Expression vector of mouse Tyrosinase with C-terminal FLAG-tag under the control of the EF1 alpha promoter.
Clone info. | Expression vector of mouse Tyrosinase with C-terminal FLAG-tag under the control of the EF1 alpha promoter. |
---|---|
Comment | Please refer Kanno, E. et al. Traffic, 11 (4): 491-507, 2010 (PMID 20070612) for pEF-FLAG vector. Expression was confirmed by the deposotor with immunoprecipitation and microscopy. |
Vector backbone | pEF-FLAG (plasmid) |
Size of vector backbone | 5.7 kb |
Selectable markers | Amp^r |
Gene/insert name | Mouse Tyr cDNA |
Depositor|Developer | Fukuda, Mitsunori | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Information of Request for Distribution.[link] |
---|---|
Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ishida, M. et al., J. Biochem. 161 (4): 323-326, 2017) Additional terms and conditions: Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the pEF-BOS and pEF-BOS-EX vectors (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested. |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
---|---|
MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Ishida, M. et al., J. Biochem. 161 (4): 323-326, 2017)。 MTAに書く付加的使用条件: pEF-BOS and pEF-BOS-EXベクタについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクタを商業的に利用する場合、利用者は長田重一博士に連絡するものとする。利用者は、研究成果の公表にあたってpEF-BOSおよびpEF-BOS-EXベクタの文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990)を引用し、長田重一教授への謝辞の表明を必要とする。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
---|---|---|---|
RDB14635 | pEF-Tyrosinase-FLAG | DNA solution |
Materials & Methods section:
The pEF-Tyrosinase-FLAG was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14635). |
Reference section:
Ishida, M., Marubashi, S., Fukuda, M., M-INK, a novel tool for visualizing melanosomes and melanocores. J. Biochem. 161 (4): 323-326 (2017). PMID 28096452. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB14635_A7C1p1-1.pdf ![]() |
---|
Nucleotide sequence of a portion of this resource (if available).
Primer: EF1a-C_F () Sequence file: RDB14635_A7C1a.seq ![]() |
---|
>D04384A4_A7C1_2_EF1a-C_F_C03_09_ABI24.ab1 1 TTATTTGCAT TGTCTTTTTG AGTTTGGATC TTGGTTCATT CTCAAGCCTC AGACAGTGGT 61 TCAAAGTTTT TTTCTTCCAT TTCAGGTGTC GTGAGGAATT CTCTAGAGCG GCCGCTCTAG 121 AACTAGTGGA TCCATGTTCT TGGCTGTTTT GTATTGCCTT CTGTGGAGTT TCCAGATCTC 181 TGATGGCCAT TTTCCTCGAG CCTGTGCCTC CTCTAAGAAC TTGTTGGCAA AAGAATGCTG 241 CCCACCATGG ATGGGTGATG GGAGTCCCTG CGGCCAGCTT TCAGGCAGAG GTTCCTGCCA 301 GGATATCCTT CTGTCCAGTG CACCATCTGG ACCTCAGTTC CCCTTCAAAG GGGTGGATGA 361 CCGTGAGTCC TGGCCCTCTG TGTTTTATAA TAGGACCTGC CAGTGCTCAG GCAACTTCAT 421 GGGTTTCAAC TGCGGAAACT GTAAGTTTGG ATTTGGGGGC CCAAATTGTA CAGAGAAGCG 481 AGTCTTGATT AGAAGAAACA TTTTTGATTT GAGTGTCTCC GAAAAGAATA AGTTCTTTTC 541 TTACCTCACT TTAGCAAAAC ATACTATCAG CTCAGTCTAT GTCATCCCCA CAGGCACCTA 601 TGGCCAAATG AACAATGGGT CAACACCCAT GTTTAATGAT ATCAACATCT ACGACCTCTT 661 TGTATGGATG CATTACTATG TGTCAAGGGA CACACTGCTT GGGGGCTCTG AAATATGGAG 721 GGACATTGAT TTTGCCCATG AAGCACCAGG GTTTCTGCCT TGGCACAGAC TTTTCTTGTT 781 ATTGTGGGAA CAAGAAATTC GAGAACTAAC TGGGGATGAG AACTTCACTG TTCCATACTG 841 GGATTGGAGA GATGCAGAAA ACTGTGACAT TTGCACAGAT GAGTACTTGG GAGGTCGTCA 901 CCCTGAAAAT CCTAACTTAC TCAGCCCAGC ATCCTTCTTC TCCTCCTGGC AGATCATTTG 961 TAGCAGATCA GAAGAGTATA ATAGCCATCA GGTTTTATGC GATGGAAACA CCCTGAGGGA 1021 CCACTATTTA CGTAAATCCT GGAAAACCAT GACAAAAGCC CAAAACCCCC CAGGCTCCAT 1081 CCTCCAGCAG ATGTGATTTG GTCCTGGATT GACCAGTATG AATCTGGAAT TCAAAAGTGG // |
Primer: hGCSF_R () Sequence file: RDB14635_A7C1b.seq ![]() |
>D04384A4_A7C1_2_hGCSF_R_D03_12_ABI24.ab1 1 GTGGGGAAAA GGGATCTCTC AAGGGTCCCA GGGTGGGGAC CCTCACTCTA GAGCGGCCGC 61 CTACTTGTCG TCATCGTCCT TGTAGTCCCG CGGGGTACCC AGATGGCTCT GATACAGCAA 121 GCTGTGGTAG TCGTCTTTGT CCATGAGGAG TGGCTGCCTT TCCTCCTGGG GTTGCTTCTT 181 CTTCTTCTTC TTCTGAAGGC ATAGCCTACT GCTAAGCCCA GAGAGAGCTG CAGCAATAAC 241 AGCTCCCACC AGTGCTGCCC CAAGAAGCCA TGGCCAGATA CGACTGGCTT GTTCCAAGTA 301 AGGCTCAATA TAATTTCTGT AAAAGCCTGG ATCTGACTCT TGGAGGTAGC TGTAGTCATA 361 TCCCAGATCC TTGGATGTTA TGAAGAAATC ACCATTTCTA TAGAGCGGTA TGAAAGGAAC 421 CATGTAAGAG TCTCTGTTAT GGCCGATAGG TGCATTGGCT TCTGGGTAAA CTTCCAAAAG 481 AGGGCGGTGC CTTCGCAGCC ATTGTTCAAA AATACTGTCC ACAAAAGCAT GGTGAAGAAG 541 AAAAATGGGA TCGTTGGCCG ATCCCTGTAC TTGGGACATT GTTCCATTCA TAAAGATATG 601 TAAGGCATTG TGCATGCTAC TTTGAGAAGG ATCTGCTATC CCTGTGAGTG GACTGGCAAA 661 TCCTTCCAGT GTGTTTCTAA AGCTGAAATT GGCAGTTCTA TCCATTGATC CAGATTCATA 721 CTGGGTCAAA CTCAGACAAA ATTCCACATC TGCTGAAGAT GGGAGCCTGG GGGTTTTGGC 781 TTTGTCATGG TTTCCAGGAT TACGTAATAG TGGTCCCTCA GTGTTCCATC GCATAAAACC 841 TGATGGCTAT TATACTCTTC TGATCTGCTA CAAATGATCT GCCAGGAGGA GAAGAAGGAT 901 GCTGGGCTGA GTAAGTTAGG ATTTTCAGGG TGACGACCTC CCAAGTACTC ATCTGTGCAA 961 ATGTCACAGA TTTCTGCATC TCTCCCAATC CCAGTATGGA AACAGTGAAG TTTCTCATCC 1021 CCAGTTTAGT TCTTCGAAAT TTCTTGTTCC CACCAATAAA CAAGAAAAAG TCTGTGCTAG 1081 GCAGAAACCT GGGTGCTCAT GGCCAAAATT CCAATGTTCC CTCCATATTT TCAGAAAGCC 1141 CCCCCCCGAG // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Ishida, M., M-INK, a novel tool for visualizing melanosomes and melanocores. J. Biochem. 161 (4): 323-326 (2017). PMID 28096452. [link to RRC of NBRP] |
---|
Featured content