Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEGFP-C1-human TBC1D2A (#RDB14983)

Expression vector of human TBC1 domain family member 2 (TBC1D2), fused with N-terminal EGFP, CMV promoter.

Alternative name FLJ22452; TBC1D2
Clone info. Expression vector of human TBC1 domain family member 2 (TBC1D2), fused with N-terminal EGFP, CMV promoter.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name human TBC1D2 cDNA
Reference of insert sequence AB449882 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Itoh, T. and Fukuda, M., J. Biol. Chem., 281(42): 31823-31831, 2006)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Itoh, T. and Fukuda, M., J. Biol. Chem., 281(42): 31823-31831, 2006)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14983 pEGFP-C1-human TBC1D2A DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-human TBC1D2A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14983).

Reference section:

Itoh, T., Fukuda, M., Identification of EPI64 as a GTPase-activating protein specific for Rab27A. J. Biol. Chem. 281 (42): 31823-31831 (2006). PMID 16923811. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14983_A7Egp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 (Pr0402)
Region: EGFP3',insert5'
Sequence file: RDB14983_A7Ega.seq check
>D04556B4_A7Eg_1_EGFP-C2_E01_13_ABI24.ab1
    1 CGAACCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT TCGTGACCGC CGCCGGGATC
   61 ACTCTCGGCA TGGACGAGCT GTACAAGTCC GGACTCAGAT CTATGGAGGG CGCTGGGGAG
  121 AACGCCCCGG AGTCCAGCTC CTCTGCCCCT GGGTCCGAAG AGTCTGCCAG GGATCCACAG
  181 GTGCCGCCTC CGGAGGAAGA ATCGGGGGAC TGCGCCCGGT CCCTGGAGGC GGTCCCCAAG
  241 AAACTCTGTG GGTATTTAAG TAAGTTCGGC GGCAAAGGGC CCATCCGGGG CTGGAAATCC
  301 CGCTGGTTCT TCTACGACGA AAGGAAATGT CAGCTGTATT ACTCGCGGAC CGCTCAGGAT
  361 GCCAATCCCT TGGACAGCAT CGACCTCTCC AGTGCAGTGT TTGACTGTAA GGCGGACGCT
  421 GAGGAGGGGA TCTTCGAAAT CAAGACTCCC AGCCGGGTTA TTACCCTGAA GGCCGCCACC
  481 AAGCAAGCGA TGCTGTACTG GCTGCAGCAG CTGCAGATGA AGCGCTGGGA ATTCCACAAC
  541 AGCCCGCCGG CACCTCCTGC CACCCCTGAT GCCGCCCTGG CTGGGAATGG GCCCGTCCTG
  601 CACCTCGAGC TAGGGCAAGA AGAGGCAGAG CTGGAGGAGT TCCTGTGCCC TGTGAAAACA
  661 CCCCCTGGGC TAGTGGGCGT GGCAGCTGCC TTGCAGCCCT TCCCTGCCCT TCAGAATATT
  721 TCCCTCAAGC ACCTGGGGAC TGAAATACAG AACACAATGC ACAACATCCG TGGCAACAAG
  781 CAGGCCCAGG GAACAGGCCA TGAACCTCCA GGGGAAGATT CTCCACAGAG TGGGGAGCCT
  841 CAGAGGGAGG AGCAGCCCTT GGCCTCTGAC GCCAGCACCC CAGGGAGAGA GCCAGAGGAT
  901 TCTCCAAAGC CTGCACCCAA GCCCTCTCTG ACCATCAGTT TCGCTCAGAA AGCCAAGCGC
  961 CAGACAACAC CTTCCCATTC TTTTCTGAAG GAATCACACG GGAACCCGAA CTGCCCAGGA
 1021 GAAAGTGGCA GCCTTGGAGC AACAGCTCTG ATGCTCACCA AGGAGTAAGT CTCAGAGAGC
 1081 TAGTGAAGAT CCTTGCACAG GCACTGAAGT GCCCAGCCAG AGAAGGCGGG GCGGTCCAGC
1141 CGCCCAATAC
//
Primer: SV40polyA_R2 (Pr0623)
Region: SV40p(A),insert3'
Sequence file: RDB14983_A7Egb.seq check
>D04556B4_A7Eg_1_SV40polyA-R2_D02_11_ABI24.ab1
    1 CTTTATAAGC TGCATAAACA AGTTAACAAC AACAATTGCA TTCATTTTAT GTTTCAGGTT
   61 CAGGGGGAGG TGTGGGAGGT TTTTTAAAGC AAGTAAAACC TCTACAAATG TGGTATGGCT
  121 GATTATGATC AGTTATCTAG ATCCGGTGGA TCCCGGGCCC GCGGTACCGT CGACTGCAGA
  181 ATTCGATTTC AGGCTTCCCC CTCCACCTCG TCCTCGCTGG CACAGCCCTC GGACACAGCT
  241 CTGCGCCGGG ATGCCCGCCT CTCCAGGTAC TCTGCCTTAA GCTGCTCCAG CTCCCGCAGC
  301 TCAGCCTCCA GCCGCTCCCG GTGGACCATG CGCAGCTGCC GCAGCTGTTT CATGCGGAAG
  361 GGGTTCATGT CATTGAAGGC GATGTTCATC AGCTTCCGGC TGTTGGAGAT GGTCTTGGTG
  421 AAGAAGCGCA GGTACTGGTA GATTTCCAGG CCATTCTGTA GCCTCAAGAT CTCCTTCTCG
  481 TTGTACTTGA AAATGGCCAA GGCATAGCGA AACACCACCT TCGTCCCCTC GTACAGGAAG
  541 GCATCCCAGA CCCGAAGGAG GATGTTGCTA ATGAGACTGT CCGCAAAGAC CACGAGGAAC
  601 CAGTTGAAGG TGACGAGGGA GAGATCCACG TGGTGCTGCC CCAGATGGGC CATCAGCCTG
  661 GGCAGCTTCT CCGAGAGCAG GTCCTGGAGC ACCCGCTGGT CCACCTGGGA TGCCGTCAGC
  721 GTGTTGCAGT AGTAATCAGC GGGCATGATG GTCTCCACAA TGGCCACCAG GCACCAGAAG
  781 GCGCTCTCCT CCTCCTCTAG GACCAGCAGG GCAATGGCCG CCAGCCTGTT CAGGCCCTGG
  841 CAGTAGCCGA TGGTGGGGTT CTGCCAGGAG AAGGCCAGCA GCACCCGGCG GAGCTTGTCG
  901 GGGAAGCTGG AGGTGGGGCA GTGAAGTGTT TGTTGTTGGG GAAAGGTCCC GGTTCAGTCC
  961 AGCTCAATCT GGCGGGCAGC AGGTGCTCGC GGGCCTGGGC CCCGGCTCAG CAGTTCCTGG
 1021 TAGCAGCCTG GAGTGTGCAG TGCTGGGACA CGGAGGTGGG ACCAGCCACC TCCAGAACAC
 1081 GAGCTGTGTT CACGGGGTAC TCTGCCGCAG TAGCCTGCCT TGAGCTCGCC TGAGGCACAC
1141 AAAGAATTCG GCCCC
//
Primer: CMV_Forward (Pr0016)
Region: CMV pro,EGFP
Sequence file: RDB14983_A7Egc.seq check
>D04556B4_A7Eg_1_CMV_Forward_C02_08_ABI24.ab1
    1 CTTCCTCGCC CATTGACGCA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA
   61 GAGCTGGTTT AGTGAACCGT CAGATCCGCT AGCGCTACCG GTCGCCACCA TGGTGAGCAA
  121 GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA
  181 CGGCCACAAG TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC
  241 CCTGAAGTTC ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC
  301 CCTGACCTAC GGCGTGCAGT GCTTCAGCCG CTACCCCGAC CACATGAAGC AGCACGACTT
  361 CTTCAAGTCC GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT TCAAGGACGA
  421 CGGCAACTAC AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT
  481 CGAGCTGAAG GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA
  541 CAACTACAAC AGCCACAACG TCTATATCAT GGCCGACAAG CAGAAGAACG GCATCAAGGT
  601 GAACTTCAAG ATCCGCCACA ACATCGAGGA CGGCAGCGTG CAGCTCGCCG ACCACTACCA
  661 GCAGAACACC CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGAGCAC
  721 CCAGTCCGCC CTGAGCAAAG ACCCCAACGA GAAGCGCGAT CACATGGTCC TGCTGGAGTT
  781 CGTGACCGCC GCCGGGATCA CTCTCGGCAT GGACGAGCTG TACAGTCCGA CTCAGATCTA
  841 TGGAGGCGCT GGGGAGACGC CCCGGAGTCC AGCTCCTCTG CCCTGGGTCG AGAGTCTGCA
  901 AGATCACAGT GCCGCCTCGA GAGGATCGGG GACTGCCCCG TCCTTGAGCC GTTCCAGAAA
  961 CTCTGTGGGT ATTCAGTTAG TTCGCGCAGT CCATTCAGGC TGGATTCCGC TGATCTCCCA
1021 GACTAGAAGT CACGTTATAT CGGACGCTGA TGCATCCGTG GACGCAATG
//
Primer: SV40pro_F_V2 (Pr0154)
Region: SV40 pro_ori,NeoR_KanR
Sequence file: RDB14983_A7Egd.seq check
>D04556B4_A7Eg_1_SV40pro_F_V2_G06_21_ABI24.ab1
    1 TACAACCTTT TTTGGAGGCT AGGCTTTTGC AAGATCGATC AAGAGACAGG ATGAGGATCG
   61 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG
  121 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG
  181 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT
  241 GAACTGCAAG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA
  301 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG
  361 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT
  421 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA
  481 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG
  541 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGAGCATG
  601 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG
  661 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT
  721 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC
  781 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC
  841 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC
  901 CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG GTTGGGCTTC
  961 GGAAATCGTT TTCCCGGGAC GCCCGGCTGG GATGATCCCT CCCAGCGCGG GGATCTCATG
 1021 CTGGGAGTTC TTTCGCCACC CTAGGGGAGG CTAACTGAAC ACGATGAGAC ATACCCGGAG
1081 GACCGCGCTA TGACCGGCAA TAAAAAGAAC AGCATTAAAA ACGGCCACG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Itoh, T., Identification of EPI64 as a GTPase-activating protein specific for Rab27A. J. Biol. Chem. 281 (42): 31823-31831 (2006). PMID 16923811. [link to RRC of NBRP]
reference Itoh, T., Screening for target Rabs of TBC (Tre-2/Bub2/Cdc16) domain-containing proteins based on their Rab-binding activity. Genes Cells 11 (9): 1023-1037 (2006). PMID 16923123. [link to RRC of NBRP]