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pEF-Myc-Kif1c-tail-879-1100 (#RDB14633)

Expression vector of mature melanosome marker, which Myc-tagged C terminal half tail region of the mouse Kif1c is expressed.

Clone info. Expression vector of mature melanosome marker, which Myc-tagged C terminal half tail region (amino acid residues 879-1110) of the mouse kinesin superfamily protein, member 1C (Kif1c) is expressed under the control of the EF1 alpha promoter. Please refer Ishida, M., Sci. Rep. 5, 8238, 2015 (PMID 25649263) for Kif1c-tail domain.
Comment Please refer Mori, Y. et al. J. Biol. Chem., 287 (12): 8963-8973, 2012 (PMID 22291024) for pEF-Myc vector. Expression was confirmed by the deposotor with immunoprecipitation and microscopy.
Vector backbone pEF-Myc (plasmid)
Size of vector backbone 5.7 kb
Selectable markers Amp^r
Gene/insert name Mouse Kif1c cDNA
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ishida, M. et al., J. Biochem. 161 (4): 323-326, 2017)
Additional terms and conditions:
Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the pEF-BOS and pEF-BOS-EX vectors (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Ishida, M. et al., J. Biochem. 161 (4): 323-326, 2017)。
MTAに書く付加的使用条件：
pEF-BOS and pEF-BOS-EXベクタについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクタを商業的に利用する場合、利用者は長田重一博士に連絡するものとする。利用者は、研究成果の公表にあたってpEF-BOSおよびpEF-BOS-EXベクタの文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990)を引用し、長田重一教授への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14633 pEF-Myc-Kif1c-tail-879-1100 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEF-Myc-Kif1c-tail-879-1100 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14633).

Reference section:

Ishida, M., Marubashi, S., Fukuda, M., M-INK, a novel tool for visualizing melanosomes and melanocores. J. Biochem. 161 (4): 323-326 (2017). PMID 28096452. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14633_A7C1p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168)
Region: EF1alpha pro, Myc, insert, hG-CSF p(A)
Sequence file: RDB14633_A7C1a.seq check
>D04384A2_A7C1_2_EF1a-C_F_G01_19_ABI24.ab1
    1 CCATGGATGA CTTTTTGAGT TTGGATCTTG GTTCATTCTC AAGCCTCAGA CAGTGGTTCA
   61 AAGTTTTTTT CTTCCATTTC AGGTGTCGTG AGGAATTCTC TAGAGCGGCC GCTCTAGAAC
  121 TAGTGGATCT GCCACCATGG AGCAAAAGCT CATTTCTGAA GAGGACTTGA ATGAAGGATC
  181 TGATGATAAT GACGAGTCTG GTTTGGTCAC CTGGGCCCCA CCGGAAGGGC CAGAAGCAGT
  241 AGAGGAGACA GTGCCCAACG ACCACTCACC AGCTGTCCGG CCCACCTCAC CACCTCTGTC
  301 CAGCTGGGAG CGGGTGTCGA GATTGATGGA AGAGGACCCT GCCTTCCGTC GCGGCCGCCT
  361 TCGCTGGCTC AAGCAGGAGC AGCTGCGGCT GCAGGGACTG CAGGGCGCTG GGGGCCGGGG
  421 TGGGGGACTG CGCAGGCCTC CAGCCCGCTT TGTGCCCCCG CATGATTGCA AGCTGCGCTT
  481 CCCCTTCAAA AGCAACCCTC AGCACAGGGA GTCCTGGCCA GGAATGGGGA GTGGAGAGGC
  541 CCCAGCTCCT CAGCCCCCTG AAGAGGTCAC TGTCCCCCCA GCACCCCCTA ACCGTAGGCC
  601 TCCAAGTCCC CGAAGACCCC ACCGTTCTCG CAGGAATTCC CTAGATGGAG GGAGCCGATC
  661 TCGGGGAGGG GGTTCTACAC AGCCAGAACC CCAGCACTTA CGGCCCCAAA AGCACAACGG
  721 TTATCCCCAA CAGCCCCAGC CCAGCCCAGC CCAGCGGCCA GGGCCCCGCT ACCCACCATA
  781 CACTACCCCT CCGCGAATGC GACGGCAGCG CTCAGCCCCT GACCTCAAAG AGAGTGGGGC
  841 AGCTGTGTGA AATCACTAGT GAATTCGCGG CCGCCTGCAG GTCGACTAAA TCGAATTCCC
  901 GCGGCCGCTC TAGAGTGAGG GTCCCCACCT GGGACCCTTG AGAGTATCAG TCTCCCACGT
  961 GGGAGACAGA ATCCCTGTTT TATATTTAAA CAGCAGTGTT CCCCATCTGG GTCCTTGCAC
 1021 CCCCTCACTC TGGGCTCAGC GACTGCACAG CGGGCCCTGC AATCCGTGAC CTGTGAGTCC
1081 ATGGACAGTG AGTTACAGAG CCTGGAGGCA TGGATCTTGA G
//
Primer: hGCSF_R (Pr0031)
Region: hG-CSF p(A), insert Myc, EF1alpha pro
Sequence file: RDB14633_A7C1b.seq check
>D04384A2_A7C1_2_hGCSF_R_H01_22_ABI24.ab1
    1 GGGGGAACTG ATCTCTCAGG GTCCCAGGTG GGGACCCTCA CTCTAGAGCG GCCGCGGGAA
   61 TTCGATTTAG TCGACCTGCA GGCGGCCGCG AATTCACTAG TGATTTCACA CAGCTGCCCC
  121 ACTCTCTTTG AGGTCAGGGG CTGAGCGCTG CCGTCGCATT CGCGGAGGGG TAGTGTATGG
  181 TGGGTAGCGG GGCCCTGGCC GCTGGGCTGG GCTGGGCTGG GGCTGTTGGG GATAACCGTT
  241 GTGCTTTTGG GGCCGTAAGT GCTGGGGTTC TGGCTGTGTA GAACCCCCTC CCCGAGATCG
  301 GCTCCCTCCA TCTAGGGAAT TCCTGCGAGA ACGGTGGGGT CTTCGGGGAC TTGGAGGCCT
  361 ACGGTTAGGG GGTGCTGGGG GGACAGTGAC CTCTTCAGGG GGCTGAGGAG CTGGGGCCTC
  421 TCCACTCCCC ATTCCTGGCC AGGACTCCCT GTGCTGAGGG TTGCTTTTGA AGGGGAAGCG
  481 CAGCTTGCAA TCATGCGGGG GCACAAAGCG GGCTGGAGGC CTGCGCAGTC CCCCACCCCG
  541 GCCCCCAGCG CCCTGCAGTC CCTGCAGCCG CAGCTGCTCC TGCTTGAGCC AGCGAAGGCG
  601 GCCGCGACGG AAGGCAGGGT CCTCTTCCAT CAATCTCGAC ACCCGCTCCC AGCTGGACAG
  661 AGGTGGTGAG GTGGGCCGGA CAGCTGGTGA GTGGTCGTTG GGCACTGTCT CCTCTACTGC
  721 TTCTGGCCCT TCCGGTGGGG CCCAGGTGAC CAAACCAGAC TCGTCATTAT CATCAGATCC
  781 TTCATTCAAG TCCTCTTCAG AAATGAGCTT TTGCTCCATG GTGGCAGATC CACTAGTTCT
  841 AGAGCGGCCG CTCTAGAGAA TTCCTCACGA CACCTGAAAT GGAAGAAAAA AAACTTTGAA
  901 CCACTGTCTG AGGCTTGAGA ATGAACCAAG ATCCAAACTC AAAAAGGGCA AATTCCAAGG
  961 AGAAATTACA TCAAGTGCCC AAGCTGGCCT AACTTCAGTC TCCACCCCCA CTCAGTGTGG
 1021 GAACTCCATC GCATAAAAAC CCCTCCCCCC CAACCTAAAG ACGACGTACT TCCAAAAGGC
 1081 TCGAGAACTA TCGAGTGACT GGAACGCGCC GGTACTCCGT GGAGTCACAT AGAAGCGAAC
1141 CGGGCC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ishida, M., M-INK, a novel tool for visualizing melanosomes and melanocores. J. Biochem. 161 (4): 323-326 (2017). PMID 28096452. [link to RRC of NBRP]