG ACAAGCAGAA GAACGGCATC
  601 AAGGTGAACT TCAAGATCCG CCACAACATC GAGGACGGCA GCGTGCAGCT CGCCGACCAC
  661 TACCAGCAGA ACACCCCCAT CGGCGACGGC CCCGTGCTGC TGCCCGACAA CCACTACCTG
  721 AGCACCCAGT CCGCCCTGAG CAAAGACCCC AACGAGAAGC GCGATCACAT GGTCCTGCTG
  781 GAGTTCGTGA CCGCCGCCGG GATCACTCTC GGCATGGACG AGCTGTACAA GTCCGGACTC
  841 AGATCCATGG TTACCAACAA AATGACTGCT GCCTTTAGAA ACCCTAGTGG GAAACAGGTG
  901 GCGACAGACA AAGTTGCAGA AAAGCTGAGC TCTACTCTCT CATGGGTGAA GGAATACAGT
  961 ATCGCATACA GTCAGTCAGA TGGCCCAGTT CAGGTGGGCA AGTCCATCTA CTTTCATTTA
 1021 ACATACCAAC ATCCTCATCT ACCACACTAT CCACCACAGC CTTTTCAACA TGATCGCAAT
1081 CACAGGTGAA GTCCAGAACG AACTTAGAAA GCTTCCCG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Itoh, T., Identification of EPI64 as a GTPase-activating protein specific for Rab27A. J. Biol. Chem. 281 (42): 31823-31831 (2006). PMID 16923811.
reference Itoh, T., Screening for target Rabs of TBC (Tre-2/Bub2/Cdc16) domain-containing proteins based on their Rab-binding activity. Genes Cells 11 (9): 1023-1037 (2006). PMID 16923123.

2019.12.12

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