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pEGFP-C1-human TBC1D7 (#RDB14989)

Expression vector of human TBC1 domain family member 7 (TBC1D7), fused with N-terminal EGFP, CMV promoter.

Alternative name FLJ32666
Clone info. Expression vector of human TBC1 domain family member 7 (TBC1D7), fused with N-terminal EGFP, CMV promoter.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name human TBC1D7 cDNA
Reference of insert sequence AB449888 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Ishibashi, K. et al. Genes Cells, 14(1): 41-52, 2009)
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提供依頼書  [依頼書の記入例 ]
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ishibashi, K. et al. Genes Cells, 14(1): 41-52, 2009)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14989 pEGFP-C1-human TBC1D7 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-human TBC1D7 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14989).

Reference section:

Ishibashi, K., Kanno, E., Itoh, T., Fukuda, M., Identification and characterization of a novel Tre-2/Bub2/Cdc16 (TBC) protein that possesses Rab3A-GAP activity. Genes Cells 14 (1): 41-52 (2009). PMID 19077034. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14989_A7Egp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 ()
Sequence file: RDB14989_A7Ega.seq check
>D04556C1_1_EGFP-C2_A01_01_ABI24.ab1
    1 GTCCCCACGA GCAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC GCCGGGATCA
   61 CTCTCGGCAT GGACGAGCTG TACAAGTCCG GACTCAGATC TATGACTGAG GACTCTCAGA
  121 GAAACTTTCG TTCAGTATAT TATGAGAAAG TGGGGTTTCG TGGAGTTGAA GAAAAGAAAT
  181 CATTAGAAAT TCTCCTAAAA GATGACCGTC TGGATACTGA GAAACTTTGT ACTTTTAGTC
  241 AGAGGTTCCC TCTCCCGTCC ATGTACCGTG CATTGGTATG GAAGGTGCTT CTAGGAATCT
  301 TGCCTCCACA CCACGAGTCC CATGCCAAGG TGATGATGTA TCGTAAGGAG CAGTACTTGG
  361 ATGTCCTTCA TGCCCTGAAA GTCGTTCGCT TTGTTAGTGA TGCCACACCT CAGGCTGAAG
  421 TCTATCTCCG CATGTATCAG CTGGAGTCTG GGAAGTTACC TCGAAGTCCC TCTTTTCCAC
  481 TGGAGCCAGA TGATGAAGTG TTTCTTGCCA TAGCTAAAGC CATGGAGGAA ATGGTGGAAG
  541 ATAGTGTCGA CTGTTACTGG ATCACCCGAC GCTTTGTGAA CCAATTAAAT ACCAAGTACC
  601 GGGATTCCTT GCCCCAGTTG CCAAAAGCGT TTGAACAATA CTTGAATCTG GAAGATGGCA
  661 GACTGCTGAC TCATCTGAGG ATGTGTTCCG CGGCGCCCAA ACTTCCTTAT GATCTCTGGT
  721 TCAAGAGGTG CTTTGCGGGA TGTTTGCCTG AATCCAGTTT ACAGAGGGTT TGGGATAAAG
  781 TTGTGAGTGG ATCCTGTAAG ATCCTAGTTT TTGTAGCTGT CGAAATTTTA TTAACCTTTA
  841 AAATAAAAGT TATGGCACTG AACAGTGCAG AGAAGATAAC AAAGTTTCTG GAAAATATTC
  901 CCCAGGACAG CTCAGACGCG ATCGTGAGCA AGGCCATTGA CTTGTGGCAC AAACACTGTG
  961 GGACCCCGGT CCATTCAAGC TGAATCACTA GTGAAATTCG CGGGCCGCCT GCAGTCGACG
 1021 GTACCGCCGG CCGGATCACG GGATCTAGAA TACTGAATCA TAATTCAGTC ATACCACATT
1081 TGGTAGAGTT TACTTGCTAA AAAACCTCCC
//
Primer: pAxEF_R2 ()
Sequence file: RDB14989_A7Egb.seq check
>D04556C1_1_pAxEF_R2_B01_04_ABI24.ab1
    1 AGGGTTAAAA ATGCCTTTAT TTGTGAATTT GTGATGCTAT TGCTTTATTT GTAACCATTA
   61 TAAGCTGCAA TAAACAAGTT AACAACAACA ATTGCATTCA TTTTATGTTT CAGGTTCAGG
  121 GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT AAAACCTCTA CAAATGTGGT ATGGCTGATT
  181 ATGATCAGTT ATCTAGATCC GGTGGATCCC GGGCCCGCGG TACCGTCGAC CTGCAGGCGG
  241 CCGCGAATTC ACTAGTGATT CAGCTTGAAT GGACCGGGGT CCCACAGTGT TTGTGCCACA
  301 AGTCAATGGC CTTGCTCACG ATCGCGTCTG AGCTGTCCTG GGGAATATTT TCCAGAAACT
  361 TTGTTATCTT CTCTGCACTG TTCAGTGCCA TAACTTTTAT TTTAAAGGTT AATAAAATTT
  421 CGACAGCTAC AAAAACTAGG ATCTTACAGG ATCCACTCAC AACTTTATCC CAAACCCTCT
  481 GTAAACTGGA TTCAGGCAAA CATCCCGCAA AGCACCTCTT GAACCAGAGA TCATAAGGAA
  541 GTTTGGGCGC CGCGGAACAC ATCCTCAGAT GAGTCAGCAG TCTGCCATCT TCCAGATTCA
  601 AGTATTGTTC AAACGCTTTT GGCAACTGGG GCAAGGAATC CCGGTACTTG GTATTTAATT
  661 GGTTCACAAA GCGTCGGGTG ATCCAGTAAC AGTCGACACT ATCTTCCACC ATTTCCTCCA
  721 TGGCTTTAGC TATGGCAAGA AACACTTCAT CATCTGGCTC CAGTGGAAAA GAGGGACTTC
  781 GAGGTAACTT CCCAGACTCC AGCTGATACA TGCGGAGATA GACTTCAGCC TGAGGTGTGG
  841 CATCACTAAC AAAGCGAACG ACTTTCAGGG CATGAAGGAC ATCCAAGTAC TGCTCCTTAC
  901 GATACATCAT CACCTTGGCA TGGGGACTCG TGGGTGTGGA GGCAAGATTC CCTAGAAGCA
  961 CCTTTCCATA CCAATGCACG GGTACATGGG ACGGGAGAGG GAACCTCTGA CTAAAAAGTA
 1021 CAAAGTTTTC TCAGTATCAG ACGGTCATCT TTAAGAGAAT TCTTATTGAA TTTCGTTTCT
1081 CAACTTCACG AACGCTCACC TTTCTCTCCA TAAATTATAT AACCTG
//
Primer: CMV_Forward ()
Sequence file: RDB14989_A7Egc.seq check
>D04556C1_1_CMV Forward_C01_07_ABI24.ab1
    1 CGTTTCACTC CGCCCATTGA CGCAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
   61 AGCAGAGCTG GTTTAGTGAA CCGTCAGATC CGCTAGCGCT ACCGGTCGCC ACCATGGTGA
  121 GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG TGCCCATCCT GGTCGAGCTG GACGGCGACG
  181 TAAACGGCCA CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG CGATGCCACC TACGGCAAGC
  241 TGACCCTGAA GTTCATCTGC ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA
  301 CCACCCTGAC CTACGGCGTG CAGTGCTTCA GCCGCTACCC CGACCACATG AAGCAGCACG
  361 ACTTCTTCAA GTCCGCCATG CCCGAAGGCT ACGTCCAGGA GCGCACCATC TTCTTCAAGG
  421 ACGACGGCAA CTACAAGACC CGCGCCGAGG TGAAGTTCGA GGGCGACACC CTGGTGAACC
  481 GCATCGAGCT GAAGGGCATC GACTTCAAGG AGGACGGCAA CATCCTGGGG CACAAGCTGG
  541 AGTACAACTA CAACAGCCAC AACGTCTATA TCATGGCCGA CAAGCAGAAG AACGGCATCA
  601 AGGTGAACTT CAAGATCCGC CACAACATCG AGGACGGCAG CGTGCAGCTC GCCGACCACT
  661 ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT GCCCGACAAC CACTACCTGA
  721 GCACCCAGTC CGCCCTGAGC AAAGACCCCA ACGAGAAGCG CGATCACATG GTCCTGCTGG
  781 AGTTCGTGAC CGCCGCCGGG ATCACTCTCG GCATGGACGA GCTGTACAAG TCCGGACTCA
  841 GATCTATGAC TGAGGACTCT CAGAGAAACT TTCGTTCAGT ATATTATGAG AAAGTGGGGT
  901 TTCGTGGAGT TGAAGAAAAG AATCATTAGA AATTCTCCTA AAAGATGACC GTCTGGATAC
  961 TGAGAAACTT TTGTACTTTT TAGTCAGAGG TTTCCCTCTC CCGTCCCATG TACCGGTGCA
 1021 TTGGTATGGA AAGGTGCTTC TAAGATCTTG CTCCACACAC GAGTTCCATG CCAAGGTTGA
1081 ATGAATGTAT CGTTAAGGC
//
Primer: f1ori_R3 ()
Sequence file: RDB14989_A7Egd.seq check
>D04556C1_1_f1ori_R3_D01_10_ABI24.ab1
    1 TCCAGAAGCC ATTTTGGGGG ATGTGCGCGG ACCCCTATTT GTTTATTTTT CTAAATACAT
   61 TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA
  121 AGGAAGAGTC CTGAGGCGGA AAGAACCAGC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA
  181 AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA
  241 CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA
  301 ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA
  361 GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG
  421 CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT
  481 TTTGCAAAGA TCGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA
  541 TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA
  601 CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT
  661 CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAAGACGA GGCAGCGCGG
  721 CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA
  781 GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC
  841 CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT
  901 GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT
  961 CGGATGGAAG CCGGGTCTTG TCGATCAGAT GATCTGGACG AGAGCATCAT GGCTCGCGCC
 1021 AGCGAACTGT TCGCAGCTCA AGCGAGCATG CCCGACGCCG AAGAATCTCG TTCGTGACCA
1081 ATGCGATGCC TGCTTGCGAA TATCATGTTG AAATGGACGC TTTTTCG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ishibashi, K., Identification and characterization of a novel Tre-2/Bub2/Cdc16 (TBC) protein that possesses Rab3A-GAP activity. Genes Cells 14 (1): 41-52 (2009). PMID 19077034. [link to RRC of NBRP]