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pEF-Tyrosinase-FLAG

Expression vector of mouse Tyrosinase with C-terminal FLAG-tag under the control of the EF1 alpha promoter.

Catalog number RDB14635
Resource name pEF-Tyrosinase-FLAG
Clone info. Expression vector of mouse Tyrosinase with C-terminal FLAG-tag under the control of the EF1 alpha promoter.
Please refer Kanno, E. et al. Traffic, 11 (4): 491-507, 2010 (PMID 20070612) for pEF-FLAG vector. Expression was confirmed by the deposotor with immunoprecipitation and microscopy.
Vector backbone pEF-FLAG (plasmid)
Size of vector backbone 5.7 kb
Selectable markers Amp^r
Gene/insert name Mouse Tyr cDNA
Depositor Fukuda, Mitsunori |
 
Other clones in our bank mouse Tyr (NCBI Gene 22173) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ishida, M. et al., J. Biochem. 161 (4): 323-326, 2017)
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14635 pEF-Tyrosinase-FLAG DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Ishida, M., M-INK, a novel tool for visualizing melanosomes and melanocores. J. Biochem. 161 (4): 323-326 (2017). PMID 28096452.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dnaconda's recommendation EXR0070e (English text)
Featured content Dnaconda's recommendation EXR0070j (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14635_A7C1p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F RDB14635_A7C1a.seq
>D04384A4_A7C1_2_EF1a-C_F_C03_09_ABI24.ab1
    1 TTATTTGCAT TGTCTTTTTG AGTTTGGATC TTGGTTCATT CTCAAGCCTC AGACAGTGGT
   61 TCAAAGTTTT TTTCTTCCAT TTCAGGTGTC GTGAGGAATT CTCTAGAGCG GCCGCTCTAG
  121 AACTAGTGGA TCCATGTTCT TGGCTGTTTT GTATTGCCTT CTGTGGAGTT TCCAGATCTC
  181 TGATGGCCAT TTTCCTCGAG CCTGTGCCTC CTCTAAGAAC TTGTTGGCAA AAGAATGCTG
  241 CCCACCATGG ATGGGTGATG GGAGTCCCTG CGGCCAGCTT TCAGGCAGAG GTTCCTGCCA
  301 GGATATCCTT CTGTCCAGTG CACCATCTGG ACCTCAGTTC CCCTTCAAAG GGGTGGATGA
  361 CCGTGAGTCC TGGCCCTCTG TGTTTTATAA TAGGACCTGC CAGTGCTCAG GCAACTTCAT
  421 GGGTTTCAAC TGCGGAAACT GTAAGTTTGG ATTTGGGGGC CCAAATTGTA CAGAGAAGCG
  481 AGTCTTGATT AGAAGAAACA TTTTTGATTT GAGTGTCTCC GAAAAGAATA AGTTCTTTTC
  541 TTACCTCACT TTAGCAAAAC ATACTATCAG CTCAGTCTAT GTCATCCCCA CAGGCACCTA
  601 TGGCCAAATG AACAATGGGT CAACACCCAT GTTTAATGAT ATCAACATCT ACGACCTCTT
  661 TGTATGGATG CATTACTATG TGTCAAGGGA CACACTGCTT GGGGGCTCTG AAATATGGAG
  721 GGACATTGAT TTTGCCCATG AAGCACCAGG GTTTCTGCCT TGGCACAGAC TTTTCTTGTT
  781 ATTGTGGGAA CAAGAAATTC GAGAACTAAC TGGGGATGAG AACTTCACTG TTCCATACTG
  841 GGATTGGAGA GATGCAGAAA ACTGTGACAT TTGCACAGAT GAGTACTTGG GAGGTCGTCA
  901 CCCTGAAAAT CCTAACTTAC TCAGCCCAGC ATCCTTCTTC TCCTCCTGGC AGATCATTTG
  961 TAGCAGATCA GAAGAGTATA ATAGCCATCA GGTTTTATGC GATGGAAACA CCCTGAGGGA
 1021 CCACTATTTA CGTAAATCCT GGAAAACCAT GACAAAAGCC CAAAACCCCC CAGGCTCCAT
 1081 CCTCCAGCAG ATGTGATTTG GTCCTGGATT GACCAGTATG AATCTGGAAT TCAAAAGTGG
//
Primer: hGCSF_R RDB14635_A7C1b.seq
>D04384A4_A7C1_2_hGCSF_R_D03_12_ABI24.ab1
    1 GTGGGGAAAA GGGATCTCTC AAGGGTCCCA GGGTGGGGAC CCTCACTCTA GAGCGGCCGC
   61 CTACTTGTCG TCATCGTCCT TGTAGTCCCG CGGGGTACCC AGATGGCTCT GATACAGCAA
  121 GCTGTGGTAG TCGTCTTTGT CCATGAGGAG TGGCTGCCTT TCCTCCTGGG GTTGCTTCTT
  181 CTTCTTCTTC TTCTGAAGGC ATAGCCTACT GCTAAGCCCA GAGAGAGCTG CAGCAATAAC
  241 AGCTCCCACC AGTGCTGCCC CAAGAAGCCA TGGCCAGATA CGACTGGCTT GTTCCAAGTA
  301 AGGCTCAATA TAATTTCTGT AAAAGCCTGG ATCTGACTCT TGGAGGTAGC TGTAGTCATA
  361 TCCCAGATCC TTGGATGTTA TGAAGAAATC ACCATTTCTA TAGAGCGGTA TGAAAGGAAC
  421 CATGTAAGAG TCTCTGTTAT GGCCGATAGG TGCATTGGCT TCTGGGTAAA CTTCCAAAAG
  481 AGGGCGGTGC CTTCGCAGCC ATTGTTCAAA AATACTGTCC ACAAAAGCAT GGTGAAGAAG
  541 AAAAATGGGA TCGTTGGCCG ATCCCTGTAC TTGGGACATT GTTCCATTCA TAAAGATATG
  601 TAAGGCATTG TGCATGCTAC TTTGAGAAGG ATCTGCTATC CCTGTGAGTG GACTGGCAAA
  661 TCCTTCCAGT GTGTTTCTAA AGCTGAAATT GGCAGTTCTA TCCATTGATC CAGATTCATA
  721 CTGGGTCAAA CTCAGACAAA ATTCCACATC TGCTGAAGAT GGGAGCCTGG GGGTTTTGGC
  781 TTTGTCATGG TTTCCAGGAT TACGTAATAG TGGTCCCTCA GTGTTCCATC GCATAAAACC
  841 TGATGGCTAT TATACTCTTC TGATCTGCTA CAAATGATCT GCCAGGAGGA GAAGAAGGAT
  901 GCTGGGCTGA GTAAGTTAGG ATTTTCAGGG TGACGACCTC CCAAGTACTC ATCTGTGCAA
  961 ATGTCACAGA TTTCTGCATC TCTCCCAATC CCAGTATGGA AACAGTGAAG TTTCTCATCC
 1021 CCAGTTTAGT TCTTCGAAAT TTCTTGTTCC CACCAATAAA CAAGAAAAAG TCTGTGCTAG
 1081 GCAGAAACCT GGGTGCTCAT GGCCAAAATT CCAATGTTCC CTCCATATTT TCAGAAAGCC
1141 CCCCCCCGAG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Ishida, M., M-INK, a novel tool for visualizing melanosomes and melanocores. J. Biochem. 161 (4): 323-326 (2017). PMID 28096452.

2019.05.23

GNP_filter3_RDBDEP_html_190430.pl