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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEF-Myc-Kif1c-tail-879-1100

Expression vector of mature melanosome marker, which Myc-tagged C terminal half tail region of the mouse Kif1c is expressed.

Catalog number RDB14633
Resource name pEF-Myc-Kif1c-tail-879-1100
Clone info. Expression vector of mature melanosome marker, which Myc-tagged C terminal half tail region (amino acid residues 879-1110) of the mouse kinesin superfamily protein, member 1C (Kif1c) is expressed under the control of the EF1 alpha promoter. Please refer Ishida, M., Sci. Rep. 5, 8238, 2015 (PMID 25649263) for Kif1c-tail domain.
Please refer Mori, Y. et al. J. Biol. Chem., 287 (12): 8963-8973, 2012 (PMID 22291024) for pEF-Myc vector. Expression was confirmed by the deposotor with immunoprecipitation and microscopy.
Vector backbone pEF-Myc (plasmid)
Size of vector backbone 5.7 kb
Selectable markers Amp^r
Gene/insert name Mouse Kif1c cDNA
Depositor|Developer Fukuda, Mitsunori |
 
Other clones in our bank mouse Kif1c (NCBI Gene 16562) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ishida, M. et al., J. Biochem. 161 (4): 323-326, 2017)
Additional terms and conditions for distribution Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the pEF-BOS and pEF-BOS-EX vectors (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
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RDB14633 pEF-Myc-Kif1c-tail-879-1100 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Ishida, M., M-INK, a novel tool for visualizing melanosomes and melanocores. J. Biochem. 161 (4): 323-326 (2017). PMID 28096452.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dnaconda's recommendation EXR0070e (English text)
Featured content Dnaconda's recommendation EXR0070j (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14633_A7C1p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F
Region: EF1alpha pro, Myc, insert, hG-CSF p(A)
Sequence file: RDB14633_A7C1a.seq check
>D04384A2_A7C1_2_EF1a-C_F_G01_19_ABI24.ab1
    1 CCATGGATGA CTTTTTGAGT TTGGATCTTG GTTCATTCTC AAGCCTCAGA CAGTGGTTCA
   61 AAGTTTTTTT CTTCCATTTC AGGTGTCGTG AGGAATTCTC TAGAGCGGCC GCTCTAGAAC
  121 TAGTGGATCT GCCACCATGG AGCAAAAGCT CATTTCTGAA GAGGACTTGA ATGAAGGATC
  181 TGATGATAAT GACGAGTCTG GTTTGGTCAC CTGGGCCCCA CCGGAAGGGC CAGAAGCAGT
  241 AGAGGAGACA GTGCCCAACG ACCACTCACC AGCTGTCCGG CCCACCTCAC CACCTCTGTC
  301 CAGCTGGGAG CGGGTGTCGA GATTGATGGA AGAGGACCCT GCCTTCCGTC GCGGCCGCCT
  361 TCGCTGGCTC AAGCAGGAGC AGCTGCGGCT GCAGGGACTG CAGGGCGCTG GGGGCCGGGG
  421 TGGGGGACTG CGCAGGCCTC CAGCCCGCTT TGTGCCCCCG CATGATTGCA AGCTGCGCTT
  481 CCCCTTCAAA AGCAACCCTC AGCACAGGGA GTCCTGGCCA GGAATGGGGA GTGGAGAGGC
  541 CCCAGCTCCT CAGCCCCCTG AAGAGGTCAC TGTCCCCCCA GCACCCCCTA ACCGTAGGCC
  601 TCCAAGTCCC CGAAGACCCC ACCGTTCTCG CAGGAATTCC CTAGATGGAG GGAGCCGATC
  661 TCGGGGAGGG GGTTCTACAC AGCCAGAACC CCAGCACTTA CGGCCCCAAA AGCACAACGG
  721 TTATCCCCAA CAGCCCCAGC CCAGCCCAGC CCAGCGGCCA GGGCCCCGCT ACCCACCATA
  781 CACTACCCCT CCGCGAATGC GACGGCAGCG CTCAGCCCCT GACCTCAAAG AGAGTGGGGC
  841 AGCTGTGTGA AATCACTAGT GAATTCGCGG CCGCCTGCAG GTCGACTAAA TCGAATTCCC
  901 GCGGCCGCTC TAGAGTGAGG GTCCCCACCT GGGACCCTTG AGAGTATCAG TCTCCCACGT
  961 GGGAGACAGA ATCCCTGTTT TATATTTAAA CAGCAGTGTT CCCCATCTGG GTCCTTGCAC
 1021 CCCCTCACTC TGGGCTCAGC GACTGCACAG CGGGCCCTGC AATCCGTGAC CTGTGAGTCC
1081 ATGGACAGTG AGTTACAGAG CCTGGAGGCA TGGATCTTGA G
//
Primer: hGCSF_R
Region: hG-CSF p(A), insert Myc, EF1alpha pro
Sequence file: RDB14633_A7C1b.seq check
>D04384A2_A7C1_2_hGCSF_R_H01_22_ABI24.ab1
    1 GGGGGAACTG ATCTCTCAGG GTCCCAGGTG GGGACCCTCA CTCTAGAGCG GCCGCGGGAA
   61 TTCGATTTAG TCGACCTGCA GGCGGCCGCG AATTCACTAG TGATTTCACA CAGCTGCCCC
  121 ACTCTCTTTG AGGTCAGGGG CTGAGCGCTG CCGTCGCATT CGCGGAGGGG TAGTGTATGG
  181 TGGGTAGCGG GGCCCTGGCC GCTGGGCTGG GCTGGGCTGG GGCTGTTGGG GATAACCGTT
  241 GTGCTTTTGG GGCCGTAAGT GCTGGGGTTC TGGCTGTGTA GAACCCCCTC CCCGAGATCG
  301 GCTCCCTCCA TCTAGGGAAT TCCTGCGAGA ACGGTGGGGT CTTCGGGGAC TTGGAGGCCT
  361 ACGGTTAGGG GGTGCTGGGG GGACAGTGAC CTCTTCAGGG GGCTGAGGAG CTGGGGCCTC
  421 TCCACTCCCC ATTCCTGGCC AGGACTCCCT GTGCTGAGGG TTGCTTTTGA AGGGGAAGCG
  481 CAGCTTGCAA TCATGCGGGG GCACAAAGCG GGCTGGAGGC CTGCGCAGTC CCCCACCCCG
  541 GCCCCCAGCG CCCTGCAGTC CCTGCAGCCG CAGCTGCTCC TGCTTGAGCC AGCGAAGGCG
  601 GCCGCGACGG AAGGCAGGGT CCTCTTCCAT CAATCTCGAC ACCCGCTCCC AGCTGGACAG
  661 AGGTGGTGAG GTGGGCCGGA CAGCTGGTGA GTGGTCGTTG GGCACTGTCT CCTCTACTGC
  721 TTCTGGCCCT TCCGGTGGGG CCCAGGTGAC CAAACCAGAC TCGTCATTAT CATCAGATCC
  781 TTCATTCAAG TCCTCTTCAG AAATGAGCTT TTGCTCCATG GTGGCAGATC CACTAGTTCT
  841 AGAGCGGCCG CTCTAGAGAA TTCCTCACGA CACCTGAAAT GGAAGAAAAA AAACTTTGAA
  901 CCACTGTCTG AGGCTTGAGA ATGAACCAAG ATCCAAACTC AAAAAGGGCA AATTCCAAGG
  961 AGAAATTACA TCAAGTGCCC AAGCTGGCCT AACTTCAGTC TCCACCCCCA CTCAGTGTGG
 1021 GAACTCCATC GCATAAAAAC CCCTCCCCCC CAACCTAAAG ACGACGTACT TCCAAAAGGC
 1081 TCGAGAACTA TCGAGTGACT GGAACGCGCC GGTACTCCGT GGAGTCACAT AGAAGCGAAC
1141 CGGGCC
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Ishida, M., M-INK, a novel tool for visualizing melanosomes and melanocores. J. Biochem. 161 (4): 323-326 (2017). PMID 28096452.

2020.10.02

GNP_filter3_RDBDEP_html_200316.pl