RDB14690 - pEF-T7-Syt X (Syt10)

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pEF-T7-Syt X (Syt10) (#RDB14690)

Expression vector of mouse synaptotagmin X (Syt X) [Syt 10].

Clone info.	Expression vector of mouse synaptotagmin X (Syt X) [Syt 10], T7-tag, EF1 promoter.
Comment	Expression was confirmed by the depositor with immunoprecipitation.
Vector backbone	pEF-T7 (plasmid)
Selectable markers	Amp^r
Gene/insert name	mouse Syt10 cDNA
Reference of insert sequence	AB026807 (DDBJ accession number)
Depositor\|Developer	Fukuda, Mitsunori \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Fukuda, M. et al. J. Biol. Chem. 274, 31421-31427, 1999)
Additional terms and conditions:
Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Fukuda, M. et al. J. Biol. Chem. 274, 31421-31427, 1999) Additional terms and conditions: Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Fukuda, M. et al. J. Biol. Chem. 274, 31421-31427, 1999)。 MTAに書く付加的使用条件： pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。

必要書類

提供依頼書

[依頼書の記入例

]
提供同意書 (MTA, 非営利学術目的用)[Word

] [MTAの記入例

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。

MTAに書く使用条件

利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Fukuda, M. et al. J. Biol. Chem. 274, 31421-31427, 1999)。
MTAに書く付加的使用条件：
pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB14690	pEF-T7-Syt X (Syt10)	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEF-T7-Syt X (Syt10) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14690).

Reference section:

Fukuda, M., Kanno, E., Mikoshiba, K., Conserved N-terminal cysteine motif is essential for homo- and heterodimer formation of synaptotagmins III, V, VI, and X. J. Biol. Chem. 274 (44): 31421-31427 (1999). PMID 10531343. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB14690_A7Ddp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F Sequence file: RDB14690_A7Dda.seq
>D04472B8_A7Dd_1_EF1a-C_F_H06_24_ABI24.ab1 1 CCATCATGCC TTTTTGAGTT TGGATCTTGG TTCATTCTCA AGCCTCAGAC AGTGGTTCAA 61 AGTTTTTTTC TTCCATTTCA GGTGTCGTGA GGAATTCTCT AGAGCGGCCG CGGGAATTCG 121 ATTCCACCAT GGCTAGCATG ACTGGTGGAC AGCAAATGGG TCGCGGATCC ATGAGTTTCC 181 GCAAGGAGGA CGGTGTGAGC AGCCTGTGCC AGAAGGCGCT GCACATCATC ACCGAGCTGT 241 GCTTCGCGGG TCAGGTGGAG TGGGACAAGT GTTCGGGCAT CTTTCCAGCC GATAGGAGCG 301 GCCAGGGGGG AGGTGGCACA GATATTTCAG TCAGCCTGTT AGCGGTTGTT GTAAGCTTCT 361 GTGGCCTGGC CTTGCTGGTT GTCTCCCTTT TTGTCTTCTG GAAGTTGTGC TGGCCATGCT 421 GGAAGAGCAA GCTTGTGGCC CCCAACCTCA GTGTCCTTCC ACAAAGCATC TCAAGTGCTC 481 CAACTGAAGT TTTTGAGACT GAAGAGAAAA AAGAAGTGGA AGAAAATGAA AAGCCAGCAC 541 CCAAAGCCAT TGAGCCTGCA ATAAAAATCA GCCACACATC CCCCGACATC CCAGCAGAAG 601 TGCAGACAGC TTTAAAGGAG CACTTGATTA AACATGCTCG AGTGCAAAGG CAAACAACCG 661 AGCCTACGTC TTCATCCCGC CACAACTCCT TCAGGAGACA CCTACCAAGG CAAATGAATG 721 TCTCCAGCGT CGACTTTAGC GTGGGCACTG AACCCATCTT ACAAAGAGGA GAAACGAGAA 781 CGAGCATCGG GAGGATAAAA CCAGAACTCT ACAAGCAGAA ATCGGTTGAC TCCGAGGGCA 841 ACAGAAAAGA CGACGTCAAG ACCTGTGGGA AACTTAACTT TGCGCTCCAG TATGATTATG 901 AAAATGAAAC TCCTAGTTGT TAAAATTATC AAAGCTTTAG ATCTCCCAGC TAAAGATTTC 961 ACAGGAAACT TCTGATCCCT TATGTGAAGA TCCTATCCTT TCTTTCCCGG ATAGGGAGAG 1021 AAATTTCAGA CCCGCGTGCA CCGAGACCTT TAAACCCTCT GTTTGATGAG CTAATTTCCA 1081 GTTTCCGGGT GGATTACCGG ATC //
Primer: hGCSF-R Sequence file: RDB14690_A7Ddb.seq
>D04472B8_A7Dd_1_hGCSF_R_H07_22_ABI24.ab1 1 GGGGGGAATG ATCTCTCAAG GGTCCCAGGT GGGGACCCTC ACTCTAGAGC GGCCGCGAAT 61 TCACTAGTGG ATCCCCCGGG CTGCAGGAAT TCGATTCTTT CTGTGATATG GTCTTATTTG 121 GAGGCTTTTA TGGTGTGGAC GGTGGCCTGG GAGAAGAACA GGACCCTTGA CTATCAAAGC 181 TGGTGGCCCG GCCAGGTAAC TCCAGCAAGG GGTGCCAGTG TGTGATTGGC TTTCGGTGAT 241 AGGCCAGCAT CTCGTTCCAA TGGTCTCTCC CAAGACCCTC AGCGTCTAGT CCAGTTCGAC 301 ACACGCCTAT GACCTCATTG TGCCCCACCC TGTCGTAATC CATGACTGCA ATGCACAGGC 361 TGACCTGGTC CACATTCTCT GGAGGGATAT CGAAGATAAT GGCTTCGTTG TACACGGGAT 421 TGAGAGTATT TTTCTTTGTA GTTGTTTTCC GTTTTTTAAG TCTTCGGCCC TCACACATCA 481 GAGACACTTT GACATAAGGA TCTGATGACC CAGTGATGTC CATTGCCTTC AGATTTCTGC 541 ACTTGATCAC TGTCAAGGTC ATACGGCCAG CAGTTGGCAA ATAGCAAAGG GAGAACATGA 601 TTTCCCCCAG GTCTATGCTT TCTGTGGTAG CACAGTGGAT ATCTTTCCAT ACTGTGGCTT 661 CCCTGGAGAG ATCAGAGACT TCAAATAAAT TATCAAGAAT CACTTCCCCA ATCATGTCGT 721 GTCTGGAAAA TCTGTCAAAA TCATATATAC TAAAGTGTAG TTTGCGGTTG CTTAGCTGAT 781 CGTAAACCAC CGGAAACTGA AATAGCTCAT CAAACAGAGG GTTTAAAGTC TTCCGGTGCA 841 CGCGGGTCTG AAATTTCTTC TTCCTATCCG GAAGAAGATA GATCTTCACA TAAAGGATCA 901 GAAGTTCCTG TGAAATCTTT AGCTGGGAGA TCTAAAGCTT TGATAATTTT AACAACTAGG 961 GAGTTCATTT TCATAATCAT ACTGGAGCGC AAGTTTAAGT TTCCACAGTC CTTGACGTCG 1021 TCCTTTTTCT GTTGCCCTCG GAGTCACCGA TTTCTGCCTG TAAGTCCTGG ATTTATCTCC 1081 GATGCTCGGA TTCTCGGTTT CTCCTCTTTG GTGAGAATGA GTTCG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Fukuda, M., Conserved N-terminal cysteine motif is essential for homo- and heterodimer formation of synaptotagmins III, V, VI, and X. J. Biol. Chem. 274 (44): 31421-31427 (1999). PMID 10531343. [link to RRC of NBRP]

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mouse Syt10

External Database
mouse Syt10

Reference sequence