Resource data sheet
Prev.

pFBD-Hs alpha1(K40R) CFXaGH/beta3 CFXaGF

Expression vector of His tagged human tubulin alpha-1(mutant, K40R, constitutive active form) and Flag tagged human tubulin beta-3.

Catalog number RDB14531
Resource name pFBD-Hs alpha1(K40R) CFXaGH/beta3 CFXaGF
Alternative name Fxa_His- and Fxa_FLAG-tagged Human alpha1(K40R) bata3 tubulin
Clone info. Expression vector of His tagged human tubulin alpha-1(mutant, K40R, constitutive active form) and Flag tagged human tubulin beta-3. This clone is driven by the two promoters (PPH and Pp10) in a single vector for expression of two proteins simultaneously.
Unpublished plasmid. Reference sequence: NP_006073 for TUBA1B, NP_006077 for TUBB3.
Vector backbone pFastBac Dual (Plasmid)
Size of vector backbone 5.2 kb
Selectable markers Amp^r (E. coli), Gent^r.
Gene/insert name Human TUBA1B cDNA Human TUBB3 cDNA
Depositor Muto, Etsuko |
 
Other clones in our bank human TUBA1B (NCBI Gene 10376) | human TUBB3 (NCBI Gene 10381) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not- for profit organization for a not-for-profit research. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR (Minoura, I. et al. FEBS Lett. 587, 3450-3455, 2013) and an acknowledgment to the DEPOSITOR are requested. The BIOLOGICAL RESOURCE will not be further distributed to others without the DEPOSITOR's written consent. RECIPIENT must contact the DEPOSITOR in the case of application for any patents based on the results from the use of the BIOLOGICAL RESOURCE.
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RDB14531 pFBD-Hs alpha1(K40R) CFXaGH/beta3 CFXaGF DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Purification of Recombinant Tubulin (pdf)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14531_A7A5p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Region: polyhedrin pro, L21, insert2-5' | Primer: pPolyhedrin_F | Sequence: RDB14531_A7A5g.seq
>D03763A2_A7A5_1_pPolyhedrin_F_F03_18_ABI24.ab1
    1 CATTTTACTG TTTTCGTACA GTTTTGTAAT AAAAAAACCT ATAAATATTC CGGATTATTC
   61 ATACCGTCCC ACCATCGGGC GCGGATCCCG GTCCGAAGCG CGCGGAATTC AAAGGCCTAC
  121 GTCGACGAGC TCACTAGTAA CTCCTAAAAA ACCGCCACCA TGCGCGAGTG CATCTCCATC
  181 CACGTGGGCC AGGCTGGTGT CCAGATCGGC AACGCTTGCT GGGAGCTGTA CTGCCTGGAG
  241 CACGGTATCC AGCCCGACGG CCAGATGCCC TCCGACCGCA CCATCGGTGG CGGCGACGAC
  301 TCCTTCAACA CCTTCTTCTC CGAAACCGGT GCTGGCAAGC ACGTGCCCCG CGCTGTGTTC
  361 GTGGATCTGG AACCCACCGT GATCGACGAA GTGCGTACCG GCACCTACCG TCAGCTGTTC
  421 CACCCCGAGC AACTGATCAC CGGCAAGGAG GACGCTGCTA ACAACTACGC TCGTGGTCAC
  481 TACACCATCG GCAAGGAAAT CATCGACCTG GTCCTGGACC GTATCCGCAA GCTGGCTGAC
  541 CAGTGCACCG GCCTGCAGGG ATTCCTCGTG TTCCACTCCT TCGGTGGTGG AACCGGTTCT
  601 GGTTTCACCT CCCTGCTGAT GGAACGTCTG TCTGTGGACT ACGGCAAGAA GTCCAAGTTG
  661 GAGTTCTCCA TCTACCCCGC TCCCCAGGTG TCCACCGCTG TGGTCGAGCC CTACAACTCC
  721 ATCCTGACCA CCCACACCAC CCTGGAACAC TCCGACTGCG CTTTCATGGT GGACAACGAG
  781 GCTATCTACG ACATCTGCCG TCGTAACCTG GACATCGAGC GTCCCACCTA CACCAACCTG
  841 AACCGTCTGA TCTCCCAGAT CGTGTCCTCC ATCACCGCTT CCCTGCGTTT CGACGGTGCT
  901 CTGAACGTGG ACCTGACCGA GTTCCAGACC AACCTGGTGC CCTACCCCCC GTATCCACTT
  961 TCCCCCTGGC TACCTACGCT CCCGTGATCT CCCGCTGAGA AGGCTTTACC CACGAGGCAG
 1021 CCTGGTCGTG GCTGAAGATC ACCAAACGTC TTGCCTTTCG AGCCGCTACC AGAATTGATC
 1081 CAGTTGCCGA ACCCTCGGTC ACGGCAAGTT ACCATGTCTG ACATGGCTAC CTGTATATAC
1141 G
//
Region: Tn7L, 8xHis, Factor Xa, insert2-3' | Primer: SV40polyA-R | Sequence: RDB14531_A7A5h.seq
>D03763A2_A7A5_1_SV40polyA_R_B06_02_ABI08.ab1
    1 AAAAAAGTGG GAGTTTTTAA GCAGTAAAAC CTCTACAAAT GTGGTATGGC TGATTATGAT
   61 CCTCTAGTAC TTCTCGACAA GCTTTTAGTG GTGATGGTGG TGATGATGGT GACCACCGGA
  121 ACCACCACGA CCCTCGATGT ATTCCTCGCC TTCCTCTTCG CCCTCGCCCT CGACGGAGTC
  181 GACGCCCACT TCCTCGTAGT CCTTTTCCAG AGCAGCCATG TCCTCGCGAG CCTCGGAGAA
  241 CTCGCCCTCT TCCATGCCTT CGCCCACGTA CCAGTGCACG AAAGCGCGCT TAGCGTACAT
  301 CAGGTCGAAC TTGTGGTCCA GACGAGCCCA AGCCTCAGCG ATAGCGGTGG TGTTGGACAG
  361 CATGCACACA GCACGCTGCA CCTTAGCCAA GTCGCCACCG GGCACCACAG TGGGGGGCTG
  421 GTAGTTGATT CCGACCTTGA AACCGGTGGG GCACCAGTCC ACGAACTGGA TGGAACGCTT
  481 GGTCTTGATG GTAGCGATAG CAGCGTTCAC GTCCTTAGGC ACCACGTCGC CACGGTACAG
  541 CAGGCAGCAA GCCATGTACT TGCCGTGACG GGGGTCGCAC TTGACCATCT GGTTAGCGGG
  601 CTCGAAGCAG GCGTTGGTGA TCTCAGCCAC GGACAGCTGC TCGTGGTAAG CCTTCTCAGC
  661 GGAGATCACG GGAGCGTAGG TAGCCAGGGG GAAGTGGATA CGGGGGTAGG GCACCAGGTT
  721 GGTCTGGAAC TCGGTCAGGT CCACGTTCAG AGCACCGTCG AAACGCAGGG AAGCGGTGAT
  781 GGAGGACACG ATCTGGGAGA TCAGACGGTT CAGGTTGGTG TAGGTGGGAC GCTCGATGTC
  841 CAGGTTACGA CGGCAGATGT CGTAGATAGC CTCGTTGTCC ACCATGAAAG CGCAGTCGGA
  901 GTGTTCCAGG GTGGTGTGGG TGGTCAGGAT GGAGTTGTAG GGCTCGACCA CAGCGGTGGA
  961 CACCTGGGGA GCGGGGTAGA TGGAGAACTC CAACTTGGAC TTCTTGCCGT AGTCCACAGA
 1021 CAGACGTTCA TCAGCCAGGG AGGTGAAAAC CAGAGCGGGT TCACACGCCG AGTGGAACAC
 1081 GAAGAATCCT GCAAGCGTGC ACTGGTCAGC CAGCTGCGAT ACGTTCAAGA ACAAGGTCGA
1141 ATGAATTTCC TGTGCCGAG
//
Region: p10pro, L21, insert1-5' | Primer: pP10_F | Sequence: RDB14531_A7A5i.seq
>D03763A2_A7A5_1_pP10_F_C06_03_ABI08.ab1
    1 ATTATAGGAT TATTATCAAA TCATTTGTAT ATTAATTAAA ATACTATACT GTAAATTACA
   61 TTTTATTTAC AATCACTCGA CGAAGACTTG ATCACCCGGG ATCTCGAGAA CTCCTAAAAA
  121 ACCGCCACCA TGCGCGAGAT CGTGCACATC CAGGCTGGTC AGTGCGGTAA CCAGATCGGT
  181 GCTAAGTTCT GGGAGGTCAT CTCCGACGAG CACGGTATCG ACCCTTCCGG TAACTACGTG
  241 GGTGACTCCG ACCTGCAGCT GGAGCGCATT TCCGTGTACT ACAACGAAGC CTCCTCCCAC
  301 AAATACGTGC CTCGCGCTAT CCTGGTGGAC CTGGAGCCTG GTACTATGGA CTCTGTGCGC
  361 TCCGGTGCTT TCGGTCACCT GTTCCGCCCT GACAACTTCA TCTTCGGTCA GTCCGGTGCT
  421 GGTAACAACT GGGCTAAGGG TCACTACACC GAGGGTGCTG AGCTCGTCGA CTCCGTGCTG
  481 GACGTGGTGC GCAAGGAGTG CGAGAACTGC GACTGCCTGC AGGGTTTCCA GCTGACTCAC
  541 TCCCTGGGTG GTGGTACCGG ATCTGGTATG GGTACCCTGC TGATCTCCAA GGTGCGCGAG
  601 GAGTACCCTG ACCGCATCAT GAATACCTTC TCCGTCGTGC CTTCCCCTAA GGTGTCCGAC
  661 ACCGTGGTGG AGCCTTACAA CGCTACCCTG TCCATCCACC AACTGGTGGA GAACACCGAC
  721 GAGACCTACT GCATCGACAA CGAGGCTCTG TACGACATCT GCTTCCGCAC CCTGAAGCTG
  781 GCTACCCCTA CCTACGGTGA CCTGAACCAC CTGGTCTCCG CTACCATGTC CGGTGTGACC
  841 ACCTCCCTGC GCTTCCCTGG TCAGCTGAAC GCTGACCTGC GCAAGCTGGC TGTGAACATG
  901 GTGCCTTTCC CTCGCCTGCA CTTCTTCATG CCTGGTTTCG CTCCTCTGAC CGCTCGCGGT
  961 TCCCAGCAAT ACCGCGCTCT GACCGTGCCT GAGCTGACCC AGCAGATGTT CGACGCTAAG
 1021 AACATGATGG GCTGCTTGCG ACCCTCGCCA CGGTCGCTAC CTGACCGTGG CTACCGTGTT
 1081 CCGCGGTCGC ATGTCATGAA GGAGGTGGAC GAGCCAGATG CCTGGCCTAT CAAGTCCAGA
1141 CTCCTCCTAC CTTTCGTGGG AGAGTTGGAA TTTC
//
Region: FLAG,Factor Xa,insert1-3' | Primer: HSV_TK_pA_R | Sequence: RDB14531_A7A5j.seq
>D03763A2_A7A5_1_HSV_TK_pA_R_D07_04_ABI08.ab1
    1 AGCGGGATCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC ATCTCCCGGT
   61 ACCGCATGCT ATGCATCAGC TGCTAGCTTA CTTGTCATCG TCATCTTTGT AGTCACCACC
  121 GGAACCACCA CGACCCTCGA TCTTAGGACC CTGAGCCTCG GACTCTTCCT CATCGTCCTC
  181 GTACATCTCA CCCTCTTCTT CAGCGGTAGC GTCCTGGTAC TGCTGGTACT CGGACACCAG
  241 GTCGTTCATG TTGGACTCAG CCTCGGTGAA CTCCATCTCG TCCATACCCT CACCGGTGTA
  301 CCAGTGCAGG AAAGCCTTGC GGCGGAACAT AGCGGTGAAC TGCTCGGAGA TGCGCTTGAA
  361 CAGCTCCTGG ATAGCGGTGG AGTTACCGAT GAAGGTGGAG GACATCTTCA GACCGCGAGG
  421 AGGGATGTCG CACACAGCCA CCTTCACGTT GTTAGGGATC CACTCCACGA AGTAGGAGGA
  481 GTTCTTGGAC TGGATAGCCA GCATCTGCTC GTCCACCTCC TTCATGGACA TGCGACCGCG
  541 GAACACGGTA GCCACGGTCA GGTAGCGACC GTGGCGAGGG TCGCAAGCAG CCATCATGTT
  601 CTTAGCGTCG AACATCTGCT GGGTCAGCTC AGGCACGGTC AGAGCGCGGT ATTGCTGGGA
  661 ACCGCGAGCG GTCAGAGGAG CGAAACCAGG CATGAAGAAG TGCAGGCGAG GGAAAGGCAC
  721 CATGTTCACA GCCAGCTTGC GCAGGTCAGC GTTCAGCTGA CCAGGGAAGC GCAGGGAGGT
  781 GGTCACACCG GACATGGTAG CGGAGACCAG GTGGTTCAGG TCACCGTAGG TAGGGGTAGC
  841 CAGCTTCAGG GTGCGGAAGC AGATGTCGTA CAGAGCCTCG TTGTCGATGC AGTAGGTCTC
  901 GTCGGTGTTC TCCACCAGTT GGTGGATGGA CAGGGTAGCG TTGTAAAGGC TCCACCACGG
  961 TGTCGGACAC CTTAGGGAAG GCACGACGGA GAAGGTATTC ATGATGCGGT CAGGGTACTC
 1021 CTCGCGCACC TTGGAGATCA GCAGGTTACC CATACCAGAT CCGGGTACCA CCACCCCAGG
 1081 GAGTGAGTCA GCTGGAAACC TGCAGCAGGT CGCAGTTCTC GCCTCCTGCG CCACCACGGT
1141 TCAGGCAACG GAGTCGGAAC GGAGCCTCAA GCACCCCCCT TCCGGGG
//
Region: GmR, Tn7R | Primer: Pcpro_F | Sequence: RDB14531_A7A5k.seq
>D03763A2_A7A5_1_Pcpro_F_E07_05_ABI08.ab1
    1 GAGACGGGAA GGGGGCTCAC GGCAACTGGG TCCAGAACCT TGACCGAACG CAGCGGTGGT
   61 AACGGCGCAG TGGCGGTTTT CATGGCTTGT TATGACTGTT TTTTTGTACA GTCTATGCCT
  121 CGGGCATCCA AGCAGCAAGC GCGTTACGCC GTGGGTCGAT GTTTGATGTT ATGGAGCAGC
  181 AACGATGTTA CGCAGCAGCA ACGATGTTAC GCAGCAGGGC AGTCGCCCTA AAACAAAGTT
  241 AGGTGGCTCA AGTATGGGCA TCATTCGCAC ATGTAGGCTC GGCCCTGACC AAGTCAAATC
  301 CATGCGGGCT GCTCTTGATC TTTTCGGTCG TGAGTTCGGA GACGTAGCCA CCTACTCCCA
  361 ACATCAGCCG GACTCCGATT ACCTCGGGAA CTTGCTCCGT AGTAAGACAT TCATCGCGCT
  421 TGCTGCCTTC GACCAAGAAG CGGTTGTTGG CGCTCTCGCG GCTTACGTTC TGCCCAAGTT
  481 TGAGCAGCCG CGTAGTGAGA TCTATATCTA TGATCTCGCA GTCTCCGGCG AGCACCGGAG
  541 GCAGGGCATT GCCACCGCGC TCATCAATCT CCTCAAGCAT GAGGCCAACG CGCTTGGTGC
  601 TTATGTGATC TACGTGCAAG CAGATTACGG TGACGATCCC GCAGTGGCTC TCTATACAAA
  661 GTTGGGCATA CGGGAAGAAG TGATGCACTT TGATATCGAC CCAAGTACCG CCACCTAACA
  721 ATTCGTTCAA GCCGAGATCG GCTTCCCGGC CGCGGAGTTG TTCGGTAAAT TGTCACAACG
  781 CCGCGAATAT AGTCTTTACC ATGCCCTTGG CCACGCCCCT CTTTAATACG ACGGGCAATT
  841 TGCACTTCAG AAAATGAAGA GTTTGCTTTA GCCATAACAA AAGTCCAGTA TGCTTTTTCA
  901 CAGCATAACT GGACTGATTT CAGTTTACAA CTATTCTGTC TAGTTTAAGA CTTTATTGTC
  961 ATAGTTTAGA TCTATTTTGT TCAGTTTAAG ACTTTATTGT CCGCCACACC CGCCTTACGC
 1021 AGGGCATCCA TTTAATTACT CAAACCGTAA CCGATTTTGC CCAGGTTACG CGGCTGGTCT
 1081 TATGCCGGGT GTGAATACCG CAACAGATGC GGTAAGGGAG AAAATACCGG CAATCAAGCG
1141 CCTCCTCGGC CTTTCCTCGC CTCAACTTGA ACTCTTCGCC TTGTGGCGC
//
Region: f1ori, Tn7L, SV40p(A), 8xHis, FactorXa, insert2-3' | Primer: f1ori_F | Sequence: RDB14531_A7A5l.seq
>D03763A2_A7A5_1_f1ori_F_H03_24_ABI24.ab1
    1 CGGGGGCTAG GCGCTGGCAA GTGTAGCGGT CACGCTGCGC GTAACCACCA CACCCGCCGC
   61 GCTTAATGCG CCGCTACAGG GCGCGTCCCA TTCGCCATTC AGGCTGCAAA TAAGCGTTGA
  121 TATTCAGTCA ATTACAAACA TTAATAACGA AGAGATGACA GAAAAATTTT CATTCTGTGA
  181 CAGAGAAAAA GTAGCCGAAG ATGACGGTTT GTCACATGGA GTTGGCAGGA TGTTTGATTA
  241 AAAACATAAC AGGAAGAAAA ATGCCCCGCT GTGGGCGGAC AAAATAGTTG GGAACTGGGA
  301 GGGGTGGAAA TGGAGTTTTT AAGGATTATT TAGGGAAGAG TGACAAAATA GATGGGAACT
  361 GGGTGTAGCG TCGTAAGCTA ATACGAAAAT TAAAAATGAC AAAATAGTTT GGAACTAGAT
  421 TTCACTTATC TGGTTCGGAT CTCCTAGGCT CAAGCAGTGA TCAGATCCAG ACATGATAAG
  481 ATACATTGAT GAGTTTGGAC AAACCACAAC TAGAATGCAG TGAAAAAAAT GCTTTATTTG
  541 TGAAATTTGT GATGCTATTG CTTTATTTGT AACCATTATA AGCTGCAATA AACAAGTTAA
  601 CAACAACAAT TGCATTCATT TTATGTTTCA GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA
  661 AAGCAAGTAA AACCTCTACA AATGTGGTAT GGCTGATTAT GATCCTCTAG TACTTCTCGA
  721 CAAGCTTTTA GTGGTGATGG TGGTGATGAT GGTGACCACC GGAACCACCA CGACCCTCGA
  781 TGTATTCCTC GCCTTCCTCT TCGCCCTCGC CCTCGACGGA GTCGACGCCC ACTTCCTCGT
  841 AGTCCTTTTC CAGAGCAGCC ATGTCCTCGC GAGCCTCGGA GAACTCGCCC TCTTCCATGC
  901 CTTCGCCCAC GTACCAGTGC ACGAAAGCGC GCTTAGCGTA CATCAGTCGA ACTTGTGGTC
  961 CAGACGAGCC CAAGCCCTCA GCGATAGCGG TGGTGTTTGG GACAGCATGC ACACAGGCAC
 1021 GGCTGCCACT TTAGCCAAGG TCGGCACCGG CATCACAGTG GGAGCCTGGT AGTTGAATTC
1081 GAACGTTGCA AACCGGATGC G
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

reference Minoura, I., Overexpression, purification, and functional analysis of recombinant human tubulin dimer. FEBS Lett., 587 (21): 3450-3455 (2013). PMID 24021646.

2019.10.10

GNP_filter3_RDBDEP_html_190927.pl