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pEGFP-C1-human TBC1D2A

Expression vector of human TBC1 domain family member 2 (TBC1D2), fused with N-terminal EGFP, CMV promoter.

Catalog number RDB14983
Resource name pEGFP-C1-human TBC1D2A
Alternative name FLJ22452; TBC1D2
Clone info. Expression vector of human TBC1 domain family member 2 (TBC1D2), fused with N-terminal EGFP, CMV promoter.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name human TBC1D2 cDNA
Reference of insert sequence AB449882 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
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External Database
human TBC1D2

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Itoh, T. and Fukuda, M., J. Biol. Chem., 281(42): 31823-31831, 2006)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Itoh, T. and Fukuda, M., J. Biol. Chem., 281(42): 31823-31831, 2006)。
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          RDB14983 pEGFP-C1-human TBC1D2A DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          original Itoh, T., Identification of EPI64 as a GTPase-activating protein specific for Rab27A. J. Biol. Chem. 281 (42): 31823-31831 (2006). PMID 16923811.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14983_A7Egp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EGFP-C2 (Pr0402)
          Region: EGFP3',insert5'
          Sequence file: RDB14983_A7Ega.seq check
          >D04556B4_A7Eg_1_EGFP-C2_E01_13_ABI24.ab1
              1 CGAACCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT TCGTGACCGC CGCCGGGATC
             61 ACTCTCGGCA TGGACGAGCT GTACAAGTCC GGACTCAGAT CTATGGAGGG CGCTGGGGAG
            121 AACGCCCCGG AGTCCAGCTC CTCTGCCCCT GGGTCCGAAG AGTCTGCCAG GGATCCACAG
            181 GTGCCGCCTC CGGAGGAAGA ATCGGGGGAC TGCGCCCGGT CCCTGGAGGC GGTCCCCAAG
            241 AAACTCTGTG GGTATTTAAG TAAGTTCGGC GGCAAAGGGC CCATCCGGGG CTGGAAATCC
            301 CGCTGGTTCT TCTACGACGA AAGGAAATGT CAGCTGTATT ACTCGCGGAC CGCTCAGGAT
            361 GCCAATCCCT TGGACAGCAT CGACCTCTCC AGTGCAGTGT TTGACTGTAA GGCGGACGCT
            421 GAGGAGGGGA TCTTCGAAAT CAAGACTCCC AGCCGGGTTA TTACCCTGAA GGCCGCCACC
            481 AAGCAAGCGA TGCTGTACTG GCTGCAGCAG CTGCAGATGA AGCGCTGGGA ATTCCACAAC
            541 AGCCCGCCGG CACCTCCTGC CACCCCTGAT GCCGCCCTGG CTGGGAATGG GCCCGTCCTG
            601 CACCTCGAGC TAGGGCAAGA AGAGGCAGAG CTGGAGGAGT TCCTGTGCCC TGTGAAAACA
            661 CCCCCTGGGC TAGTGGGCGT GGCAGCTGCC TTGCAGCCCT TCCCTGCCCT TCAGAATATT
            721 TCCCTCAAGC ACCTGGGGAC TGAAATACAG AACACAATGC ACAACATCCG TGGCAACAAG
            781 CAGGCCCAGG GAACAGGCCA TGAACCTCCA GGGGAAGATT CTCCACAGAG TGGGGAGCCT
            841 CAGAGGGAGG AGCAGCCCTT GGCCTCTGAC GCCAGCACCC CAGGGAGAGA GCCAGAGGAT
            901 TCTCCAAAGC CTGCACCCAA GCCCTCTCTG ACCATCAGTT TCGCTCAGAA AGCCAAGCGC
            961 CAGACAACAC CTTCCCATTC TTTTCTGAAG GAATCACACG GGAACCCGAA CTGCCCAGGA
           1021 GAAAGTGGCA GCCTTGGAGC AACAGCTCTG ATGCTCACCA AGGAGTAAGT CTCAGAGAGC
           1081 TAGTGAAGAT CCTTGCACAG GCACTGAAGT GCCCAGCCAG AGAAGGCGGG GCGGTCCAGC
          1141 CGCCCAATAC
          //
          Primer: SV40polyA_R2 (Pr0623)
          Region: SV40p(A),insert3'
          Sequence file: RDB14983_A7Egb.seq check
          >D04556B4_A7Eg_1_SV40polyA-R2_D02_11_ABI24.ab1
              1 CTTTATAAGC TGCATAAACA AGTTAACAAC AACAATTGCA TTCATTTTAT GTTTCAGGTT
             61 CAGGGGGAGG TGTGGGAGGT TTTTTAAAGC AAGTAAAACC TCTACAAATG TGGTATGGCT
            121 GATTATGATC AGTTATCTAG ATCCGGTGGA TCCCGGGCCC GCGGTACCGT CGACTGCAGA
            181 ATTCGATTTC AGGCTTCCCC CTCCACCTCG TCCTCGCTGG CACAGCCCTC GGACACAGCT
            241 CTGCGCCGGG ATGCCCGCCT CTCCAGGTAC TCTGCCTTAA GCTGCTCCAG CTCCCGCAGC
            301 TCAGCCTCCA GCCGCTCCCG GTGGACCATG CGCAGCTGCC GCAGCTGTTT CATGCGGAAG
            361 GGGTTCATGT CATTGAAGGC GATGTTCATC AGCTTCCGGC TGTTGGAGAT GGTCTTGGTG
            421 AAGAAGCGCA GGTACTGGTA GATTTCCAGG CCATTCTGTA GCCTCAAGAT CTCCTTCTCG
            481 TTGTACTTGA AAATGGCCAA GGCATAGCGA AACACCACCT TCGTCCCCTC GTACAGGAAG
            541 GCATCCCAGA CCCGAAGGAG GATGTTGCTA ATGAGACTGT CCGCAAAGAC CACGAGGAAC
            601 CAGTTGAAGG TGACGAGGGA GAGATCCACG TGGTGCTGCC CCAGATGGGC CATCAGCCTG
            661 GGCAGCTTCT CCGAGAGCAG GTCCTGGAGC ACCCGCTGGT CCACCTGGGA TGCCGTCAGC
            721 GTGTTGCAGT AGTAATCAGC GGGCATGATG GTCTCCACAA TGGCCACCAG GCACCAGAAG
            781 GCGCTCTCCT CCTCCTCTAG GACCAGCAGG GCAATGGCCG CCAGCCTGTT CAGGCCCTGG
            841 CAGTAGCCGA TGGTGGGGTT CTGCCAGGAG AAGGCCAGCA GCACCCGGCG GAGCTTGTCG
            901 GGGAAGCTGG AGGTGGGGCA GTGAAGTGTT TGTTGTTGGG GAAAGGTCCC GGTTCAGTCC
            961 AGCTCAATCT GGCGGGCAGC AGGTGCTCGC GGGCCTGGGC CCCGGCTCAG CAGTTCCTGG
           1021 TAGCAGCCTG GAGTGTGCAG TGCTGGGACA CGGAGGTGGG ACCAGCCACC TCCAGAACAC
           1081 GAGCTGTGTT CACGGGGTAC TCTGCCGCAG TAGCCTGCCT TGAGCTCGCC TGAGGCACAC
          1141 AAAGAATTCG GCCCC
          //
          Primer: CMV_Forward (Pr0016)
          Region: CMV pro,EGFP
          Sequence file: RDB14983_A7Egc.seq check
          >D04556B4_A7Eg_1_CMV_Forward_C02_08_ABI24.ab1
              1 CTTCCTCGCC CATTGACGCA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA
             61 GAGCTGGTTT AGTGAACCGT CAGATCCGCT AGCGCTACCG GTCGCCACCA TGGTGAGCAA
            121 GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA
            181 CGGCCACAAG TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC
            241 CCTGAAGTTC ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC
            301 CCTGACCTAC GGCGTGCAGT GCTTCAGCCG CTACCCCGAC CACATGAAGC AGCACGACTT
            361 CTTCAAGTCC GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT TCAAGGACGA
            421 CGGCAACTAC AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT
            481 CGAGCTGAAG GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA
            541 CAACTACAAC AGCCACAACG TCTATATCAT GGCCGACAAG CAGAAGAACG GCATCAAGGT
            601 GAACTTCAAG ATCCGCCACA ACATCGAGGA CGGCAGCGTG CAGCTCGCCG ACCACTACCA
            661 GCAGAACACC CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGAGCAC
            721 CCAGTCCGCC CTGAGCAAAG ACCCCAACGA GAAGCGCGAT CACATGGTCC TGCTGGAGTT
            781 CGTGACCGCC GCCGGGATCA CTCTCGGCAT GGACGAGCTG TACAGTCCGA CTCAGATCTA
            841 TGGAGGCGCT GGGGAGACGC CCCGGAGTCC AGCTCCTCTG CCCTGGGTCG AGAGTCTGCA
            901 AGATCACAGT GCCGCCTCGA GAGGATCGGG GACTGCCCCG TCCTTGAGCC GTTCCAGAAA
            961 CTCTGTGGGT ATTCAGTTAG TTCGCGCAGT CCATTCAGGC TGGATTCCGC TGATCTCCCA
          1021 GACTAGAAGT CACGTTATAT CGGACGCTGA TGCATCCGTG GACGCAATG
          //
          Primer: SV40pro_F_V2 (Pr0154)
          Region: SV40 pro_ori,NeoR_KanR
          Sequence file: RDB14983_A7Egd.seq check
          >D04556B4_A7Eg_1_SV40pro_F_V2_G06_21_ABI24.ab1
              1 TACAACCTTT TTTGGAGGCT AGGCTTTTGC AAGATCGATC AAGAGACAGG ATGAGGATCG
             61 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG
            121 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG
            181 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT
            241 GAACTGCAAG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA
            301 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG
            361 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT
            421 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA
            481 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG
            541 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGAGCATG
            601 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG
            661 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT
            721 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC
            781 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC
            841 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC
            901 CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG GTTGGGCTTC
            961 GGAAATCGTT TTCCCGGGAC GCCCGGCTGG GATGATCCCT CCCAGCGCGG GGATCTCATG
           1021 CTGGGAGTTC TTTCGCCACC CTAGGGGAGG CTAACTGAAC ACGATGAGAC ATACCCGGAG
          1081 GACCGCGCTA TGACCGGCAA TAAAAAGAAC AGCATTAAAA ACGGCCACG
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Itoh, T., Identification of EPI64 as a GTPase-activating protein specific for Rab27A. J. Biol. Chem. 281 (42): 31823-31831 (2006). PMID 16923811.
          reference Itoh, T., Screening for target Rabs of TBC (Tre-2/Bub2/Cdc16) domain-containing proteins based on their Rab-binding activity. Genes Cells 11 (9): 1023-1037 (2006). PMID 16923123.

          2023.04.10

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