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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEF-T7-Syt X (Syt10)

Expression vector of mouse synaptotagmin X (Syt X) [Syt 10].

Catalog number RDB14690
Resource name pEF-T7-Syt X (Syt10)
Clone info. Expression vector of mouse synaptotagmin X (Syt X) [Syt 10], T7-tag, EF1 promoter.
Expression was confirmed by the depositor with immunoprecipitation.
Vector backbone pEF-T7 (plasmid)
Selectable markers Amp^r
Gene/insert name mouse Syt10 cDNA
Reference of nucleotide sequence AB026807 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
 
Other clones in our bank mouse Syt10 (NCBI Gene 54526) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Fukuda, M. et al. J. Biol. Chem. 274, 31421-31427, 1999)
Additional terms and conditions for distribution Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14690 pEF-T7-Syt X (Syt10) DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Fukuda, M., Conserved N-terminal cysteine motif is essential for homo- and heterodimer formation of synaptotagmins III, V, VI, and X. J. Biol. Chem. 274 (44): 31421-31427 (1999). PMID 10531343.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14690_A7Ddp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F
Sequence file: RDB14690_A7Dda.seq check
>D04472B8_A7Dd_1_EF1a-C_F_H06_24_ABI24.ab1
    1 CCATCATGCC TTTTTGAGTT TGGATCTTGG TTCATTCTCA AGCCTCAGAC AGTGGTTCAA
   61 AGTTTTTTTC TTCCATTTCA GGTGTCGTGA GGAATTCTCT AGAGCGGCCG CGGGAATTCG
  121 ATTCCACCAT GGCTAGCATG ACTGGTGGAC AGCAAATGGG TCGCGGATCC ATGAGTTTCC
  181 GCAAGGAGGA CGGTGTGAGC AGCCTGTGCC AGAAGGCGCT GCACATCATC ACCGAGCTGT
  241 GCTTCGCGGG TCAGGTGGAG TGGGACAAGT GTTCGGGCAT CTTTCCAGCC GATAGGAGCG
  301 GCCAGGGGGG AGGTGGCACA GATATTTCAG TCAGCCTGTT AGCGGTTGTT GTAAGCTTCT
  361 GTGGCCTGGC CTTGCTGGTT GTCTCCCTTT TTGTCTTCTG GAAGTTGTGC TGGCCATGCT
  421 GGAAGAGCAA GCTTGTGGCC CCCAACCTCA GTGTCCTTCC ACAAAGCATC TCAAGTGCTC
  481 CAACTGAAGT TTTTGAGACT GAAGAGAAAA AAGAAGTGGA AGAAAATGAA AAGCCAGCAC
  541 CCAAAGCCAT TGAGCCTGCA ATAAAAATCA GCCACACATC CCCCGACATC CCAGCAGAAG
  601 TGCAGACAGC TTTAAAGGAG CACTTGATTA AACATGCTCG AGTGCAAAGG CAAACAACCG
  661 AGCCTACGTC TTCATCCCGC CACAACTCCT TCAGGAGACA CCTACCAAGG CAAATGAATG
  721 TCTCCAGCGT CGACTTTAGC GTGGGCACTG AACCCATCTT ACAAAGAGGA GAAACGAGAA
  781 CGAGCATCGG GAGGATAAAA CCAGAACTCT ACAAGCAGAA ATCGGTTGAC TCCGAGGGCA
  841 ACAGAAAAGA CGACGTCAAG ACCTGTGGGA AACTTAACTT TGCGCTCCAG TATGATTATG
  901 AAAATGAAAC TCCTAGTTGT TAAAATTATC AAAGCTTTAG ATCTCCCAGC TAAAGATTTC
  961 ACAGGAAACT TCTGATCCCT TATGTGAAGA TCCTATCCTT TCTTTCCCGG ATAGGGAGAG
 1021 AAATTTCAGA CCCGCGTGCA CCGAGACCTT TAAACCCTCT GTTTGATGAG CTAATTTCCA
1081 GTTTCCGGGT GGATTACCGG ATC
//
Primer: hGCSF-R
Sequence file: RDB14690_A7Ddb.seq check
>D04472B8_A7Dd_1_hGCSF_R_H07_22_ABI24.ab1
    1 GGGGGGAATG ATCTCTCAAG GGTCCCAGGT GGGGACCCTC ACTCTAGAGC GGCCGCGAAT
   61 TCACTAGTGG ATCCCCCGGG CTGCAGGAAT TCGATTCTTT CTGTGATATG GTCTTATTTG
  121 GAGGCTTTTA TGGTGTGGAC GGTGGCCTGG GAGAAGAACA GGACCCTTGA CTATCAAAGC
  181 TGGTGGCCCG GCCAGGTAAC TCCAGCAAGG GGTGCCAGTG TGTGATTGGC TTTCGGTGAT
  241 AGGCCAGCAT CTCGTTCCAA TGGTCTCTCC CAAGACCCTC AGCGTCTAGT CCAGTTCGAC
  301 ACACGCCTAT GACCTCATTG TGCCCCACCC TGTCGTAATC CATGACTGCA ATGCACAGGC
  361 TGACCTGGTC CACATTCTCT GGAGGGATAT CGAAGATAAT GGCTTCGTTG TACACGGGAT
  421 TGAGAGTATT TTTCTTTGTA GTTGTTTTCC GTTTTTTAAG TCTTCGGCCC TCACACATCA
  481 GAGACACTTT GACATAAGGA TCTGATGACC CAGTGATGTC CATTGCCTTC AGATTTCTGC
  541 ACTTGATCAC TGTCAAGGTC ATACGGCCAG CAGTTGGCAA ATAGCAAAGG GAGAACATGA
  601 TTTCCCCCAG GTCTATGCTT TCTGTGGTAG CACAGTGGAT ATCTTTCCAT ACTGTGGCTT
  661 CCCTGGAGAG ATCAGAGACT TCAAATAAAT TATCAAGAAT CACTTCCCCA ATCATGTCGT
  721 GTCTGGAAAA TCTGTCAAAA TCATATATAC TAAAGTGTAG TTTGCGGTTG CTTAGCTGAT
  781 CGTAAACCAC CGGAAACTGA AATAGCTCAT CAAACAGAGG GTTTAAAGTC TTCCGGTGCA
  841 CGCGGGTCTG AAATTTCTTC TTCCTATCCG GAAGAAGATA GATCTTCACA TAAAGGATCA
  901 GAAGTTCCTG TGAAATCTTT AGCTGGGAGA TCTAAAGCTT TGATAATTTT AACAACTAGG
  961 GAGTTCATTT TCATAATCAT ACTGGAGCGC AAGTTTAAGT TTCCACAGTC CTTGACGTCG
 1021 TCCTTTTTCT GTTGCCCTCG GAGTCACCGA TTTCTGCCTG TAAGTCCTGG ATTTATCTCC
1081 GATGCTCGGA TTCTCGGTTT CTCCTCTTTG GTGAGAATGA GTTCG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Fukuda, M., Conserved N-terminal cysteine motif is essential for homo- and heterodimer formation of synaptotagmins III, V, VI, and X. J. Biol. Chem. 274 (44): 31421-31427 (1999). PMID 10531343.

2021.03.26

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