Resource data sheet
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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEF-Tyrosinase-FLAG (#RDB14635)

Expression vector of mouse Tyrosinase with C-terminal FLAG-tag under the control of the EF1 alpha promoter.

Clone info. Expression vector of mouse Tyrosinase with C-terminal FLAG-tag under the control of the EF1 alpha promoter.
Comment Please refer Kanno, E. et al. Traffic, 11 (4): 491-507, 2010 (PMID 20070612) for pEF-FLAG vector. Expression was confirmed by the deposotor with immunoprecipitation and microscopy.
Vector backbone pEF-FLAG (plasmid)
Size of vector backbone 5.7 kb
Selectable markers Amp^r
Gene/insert name Mouse Tyr cDNA
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ishida, M. et al., J. Biochem. 161 (4): 323-326, 2017)
Additional terms and conditions:
Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the pEF-BOS and pEF-BOS-EX vectors (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Ishida, M. et al., J. Biochem. 161 (4): 323-326, 2017)。
MTAに書く付加的使用条件:
pEF-BOS and pEF-BOS-EXベクタについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクタを商業的に利用する場合、利用者は長田重一博士に連絡するものとする。利用者は、研究成果の公表にあたってpEF-BOSおよびpEF-BOS-EXベクタの文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990)を引用し、長田重一教授への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14635 pEF-Tyrosinase-FLAG DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEF-Tyrosinase-FLAG was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14635).

Reference section:

Ishida, M., Marubashi, S., Fukuda, M., M-INK, a novel tool for visualizing melanosomes and melanocores. J. Biochem. 161 (4): 323-326 (2017). PMID 28096452. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14635_A7C1p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F ()
Sequence file: RDB14635_A7C1a.seq check
>D04384A4_A7C1_2_EF1a-C_F_C03_09_ABI24.ab1
    1 TTATTTGCAT TGTCTTTTTG AGTTTGGATC TTGGTTCATT CTCAAGCCTC AGACAGTGGT
   61 TCAAAGTTTT TTTCTTCCAT TTCAGGTGTC GTGAGGAATT CTCTAGAGCG GCCGCTCTAG
  121 AACTAGTGGA TCCATGTTCT TGGCTGTTTT GTATTGCCTT CTGTGGAGTT TCCAGATCTC
  181 TGATGGCCAT TTTCCTCGAG CCTGTGCCTC CTCTAAGAAC TTGTTGGCAA AAGAATGCTG
  241 CCCACCATGG ATGGGTGATG GGAGTCCCTG CGGCCAGCTT TCAGGCAGAG GTTCCTGCCA
  301 GGATATCCTT CTGTCCAGTG CACCATCTGG ACCTCAGTTC CCCTTCAAAG GGGTGGATGA
  361 CCGTGAGTCC TGGCCCTCTG TGTTTTATAA TAGGACCTGC CAGTGCTCAG GCAACTTCAT
  421 GGGTTTCAAC TGCGGAAACT GTAAGTTTGG ATTTGGGGGC CCAAATTGTA CAGAGAAGCG
  481 AGTCTTGATT AGAAGAAACA TTTTTGATTT GAGTGTCTCC GAAAAGAATA AGTTCTTTTC
  541 TTACCTCACT TTAGCAAAAC ATACTATCAG CTCAGTCTAT GTCATCCCCA CAGGCACCTA
  601 TGGCCAAATG AACAATGGGT CAACACCCAT GTTTAATGAT ATCAACATCT ACGACCTCTT
  661 TGTATGGATG CATTACTATG TGTCAAGGGA CACACTGCTT GGGGGCTCTG AAATATGGAG
  721 GGACATTGAT TTTGCCCATG AAGCACCAGG GTTTCTGCCT TGGCACAGAC TTTTCTTGTT
  781 ATTGTGGGAA CAAGAAATTC GAGAACTAAC TGGGGATGAG AACTTCACTG TTCCATACTG
  841 GGATTGGAGA GATGCAGAAA ACTGTGACAT TTGCACAGAT GAGTACTTGG GAGGTCGTCA
  901 CCCTGAAAAT CCTAACTTAC TCAGCCCAGC ATCCTTCTTC TCCTCCTGGC AGATCATTTG
  961 TAGCAGATCA GAAGAGTATA ATAGCCATCA GGTTTTATGC GATGGAAACA CCCTGAGGGA
 1021 CCACTATTTA CGTAAATCCT GGAAAACCAT GACAAAAGCC CAAAACCCCC CAGGCTCCAT
 1081 CCTCCAGCAG ATGTGATTTG GTCCTGGATT GACCAGTATG AATCTGGAAT TCAAAAGTGG
//
Primer: hGCSF_R ()
Sequence file: RDB14635_A7C1b.seq check
>D04384A4_A7C1_2_hGCSF_R_D03_12_ABI24.ab1
    1 GTGGGGAAAA GGGATCTCTC AAGGGTCCCA GGGTGGGGAC CCTCACTCTA GAGCGGCCGC
   61 CTACTTGTCG TCATCGTCCT TGTAGTCCCG CGGGGTACCC AGATGGCTCT GATACAGCAA
  121 GCTGTGGTAG TCGTCTTTGT CCATGAGGAG TGGCTGCCTT TCCTCCTGGG GTTGCTTCTT
  181 CTTCTTCTTC TTCTGAAGGC ATAGCCTACT GCTAAGCCCA GAGAGAGCTG CAGCAATAAC
  241 AGCTCCCACC AGTGCTGCCC CAAGAAGCCA TGGCCAGATA CGACTGGCTT GTTCCAAGTA
  301 AGGCTCAATA TAATTTCTGT AAAAGCCTGG ATCTGACTCT TGGAGGTAGC TGTAGTCATA
  361 TCCCAGATCC TTGGATGTTA TGAAGAAATC ACCATTTCTA TAGAGCGGTA TGAAAGGAAC
  421 CATGTAAGAG TCTCTGTTAT GGCCGATAGG TGCATTGGCT TCTGGGTAAA CTTCCAAAAG
  481 AGGGCGGTGC CTTCGCAGCC ATTGTTCAAA AATACTGTCC ACAAAAGCAT GGTGAAGAAG
  541 AAAAATGGGA TCGTTGGCCG ATCCCTGTAC TTGGGACATT GTTCCATTCA TAAAGATATG
  601 TAAGGCATTG TGCATGCTAC TTTGAGAAGG ATCTGCTATC CCTGTGAGTG GACTGGCAAA
  661 TCCTTCCAGT GTGTTTCTAA AGCTGAAATT GGCAGTTCTA TCCATTGATC CAGATTCATA
  721 CTGGGTCAAA CTCAGACAAA ATTCCACATC TGCTGAAGAT GGGAGCCTGG GGGTTTTGGC
  781 TTTGTCATGG TTTCCAGGAT TACGTAATAG TGGTCCCTCA GTGTTCCATC GCATAAAACC
  841 TGATGGCTAT TATACTCTTC TGATCTGCTA CAAATGATCT GCCAGGAGGA GAAGAAGGAT
  901 GCTGGGCTGA GTAAGTTAGG ATTTTCAGGG TGACGACCTC CCAAGTACTC ATCTGTGCAA
  961 ATGTCACAGA TTTCTGCATC TCTCCCAATC CCAGTATGGA AACAGTGAAG TTTCTCATCC
 1021 CCAGTTTAGT TCTTCGAAAT TTCTTGTTCC CACCAATAAA CAAGAAAAAG TCTGTGCTAG
 1081 GCAGAAACCT GGGTGCTCAT GGCCAAAATT CCAATGTTCC CTCCATATTT TCAGAAAGCC
1141 CCCCCCCGAG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Ishida, M., M-INK, a novel tool for visualizing melanosomes and melanocores. J. Biochem. 161 (4): 323-326 (2017). PMID 28096452. [link to RRC of NBRP]