RDB15021 - pEGFP-C1-human EVI5

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pEGFP-C1-human EVI5

Expression vector of human ecotropic viral integration site 5 (EVI5), fused with N-terminal EGFP, CMV promoter.

Catalog number	RDB15021
Resource name	pEGFP-C1-human EVI5
Clone info.	Expression vector of human ecotropic viral integration site 5 (EVI5), fused with N-terminal EGFP, CMV promoter. This clone is an unregistered variant of human EVI5 on NCBI and contains DNA fragment corresponding 11nt to 2476nt with 13 nucleotides (CAGTCCACATCAG) insertion between 2161nt and 2162nt, which caused frame-shift of protein coding region.
Comment	Expression was confirmed by the depositor with fluorescence microscope. Known differences compared with NM_001308248.1 (at least) : synonymous substitutions, C to T at 118nt, C to T at 583nt and G to A at 1867nt.
Vector backbone	pEGFP-C1 (plasmid)
Size of vector backbone	4.7 kb
Selectable markers	Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name	human EVI5 cDNA
Depositor\|Developer	Fukuda, Mitsunori \|

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human EVI5

External Database
human EVI5

Reference sequence

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Itoh, T. and Fukuda, M., J. Biol. Chem., 281(42): 31823-31831, 2006)

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Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Itoh, T. and Fukuda, M., J. Biol. Chem., 281(42): 31823-31831, 2006)
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Itoh, T. and Fukuda, M., J. Biol. Chem., 281(42): 31823-31831, 2006)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15021	pEGFP-C1-human EVI5	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

References and tips

Original reference

original	Itoh, T., Identification of EPI64 as a GTPase-activating protein specific for Rab27A. J. Biol. Chem. 281 (42): 31823-31831 (2006). PMID 16923811.

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB15021_A7Egp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 Sequence file: RDB15021_A7Ega.seq
>D04556F6_A7Eg_2_EGFP-C2_C04_07_ABI24.ab1 1 TTAACCAACG AGAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC GCCGGGATCA 61 CTCTCGGCAT GGACGAGCTG TACAAGTCCG GACTCAGATC CATGGTTACC AACAAAATGA 121 CTGCTGCCTT TAGAAACCCT AGTGGGAAAC AGGTGGCGAC AGACAAAGTT GCAGAAAAGC 181 TGAGCTCTAC TCTCTCATGG GTGAAGAATA CAGTATCGCA TACAGTCAGT CAGATGGCCA 241 GTCAGGTGGC AAGTCCATCT ACTTCATTAC ATACCACATC CTCATCTACC ACACTATCAA 301 CACCAGCCCT TTCACCATCT TCCCCATCAC AGTTGAGTCC AGACGACTTA GAACTCCTGG 361 CTAAACTGGA AGAACAGAAT AGATTGTTAG AAACGGATAG TAAGTCTTTA AGATCTGTAA 421 ATGGGTCAAG AAGAAACAGT GGCTCTTCTC TTGTGTCGAG TTCATCAGCC TCTAGCAACC 481 TCAGTCACCT TGAAGAAGAT TCTTGGATTC TTTGGGGAAG AATTGTTAAT GAATGGGAAG 541 ATGTACGCAA AAAGAAGGAA AAGCAAGTTA AGGAACTTGT TCATAAAGGG ATACCCCATC 601 ACTTTAGAGC AATAGTTTGG CAACTTTTAT GCAGTGCACA AAGTATGCCA ATTAAGGATC 661 AGTATTCAGA ACTTCTGAAA ATGACCTCGC CTTGTGAAAA ATTGATCCGA AGGGACATTG 721 CTAGAACTTA CCCTGAACAC AACTTTTTTA AGGAAAAAGA TAGCCTTGGA CAGGAGGTTT 781 TATTTAATGT AATGAAGGCT TACTCTTTAG TAGATCGTGA GGTTGGTTAC TGTCGAGGAA 841 GTGCTTTTAT AGTTGGATTG TTGCTTATGC AGATGCCAGA AGAAGAAGCT TTCTGTGTAT 901 TTGTTAAATT AATGCAAGAT TATAGACTTC GTGAACTTTT TAAACCAAGT ATGGCAGAAT 961 TTGGGCCTTT GTATGTACCA GTTTGAATGT ATGATACAGG AGCATCTTTC CAGAGCTCTT 1021 TGTACATTTT CAATCTCAGA GTTTTCATAC CTCAATGTAT GCATCATCCT GCTTTCTGAC 1081 TATCTTTCTT ACACTTTTCA ACTACACTGC ACAGGATATT TTGAATATCC TTTATGTCTG 1141 AGGATTAGAA TTAGTGTTTC GGTGTAGGAT TAGGCCAACT TTCTTTCGGA GTGTGAAA //
Primer: SV40polyA-R Sequence file: RDB15021_A7Egb.seq
>D04556F6_A7Eg_2_SV40polyA_R_E09_15_ABI24.ab1 1 AAAGTGGGAG TTTTTAGCAG TAAAACCTCT ACAAATGTGG TATGGCTGAT TATGATCAGT 61 TATCTAGATC CGGTGGATCC CGGGCCCGCG GTACCGTCGA CCTGCAGGCG GCCGCGAATT 121 CACTAGTGAT TTCAGACAGT GGTTGAATAC GACTCTCTTC TTCTCGGGGG CCGCTCCTTT 181 TGAACCACTT GGTTGCTCTC TCTGGTCTCC AGCACACTGT CTTCTGTTTC GCTCTCACTA 241 CCATCTGCCA CTGCGGGGTC CAAAGACATC GAACCAGATT TTCCGTGCAA AGGAAAACCA 301 ACACCAGTTT CCTGTAAGGA ATTATCTATA AAATCTTCAT CGGAGGAATG GAATGATTCA 361 TCATCTCCTA TTAAATGGTT GACAATGTGG ATTCCATCAA AAGGAGGTTG GCCTGAGAAG 421 CCCCTCTGGC CTTTTAGGCA CCTGAGCTGA TGTGGACTGC TCATGATTCA GCTCTGCTAT 481 CTGATCTTTC AGTTCCCCAA TATACTGGTT AGAATCAGAC TTGTTAAGCT GTCCTTGAAG 541 CTTTCCTTCT TCTTTCTGGA TTTCAAGCTC AGCAATGTGT TGTCGTAGTT CAGCCACAGC 601 AGCTATGCTA TCTGCTTCCC GAAGCCTCAC AGCCATCACT TCTTCCTTAT TCTTGCATTC 661 AATCTCTGCT TGTTTACGCT TTGCTTCACT TAATTGAGTA AGGAGTCCTT TGTTCTGTGC 721 AGAAAGATAC TGCACTTTCT CCTGTAGGCT AATTACCTCT TGTTCTGCTC TTCGAAGATG 781 GTTACTATTG ATCTGGTTCT GTGTTTCCAT TTCCATCATC CTTTGTTTTA TTTCTCTTAT 841 TTCTGCTTGT GTTTCAGCTT CTCTAAGTCG AATGGTCATC AGTTCATCTT GTAACTCATT 901 CATAGCATTT TTTCTTGGGT GGGTCTTTCC ATCTCCCAGT AGTACGAGCT AAGTGGCGCT 961 GCCAGTGTTC CTCTAATCCC TTGACTTGCT GTCTAAGTTT CTTTCAAACC CATAATGGGC 1021 TTCTGCTTTC TCTAAGTTTC ACAGCATGAA GTTCTTCCTG GAGGCATGCA ATATTATTTC 1081 TCATTCAGAA GAAGTATCCT ATCTCTATAT CAGAACTTTT ATTTCC //
Primer: SV40pro_F_V2 Sequence file: RDB15021_A7Egc.seq
>D04556F6_A7Eg_2_SV40pro_F_V2_C11_08_ABI24.ab1 1 GACGAGCTTT TTGGAGCCTA GGCTTTTGCA AGATCGATCA AGAGACAGGA TGAGGATCGT 61 TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC 121 TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC 181 TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG 241 AACTGCAAGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG 301 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG 361 GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG 421 CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC 481 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG 541 ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGAGCATGC 601 CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG 661 AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC 721 AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC 781 GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC 841 TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGTTCGAA ATGACCGACC AAGCGACGCC 901 CAACCCTGCC ATCACGAGAT TTCGATTTCC ACCGCCGCCC TTCTATGAAA GGTTTGGGGC 961 TTCGGATCGT TTTCCAGGGA CGCCGGCTGG GATGATCTCA GCGCGGGGGA TCTCATGCCT 1021 GAGGTCTCGC CCAACGTAGA GGAGGCTTAA CCTGAAACAC CGGAGATCAT TACTGAAGGA 1081 CCCGCGCGCC TATTGACCGG ACTA //
Primer: CMV-Forward Sequence file: RDB15021_A7Egd.seq
>D04556F6_A7Eg_2_CMV Forward_G01_07_ABI08.ab1 1 CGTTCAACTC CGCCCCATTG ACGCAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT 61 AAGCAGAGCT GGTTTAGTGA ACCGTCAGAT CCGCTAGCGC TACCGGTCGC CACCATGGTG 121 AGCAAGGGCG AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC 181 GTAAACGGCC ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC CTACGGCAAG 241 CTGACCCTGA AGTTCATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG 301 ACCACCCTGA CCTACGGCGT GCAGTGCTTC AGCCGCTACC CCGACCACAT GAAGCAGCAC 361 GACTTCTTCA AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT CTTCTTCAAG 421 GACGACGGCA ACTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC CCTGGTGAAC 481 CGCATCGAGC TGAAGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG GCACAAGCTG 541 GAGTACAACT ACAACAGCCA CAACGTCTAT ATCATGGCCG ACAAGCAGAA GAACGGCATC 601 AAGGTGAACT TCAAGATCCG CCACAACATC GAGGACGGCA GCGTGCAGCT CGCCGACCAC 661 TACCAGCAGA ACACCCCCAT CGGCGACGGC CCCGTGCTGC TGCCCGACAA CCACTACCTG 721 AGCACCCAGT CCGCCCTGAG CAAAGACCCC AACGAGAAGC GCGATCACAT GGTCCTGCTG 781 GAGTTCGTGA CCGCCGCCGG GATCACTCTC GGCATGGACG AGCTGTACAA GTCCGGACTC 841 AGATCCATGG TTACCAACAA AATGACTGCT GCCTTTAGAA ACCCTAGTGG GAAACAGGTG 901 GCGACAGACA AAGTTGCAGA AAAGCTGAGC TCTACTCTCT CATGGGTGAA GGAATACAGT 961 ATCGCATACA GTCAGTCAGA TGGCCCAGTT CAGGTGGGCA AGTCCATCTA CTTTCATTTA 1021 ACATACCAAC ATCCTCATCT ACCACACTAT CCACCACAGC CTTTTCAACA TGATCGCAAT 1081 CACAGGTGAA GTCCAGAACG AACTTAGAAA GCTTCCCG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Itoh, T., Identification of EPI64 as a GTPase-activating protein specific for Rab27A. J. Biol. Chem. 281 (42): 31823-31831 (2006). PMID 16923811.
reference	Itoh, T., Screening for target Rabs of TBC (Tre-2/Bub2/Cdc16) domain-containing proteins based on their Rab-binding activity. Genes Cells 11 (9): 1023-1037 (2006). PMID 16923123.

2023.04.10

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